For certain experiments involving the use of the tropical rat mite, Liponyssus bacoti, as vector of the cotton rat filaria, Litomosoides carinii, it has been necessery to make rapidly available several thousand adult mites from a single source. A method for producing the required number of mites by raising them on a white rat or a cotton rat in a metal tank containing wood shavings and hay has already been described (Scott, Stembridge, and Sisley, 1947). The apparatus shown in Fig. 1 has been constructed for the purpose of extracting within a single working day this large number of adult mites and the even larger number of accompanying nymphs from approximately 4000 cc of wood shavings and an equal volume of hay. The principal on which the apparatus is based is that used by Bertram (1946) in an apparatus for removing mites from small quantities of sawdust, namely that of driving the mites ahead of a wave of heat. Because of the problem of condensation of moisture the closed system of Bertram was replaced by an open trough of galvanized sheet iron surrounded by a moat containing water. Instead of a hot wire heating element there was substituted an infra red bulb of 250 watts, the back of which is reflector coated. This bulb is mounted on a carriage containing two pulleys which run on a wood track at a sufficient height above the trough to cause the principal heating area to slightly more than cover the width of the trough. The motive power is provided by a small electric motor turning a glass tube. A heavy cotton cord from the lamp carriage is wound twice around this tube, then passed through a pulley and attached to a weight. The length of the cord is adjusted so that the weight will rest on the floor just before the lamp reaches the end of the tank, relieving the tension and allowing the cord to slip. Thus if the operator is absent there will be no damage to the mechanism and any mites remaining at the end of the trough will not be killed by excessive application of heat. The lamp moves at the rate of ? inch per hour and will heat a 1 inch layer of sawdust or a 4-inch layer of loose hay. It was found that by the time mites stranded on the end of a piece of hay were forced to drop off, the metal bottom was so hot that they were killed. The bottom of the trough was therefore lined with a ?-inch sheet of hard asbestos-cement composition board to delay conduction of heat along the bottom. An auxiliary hot wire heater can be hung from the lamp carriage beneath the trough and just behind the center of the lamp so as to heat a thicker layer of sawdust from beneath. When the mites are not too numerous they can be removed from the inner top edge of the trough by means of a suction bottle. One hole of the rubber stopper in this bottle is covered with bolting silk and attached by rubber and glass tubes to a suction pump, while the other hole is provided with a tapered and bent glass tube into which the mites are sucked. Some mites will fall into the water and can be
Read full abstract