ObjectiveAnanus comosus (L.) is belonging to the family Bromeliaceae used to cure various diseases. The present study is focus to evaluate the HPTLC fingerprinting analysis and phytochemical analysis of Ananus comosus peel. MethodsHPTLC fingerprinting profiles was done by using Hamilton syringe and CAMAG LINOMAT 5 instrument. ResultsAmong all solvents, most of the secondary metabolites such as alkaloid, flavanoid, phenol, tannins, steroids etc were present in ethanolic extract of Ananus comosus peel and the yield of plant extract in different solvents were analyzed, from which ethanolic extract gave 2.40g/100g. The quantitative assay was also analyzed in flavanoid, alkaloid, phenols, carotenoid and lycopene. HPTLC fingerprinting profile confirms the presence of phenols, flavanoid and alkaloid. ConclusionFrom the above results ethanolic extract of Ananus comosus peel can be used as therapeutic agents to treat various disorders caused by free radical and chemical substances due to presence of its secondary metabolites.