Human Body Research Articles

Prevalence of Dysmagnesemia among Patients with Diabetes Mellitus and the Associated Health Outcomes: A Cross-Sectional Study

Introduction: Magnesium is a vital intracellular cation crucial for over 320 enzymatic reactions related to energy metabolism, musculoskeletal function, and nucleic acid synthesis and plays a pivotal role in human physiology. This study aimed to explore the prevalence of dysmagnesemia in patients with diabetes mellitus and evaluate its correlations with glycemic control, medication use, and diabetic complications. Methods: A cross-sectional study was conducted at Sultan Qaboos University Hospital, including 316 patients aged 18 years or older with diabetes mellitus. Data included demographics, medical history, medications, and biochemical parameters. Serum total magnesium concentrations were measured, and dysmagnesemia was defined as magnesium ≤ 0.69 mmol/L for hypomagnesemia and ≥1.01 mmol/L for hypermagnesemia. Results: The prevalence of hypomagnesemia in patients with diabetes was 17.1% (95% CI: 13.3–21.7%), and hypermagnesemia was 4.1% (95% CI: 2.4–7.0%). Females were significantly overrepresented in the hypomagnesemia group, while the hypermagnesemia group showed a higher prevalence of hypertension, retinopathy, an increased albumin/creatinine ratio, chronic kidney disease (CKD), elevated creatinine levels, and a lower adjusted calcium concentration. The multinominal logistic regression exhibited that the female sex and higher serum-adjusted calcium were independent risk factors of hypomagnesemia. In contrast, the presence of hypertension, higher levels of albumin/creatinine ratio, and stage 5 CKD were independent risk factors of hypermagnesemia. Conclusions: Hypomagnesemia was common among patients with diabetes mellitus; however, hypermagnesemia was associated with microvascular complications.

PESTI GUARD: FARMER-FRIENDLY PESTICIDE ROBOT

Agriculture is the primary source of revenue for India's population, which accounts for nearly 60% of the country's total. Farmers work in their fields to cultivate various crops based on the environment and resources available. Farmers must use large quantities of pesticides to increase food production in order to meet such high food demand for such a large population. Traditional manual pesticide spraying operations is full of direct exposure to the pesticide liquid work environment, great harm to human body and when this pesticide may come into contact with the farmer during spraying, which may trigger skin cancer and asthma illnesses. Increased pesticide spraying can impact consumer health as it enters the food chain. The widespread use of pesticides in agriculture presents significant health risks to both farmers and consumers. The pesticide spraying robot is equipped with state-of-the-art sensors to record real-time temperature and humidity levels, providing crucial environmental data for optimized pesticide application. Farmers can remotely control the robot using a user-friendly interface, instructing it to perform specific spraying tasks while maintaining a safe distance from potentially hazardous chemicals. To address this challenge, this paper proposes a Pesticide Spraying Robot designed to minimize human contact during operation while prioritizing human health through advanced environmental monitoring and remote-control capabilities.

Vitamin D in kidney: a two-edged sword?

A wide variety of both calcium-dependent and calcium-non-dependent actions are attributed to the vitamin and hormone vitamin D. One of the most vital components of the human body, vitamin D is essential to both health and illness. It is a member of the fat-soluble secosteroid family, which is obtained from diets or direct sun exposure, which is what turns 7-hydroxycholesterol into the precursor of vitamin D. Bio-activation is an alternate phase that provides an active version of vitamin D that contributes to several notable processes like detoxification, fertility, glucose regulation, bone remodelling, and calcium regulation. Numerous research investigations examine the discernible function of vitamin D in kidney illness. The basic physiological and pathological roles of vitamin D in the kidneys of both diabetics and non-diabetics were examined in this study. In this study we analyzed a basic physiological and pathological roles of Vitamin D in kidney in terms of diabetic and non-diabetic proteinuric kidney diseases. Further research in this field is of high importance.

The impact of the integration of digital platforms and active teaching strategies (Kahoot!) on the performance of Brazilian medical course students in the discipline of histology.

Teaching human histology is part of understanding the tissues of the human body and, therefore, it is part of the training curriculum of all health courses. The increase in technologies and active teaching methodologies has a positive impact on student learning, as it reduces the challenges present in the subject. Therefore, this work aimed to compare the performance of students in the histology discipline, when compared to traditional teaching methodology and its association with game-based learning and a basic histology teaching platform. Three classes of the medical course were selected between the years 2022 and 2023, each of which was separated into a group. One group did not have access to any platform, being called the Traditional Group (TG), a group that used Kahoot!, being called the Kahoot Group (KG), and a group that used the teaching platform, being called the Histoatlas Group (HG). Both KG and HG groups showed greater effectiveness in learning and improved performance, when compared to TG. These improvements in KG and HG were also highlighted as learning aids and easy to use. KG students performed better in the practical test when comparing groups. However, this difference was not observed in the students' averages. However, students pointed out the relevance of trying to improve the traditional teaching methodology. Therefore, this study points out that, even though the traditional teaching methodology is efficient in the student's teaching and learning process, there is a need on the part of students to make the subject more dynamic.

Construction of aptamer-based liquid crystal-aqueous sensing interface for the sensitive detection of dopamine

ABSTRACT Dopamine (DA) is an important neurotransmitter, playing significant roles in various psychological and physiological process of human body. However, the development of a simple, highly sensitive and cost-effective method for DA detection is still challenging. In this study, we report a simple, sensitive and label-free liquid crystal (LC)-based aptasensor for DA detection using a LC-aqueous interface with hexadecyltrimethylammonium bromide (CTAB) as a mediator. In the presence of DA, the specific DA-aptamer binding would weaken the electrostatic interaction between the aptamer and CTAB. This, in turn, promotes the assembly of CTAB at LC-aqueous interface and consequently induces a planar-to-homeotropic anchoring transition of LCs at the interface, which allows the detection of DA under polarized optical microscope (POM) with high sensitivity and specificity. By analysing the variation in dark pixel ratio in POM images of LC films, quantitative detection of DA can be achieved. The results show a linear detection range for DA from 10 pM to 1 μM with a detection limit as low as 2.51 pM (S/N = 3). Moreover, the feasibility of the LC-based aptasensor for DA detection in urine samples has been also validated.

A novel in-house built printed circuit board-ceria based electrochemical device for rapid detection of Epithelial Sodium Channel (ENaC), a hypertension biomarker

The Epithelial Sodium Channel (ENaC) is a protein that plays a role in the cellular intake of salt and is known as one of the biomarkers for hypertension in the human body. Higher concentrations of sodium lead to increased activity of the ENaC protein in sodium absorption. In this study, a self-made immunosensor was developed using a homemade Printed Circuit Board-Screen Printed Carbon Electrode (PCB-SPCE) modified with cerium oxide. Anti-ENaC was immobilized on the surface of PCB-SPCE/ceria at pH 7.4, relying on the interaction between carboxyl groups of anti-ENaC and cerium oxide to detect the ENaC protein. Additionally, a portable in-house potentiostat named “UnpadStat” was developed for integration with the immunosensor. UnpadStat features a 5-in. touch screen display to showcase measurement data. Cyclic voltammetry measurements using the redox system of K3[Fe(CN)6] were applied to bare PCB-SPCE, PCB-SPCE/Ceria, PCB-SPCE/Ceria/Anti-ENaC, and PCB-SPCE/Ceria/Anti-ENaC/ENaC. Measurement results indicated an increase in current after modification with cerium, reaching 73.805 µA compared to unmodified (63.256 µA). After anti-ENaC immobilization, there was a decrease in current to 64.456 µA, and upon binding with the ENaC protein, a further decrease in current occurred, corresponding to the concentration of the ENaC protein. The detection limit obtained in this study was 0.133 ng/mL, and the quantification limit was 0.444 ng/mL for the concentration range of ENaC protein from 0.375 ng/mL to 6.0 ng/mL. The integration of the PCB-based immunosensor and UnpadStat can be utilized to determine the levels of ENaC protein in non-hypertensive urine samples and hypertensive patients.

Multiscale Transfer Printing via Shape Memory Polymer with High Adhesion and Modulus Switchability.

Flexible electronics have gained significant attention as an innovative solution to meet the growing need for information collection from the human body and the environment. However, a critical challenge lies in the need for a transfer printing technique that can fabricate rigid and brittle devices on flexible organic substrates. Here, we develop a multiscale transfer printing technique using an ultraviolet-curable shape memory polymer (SMP) that serves as both the stamp and the receiver substrate. The SMP demonstrates exceptional mechanical performance with toughness at room temperature and remarkable flexibility near its glass transition temperature. The SMP material exhibits an impressive shape recovery ratio and remarkable adhesion switchability. We demonstrate robust transfer printing of diverse objects with different materials and morphologies and in situ transfer of multiscale metallic structures. In addition, the in situ fabricated transparent hyperthermia patches with embedded metal grids are demonstrated, offering potential application in the field of sensors, wearable devices, and electronic skin.

High Seebeck Coefficient Thermally Chargeable Supercapacitor with Synergistic Effect of Multichannel Ionogel Electrolyte and Ti3C2Tx MXene‐Based Composite Electrode

Thermally chargeable supercapacitors can collect low‐grade heat generated by the human body and convert it into electricity as a power supply unit for wearable electronics. However, the low Seebeck coefficient and heat‐to‐electricity conversion efficiency hinder further application. In this paper, we designed a high‐performance thermally chargeable supercapacitor device composed of ZnMn2O4@Ti3C2Tx MXene composites (ZMO@Ti3C2Tx MXene) electrode and UIO‐66 metal–organic framework doped multichannel polyvinylidene fluoridehexafluoro‐propylene ionogel electrolyte, which realized the thermoelectric conversion and electrical energy storage at the same time. This thermally chargeable supercapacitor device exhibited a high Seebeck coefficient of 55.4 mV K−1, thermal voltage of 243 mV, and outstanding heat‐to‐electricity conversion efficiency of up to 6.48% at the temperature difference of 4.4 K. In addition, this device showed excellent charge–discharge cycling stability at high‐temperature differences (3 K) and low‐temperature differences (1 K), respectively. Connecting two thermally chargeable supercapacitor units in series, the generated output voltage of 500 mV further confirmed the stability of devices. When a single device was worn on the arm, a thermal voltage of 208.3 mV was obtained indicating the possibility of application in wearable electronics.

A Study on a Health Impact Assessment and Healthcare Cost Calculation of Beijing–Tianjin–Hebei Residents under PM2.5 and O3 Pollution

A Study on a Health Impact Assessment and Healthcare Cost Calculation of Beijing–Tianjin–Hebei Residents under PM2.5 and O3 Pollution

A Self‐Powered Dual Ratchet Angle Sensing System for Digital Twins and Smart Healthcare

AbstractIn the swiftly progressing landscape of wearable electronics and the Internet of Things (IoTs), there is a burgeoning demand for devices that are lightweight, cost‐effective, and self‐powered. In this study, a self‐powered bidirectional knee joint motion monitoring system is introduced, leveraging a dual ratchet sensing (DRS) system fabricated using 3D printing technology. This approach offers substantial economic and portability benefits. The DRS system is engineered to harness the negative work generated from knee joint movements to power commercial electronic devices, obviating the need for additional metabolic energy from the human body. By synergizing the DRS with virtual reality technology, it becomes feasible to monitor knee joint movements in real‐time with remarkable accuracy, presenting a novel avenue for the integration of digital twin technology. Through the amalgamation of convolutional neural network machine learning algorithms with Bayesian optimization techniques, the DRS system can discern up to 97% of knee joint movements, paving the way for innovative applications in smart rehabilitation and healthcare.

The Insight Meditation Enhanced Stress, Depression, and Anxiety Reduction in Working Professionals Like Medical Representatives Determined by Using Serum Cortisol Level and Electroencephalogram: A Qualitative Study

The Indian pharmaceutical industry is one of the fastest-growing sectors of the Indian economy and ranks among the top 15 drug-manufacturing countries globally. Among the fastest-growing industries in the world, the pharmaceutical industry has increased over the years. A pharmaceutical company appoints a medical representative to make their products easily reach healthcare specialists and then be used by the general public. The medical representatives construct a well- built network with doctors, clinical specialists, medical practitioners, paramedical experts, Dietitians and nutritionists, paediatricians, gynaecologists, psychiatrists, etc. The problem of stress is widespread in every job description, and it may affect directly or indirectly in the form of health issues and low productivity; the demands of achieving targets induced among medical representatives. Much of this credit goes to Medical Sales Representatives or Pharmaceutical Sales Representatives (also known as MR, PSR) who are solely responsible for the promotion of products among Physicians, Hospitals, and pharmacies and are accountable for sales results. They act as the face of the Pharma company contributing to a better quality of life for customers, building goodwill, and increasing the company’s market share. Pharmaceutical sales representatives (PSR) play a large role in helping an industry in a rapidly changing environment to achieve a new corporate vision and normally these medical representatives are in the highest stress position. Most pharmaceutical sales representatives experience significant job stress and unhappiness levels. Stress, depression, and anxiety are the most common psychiatric disorders all over the world. One of India's oldest meditation methods, vipassana also known as Insight meditation, helps a person become aware of things as they are. The goal of the Vipassana method, which aids in self-change via self-observation, is to finally arrive at a condition of inner and exterior peace and mental equilibrium. It is a process of "Self- exploration" to the normal base of the brain and body that removes mental smog and results in a healthier personality filled with love and empathy. Electroencephalography (EEG) is an efficient modality that helps to acquire brain signals corresponding to various states from the scalp surface area. These signals are generally categorized as delta, theta, alpha, beta, and gamma based on signal frequencies ranging from 0.1 Hz to more than 100 Hz. This paper primarily focuses on EEG signals and their characterization for various states of the human body. Beta wave induced the production of cortisol. Also, the beta wave is necessary for our everyday lives, such as thought, communicating, arguing, and problem-solving, during conscious mental work. This Beta wave can also lead to tension, anxiety, and restlessness as they enable us to think properly. Persistently elevated cortisol concentrations are associated with metabolic disorders. Cortisol is a vital hormone sometimes known as the stress hormone in humans, as it is involved in the body's natural response to physical or emotional stress. cortisol has effects on memory, mood, and neurological diseases. Cortisol is best known for mediating the body’s reaction to stress. This study aims to review the existing research and challenges of serum cortisol levels (cortisol is an indicator of stress) and EEG signals with the Insight meditation effect.

Effects of trimline cut in ankle foot orthosis: An experimental and finite element analysis

An Ankle-foot orthosis (AFO) is a structure that spans from the lower leg to the foot and controls ankle joint movement. It prevents or assists the human body's lower leg and foot in replicating a normal human gait. The purpose of AFO is to achieve a stable gait by controlling the musculoskeletal system adequately. The mechanical features of AFO stiffness will play an essential role in helping gait, if this stiffness is not matching to the patient's conditions, gait will decrease and knee joint motion will be compromised. In general, trimline cut were introduced in the AFO medial and lateral side to control the stiffness of orthosis. However, this will result in stress concentration in the ankle region and lead to failure of AFO. In this study first evaluate the effects of trimline cut in AFOs using finite element analysis with three geometrical shapes like circle, elliptical and slot were introduced on the dorsal side. The stress concentration and stiffness of AFOs in the ankle region were computed. The stiffness of the basic model and trimline cut AFO model were compared and found elliptical trimline cut model is optimum one. The experimental analysis was performed using 3D Printed AFOs and calculated stiffness. It was observed that finite element analysis results, stress concentration of trimline cut models were reduced maximum of 2 % with basic model (without trimline cut)., whereas, in the experimental study of 3D-Printed AFO of trimline cut model, stiffness were reduced around 16 % compared with basic models. This study clearly indicates that, geometrical shapes trimline cut influencing on stiffness of AFOs. It will give the insight's for orthosis designers to make custom design of AFOs in particularly foot drop conditions to optimized the stiffness of orthosis.

Hereditary human diseases with skeletal pathology – molecular pathogenesis and clinical characteristics

Differential diagnosis of hereditary diseases is challenging due to similar clinical manifestations, diversity of nosologies, wide clinical variability and genetic heterogeneity, severity, rare frequency and complex molecular etiology. In some of them, connective tissue as a whole is involved in the pathological process, affecting almost all organs and systems of the human body, and in particular bone tissue, disrupting its remodeling and microarchitecture. The defect can occur at any of the stages of remodeling: during the initiation process, during bone resorption, osteoblast differentiation, osteoid mineralization, etc. Violation of the microarchitecture of bone tissue is accompanied by the development of low-traumatic fractures and deformities, early osteoarthritis, the formation of contractures and chronic pain syndrome. Genetic studies of patients have expanded knowledge about the molecular signaling pathways that coordinate bone development and metabolism, the nature of disease inheritance, clinical features, and specific bone biomarkers. This article provides an overview of key cellular mechanisms, features of diagnosis and treatment of hereditary human diseases that affect the condition of bone tissue and skeleton: osteogenesis imperfecta, EhlersDanlos syndrome, Marfan syndrome, juvenile osteoporosis, hypophosphatasia, osteopetrosis, progressive diaphyseal dysplasia, mucopolysaccharidoses, achondroplasia, multiple hereditary exostoses. Doctors of different specialties can encounter these diseases, and making a correct diagnosis will make it possible to determine the correct algorithm for patient management and begin timely treatment, the ability to prevent the development of severe complications, improve the quality of life of patients, restore maximum working capacity and reduce the percentage of disability.

Design and development of shape memory polyurethane coated fabric with excellent memory performance

AbstractIn this study, we investigate the fabrication of ambient room temperature responsive shape memory polyurethane (SMPU)‐coated fabric using a simple dip coating method, aiming to enhance the shape memory response of smart textiles. Two SMPU were synthesized using different chain extender 1,4 butanediol (BDO‐SMPU) and polyethylenimine (PEI‐SMPU). Critical parameters such as SMPU concentration, dwell time, and coating solution temperature are optimized to ensure exceptional shape memory performance while also maintaining the fabric's handle, comfort, and durability. We determined that PEI‐SMPU/Cotton fabric exhibits superior memory performance, with a 90.2% shape recovery at a 1% PEI‐SMPU concentration. Conversely, BDO‐SMPU fabric demonstrated a 75.8% shape recovery under different conditions (10% SMPU concentration, 50°C solution temperature). Furthermore, shape recovery is influenced by programming temperature and load; at 35°C and 10 N load, PEI‐SMPU/Cotton significantly outperforms BDO‐SMPU/Cotton, showing about 20% improvement in shape recovery. This study indicates that modifying SMPU from BDO‐SMPU to PEI‐SMPU can notably enhance shape recovery performance at 35°C, marking a significant advancement in human body temperature responsive smart fabric technology.

Numerical study on the effect of temperature rise of humeral bone nails in magnetic resonance imaging based on the finite-element method.

Humeral fracture is a common long bone fracture in orthopedic clinical diagnosis and treatment. To investigate the local temperature increase owing to changes in the specific absorption ratio (SAR) of the human body caused by humeral bone nails during magnetic resonance imaging (MRI). A refined geometric model of the upper body was constructed via data segmentation and post-processing using the digital human image dataset. Finally, the geometric model was imported into COMSOL, a 3-T magnetic resonance coil was built, and the operating frequency (128 MHz) was set to analyze the SAR of the bone-nail pair and temperature changes. The analysis of the changes after bone-nail implantation under different tissue conditions revealed that the SAR and temperature after implantation and fixation were three times higher than those before, and the areas with abrupt changes in SAR and temperature were primarily concentrated in the bone-nail area. In MRI, metal implants can cause local elevation of the SAR near the implant in the human body, resulting in a temperature increase around the implant. Consequently, long-term scanning can damage the human body.

Light-Adaptive Human Body Key Point Detection Algorithm Based on Multi-Source Information Fusion

The identification of key points in the human body is vital for sports rehabilitation, medical diagnosis, human–computer interaction, and related fields. Currently, depth cameras provide more precise depth information on these crucial points. However, human motion can lead to variations in the positions of these key points. While the Mediapipe algorithm demonstrates effective anti-shake capabilities for these points, its accuracy can be easily affected by changes in lighting conditions. To address these challenges, this study proposes an illumination-adaptive algorithm for detecting human key points through the fusion of multi-source information. By integrating key point data from the depth camera and Mediapipe, an illumination change model is established to simulate environmental lighting variations. Subsequently, the fitting function of the relationship between lighting conditions and adaptive weights is solved to achieve lighting adaptation for human key point detection. Experimental verification and similarity analysis with benchmark data yielded R2 results of 0.96 and 0.93, and cosine similarity results of 0.92 and 0.90. With a threshold range of 8, the joint accuracy rates for the two rehabilitation actions were found to be 89% and 88%. The experimental results demonstrate the stability of the proposed method in detecting key points in the human body under changing illumination conditions, its anti-shake ability for human movement, and its high detection accuracy. This method shows promise for applications in human–computer interaction, sports rehabilitation, and virtual reality.

Synchronous Switching Strategy to Enhance the Real-Time Powering and Charging Performance of Triboelectric Nanogenerator.

Triboelectric nanogenerators (TENGs) are of great significance as sustainable power sources that harvest energy from the human body and environment. Nevertheless, due to TENG's impedance-dependent output voltage characteristics, in traditional strategy (TS), real-timely powering a sensor with TENG has a poor sensing on/off ratio (or response), and directly charging a capacitor with TENG shows a low charging efficiency. This degraded real-time powering and charging performance of TENG compared to a commercial constant voltage source has been a huge challenge of the TENG field for a long time. Here, we propose a synchronous switching strategy (SSS) for TENG to real-timely power sensors or charge capacitors without degrading its performance. Compared with TS, this new strategy enables sensors to have 5-7 times sensing on/off ratio enhancement when using TENG as a power source, reaching the powering ability of a commercial constant voltage source, it makes the powering performance of TENG stable under different driving frequency, improving the powering robustness of TENG. In addition, compared with TS, SSS can also enhance the charging efficiency of TENG in every charging cycle by up to 2.4 times when charging capacitors. This work contributes to real-timely powering or charging the distributed, mobile and wireless electronics using TENG. This article is protected by copyright. All rights reserved.

Evaluation of adults' acumen exerted from cerebral stress with quantified anthropometric indices using bioelectrical impedance analysis

This work considers cerebral stress-struck adults to assess their ‘acumen’ with four quantified anthropometric indices like cell membrane capacitance (CM), total body impedance (TBI), total body fat (TBF), and total body water (TBW). Primarily, a bio-impedance model of the epithelial tissue structure of human body has been presented and anatomized considering mental stress driven hormonal changes happen inside human body to propose several preambles. Secondly, an intelligence quotient (IQ) test designed to create ‘racing’ thoughts has been conducted right before measuring indices using bioelectrical impedance spectroscopy (BIS) method. Finally, juxtaposed investigations to vindicate the correlation between bio-impedance model-implied preambles and the variance of the four quantified indices were made by comparing them to the IQ test outcomes. This article reports, an effectively innervated nervous system having homeostasis endocrine human anatomy with CM, TBI, TBF, and TBW, in the range of 1.0–2.1 nF, 470–550 Ω, 7.9–20%, and 56.8–80%, respectively for the subjects whose acumen is classified as “Class-A", while “Class-B" subjects are also presented with other distinct quantified indices. The outcomes of this work are expected to be a paradigm in the digital judgment of adults' psychological engagement to any tasks.

High Tartronic Acid Content Germplasms Screening of Cucumber and Its Response to Exogenous Agents

Tartronic acid is known for its potential to inhibit sugar-to-lipid conversion in the human body, leading to weight loss and fat reduction. This compound is predominantly found in cucumbers and other cucurbit crops. Therefore, cultivating cucumbers with high tartronic acid content holds significant health implications. In this study, we assessed the tartronic acid content in 52 cucumber germplasms with favorable overall traits and identified 8 cucumber germplasms with elevated tartronic acid levels. Our investigation into factors influencing cucumber tartronic acid revealed a decrease in content with fruit development from the day of flowering. Furthermore, tartronic acid content was higher in early-harvested fruits compared to late-harvested ones, with the rear part of the fruit exhibiting significantly higher content than other parts. Foliar spraying of microbial agents increased tartronic acid content by 84.4%. This study provides valuable resources for breeding high tartronic acid cucumbers and offers practical insights for optimizing cucumber production practices.

Heel-to-toe drop effects on biomechanical and muscle synergy responses during uphill walking

Uphill walking is a common task encountered in daily life, with steeper inclines potentially imposing greater biomechanical and neuromuscular demands on the human body. The heel-to-toe drop (HTD) in footwear may influence the biomechanical and neuromuscular pattern of uphill walking; but the impact remains unclear. Adjustments in HTD can modulate biomechanical and neuromuscular patterns, mitigating the demands and optimizing the body’s response to different inclinations. We hypothesize that adjustments in HTD can modulate biomechanical and neuromuscular patterns, mitigating the demands and optimizing the body’s response to different inclinations. Nineteen healthy men walked on an adjustable slope walkway, with varied inclinations (6°, 12°, 20°) and HTD shoes (10mm, 25mm, 40 mm), while the marker positions, ground reaction forces and electromyography data were collected. Our study reveals that gait temporo-spatial parameters are predominantly affected by inclination over HTD. Inclination has a more pronounced effect on kinematic variables, while both inclination and HTD significantly modulate kinetic and muscle synergy parameters. This study demonstrates that an increase in the inclination leads to changes in biomechanical and neuromuscular responses during uphill walking and the adjustment of HTD can modulate these responses during uphill walking. However, the present study suggests that an increased HTD may lead to elevated loads on the knee joint and these adverse effects need more attention.