Herpes Simplex Type 2 Viruses (HSV-2) are one of the most common viral sexually transmitted diseases worldwide, which are now a significant health concern. This study aim at prevalence of herpes simplex virus type 2 IgG and IgM and the associated risk factors among female students of Natural and Applied Science, Nasarawa State University, Keffi. Blood samples were collected from 150 female students of Natural and Applied Science. The socio-demographic information of the participants was collected by using a structured questionnaire. The sera samples were screened for IgGand IgM using NADALR HSV-2 IgG/IgM Rapid Antibody Test Cassette (Bulgarian Company for Biotechnology). The overall prevalence of HSV-2 IgG/IgM was 3.3% and 2.6%. The occurrence HSV-2 IgG in relation to the Age was high among age 26-30 years (100 %) and least among 21-25 years (2.0 %). The occurrence of HSV-2 IgM in relation to the Age was high among age 16-20 years (2.9 %) and least among was 21-25 years (2.0 %). the total prevalence of co-occurrence of HSV-2 IgG/IgM was 1.3%. the prevalence of In relation to knowledge of HSV-2 IgG and IgM in relation knowledge of HSV-2 infection was highest with no knowledge of HSV-2 recorded the IgG 4.5% and 3.4% with IgM. In relation to history of STI participants with history of STI recorded highest in IgG (23.5 %) and IgM (2.7 %). In relation to sharing sanitary facilities participants that share sanitary facilities recorded highest IgG 5.7% and IgM those that don’t share sanitary recorded highest 3.0 %. The prevalence of the HSV-2 infections was high among female students of Natural and Applied Science.