Household Dogs Research Articles

Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China

BackgroundThere are only limited number of reports on molecular epidemiology of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in China. This study was conducted to assess the infection rates, genetic identity, and public health potential of these parasites in dogs and cats in Guangdong, China.MethodsPCR and sequence analyses were used to identify and genotype Cryptosporidium spp. and G. duodenalis in fecal samples from 641 dogs and 418 cats in Guangdong. Chi-square test and odds ratio analysis were used to compare the occurrence rates of these pathogens and identify risk factors for infection.ResultsThe overall infection rates of Cryptosporidium spp. and G. duodenalis were 6.9% (44/641) and 9.4% (60/641) in dogs, and 6.2% (26/418) and 3.6% (15/418) in cats. Purebred cats (12.4%; χ2 = 5.110, OR = 2.8, P = 0.024) and dogs (10.8%; χ2 = 5.597, OR = 4.8, P = 0.018) were more likely to be infected by Cryptosporidium spp. and G. duodenalis, respectively. Dogs (12.0%; χ2 = 7.589, OR = 2.6, P = 0.006) and cats (13.6%; χ2 = 8.235, OR = 3.5, P = 0.004) under 6 months had significantly higher infection rates of Cryptosporidium spp. than older animals. Household (13.9%; χ2 = 10.279, OR = 2.6, P = 0.008) and pet shop dogs (11.0%; χ2 = 7.182, OR = 2.0, P = 0.048) had higher occurrence of Cryptosporidium spp., as was the case for G. duodenalis occurrence in experimental dogs (13.4%; χ2 = 9.223, OR = 1.9, P = 0.017). Cryptosporidium canis (n = 42), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in dogs, while C. felis (n = 21), C. parvum (n = 3), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in cats. In contrast, the canine-specific assemblages C (n = 27) and D (n = 26) and the feline-specific assemblage F (n = 14) were almost exclusively the only genotypes of G. duodenalis in dogs and cats, respectively. There was no significant difference in infection rates of Cryptosporidium spp. and G. duodenalis between diarrheal and non-diarrheal pets.ConclusionsWhile domestic pets in Guangdong are infected with zoonotic Cryptosporidium species, they are mainly infected with host-specific G. duodenalis genotypes. Risk factors for infections differ between Cryptosporidium spp. and G. duodenalis and between dogs and cats.

Host factors associated with Giardia duodenalis infection in dogs across multiple diagnostic tests

BackgroundThe aim of this study was to assess potential associations between Giardia duodenalis infection in dogs, as determined by three diagnostic tests, and dog’s group of origin, fecal consistency, age, sex, neuter status, and co-infections with other gastrointestinal parasites.MethodsFecal samples from 1291 dogs from four groups (household, shelter, hunting and clinical dogs) were tested with qPCR, rapid enzyme immunochromatographic assay (IDEXX SNAP®Giardia), and direct immunofluorescence (DFA, Merifluor) for presence of G. duodenalis. Moreover, fecal samples were tested with centrifugation sedimentation flotation (CSF) coproscopical analysis for presence of gastrointestinal parasites. Associations were expressed as odds ratios (ORs).ResultsSeveral significant associations were found, of which a few were consistent for all three tests and Giardia positivity in general (positive with at least one of these tests). Dogs older than one year were significantly less likely to test positive for Giardia than younger dogs. Group-housed dogs, especially hunting dogs, were significantly more likely to test positive for Giardia compared to household and clinical dogs. A consistently significant association with Trichuris appeared to be driven by the high prevalence in hunting dogs. Although there was no significant association between loose stool and Giardia infection in the overall population, household dogs were significantly more likely to test Giardia-positive when having loose stool. Overall, Giardia-positive dogs with loose stool shed significantly more cysts, both determined semi-quantitatively with CSF and quantitatively by qPCR, than positive dogs with no loose stool. When other gastrointestinal parasites were present, significantly fewer cysts were detected with CSF, but this was not confirmed with qPCR.ConclusionGiardia is the most common gastrointestinal parasite in Dutch dogs, except for hunting dogs, in which Trichuris and strongyle-type eggs (hookworms) prevailed. Giardia infection was not significantly associated with loose stool, except for household dogs. Young dogs and group-housed dogs were significantly more often Giardia-positive. These associations were consistent across diagnostic tests. Young dogs, clinical dogs and dogs with loose stool shed Giardia cysts in the highest numbers. If another gastrointestinal parasite was present lower numbers of cysts were observed by microscope (CSF), but not with a molecular method (qPCR).

Forgotten, But Not Lost-Alloparental Behavior and Pup-Adult Interactions in Companion Dogs.

Simple SummaryCompanion dogs are vastly popular animals; however, we know surprisingly little about their natural parental behaviors. Meanwhile, although wolves, dingoes, and, to an extent, even free-ranging dogs show several forms of alloparental behaviors, the parental care among companion dogs is thought to be solely provided by the mother. We circulated an international survey for dog breeders, asking them about the forms of alloparental behaviors they observed among their dogs, as well as further interactions between the puppies and other adult dogs at home. Our results show that allonursing and feeding of the pups by regurgitation is a widespread phenomenon among companion dogs. The behavior of young puppies regarding, for example, their reaction to other dogs’ barking was also influenced by the timing of their access to the other dogs at the breeder’s home. Based on the breeders’ observations, sexual status and age of the other dogs affected the way they interacted with the puppies, and also the way the puppies’ mother interacted with them. These results highlight the importance of dog–puppy interactions during the early weeks of life, an often neglected area compared to the well-known elements of puppy socialization with human beings.Socialization with humans is known to be a pivotal factor in the development of appropriate adult dog behavior, but the role and extent of dog–dog interactions in the first two months of life is rarely studied. Although various forms of alloparental behaviors are described in the case of wild-living canids, the social network of companion dogs around home-raised puppies is almost unknown. An international online survey of companion dog breeders was conducted, asking about the interactions of other dogs in the household with the puppies and the pups’ mother. Based on the observations of these breeders, our study showed an intricate network of interactions among adult dogs and puppies below the age of weaning. Alloparental behaviors (including suckling and feeding by regurgitation) were reportedly common. Independent of their sex, other household dogs mostly behaved in an amicable way with the puppies, and in the case of unseparated housing, the puppies reacted with lower fear to the barks of the others. Parousness, sexual status, and age of the adult dogs had an association with how interested the dogs were in interacting with the puppies, and also with how the mother reacted to the other dogs. Our study highlights the possible importance of dog–dog interactions during the early life of puppies in forming stable and low-stress interactions with other dogs later in life.

Risk factors of human cystic echinococcosis in Shiqu County Sichuan Province: a case-control study

To identify the main risk factors of human cystic echinococcosis in Shiqu County, Sichuan Province, so as to provide the reference for the formulation of echinococcosis control strategies in Shiqu County. During the period from November 2015 through June 2017, the patients with cystic echinococcosis (case group) and healthy controls (control group) were randomly sampled from Shiqu County as the study subjects. A questionnaire survey was conducted to capture the study subjects'age, gender, ethnicity, occupation, religion, lifestyle, education level, number of household dogs, bovine and sheep, and density of dog feces in the courtyard. The major risk factors of human cystic echinococcosis were identified using a logistic regression model. Univariate logistic regression analysis showed 7 risk factors with statistical significance between the case and control groups, and age, lifestyle, number of household bovine, number of household sheep, number of house-hold dogs, and the density of dog feces in the courtyard were included in the multivariate logistic regression model (OR = 1.026, 4.792, 1.067, 1.022, 1.709 and 1.095, respectively). High age, pastoral nomadic lifestyle, high number of house-hold bovine, high number of household sheep, high number of household dogs and high density of dog feces in the courtyard are strongly associated with the riks of human cystic echinococcosis in Shiqu County.

Physiological Responses of Dogs to Different Housing Systems

Background: The hypothalamic-pituitary-adrenal (HPA) axis undergoes adaptations throughout housing system that might contribute to the avoidance of adverse effects of welfare status in dogs housed in a shelter. Nevertheless, the influence of housing systems and stabling time on glucose and PCV changes is little known. The purpose of the present study was to evaluate the patterns of cortisol, glucose and PCV in dogs housed in a kennel and normal environments, evaluating the differences between housing systems, by taking account the different stabling time and sex. Materials, Methods & Results: The study comprised 98 cross-breed dogs, aged 4 ± 1.5 years, lodged in a kennel (observational group I: N= 61, 29 females and 27 males), in paired household dogs (control group II: N= 25, 13 females and 12 males) and in unpaired household dogs (control group III: N= 12, 6 females and 6 males). Females of both groups were spayed. The subjects were studied on the basis of different stabling times, ranged among <1 year, 2 years and 4 years, and different sex.Discussion: This observational study showed that kennelled males lodged for 2 (P < 0.01) and 4 (P < 0.001) years showed lower cortisol concentrations than males lodged <1 year, males lodged for <1 year (P < 0.001) showed higher cortisol concentrations than females; males lodged for 4 year showed lower cortisol concentrations (P < 0.01) than females. Kennelled females lodged for 4 year showed higher PCV values (P < 0.001) than females lodged for <1 year. Paired and unpaired household females and males lodged for 4 years showed lower cortisol concentrations (P < 0.01) than 2 years and <1 year. Paired and unpaired household females and males lodged for short-, medium- and long-term times showed higher glucose concentrations (P < 0.001) than kennelled dogs. This study showed significant changes of circulating cortisol, glucose and PCV values inter- and intra-groups, according to different housing systems, stabling time and sex. The magnitude of cortisol decreases after 2 and 4 years of stabling time in kennelled male dogs could suggest a stimulus-response relationship, probably due to adaptive responses. Interestingly enough in this observational study was that the exact consummatory event could be represented by the long stabling time itself, observed only in males. Moreover, the significant differences of cortisol concentrations between males and females in group I after a stabling time of <1 and 4 years confirm the wide variability of HPA activity independent of the different sex of dogs. The physiological higher glucose concentrations in household dogs than kennel dogs showed that the normal home environment offers probably more rich stimuli than kennel, independent of stabling times and sex. It is possible to suppose that the housing system of dogs also results in significant rise of organ reserve and adaptability, presumably due at early greatest in functional changes in the catecholaminergic system and related increased hepatic glycogenolysis and gluconeogenesis. Concerning the PCV changes, data obtained confirmed the disagreement among authors about sex differences in the circulating mass of erythrocytes according to different age and sex. In conclusion, this is an observational study on the adaptive responses of circulating cortisol, glucose and PCV patterns to different housing systems in both kennel and household, leading to the question of physiological relevance concerning the effects of different stabling, according to the quality of dogs’ life.

VOC pattern recognition of lung cancer: a comparative evaluation of different dog- and eNose-based strategies using different sampling materials

Background: It has been reported that canine scent tests offer the possibility to screen for cancer. Assuming that breath samples can be collected with carrier materials, we tested the practicability of different carrier materials to be presented to dogs and validated and compared results with an electronic nose (eNose). Moreover, we hypothesized that cancer detection ability of dogs differs according to their working experience.Methods: In a methodological approach, two dog teams participated, one using experienced working dogs and the other ordinary household dogs to find the most qualified dogs and training method. To find best carrier material for breath sampling we compared charcoal containing glass tubes and fleece masks. In a second validating part, experienced working dogs were trained with improved training strategies. For breath sampling, two different, previously successfully tested fleece-based carrier materials were used: one was used with the dog team and both materials were compared with eNose.Results: In the methodological approach, it turned out that the charcoal-based sampling strategy qualified not sufficiently for VOC-detection. Moreover, we could determine that using experienced working dogs provided several advantages. Overall results of dogs in the validating part regarding specificity were 83%, regarding sensitivity 56%, but with great variability among dogs. Using eNose for breath analysis collected with both fleece carrier materials, specificity was 97% and sensitivity 89–100%.Conclusion: Our data confirmed that the diagnostic accuracy of dogs depended on the type of dog training and on the carrier materials. A comparison of breath samples analysis with an eNose achieved better results for both, sensitivity and specificity than for dogs. The use of fleece masks or fleeces in glass tubes as a sampling material can be recommended as successful VOC carriers, encouraging their use for clinical screenings.

Retrospective and Comparative Study of Giardia sp. Prevalence in Dogs, Cats, and Small Ruminants in Endemic Areas in Different Brazilian States

Background: Giardia, an intestinal parasite of asexual reproduction, is an important etiologic agent of diarrhea in animals and humans, transmitted by orofecal route. The disease caused by this agent, giardiasis, is endemic in the world and representing an important public health problem. The aim of the present study was do a retrospective study to determine the prevalence of Giardia sp. in fecal samples from dogs, cats, sheep and goats (small ruminants) evaluated at FMVZ Unesp, Botucatu, SP. In addition, this study presents a review of the literature on the prevalence of this protozoan in dogs, cats and small ruminants in different states of Brazil, according to the environmental of these animals.Materials, Methods & Results: During 2011 to 2017, 2,698 fecal samples of dogs, 359 fecal samples of cats and 320 fecal samples of goats and sheep were analyzed. A total of 18.9% of the dogs, 24.8% of the cats, and 6.6% of the goats and sheep were positive for Giardia sp. Only previous studies that used the zinc sulphate centrifugal flotation (Faust technique) were included for comparation of prevalence. The prevalence of Giardia sp. in samples of centrifugation-fluctuation in zinc sulfate was similar in dogs, higher in cats and lower for small ruminants when compared to previous investigations. 4.4% and 6.7% of the positive samples from dogs and cats, respectively, were associated with some co-infection. Co-infections by Cystoisospora spp., Ancylostoma spp. and/ or Toxocara spp. were the most common for dogs and cats.Discussion: This study reveals the presence of Giardia sp. in different animal species in an endemic área. The results are similar to the prevalence previous reported in dogs, and higher in cats using the same diagnostic technique (Faust technique). Co-infections by Cystoisospora spp., Ancylostoma spp. and/ or Toxocara spp. were the most common for dogs and cats, as well-known in previous studies. Investigations with household, shelter and stray dogs found a significantly lower occurrence of Giardia sp. in the group of household dogs. However, for cats these comparisons should made with caution. Despite having a owner, some of these cats have free access to the streets, so they might be more expose to the sources of infection. There are few previous reports of the presence of Giardia sp. in goats and sheep, which presented much higher prevalence when compared with this study. This variation in prevalance of Giardia sp. in small ruminants can occur due to differences inherent to each region in Brazil, as well as the presence of risck factors regarding animal age, type of raising of the animals, hygienic-sanitary and management conditions, and the presence of domestic animals inside sheep and goat installations, which can potentially favor the mechanical transmission of cysts our reservoirs of this parasite. Therefore, due to regional variations in this parasite prevalence, this information is more value in regional areas, reaffirming the importance of this kind of studies in brazilian states and cities. The intermittent elimination of cysts in feces, the low number of cysts in the samples and asymptomatic infections are relevant points to the diagnosis of giardiasis, making it a challenge. The technique of centrifugation-flotation technique in zinc sulfate gives the best results when compared to other techniques available for the diagnosis of Giardia infections, justifying the choice of this technique by this study. These evidences associated to molecular diagnoses are necessary to determine the real role of these animals in the epidemiology and zoonotic transmission, due to the close contact with humans. This may be considering as a start for further investigations of Giardia sp. at municipality of Botucatu.

Acquiring a Pet Dog: A Review of Factors Affecting the Decision-Making of Prospective Dog Owners.

Simple SummaryEach year, many people around the world get a pet dog. With so many different types and breeds of dogs available, and a variety of sources from which to obtain a dog, the process of getting a dog can be complex. The decisions involved in this process are likely influenced by a variety of human- and dog-related factors and this review explores the factors that appear to be the most important.Given the prevalence of pet dogs in households throughout the world, decisions regarding dog acquisition affect many people each year. Across the stages of dog acquisition there is potential for practices that may promote or compromise canine welfare. For instance, prospective owners may not fully understand the time, energy and financial commitment entailed in their decision to acquire a dog. Thus, it is pressing that stakeholders, including those working in the canine welfare sector, refine their ability to identify and respond to trends in the behavior of potential dog owners. The motivations, attitudes and behaviors of current and prospective dog owners is a small but growing area of interdisciplinary study. Yet, no synthesis of the evidence exists. To address this gap, this critical review collates data and insights from studies published by academic researchers and animal welfare charities. The most widely reported factors associated with acquisition behavior include: the dog’s physical appearance, behavior and health; social influences, such as trends in the popularity of certain breeds; demographic and socioeconomic factors; and the owner’s previous ownership experience. Overall, the research discussed in this paper highlights that complex interactions likely underpin the various factors that might influence prospective owners’ motivators and behaviors.

OCCURRENCE OF FILARID PARASITES IN HOUSEHOLD AND SHELTERED DOGS IN THE CITY OF JOINVILLE – SANTA CATARINA, BRAZIL

Abstract Parasites from the Filariidae family are among the nematodes that can infest dogs. Some may be apathogenic, such as Acanthocheilonema reconditum, and others such as Dirofilaria immitis are potentially zoonotic and may lead dogs to death. Thus, the aim of this study was to perform a survey on filarid infection in dogs with a fixed domicile and wandering dogs temporarily sheltered in the city of Joinville, SC, Brazil. Blood samples from 429 animals were collected from 2015 to 2017 and submitted to screening for microfilariae and/or D. immitis antigens. Our results revealed that twenty-four samples (5.6%) displayed A. reconditum microfilariae and three samples (0.7%) were positive for D. immitis, totaling 27 (6.3%) positive animals for filarid parasites. Additionally, A. reconditum was more prevalent in dogs from shelters, whereas the three D. immitis positive samples were from household dogs that displayed no clinical signs of the presence of parasites. The occurrence of these parasites in dogs from Joinville indicates the importance of differential diagnosis of microfilaria species for the correct clinical management of infected dogs. Our epidemiological information may help the medical and veterinary community of the region regarding clinical suspicion of canine and human heartworm disease, as well as guide preventive actions targeting public and animal health.

Pet-keeping in early life reduces the risk of allergy in a dose-dependent fashion.

ObjectivesSeveral studies have indicated that early pet keeping could protect the infant from later allergy development. Here, we investigate if there is a dose-dependent association between cat- and dog-keeping during the first year of life and subsequent allergy development.MethodsTwo cohorts were investigated: a cross-sectional questionnaire-based study of 7- to 8-year-old children (N = 1029) from Mölndal and Kiruna, and a birth-cohort of children from the Västra Götaland county clinically evaluated for asthma and allergy by paediatricians up to the age of 8–9 years (N = 249). The cross-sectional study asked validated questions on asthma and allergy that had been used in two previous studies of children from the same areas. In the birth-cohort study, a diagnosis of asthma and allergy was based on predefined clinical criteria, and laboratory evaluation included blood eosinophils, skin-prick tests and specific immunoglobulin E analyses. Information on pets during first year of life was collected retrospectively in the Cross-Sectional Cohort and prospectively in the Birth Cohort.ResultsA dose-response association was seen, with less allergic manifestations (any of asthma, allergic rhinoconjunctivitis, or eczema) with increasing number of household cats and dogs during the first year of life. In the Cross-Sectional Cohort, allergy ever decreased from 49% in those with no pets to zero in those with five or more pets (P-value for trend 0.038), and from 32% to zero for allergy last year (P-value for trend 0.006). The same pattern was seen in Birth Cohort. Sensitization to animals, as well as pollens, also decreased with increasing number of animals in the household.ConclusionThe prevalence of allergic disease in children aged 7–9 years is reduced in a dose-dependent fashion with the number of household pets living with the child during their first year of life, suggesting a “mini-farm” effect, whereby cats and dogs protect against allergy development.

Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines

BackgroundThe tropical climate of the Philippines and the high population of dogs, particularly in cities, favors the life-cycle of the brown dog tick, Rhipicephalus sanguineus (sensu lato), a vector of several canine tick-borne pathogens (TBPs) including zoonotic Rickettsia spp. Suspected cases of infections are commonly encountered in veterinary clinics, but the specific TBPs are rarely identified. Furthermore, infection with Rickettsia is not being clinically examined in dogs. In this study, the occurrence of TBPs in blood and ticks collected from household and impounded dogs in highly populated areas of the Philippines, Metro Manila, and the nearby province of Laguna, was examined.ResultsA total of 248 blood samples and 157 tick samples were subjected to PCR. First, samples were screened using primers for Anaplasma/Ehrlichia spp. and Babesia/Hepatozoon spp. Those that turned positive were further subjected to species-specific PCR. Rickettsia spp. were also detected through a nested PCR. Of the 248 blood samples, 56 (22.6%) were positive for Anaplasma/Ehrlichia spp., while 19 (7.6%) were positive for Babesia/Hepatozoon spp. Species-specific PCR revealed that 61 (23.4%) had a single TBP, with Ehrlichia canis being detected in 39 (15.7%) dogs, while 14 (5.6%) dogs were positive for different combinations of two to four TBPs. Rickettsia infection was detected in 6 (2.4%) dogs. In tick samples, 8 (3.2%) were positive for Ehrlichia/Anaplasma spp., while only 1 (0.63%) was positive for Babesia/Hepatozoon spp. As in the blood samples, E. canis was the most detected, being found in 5 (2%) samples. No tick samples tested positive for Rickettsia spp.ConclusionEhrlichia canis is the most common TBP affecting dogs in the Philippines. Co-infection with TBPs is quite common, hence testing for multiple TBPs is necessary. Through nested PCR, Rickettsia infection was detected in dogs, and to the authors’ knowledge, this study provides the first molecular evidence of Rickettsia infection in dogs in the Philippines.

Considering Dogs as Complementary Targets of Chagas Disease Control.

This review describes the role that dogs play in Latin American countries where Chagas disease is endemic. Multiple studies determined the high frequency with which canine populations are infected with Trypanosoma cruzi. The infection prevalence of dogs is greater than that of humans and the presence of infected dogs in households is associated with a higher risk of human infection. Dog infectiousness to triatomine vectors is several-fold higher than that of humans, thereby underscoring their major role in the domestic transmission of T. cruzi. Insecticide spraying of houses is in most cases efficacious but the lack of sustainability hinders this vector-focused strategy. Multi-pronged approaches have been adopted to improve control measures but dog intervention was never included. Experimental evaluation of systemic insecticides or deltamethrin-impregnated collars suggested that dog intervention leading to triatomine killing could curb domestic transmission of T. cruzi. Larger field studies are required to determine its applicability and efficacy. However, the implementation of dog intervention could complement other control measures currently in place, mostly in periods when vector spraying has been interrupted.

Comparing four diagnostic tests for Giardia duodenalis in dogs using latent class analysis

BackgroundTo accurately diagnose giardiosis in dogs, knowledge of diagnostic test characteristics and expected prevalence are required. The aim of this work was to estimate test characteristics (sensitivity and specificity) of four commonly used diagnostic tests for detection of Giardia duodenalis in dogs.MethodsFecal samples from 573 dogs originating from four populations (household dogs, shelter dogs, hunting dogs and clinical dogs) were examined with centrifugation sedimentation flotation (CSF) coproscopical analysis, direct immunofluorescence assay (DFA, Merifluor Cryptosporidium/Giardia®), a rapid enzyme immunochromatographic assay (IDEXX SNAP Giardia®) and qPCR (SSU rDNA) for presence of G. duodenalis. Bayesian latent class analysis was used to determine test performance characteristics and to estimate G. duodenalis prevalence of each of the four dog populations.ResultsAll tests were highly specific. IDEXX SNAP Giardia® showed the highest specificity (99.6%) and qPCR the lowest (85.6%). The sensitivities were much more variable, with qPCR showing the highest (97.0%) and CSF the lowest (48.2%) sensitivity. DFA was more sensitive than IDEXX SNAP Giardia®, but slightly less specific. Prevalences of G. duodenalis differed substantially between populations, with the hunting dogs showing the highest G. duodenalis prevalence (64.9%) and the household dogs the lowest (7.9%).ConclusionsThis study identifies qPCR as a valuable screening tool because of its high sensitivity, whereas methods using microscopy for cyst identification or cyst wall detection should be used in situations where high specificity is required. G. duodenalis is a prevalent gastro-intestinal parasite in Dutch dogs, especially in dogs living in groups (hunting and shelter dogs) and clinical dogs.

Campylobacter infection occurrence in canine population in Italy

Background: In Europe, Campylobacteriosis is the most frequently reported zoonotic disease in humans. Approximately 6% of human campylobacteriosis cases are due to contact with pets, particularly dogs, which may be reservoir of a wide range of Campylobacter species. These populations should therefore be regarded as potential sources of exposure for humans. Antimicrobial resistance of Campylobacter strains, especially multi-drug resistance isolates, are also increasing. This study aims at investigating the occurrence of Campylobacter infection and related antimicrobial resistance of isolates in a selected dog population in Italy. Methods & Materials: Four-hundred and thirty one samples from household dogs and 173 samples from sheltered dogs were tested for Campylobacter spp. The isolates were all investigated for phenotypic and genotypic characterization (PCR, PFGE, MLST, NGS), and for antimicrobial resistance using the micro dilution method. Results: Fourty-seven out of 431 samples of household dogs tested positive to Campylobacter spp., and 9 strains (2.1%) were typed (4 C. jejuni, 1 C. coli, 1 C. lari, 2 C. upsaliensis, 1 C. vulpis.). Thirty-five out of 173 samples of sheltered dogs investigated tested positive to Campylobacter spp., and 13 strains were typed (7.5%) (9 C. jejuni, 3 C. lari and 1 C. coli). Campylobacter isolates were resistant to ciprofloxacin (27,7%), Nalidixic Acid (27,7%), Tetracycline (33,3%), Streptomycin (11,1%), and Erytromicin (5,6%). Conclusion: C. jejuni represents the main cause of zoonoses in humans and, according to our study, is the main Campylobacter species isolated in Italian canine population. The consequence's in terms of public health and the possible preventive measures are discussed.

Canine seroprevalence to Orientia species in southern Chile: A cross-sectional survey on the Chiloé Island.

BackgroundScrub typhus is a potentially life-threatening vector-borne infection caused by Orientia species. It occurs mainly in the Asian-Pacific region, where it causes significant morbidity and mortality. Recently, an endemic focus of scrub typhus has been described in South America, on Chiloé Island in southern Chile. Dogs have been used as sentinel hosts to determine the presence and spatial distribution of various vector-borne infections. Their suitability to gain insight into human exposure to Orientia tsutsugamushi has been suggested in studies from Asia.MethodologyIn January 2016, we conducted a cross-sectional study, which included the two main cities on Chiloé Island. Canine blood samples were obtained in households, chosen by double stratified random sampling in urban and by convenience in rural locations. Specimens were tested by ELISA for IgG antibodies against whole-cell antigen preparations from three strains of O. tsutsugamushi. Data were further analyzed for factors associated with seropositivity including spatial clustering.ResultsSerum samples from 202 dogs (104 urban, 98 rural) were tested for IgG against O. tsutsugamushi, of which 43 (21.3%) were positive. Seroprevalence rates were higher in rural than in urban settings (p<0.01) and in older compared to younger dogs (p<0.01). Spatial analysis by LISA indicated the presence of four localities of highly grouped cases.ConclusionsThe detected seroprevalence supports the endemicity of scrub typhus in southern Chile and suggests a wide exposure of household dogs to the infected, yet unknown vector(s). The spatial data will be used for future research identifying further human cases as well as the local vector(s)/reservoirs for scrub typhus in southern Chile. The study reinforces that dogs are useful sentinels for Orientia spp. in regions of uncertain endemicity and distribution.

Diurnal pattern of pre-weaning den visits and nursing in breeding pairs of captive dingoes (Canis dingo)

The period before pups are weaned represents a key developmental stage for canids that is directly related to the survivability of the pack. Yet our understanding of the role of the parents during this period when pups are confined to a den is rather limited. We sought further insight into this period by observing diurnal patterns of pre-weaning den visits and nursing behaviour in a captive population of dingoes (Canis dingo). We continuously video-monitored behaviour at dens of four captive, genetically pure, dingo pairs (one litter each) during the first three postpartum weeks just before the start of weaning. Mothers occupied the den almost continuously during the night, but significantly less so during the day, and consistently spent most den time nursing. Fathers were largely absent from inside the den despite lack of apparent aggression from females, low outside temperatures, and space for them inside. They spent a large percentage of time on top of the den, suggestive of sentinel duty, although further experiments are necessary to substantiate this. Although limited to captive animals, our observations are consistent with scant reports of bi-parental care in wild dingoes and with suggestions in the literature that reduced parental care in household and free-ranging domestic dogs might be, at least partly, due to the weakening of bi-parental care during their long history of close association with and dependence on humans.

Molecular Screening and Genotyping of Cryptosporidium Species in Household Dogs and In-Contact Children in Egypt: Risk Factor Analysis and Zoonotic Importance.

Cryptosporidiosis is a parasitic zoonosis implicated in severe diarrhoea in pets and humans. This study aimed to determine the prevalence and genotypes of Cryptosporidium spp. in household dogs and in-contact children, and the risk factors associated with infection in children in Sharkia Province, Egypt. Fecal samples of 100 children (2-12 years old) and 50 dogs (3 months-1 year old) were randomly collected from both rural (children: n = 85, dogs: n = 40) and urban (children: n = 15, dogs: n = 10) households. Initial parasite detection was done by light microscopy, while, genotyping was based on molecular diagnostic assays. The overall prevalence of Cryptosporidium spp. infection in children was 35% using microscopy and 14% using nested polymerase chain reaction (PCR). In dogs, it was 34% using microscopy and 24% using nested PCR. Cryptosporidium spp. from children were identified as distinct genotypes, with the predominance of human genotype I (Cryptosporidium hominis) over the zoonotic genotype II (Cryptosporidium parvum). Moreover, only zoonotic genotype II (C. parvum) was identified in dog samples. The significant risk factors associated with the prevalence of Cryptosporidium infection in children were the presence of diarrheal episodes during time of survey, improper disposal of garbage, and dog feces and contact with other livestock (p ≤ 0.05). This study concluded that the existence of C. parvum in children and dogs residing the same households confirm the zoonotic transmission and its public health significance. Also, the study recommended the necessity of hygienic disposal of dog feces and preventing direct contact of dogs with other livestock.

Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece.

Extended-spectrum cephalosporin- and/or carbapenem-resistant (ESCR and/or CarbR) Enterobacteriaceae constitute a public health hazard because of limited treatment options and are endemic among humans in Greece. Recently, ESCR and CarbREnterobacteriaceae have been increasingly isolated from companion animals, stressing their potential role as a reservoir for humans. However, the presence of ESCR bacteria in companion animals within Greek households has not been determined yet. Genes conferring the ESCR and CarbR phenotype were detected among canine isolates and their chromosomal or plasmid location was determined. Standard methods were applied for plasmid characterization. The clonal relatedness of the recovered isolates was examined by multilocus sequence typing (MLST). Here, we report the first findings on the presence of ESCREnterobacteriaceae in healthy Greek dogs. ESCREscherichia coli isolates were associated with different sequence types (STs), including the human pandemic ST131 clone. The occurrence of human-related ESBL/pAmpC genes, plasmid types and/or strain STS in this animal reservoir suggests possible bilateral transmission.

Endoparasites in household and shelter dogs from Central Italy

Intestinal and respiratory parasites are among the most common pathogens in dogs and some of them are recognized as zoonotic agents. In Italy, various taxa have been reported, with variable prevalence estimates depending on study area, dog category and coprological exam technique. In this paper, we report the results of six years of passive surveillance. In the period January 2006-December 2012, 2,775 dog faecal samples from Lazio Region (Central Italy), were examined for parasites, 1,156 from household and 1,619 from shelter dogs, respectively. The following parasites were detected: Giardia duodenalis, Cystoisospora sp., Ancylostomatidae, Toxocara canis, Toxascaris leonina, Trichuris vulpis, Eucoleus böhmi, Eucoleus aerophilus, Mesocestoides sp., Taeniidae and Dipylidium caninum. Helminths were more frequent than protozoa, with total prevalences of 29.1% and 10.7%, respectively. T. vulpis and Ancylostomatidae were the most common parasites, with prevalences of 9.9% and 9.6% respectively. T. vulpis and Ancylostomatidae were significantly more prevalent in shelter dogs than in household ones. T. canis and Cystoisospora sp. were significantly more frequent in household dogs.The assessment of the prevalence in sheltered and in household dogs is useful to infer the occurrence of different parasites in the origin population and to plan possible control intervention.

Seroprevalence of Neospora caninum-specific antibodies in German breeding bitches

BackgroundNeospora caninum is an intracellular obligate apicomplexan parasite responsible for multisystemic lesions in dogs. Being definitive hosts and reservoirs, dogs excrete environmentally resistant oocysts. Breeding bitches represent a susceptible dog group and infected bitches may spread this parasite through transplacental transmission.ResultsA total of 218 serum samples of German breeding bitches were collected to determine the presence of N. caninum. Antibodies were detected in 16 (7.33%) bitches using a commercial indirect enzyme-linked immunosorbent assay (ELISA). Immunoblotting analysis confirmed all seropositive samples detected by ELISA, proving that the animals were infected with N. caninum. The owners were interviewed regarding breed, age, environment, type, vaccine status, feeding habits and the presence of reproductive disorders. Seropositive animals were between the ages of two to seven years; three of them were kept in kennels while the others were household dogs, one of which was additionally a hunting dog. Owners of four seropositive bitches reported one gestation, while multiple pregnancies had been recorded for the other twelve bitches. Fourteen bitches were regularly vaccinated and six were fed with fresh raw meat.ConclusionsAlthough the results confirmed a low incidence of N. caninum seropositive German breeding bitches, further epidemiological and surveillance studies are required to complement our findings regarding the current situation of neosporosis in this specific canine population of Germany.