HPV Detection Rate Research Articles

Human Papillomaviruses Associated with Epidermodysplasia Verruciformis in Non-Melanoma Skin Cancers: Guilty or Innocent?

Abbreviations: AK, actinic keratoses; EV, epidermodysplasia verruciformis; HPV, human papillomavirus; ISH, in situ hybridization; NMSC, non-melanoma skin cancers; OTR, organ transplant recipients; SCC, squamous cell carcinomas; UVR, ultraviolet radiation

HPV oligonucleotide microarray-based detection of HPV genotypes in cervical neoplastic lesions

Background In this study we examined the use of a new-human papillomavirus (HPV) detection method, the HPV oligonucleotide microarray system (Biomedlab Co., Korea), which we compared with the well-established HPV DNA detection system (Hybrid Capture II; HC-II, Digene Co.). This new method prompted us to develop a new HPV genotyping technique, using the oligonucleotide microarray, to detect the generic and type-specific sequence of HPV types. In particular, we undertook the evaluation of the clinical efficacy of the HPV oligonucleotide microarray for detecting HPV in cervical neoplastic lesions. Methods One hundred forty patients were involved and classified into three groups according to their histopathologic diagnoses: Group I (nonspecific chronic cervicitis; n = 61), Group II (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia; n = 39), and Group III (high-grade SIL; moderate, severe dysplasia and in situ carcinoma; n = 40). Cytological diagnoses were based on the Bethesda System and cervical samples were analyzed by the two methods. The HPV oligonucleotide microarray detected 15 types of high-risk HPV (HPV-16/-18/-31/-33/-35/-39/-45/-51/-52/-56/-58/-59/-66/-68/-69) and 7 types of low-risk HPV (HPV-6/-11/-34/-40/-42/-43/-44). Results In 105 of the 140 cervical samples (75%), HPV DNAs were examined using the HC-II method. HPV detection rates using the HPV microarray agreed with those of HC-II. One HC-II-positive, but HPV microarray-negative, case occurred in the low-grade SIL (Group II) and was later confirmed negative for HPV. The other HPV microarray-positive but HC-II-negative case was found to be HPV-18 by PCR. Low-risk types of HPV were detected in 3 of 39 low-grade SIL cases (Group II) using the HPV microarray. HPV-16 was the most frequent type (32.1%) in all specimens tested, and was significantly more frequent in low-grade or high-grade intraepithelial lesions (Groups II or III) than in normal controls (Group I) ( P < 0.05). HPV-58 was the second most common type (17.5%) in Group III. The HPV microarray was found to have advantages in terms of identifying the HPV genotypes and cases of multiple HPV infection. Double HPV infections were detected in 12 cases and triple HPV infections in 7 cases. Two cases were positive for four types of HPV (HPV-16/18/33/35, HPV-16/18/58/68). The sensitivity of HPV testing (HC-II; 94.9%, HPV microarray; 93.7%) for identifying patients with squamous intraepithelial lesion was significantly better than the sensitivity of cytology (77.1%, P < 0.05). On using multiple logistic regression analysis to estimate the relative risk of SIL versus HPV type, HPV-16-positive cases were found to have a 7.5-fold risk of SIL (95% CI = 3.28–16.51; P < 0.01). HPV-33 and HPV-58 were found to be significantly related to high-grade SILs ( P < 0.01) ☆ This work was supported by Grant FG-4-12 of 21C Frontier Functional Human Genome Project from Ministry of Science and Technology of Korea. . Conclusions Our results suggest that the HPV oligonucleotide microarray is highly comparable to HC-II for detecting HPV in cervical specimens. The HPV oligonucleotide microarray provides useful information on viral genotype and multiple HPV infections in HPV-related cervical lesions. Genetic information on HPV in cervical specimens might be a particular benefit of the new procedure in the management of cervical neoplastic lesions

Improvement of hpv detection rate on cytological smears by pcr in situ hybridization

International Journal of Gynecology & ObstetricsVolume 70, Issue S3 p. C20-C20 Free communication Cervical carcinoma: Surgery and prognostic factors Improvement of hpv detection rate on cytological smears by pcr in situ hybridization Y.H. Xiao, Y.H. XiaoSearch for more papers by this authorS. Sato, S. SatoSearch for more papers by this authorY. Yokoyama, Y. YokoyamaSearch for more papers by this authorY. Saito, Y. SaitoSearch for more papers by this author Y.H. Xiao, Y.H. XiaoSearch for more papers by this authorS. Sato, S. SatoSearch for more papers by this authorY. Yokoyama, Y. YokoyamaSearch for more papers by this authorY. Saito, Y. SaitoSearch for more papers by this author First published: 10 December 2003 https://doi.org/10.1016/S0020-7292(00)81462-9AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume70, IssueS32000Pages C20-C20 RelatedInformation

Human papillomavirus deoxyribonucleic acid in cervix only detected in girls after coitus.

In a prospective cohort study on the prevalence of HPV in a group of female Swedish students, we obtained repeated cytological specimens during a period of 2 years, for cytological diagnosis and PCR detection of HPV. The group comprised 98 girls, sampled between 15 and 17 years, and 82 of them completed the study. The girls were also followed every sixth month by a structured face-to-face interview regarding sexuality. Only the sexually experienced girls harboured HPV-DNA in the cervix with a cumulative prevalence of 37% and HPV16 was the most common type encountered. Almost half of the infected girls were lacking any clinical or subclinical signs of HPV infection. There was a positive correlation between the presence of HPV and the number of coital partners. The time period since coitarche also had importance for the HPV detection rate.

Longitudinal Study of the Effects of Pregnancy and Other Factors on Detection of HPV

Objective: An epidemiologic study of 108 pregnant and 192 nonpregnant women was carried out to determine the effects of pregnancy and other factors on the detection of HPV infection, since both or larger numbers of pregnancies and HPV infection are known to be risk factors for cervical cancer. Patients and methods: Study participants were followed up at 3-months intervals for a period of 15 months (1–6 months after delivery). At each visit, two cervical specimens were taken, one for routine cytology and a second for HPV DNA hybridization using Virapap/Viratype, and a 5-ml blood sample was taken and a self-administered questionnaire was completed. Results: In cervical specimens of the initial examination, HPV DNA was detected among 5.0% of pregnant and 5.2% of nonpregnant women, whereas HPV 16/18 was found in 80% of the HPV-positive specimens. Using multiple cervical specimens taken over time, 13.9% of the pregnant and 15.1% of the nonpregnant women tested positive for HPV DNA at least once. Adjusted for study group, age, and the number of available cervical specimens, ever detection of HPV infection (any type) was associated with more sexual partners and larger numbers of cigarettes smoked daily. An autoregressive generalized linear model was used to analyze the time-dependent multiple observations per study subject. Adjusting for age and trimester of pregnancy, the determinants of detecting high-risk HPV types (16/18 and 31/33/35) in the cervical specimen were an abnormal Pap smear, a positive HPV result in a preceding specimen, more than six sexual partners in the lifetime, and currently smoking more than 20 cigarettes per day with odds ratios of 10.9, 5.6, 3.2, and 2.7, respectively. Conclusion: Our data provide no evidence for a higher detection rate of HPV during pregnancy.

Analysis of the characteristics of human papilloma virus infection in 85 neoplasms of the respiratory system in adult patients: Xing L, Liu H, Si J. Luoyang Medical College Hospital, Luoyang 471003. Chin J Oncol 1994;16:424–427

Eighty five neoplastic tissues of respiratory system in Chinese adult patients were searched for HPV by multiple PCR and in situ hybridization. In 13 of the specimens examined, HPV DNA was identified (15.3%), including 6 of 36 cases of pharyngolaryngeal neoplasms (16.6%) and 7 of 49 cases of lung squamous cell carcinomas (14.2%). Among the 13 cases tumor specimen, most of them were positive for HPV6/11 types and a few were double positive for HPV 6/11 and 16 types. Many tumor cells positive for HPV DNA on in situ hybridization were morphologically similar to those koilocytes in genital condylomas. HPV infection in 85 neoplasms was characterized by the fact that the same genotypes of HPV were detected in upper and lower respiratory tract and that the dominant virus types detected were HPV 6/11 types. The detection rate of HPV was a little higher in the upper than that in the lower respiratory tract and the HPV DNA positive tumors had similar histological appearance. The results suggest that HPV infection in the neoplasms of the upper and the lower airways is related to each other with similar mode of transmission.

Prevalence of HPV in premalignant and malignant cervical lesions in Greenland and Denmark: PCR and in situ hybridization analysis on archival material

Archival formalin-fixed paraffin-embedded cervical specimens from 125 women in Greenland and 139 women in Denmark who had CIN I-III or cervical cancer diagnosed between 1983 and 1987 were analysed for human papillomavirus type 16 (HPV-16) by in situ hybridization and for HPV-16, 18, 31, 33, 35 and 45 by PCR. In situ hybridization analysis showed an HPV-16 prevalence of 17% (16/95) and 23% (24/105) in the premalignant lesions from Greenland and Denmark, respectively. The HPV-16 prevalence rate in the cancer specimens was 10% (3/30) in the samples from Greenland and 29% (10/34) in the Danish specimens. A total of 82 Greenlandic and 107 Danish specimens were beta-globin-positive by PCR reaction. HPV-16-specific PCR on these samples showed 63% (34/54) of the Greenlandic and 68% (50/74) of the Danish preinvasive lesions to be positive. The corresponding HPV-16-positive rates for the invasive cancers were 82% (23/28) for Greenland and 70% (23/33) for Denmark. This study of patient samples supports our previous population-based studies in which similar HPV-detection rates were found among random samples of women from Greenland and Denmark, although Greenland has a 4-5-fold higher cervical cancer incidence.

Demonstration of human papillomavirus DNA by nucleic acid in situ hybridization in paired histologically abnormal cervical biopsies obtained at the same patient visit.

Fifty-four pairs of cervical biopsies ranging from minimal dysplasia to severe dysplasia were studied for the presence of human papillomavirus DNA by in situ hybridization. Two assays were performed on each biopsy. A 16 hour hybridization was used in one assay, while a 40 hour hybridization was utilized in the second assay. Increasing the hybridization time to 40 hours did not significantly increase the detection rate of HPV compared to the rate found using the 16 hour hybridization. Also, no difference in the detection rate of HPV was found by using one biopsy of the pair over the other biopsy of the pair. However, the performance of a single in situ assay on only one biopsy from each patient significantly underestimated the true prevalence of HPV. A single assay only detected 21/33 (64%) patients with HPV. Implications of multiple testing of all histologically abnormal biopsies is discussed in relation to prospective follow-up studies determining the usefulness of HPV typing in patient management.

Colposcopic Survey of Papanicolaou Test-Negative Cases With Hyperkeratosis or Parakeratosis

A colposcopic survey was performed in 269 consecutive women with negative cytology showing hyperkeratosis or parakeratosis. A colposcopy-guided biopsy specimen of cervical abnormalities was taken in 88 cases, and human papillomavirus infection (HPV) was detected histologically in 25 cases. No cervical intraepithelial neoplasia (CIN) was detected. The detection rate of HPV was not significantly different from that observed in a consecutive series of 1073 Papanicolaou test-negative subjects self-referred for colposcopy. Colposcopic screening of subjects showing hyperkeratosis or parakeratosis with otherwise negative smears is not recommended because it does not allow detection of cytologically false-negative CIN.