Human Bone Marrow-derived Stem Research Articles

Cytotoxic and anti-metastatic effects of Hypericum perforatum olive oil extract in colorectal cancer cells and human bone marrow derived stem cells

This study is aimed to investigate the effects of Hypericum perforatum olive oil extract on the cytotoxic and metastatic properties of human colorectal cancer cells and human bone marrow-derived mesenchymal stem cells. In addition, ALDH3A1 and Vimentin expressions were evaluated by qRT-PCR and western blot analysis. Total phenolic and flavonoid contents and antioxidant activity of methanol extracts prepared with oil enrichment were measured using spectrophotometry-based methods. The cytotoxic effects of the extracts on SW-480 and bone marrow-derived mesenchymal stem cells were evaluated by MTT assay, resulting in IC50 values of 4.8 mg/ml and 4.9 mg/ml, respectively. It was determined that cell migration and colony formation were significantly reduced at the IC50 values determined for SW-480 and human bone marrow-derived mesenchymal stem cells.

The Effect of TNF-α on the Expression of MMP9 in Human Mesenchymal Bone Marrow-Derived Stem Cells

Matrix metalloproteinase 9 (MMP9) as the enzyme of adult stem cells secreted from damage cells. In spite of low level of MMP9 enzyme in the mesenchymal stem cells, many inflammatory cytokines stimulation such as TNF-α could increase MMP9 level in cells.
 
 Current study evaluated the expression of the MMP9 enzyme under the influence of TNF-α in human bone marrow mesenchymal stem cells.
 
 The human bone marrow mesenchymal stem cells were classified into control and experimental groups. In the experimental groups, various concentrations of the TNF-α (1ng/ml and 10ng/ml) were administrated in different times (10 and 24 hours), whereas the control group was not treated with TNF-α. MMP9 gene expression was evaluated by Real-Time PCR. TNF-α administration in 1ng/ml and 10ng/ml dosage for 10 hours, induced the expression of MMP9 1468.3 and 1782.8 times more than the control group, respectively.
 
 After 24h, in comparison between 1ng/ml and 10ng/ml with control groups, MMP9 expression were 442.64 and 1184.4 times more than control group, respectively.
 
 In conclusion, the expression rate of the MMP9 gene in bone marrow mesenchymal stem cells might be effected by dosage and time of exposure to TNF-α. Furthermore, the time of exposure might have the prominent role in alteration of MMP9 gene expression induction in the mesenchymal stem cells.

The Role of Statins in the Osteogenesis on Bone Fusion Using Bone Marrow-Derived and Adipose-Derived Stem Cells

Introduction Statins are commonly prescribed drugs that inhibit 3-hydroxy-3-methylglutaryl coenzyme A (HMG Co-A) reductase, decreasing hepatic cholesterol biosynthesis by reducing serum cholesterol concentrations. Several studies demonstrated statins enhance new bone formation and increase bone volume in an animal. In this study, simvastatin and lovastatin were employed to elucidate the osteogenic effects of stains on the culture system of two stem cells; human bone marrow-derived (hMSC) and adipose-derived stem cell (hADSC). Materials and Methods In this study, simvastatin and lovastatin were employed to elucidate the osteogenic effects of stains on the culture system of two stem cells; human bone marrow-derived (hMSC) and adipose-derived stem cell (hADSC). There are two experimental conditions: (1) statins supplemented along with other osteoinductive supplements and (2) without them. The statins increased the expression of the osteogenic markers, such as alkaline phosphatase (ALP) activity and alizarin red S staining(ARS) on both conditions. These results were verified with gene mRNA levels using PCR. Finally, ex vivo calvaria organ culture model was investigated by H&E and Masson trichrome staining. Results The statins increased the expression of the osteogenic markers, such as alkaline phosphatase (ALP) activity and alizarin red S staining (ARS) on both conditions. These results were verified with gene mRNA levels using PCR. There were almost the same trends in the expression of osteogenic makers induced from stem cell, although MSC was differentiated slower than ADSC. Also, neonatal murine calvarial was introduced to find the osteogenic effects of statins in ex vivo. It is observed that the bone density and thickness in the dissected calvarial tissue was enhanced in culture system including statins. Conclusion Our results suggests that statins could stimulate the differentiation of stem cell into osteoblasts and also affect the process of bone formation, resulting in the increase of mineralization induced by them. It implies that statins can be candidates as osteoinductive materials for bone fusion Disclosure of Interest None declared

Skeletal (stromal) stem cells – basic biology and clinical use in tissue regeneration

Human bone marrow-derived stromal stem cells [also known as skeletal stem cells or mesenchymal stem cells (hMSCs)] are a group of clonogenic cells that are present among the bone marrow stroma as well as the stroma of other organs. hMSCs are capable of multilineage differentiation into mesoderm-type cells such as osteoblasts, adipocytes and chondrocytes, and possibly, but still controversial, non-mesoderm-type cells (e.g. neuronal cells or hepatocytes). Moreover, hMSCs provide supportive stroma for growth and differentiation of haematopoietic stem cells and haematopoiesis. Recently, hMSCs have been employed in an increasing number of cell-based therapies for treating skeletal and non-skeletal chronic degenerative diseases.

338. Efficient Transduction of Human Bone Marrow-Derived Hematopoietic Stem Cells with Clinical Grade Lentiviral Vectors

338. Efficient Transduction of Human Bone Marrow-Derived Hematopoietic Stem Cells with Clinical Grade Lentiviral Vectors