Hot Springs Research Articles

Results of the CO2 diffuse degassing survey from the 2017 IAVCEI CCVG 13th volcanic gas workshop: Pululahua Dome Complex, Ecuador

Pululahua is a potentially active andesite and dacite lava dome complex. This paper presents the results of a survey focused on carbon dioxide (CO2) diffuse degassing at Pululahua, which was conducted during the 2017 International Association of Volcanology and Chemistry of the Earth's Interior (IAVCEI) Commission of the Chemistry of Volcanic Gases (CCVG) 13th Gas Workshop. Our objective was to conduct a comprehensive investigation of CO2 diffuse degassing by employing standard methods for measuring CO2 flux and temperature, and data processing. These methods were applied to map the spatial distribution of the measured parameters, investigate the origin of CO2, and quantify the volcanic CO2 output within the surveyed area of Pululahua. We carried out a total of 350 soil CO2 flux and 329 soil temperature measurements and collected 12 gas samples for carbon isotopic composition analysis, surrounding the three youngest domes in the complex. In addition, seventeen CO2 flux measurements over a thermal water pool were performed. Our findings indicate that the diffuse emission at Pululahua's crater floor is fed by both biogenic and volcanic CO2. Fluxes from each source are similar in magnitude, with approximately 90% of the measurements falling into an intermediate flux range. The occurrence of volcanic CO2 emissions is supported by the carbon isotopic composition. Diffuse degassing distribution highlights a CO2 anomaly surrounding the younger domes within the crater. We estimated the CO2 diffuse emission using both statistical and geostatistical approaches over area of 3.36 km2, resulting in values of 154.2 t d−1 and 126.2 t d−1 respectively. Based on the geostatistical quantification of the total CO2 emission from soil degassing, Pululahua's crater volcanic CO2 contribution is estimated between 59 and 97 t d−1. Finally, the potential hazards associated with the release of cold CO2 at Pululahua's crater are also discussed.

Molecular Screening for Cyanobacteria and Their Cyanotoxin Potential in Diverse Habitats.

Cyanobacteria are adaptable and dominant organisms that exist in many harsh and extreme environments due to their great ecological tolerance. They produce various secondary metabolites, including cyanotoxins. While cyanobacteria are well studied in surface waters and some aerial habitats, numerous other habitats and niches remain underexplored. We collected 61 samples of: (i) biofilms from springs, (ii) aerial microbial mats from buildings and subaerial mats from caves, and (iii) water from borehole wells, caves, alkaline, saline, sulphidic, thermal, and iron springs, rivers, seas, and melted cave ice from five countries (Croatia, Georgia, Italy, Serbia, and Slovenia). We used (q)PCR to detect cyanobacteria (phycocyanin intergenic spacer-PC-IGS and cyanobacteria-specific 16S rRNA gene) and cyanotoxin genes (microcystins-mcyE, saxitoxins-sxtA, cylindrospermopsins-cyrJ), as well as amplicon sequencing and morphological observations for taxonomic identification. Cyanobacteria were detected in samples from caves, a saline spring, and an alkaline spring. While mcyE or sxtA genes were not observed in any sample, cyrJ results showed the presence of a potential cylindrospermopsin producer in a biofilm from a sulphidic spring in Slovenia. This study contributes to our understanding of cyanobacteria occurrence in diverse habitats, including rare and extreme ones, and provides relevant methodological considerations for future research in such environments.

Gas isotope geochemistry of Tangquan hot spring system in Nanjing (China)

Tangquan hot springs is one of the three well-known geothermal systems in Nanjing, Jiangsu Province, eastern China. Previous studies have shown its high flow rate and ideal water chemistry favorable for utilization. Here we further examine the potential of the hot springs using gas geochemistry in this paper. 35 water samples and 16 gas samples were collected for chemical isotopic analyses. The geothermal gases are found to be primarily atmospheric in origin, containing high nitrogen, oxygen and carbon dioxide gases. Carbon isotope shows that the origin of CO2 and CH4 are thermal metamorphic in the upper crust. The isotope ratio of helium is 3.47–4.73 Ra, indicating that mantle He accounts for 44–60% in total of geothermal gases. These findings furnish the grounds to infer geothermal potential at the deeper part of the geothermal system. The immediate geothermal reservoir has a temperature of 90 °C, but the temperature of deeper part of the system could reach 150–205 °C, using the carbon gas isotope geothermometer. These findings show a promising future of deep geothermal resources in the region.

A hybrid building-integrated solar-powered anaerobic digestion system: Energy, economic, and environmental analysis in Morocco

In this study, we propose a hybrid solar-anaerobic digestion system for a cleaner energy in the building sector. This system offers sustainable treatment of organic waste, utilizing waste generated by residential buildings. We conducted an investigation into the energy, economic, and environmental aspects of integrating a hybrid solar anaerobic digester treating kitchen waste (KW) and blackwater (BW) into Moroccan residential buildings under different climatic conditions. Two building categories were considered: buildings compliant with Moroccan thermal regulations of construction (MTRC), and non-compliant buildings, which represent the majority in Morocco. Results indicate that the proposed hybrid system, utilizing only waste generated by the building, can, on average, satisfy approximately 26 % of the total heating, cooling, and electricity needs of the considered MTRC non-compliant buildings. As for the MTRC compliant buildings, about 30 % of the total energy needs are met. Simulation outcomes also demonstrate that increasing the quantity of organic waste enhances system autonomy, allowing it to meet 100 % of the energy demand of the studied buildings. Excess heat energy generated by the hybrid system was allocated to the buildings' thermal and domestic hot water demands. Furthermore, the study highlights the significant influence of fixed costs and waste quantity on the system's profitability. Environmental benefits were also evident, with the designed hybrid system reducing CO2 emissions by 66 % compared to using grid electricity.

Ignisphaera cupida sp. nov., a hyperthermophilic hydrolytic archaeon from a hot spring of Uzon (Kamchatka), and emended description of the genus Ignisphaera

A novel strictly anaerobic hyperthermophilic archaeon, strain 4213-coT, was isolated from a terrestrial hot spring in the Uzon Caldera, Kamchatka (Russian Federation). Coccoid cells were present singly, in pairs, or aggregates, and occasionally were motile. The strain grew at 75–100 °C and within a pH range of 5.4–8.2 with the optimum at 92 °C and pH 6.4–6.7. Strain 4213-coT was a chemoorganoheterotroph, growing on proteinaceous substrates and mono-, di- and polysaccharides (starch, guar gum, xanthan gum). It did not require sodium chloride for growth. The complete genome of strain 4213-coT was 1.74 Mbp in size; its G+C content was 36.18 %. Genome analysis allowed to identify 25 genes encoding glycosidases involved in polysaccharide hydrolysis as well as genes of ADP-forming acetate-CoA ligase, lactate dehydrogenase and two [NiFe] hydrogenases responsible for acetate, lactate and hydrogen formation during fermentation. Moreover gene cluster encoding archaellum subunits was found. According to the phylogenomic analysis strain 4213-coT formed a species-level phylogenetic lineage within Ignisphaera genus. Our phylogenomic analysis also supports the delineation of the Ignisphaera genus into a separate family Ignisphaeraceae, as recently published. Here we propose a novel species Ignisphaera cupida, sp. nov. with type strain 4213-coT (=JCM 39446T=VKM B-3715T=UQM 41593T). Ecogenomic analysis showed that representatives of the Ignisphaera are thermophilic archaea, the majority of them were found in terrestrial hot springs and deep-sea hydrothermal vents. This study allowed a better understanding of physiology and ecology of Ignisphaeraceae – a rather understudied archaeal group.

Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms.

Microbial mats are stratified communities often dominated by unicellular and filamentous phototrophs within an exopolymer matrix. It is challenging to quantify the dynamic responses of community members in situ as they experience steep gradients and rapid fluctuations of light. To address this, we developed a binary consortium using two representative isolates from hot spring mats, the unicellular oxygenic phototrophic cyanobacterium Synechococcus OS-B' (Syn OS-B') and the filamentous anoxygenic phototroph Chloroflexus MS-CIW-1 (Chfl MS-1). We quantified the motility of individual cells and entire colonies and demonstrated that Chfl MS-1 formed bundles of filaments that moved in all directions with no directional bias to light. Syn OS- B' was slightly less motile but exhibited positive phototaxis. This binary consortium displayed cooperative behavior by moving further than either species alone and formed ordered arrays where both species aligned with the light source. No cooperative motility was observed when a non-motile pilB mutant of Syn OS-B' was used instead of Syn OS-B'. The binary consortium also produced more adherent biofilm than individual species, consistent with the close interspecies association revealed by electron microscopy. We propose that cyanobacteria and Chloroflexota cooperate in forming natural microbial mats, by colonizing new niches and building robust biofilms.

Mechanical, Thermal, and Water Absorption Behavior of Ash Gourd (Benincasa Hispida) Peel Particles Filled Epoxy Composites

Recently, bio-composites have attracted much attention due to their potential applications in various industries. The most notable benefits are the product’s low cost, biodegradability, lightweight, availability, and ability to solve environmental issues. The present research utilizes ash gourd (Benincasa hispida) peel, a food waste, as a filler material to produce epoxy (EP) composites. The effect of ash gourd peel particle percentage (ranging from 0 to 25 wt.%) was studied on the developed composites’ mechanical and thermal properties and water absorption behavior. The maximum tensile strength, flexural strength, and shore D hardness were 47.52 MPa, 2409.17 MPa, and 79.6respectively, when the ash gourd peel was 5% by weight in the composite. It was observed that the mechanical characteristics of manufactured bio-composites are negatively affected by the high concentration of ash gourd peel particles in the epoxy matrix. Also, increasing ash gourd peel particle fraction increases the water absorption of composites when immersed in distilled, sea, and tap water. The composite with 5% filler by weight absorbs water at a minimal rate when immersed in seawater. Thermogravimetric analysis was conducted to investigate the newly developed composite’s thermal behavior. In addition, a morphological examination of the fractured surfaces was carried out with assistance from a scanning electron microscope. The work presents ash gourd peel particles as the potential alternative to be used as filler in composites.

Exploring geothermal resources with the CSAMT and microtremor methods: a case study in Tangquan, Jiangsu Province, China

The development of geothermal energy has received extensive attention because of global energy scarcity and environmental pollution. The Tangquan area is in the north of the Yangtze fold belt, and the lack of magmatic rock development has resulted in a low-temperature type of geothermal reservoir. There are sporadic hot springs and cold springs exposed in the study area, but the uncertain deep geological structure, unknown geothermal reservoir formation mechanism, and lack of detailed exploration data seriously restrict the exploration and development of geothermal resources in the area. Filling underground faults with water can significantly reduce electrical resistivity, while traps filled with warm water can cause a decrease in S-wave velocity. Thus, a new integrated geophysical method, including the controlled source audio-frequency magnetotelluric (CSAMT) and microtremor methods, is applied for geothermal exploration in the region. The combination of CSAMT and microtremor methods can determine thermal-controlled and water-conducting structures more effectively and locate geothermal storage more accurately. The source, channel, storage, and cover of regional geothermal reservoir formation are analyzed using geophysical and geological data. That is, the regional NE- and NW-trending faults are explained as thermal-controlled and water-conducted structures, respectively. The deep Sinian Dengying Formation supplies a heat- and water-bearing space. The tectonic intersection area, especially the tensional fault zone, is found to be closely related to the existence of geothermal reservoirs, which is well verified by later drilling results. Finally, a geothermal reservoir model is established to comprehensively understand the distribution of geothermal energy in the region.

Cultivation and visualization of a methanogen of the phylum Thermoproteota.

Methane is the second most abundant climate-active gas, and understanding its sources and sinks is an important endeavour in microbiology, biogeochemistry, and climate sciences1,2. For decades, it was thought that methanogenesis, the ability to conserve energy coupled to methane production, was taxonomically restricted to a metabolically specialized group of archaea, the Euryarchaeota1. The discovery of marker genes for anaerobic alkane cycling in metagenome-assembled genomes obtained from diverse habitats has led to the hypothesis that archaeal lineages outside the Euryarchaeota are also involved in methanogenesis3-6. Here we cultured Candidatus Methanosuratincola verstraetei strain LCB70, a member of the archaeal class Methanomethylicia (formerly Verstraetearchaeota) within the phylum Thermoproteota, from a terrestrial hot spring. Growth experiments combined with activity assays, stable isotope tracing, and genomic and transcriptomic analyses demonstrated that this thermophilic archaeon grows by means of methyl-reducing hydrogenotrophic methanogenesis. Cryo-electron tomography revealed that Ca. M. verstraetei are coccoid cells with archaella and chemoreceptor arrays, and that they can form intercellular bridges connecting two to three cells with continuous cytoplasm and S-layer. The wide environmental distribution of Ca. M. verstraetei suggests that they might play important and hitherto overlooked roles in carbon cycling within diverse anoxic habitats.

Identifikasi Struktur Bawah Permukaan Menggunakan Geomagnet Wilayah Hot Spring Lau Sigembura Deli Serdang

This research was conducted at the Lau Sigembura hot spring tour, STM Hilir sub-district, Deli Serdang district using the magnetic method. This study aims to determine. Subsurface structures and the distribution of magnetic anomalies which are geothermal manifestations. The number of measurement points is 50 with an area of ​​1000 square meters. Data acquisition was carried out using the PPM Model GEM System type GSM 19T.While data processing uses daily corrections and IGRF corrections as well as upward continuations. Modeling is done using Mag2DC software. The total magnetic field ranges from -900 nT to 500 nT based on the contour of the local magnetic anomaly, the variation in the value of the anomaly is around -1050 nT to 350 nT. While the quantitative interpretation indicates the presence sandstone rock with susceptibility value 0,1075 cgs, Andesit with susceptibility values 0,0183 cgs, limestone with a susceptibility value of 0.0001 cgs – 0.0021 cgs. And shales with susceptibility value 0,021 cgs.

Phylogenomic analyses and comparative genomic studies of Thermus strains isolated from Tengchong and Tibet Hot Springs, China.

Genus Thermus is the main focus of researcher among the thermophiles. Members of this genus are the inhabitants of both natural and artificial thermal environments. We performed phylogenomic analyses and comparative genomic studies to unravel the genomic diversity among the strains belonging to the genus Thermus in geographically different thermal springs. Sixteen Thermus strains were isolated and sequenced from hot springs, Qucai hot springs in Tibet and Tengchong hot springs in Yunnan, China. 16S rRNA gene based phylogeny and phylogenomic analyses based on concatenated set of 971 Orthologous Protein Families (supermatrix and gene content methods) revealed a mixed distribution of the Thermus strains. Whole genome based phylogenetic analysis showed, all 16 Thermus strains belong to five species; Thermus oshimai (YIM QC-2-109, YIM 1640, YIM 1627, 77359, 77923, 77838), Thermus antranikianii (YIM 73052, 77412, 77311, 71206), Thermus brokianus (YIM 73518, 71318, 72351), Thermus hydrothermalis (YIM 730264 and 77927) and one potential novel species 77420 forming clade with Thermus thalpophilus SYSU G00506T. Although the genomes of different strains of Thermus of same species were highly similar in their metabolic pathways, but subtle differences were found. CRISPR loci were detected through genome-wide screening, which showed that Thermus isolates from two different thermal locations had well developed defense system against viruses and adopt similar strategy for survival. Additionally, comparative genome analysis screened competence loci across all the Thermus genomes which could be helpful to acquire DNA from environment. In the present study it was found that Thermus isolates use two mechanism of incomplete denitrification pathway, some Thermus strains produces nitric oxide while others nitrious oxide (dinitrogen oxide), which show the heterotrophic lifestyle of Thermus genus. All isolated organisms encoded complete pathways for glycolysis, tricarboxylic acid and pentose phosphate. Calvin Benson Bassham cycle genes were identified in genomes of T. oshimai and T. antranikianii strains, while genomes of all T. brokianus strains and organism 77420 were lacking. Arsenic, cadmium and cobalt-zinc-cadmium resistant genes were detected in genomes of all sequenced Thermus strains. Strains 77,420, 77,311, 73,518, 77,412 and 72,351 genomes were found harboring genes for siderophores production. Sox gene clusters were identified in all sequenced genomes, except strain YIM 730264, suggesting a mode of chemolithotrophy.Through the comparative genomic analysis, we also identified 77420 as the genome type species and its validity as novel organism was confirmed by whole genome sequences comparison. Although isolate 77420 had 99.0% 16S rRNA gene sequence similarity with T. thalpophilus SYSU G00506T but based on ANI 95.86% (Jspecies) and digital DDH 68.80% (GGDC) values differentiate it as a potential novel species. Similarly, in the phylogenomic tree, the novel isolate 77,420 forming a separate branch with their closest reference type strain T. thalpophilus SYSU G00506T.

Exploring the Origins and Evolution of Oxygenic and Anoxygenic Photosynthesis in Deeply Branched Cyanobacteriota.

Cyanobacteriota, the sole prokaryotes capable of oxygenic photosynthesis (OxyP), occupy a unique and pivotal role in Earth's history. While the notion that OxyP may have originated from Cyanobacteriota is widely accepted, its early evolution remains elusive. Here, by using both metagenomics and metatranscriptomics, we explore 36 metagenome-assembled genomes from hot spring ecosystems, belonging to two deep-branching cyanobacterial orders: Thermostichales and Gloeomargaritales. Functional investigation reveals that Thermostichales encode the crucial thylakoid membrane biogenesis protein, vesicle-inducing protein in plastids 1 (Vipp1). Based on the phylogenetic results, we infer that the evolution of the thylakoid membrane predates the divergence of Thermostichales from other cyanobacterial groups and that Thermostichales may be the most ancient lineage known to date to have inherited this feature from their common ancestor. Apart from OxyP, both lineages are potentially capable of sulfide-driven AnoxyP by linking sulfide oxidation to the photosynthetic electron transport chain. Unexpectedly, this AnoxyP capacity appears to be an acquired feature, as the key gene sqr was horizontally transferred from later-evolved cyanobacterial lineages. The presence of two D1 protein variants in Thermostichales suggests the functional flexibility of photosystems, ensuring their survival in fluctuating redox environments. Furthermore, all MAGs feature streamlined phycobilisomes with a preference for capturing longer-wavelength light, implying a unique evolutionary trajectory. Collectively, these results reveal the photosynthetic flexibility in these early-diverging cyanobacterial lineages, shedding new light on the early evolution of Cyanobacteriota and their photosynthetic processes.

Aeromagnetic Data Analysis of Geothermal Energy Potential of a Hot Spring Area in Western Saudi Arabia

Aeromagnetic Data Analysis of Geothermal Energy Potential of a Hot Spring Area in Western Saudi Arabia

Satellite land surface temperature for mapping the structurally controlled geothermal anomalies in the Cretaceous rift, South Egypt

ABSTRACT Exploration of new geothermal systems often requires regional-scale investigation. Satellite Land Surface Temperature (LST) products are essential in geothermal anomaly identification and mapping. In this study, seven thermal anomalies covering Egypt were observed using the Sentinel-3 LST images acquired in 2019 and confirmed using the 2020 LST images. In addition, the LST analysis reveals three new anomalies covering the southern part of Egypt (Kalabsha, Natash, and Wadi Allaqi), which are structurally controlled. Nuqra-Kharit-Natash is a good site for geothermal energy owing to the following reasons: 1) the high geothermal gradient beneath the Wadi Natash region (heat flow ~100 mW/m2); 2) the presence of many geothermal anomalies retrieved from the analyses of the Landsat-8 acquired in August 2018; 3) existence of surface geothermal manifestation at the western boundary of the basin includes warm springs and the tufa deposits. Intricate structural patterns, including NW-oriented normal faults, ENE-to-WNW-oriented right-lateral faults, and NNE-oriented sinistral strike-slip faults, characterise Wadi Natash. Wadi Natash LST anomaly shows a solid relation to fault trends. High values of LST are arranged along NW and NNE faults and recorded the peak at the intersection of two fault trends. Recent seismic activity along the NNE fault system enhances the permeability along this trend and its intersection with the NW-oriented fault.

Fluid upwelling and alluvial controls on spring localization: An example from Sri Lanka

In water-scarce regions, perennial springs can be a valuable source of drinking water. However, to identify unreported springs and shallow water upwelling zones, it is essential to understand the factors that control spring localization. In a crystalline basement, as in Sri Lanka, without a sedimentary cover, faults and fractures provide the only far-reaching fluid pathways and springs commonly emerge at fault/fracture intersections. While surveying cold and hot water springs in Sri Lanka, it was observed that all springs probed were located at the edge of alluvium. In order to gain insight into this relationship, we performed a topographic and geomorphological analysis was conducted utilizing remote sensing, geological and soil maps, and geological mapping in the field. The results of our analysis of 27 springs indicate that their localization is controlled by fault intersections, non-permeable clay in the alluvium and laterite, and the chemically weathered surface of the bedrock. Furthermore, the constant discharge rates observed over the years and isotope analysis suggest that the springs are part of a tens-of-kilometer-wide regional groundwater system. Based on these results, we propose a conceptual model in which water rises at fault intersections from depth until it reaches the base of the alluvium where up to several meters thick clay with low to zero permeability further inhibits vertical flow forcing the water to spread laterally. Along the alluvium clay boundary with the more permeable weathered bedrock, the water continues its path to the surface. The localization of springs differs from that of fault intersection by tens of meters, with the potential for mixing between shallow and deep groundwater. This observed effect of alluvium and their contact boundaries on spring localization has not been reported for Sri Lanka. Consequently, discharge rates may be significantly increased if the fault intersections are specifically targeted by shallow drilling.

Unveiling the Role of Minerals and Trace Elements of Thermal Waters in Skin Health

Unveiling the Role of Minerals and Trace Elements of Thermal Waters in Skin Health

A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products

In pharmaceutical manufacturing, ensuring product safety involves the detection and identification of microorganisms with human pathogenic potential, including Burkholderia cepacia complex (BCC), Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, Clostridium sporogenes, Candida albicans, and Mycoplasma spp., some of which may be missed or not identified by traditional culture-dependent methods. In this study, we employed a metagenomic approach to detect these taxa, avoiding the limitations of conventional cultivation methods. We assessed the groundwater microbiome’s taxonomic and functional features from samples collected at two locations in the spring and summer. All datasets comprised 436–557 genera with Proteobacteria, Bacteroidota, Firmicutes, Actinobacteria, and Cyanobacteria accounting for > 95% of microbial DNA sequences. The aforementioned species constituted less than 18.3% of relative abundance. Escherichia and Salmonella were mainly detected in Hot Springs, relative to Jefferson, while Clostridium and Pseudomonas were mainly found in Jefferson relative to Hot Springs. Multidrug resistance efflux pumps and BlaR1 family regulatory sensor-transducer disambiguation dominated in Hot Springs and in Jefferson. These initial results provide insight into the detection of specified microorganisms and could constitute a framework for the establishment of comprehensive metagenomic analysis for the microbiological evaluation of pharmaceutical-grade water and other non-sterile pharmaceutical products, ensuring public safety.

Comparative assessment of thermal oils and water as working fluids in parabolic trough collectors for enhanced solar power generation

Examining the thermal behavior of specific working fluids, namely Syltherm800 and TherminolVP-1, in parabolic trough collectors (PTCs) is imperative for enhancing power generation. This study addresses a crucial gap by conducting computational fluid dynamics simulations through COMSOL Multiphysics software and experimental tests to explore the advantages of utilizing thermal oils over water as a working fluid in PTCs. Experimental tests were performed on a water-based PTC in Iraq to validate the numerical model, considering various operating conditions such as input temperature (323.15–423.15 K) and mass flow rates (0.00926–0.0556 kg/s). Key parameters including output temperature, thermal efficiency, useful heat, and total heat losses were evaluated. The numerical model was validated against experimental data, showing good agreement with an overall discrepancy of 1.7% for the current experiments and 3.18% for literature results. The results indicated that Syltherm800, particularly with a high mass flow rate, outperformed TherminolVP-1 and water in terms of overall thermal performance. The optimal PTC thermal efficiency was achieved in July with a mass flow rate of 0.0556 kg/s and an input temperature of 348.15 K. The optimal range for PTC thermal efficiency over four months was between 50% and 70%. The endorsement of thermal oils in PTCs is supported by their low vapor pressure, superior thermal stability, and extended lifespan.

The analysis of potentially toxic heavy metal contamination in the Lake Bogoria geothermal springs

Lake Bogoria geothermal system is a natural geological site in Kenya that boasts a habitat for Flamingos which attract tourists, and its water find applications in agriculture and recreation. Accordingly, its geological processes, closed water systems and anthropogenic activities may lead to accumulation of potential toxic metal contamination. The present study focused on the analysis of potentially toxic metals — manganese (Mn), cadmium (Cd), lead (Pb), copper (Cu) and nickel (Ni) using atomic absorption spectrometry (AAS) in geothermal spring water samples after acid digestion method, and evaluated their health risks through dermal and ingestion exposures. Principal component analysis (PCA), enrichment factors (EF) and Pearson correlation coefficient (PCC) were performed to determine the sources and correlation between the metals investigated. Manganese, Cu and Ni recorded mean concentrations (ppm) of 0.19 ± 0.08, 0.02 ± 0.02 and 0.03 ± 0.02, respectively, which were below the permissible World Health Organisation (WHO) limits, whereas Pb (0.06 ± 0.04) and Cd (0.05 ± 0.02) levels were above the threshold standards. PCA revealed that these metals may have originated from geological regimes and surface run-offs. Copper and Cd metals were highly enriched indicating an anthropogenic origin. The Hazard quotients (HQ) of Pb and Cd were >1,\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$>1,$$\\end{document} hence they are potential precursors for non-carcinogenic and carcinogenic risks in both children and adults. The findings of this study showed possible Pb and Cd metal contamination from geothermal waters. Therefore, there is need for remediation, continuous monitoring and assessment of geothermal waters before utilization for recreation, crop production and geothermal energy exploitation to ensure environmental conservation and human safety.

Plant growth promotion activities of Bacillus spp. isolated from Jakrem hot water spring of Meghalaya, North East India

The study aims to investigate plant growth promotion (PGP) activities of thermophilic bacteria isolated from the Jakrem hot spring in Meghalaya, North-East India, and determine their effect on Brassica juncea's growth. The bacteria were isolated by a culture-dependent approach following a serial dilution method in a nutrient agar medium. All the isolates were determined for PGP attributes such as indole acetic acid, phosphate solubilization, hydrolytic enzymes, and siderophore production. The potent bacterial isolates were characterized by 16S rDNA sequencing and phylogenetic analysis. Altogether, 53 bacterial isolates were obtained, most belonging to the genus Bacillus. Of the total isolates, 37.7% exhibited both PGP and hydrolytic enzyme activities. Three isolates, namely JAB1, JAB8, and JAB100, showed promising PGP and were identified as Bacillus velezensis, B. proteolyticus, and Bacillus sp., respectively. The PGP attributes of these isolates were determined in vivo on B. juncea, and their effects were measured in terms of shoot and root length biomass and biochemical contents. It was observed that combined inoculation of all three isolates significantly enhanced the growth and development of B. juncea, evident by increased shoot and root length, fresh and dry weight, and higher levels of protein, phenol, flavonoid, and chlorophyll content compared to the control. In conclusion, the study highlights the potential application of thermophilic Bacillus spp. from hot springs as bioinoculants to enhance crop productivity in sustainable agricultural practices.