Horticultural Groups Research Articles

Psychological and physiological benefits of horticultural therapy for Chinese older adults

Background Gardening as a leisure activity has been shown to be potentially beneficial for older adults, this study aimed to assess the physiological and psychological impacts of a specific horticultural activity, watering indoor plants, compared with playing a computer game - the Klondike Solitaire Card Game. Methods This crossover study included 100 older adults recruited from a nursing care facility for older people in Shanghai, China. They were allocated to either engage in watering indoor plants (horticultural group) or playing a computer game (control group). For the inclusion criteria, screening for cognitive function was conducted to exclude those with severe cognitive impairments, thereby focusing on those capable of participating fully in the study activities. Brainwave activities, blood pressure, and psychological states were measured through pre- and post-test questionnaires to evaluate the physiological and psychological effects. Results The activity of watering indoor plants was associated with significant reductions in both systolic and diastolic blood pressure, in contrast to the computer game activity. Additionally, participants in the horticultural group showed increased brainwave mean values, indicating heightened relaxation and focus, while decreases were observed in the control group. The experimental group reported reduced anxiety scores, along with increased feelings of comfort and relaxation, and also exhibited significantly higher meditation levels, which indicates a profound mental relaxation effect. Conclusion Our findings suggest that engaging in indoor horticultural activities, such as watering houseplants for 10 minutes, can reduce mental and physical stress levels in older adults.

The Influence of Horticultural Group Counseling Activities on Mental Health of College Students

The Influence of Horticultural Group Counseling Activities on Mental Health of College Students

Telomere-to-telomere genome assembly of melon (Cucumis melo L. var. inodorus) provides a high-quality reference for meta-QTL analysis of important traits.

Melon is an important horticultural crop with extensive diversity in many horticultural groups. To explore its genomic diversity, it is necessary to assemble more high-quality complete genomes from different melon accessions. Meanwhile, a large number of QTLs have been mapped in several studies. Integration of the published QTLs onto a complete genome can provide more accurate information for candidate gene cloning. To address these problems, a telomere-to-telomere (T2T) genome of the elite melon landrace Kuizilikjiz (Cucumis melo L. var. inodorus) was de novo assembled and all the published QTLs were projected onto it in this study. The results showed that a high-quality Kuizilikjiz genome with the size of 379.2Mb and N50 of 31.7Mb was de novo assembled using the combination of short reads, PacBio high-fidelity long reads, Hi-C data, and a high-density genetic map. Each chromosome contained the centromere and telomeres at both ends. A large number of structural variations were observed between Kuizilikjiz and the other published genomes. A total of 1294 QTLs published in 67 studies were collected and projected onto the T2T genome. Several clustered, co-localized, and overlapped QTLs were determined. Furthermore, 20 stable meta-QTLs were identified, which significantly reduced the mapping intervals of the initial QTLs and greatly facilitated identification of the candidate genes. Collectively, the T2T genome assembly together with the numerous projected QTLs will not only broaden the high-quality genome resources but also provide valuable and abundant QTL information for cloning the genes controlling important traits in melon.

Effect of tetraploidization on morphological and fertility characteristics in Iris × norrisii Lenz

Tetraploids have been widely regarded as important contributors to the development of prime cultivars in many horticultural groups of Iris. These tetraploid plants tend to display intense flower color, increased flower size, and larger growth vitality compared to their diploid progenitors. However, no polyploid has ever been found or induced in the interspecific hybrid Iris × norrisii. In order to obtain new, innovative polyploid germplasm in I. × norrisii, 64 pre-germinated seeds were soaked with 0.05% colchicine, of which 14 survived and 6 flowered in the first growing season. Eight tetraploids were identified from the self-fertilized progeny of one of the six adult plants. The synthetic tetraploid I. × norrisii showed greater viability and developed wider leaves with larger stomata and pollen grains than their diploid counterparts. With tetraploidization, flowers were larger (by 26%), fruits became longer (by 54%), and selfing seeds were a little wider (by 10%) and significantly longer (by 33%) in size. The synthetic tetraploids exhibited a 9.10% selfing fruit-setting rate with 100% plump seeds. Conversely, most seeds collected from the interploidy cross between tetraploids and diploids were empty, with only 4.11% being plump. As a rare Iris species capable of flowering in the first growing season, the induced tetraploid I. × norrisii offers great potential to produce more genetically diverse progenies in a relatively short time, and thus provides a foundation for further research into polyploidization in Iris.

Analysis of genetic diversity and population structure in Cambodian melon landraces using molecular markers

Genetic diversity of Cambodian melons was evaluated by the analysis of 12 random amplified polymorphic DNA (RAPD) and 7 simple sequence repeat (SSR) markers using 62 accessions of melon landraces and compared with 231 accessions from other areas for genetic characterization of Cambodian melons. Among 62 accessions, 56 accessions were morphologically classified as small-seed type with seed lengths shorter than 9 mm, as in the horticultural groups Conomon and Makuwa. Gene diversity of Cambodian melons was 0.228, which was equivalent to those of the groups Conomon and Makuwa and smaller than those of Vietnamese and Central Asian landraces. A phylogenetic tree constructed from a genetic distance matrix classified 293 accessions into three major clusters. Small-seed type accessions from East and Southeast Asia formed clusters I and II, which were distantly related with cluster III consisting of large-seed type melon from other areas. All Cambodian melons belonged to cluster I (except three accessions) along with those from Thailand, Myanmar, Yunnan (China), and Vietnam (“Dua thom” in the northwest), thus indicating genetic similarity in these areas. In addition, the Cambodian melons were not differentiated among geographical populations. Conomon and Makuwa were classified into cluster II, together with melon groups from the plains of Vietnam. The presence of two groups of melons in Southeast Asia was also indicated by population structure and principal coordinate analysis. These results indicated a close genetic relationship between Cambodia and the neighboring countries, thus suggesting that Cambodian melons are not directly related to the establishment of Conomon and Makuwa.

Effects of Horticultural Activities on Mild to Moderate Depression Symptoms: A Randomized Controlled Trial

Objectives This study was designed to evaluate the effect of participating in horticultural activities on changes in the severity of depression symptoms in adults with mild-to-moderate depression symptoms. Methods In this non-blinded, randomized controlled trial, 102 eligible participants were assigned randomly to the experimental and control groups (51 members each). The inclusion criteria were as follows: age of 18 years or more, mild-to-moderate depression symptoms, and willingness to participate in the study. However, the exclusion criteria were physical or mental diseases limiting horticultural activities and using antidepressants. Both groups received educational worksheets, including behavioral activation, daily activity schedules, breathing training, and relaxation instructions. Moreover, the experimental group participated in weekly three-hour horticultural program sessions for six weeks. The severity of depression symptoms was then appraised at baseline, at the end of the intervention, and four weeks after the end of the intervention through the Beck depression inventory-II (BDI-II). The repeated-measures ANOVA was used for data analysis. Results The results revealed that the mean BDI-II scores in the horticultural group declined by 5.68 (P=0.001) and 2.32 (P=0.016) points more than the control group at the end of the intervention and four weeks later, respectively. Conclusion This study indicated that horticultural activity might mitigate the severity of depression symptoms.

Pan-genome and multi-parental framework for high-resolution trait dissection in melon (Cucumis melo).

Linking genotype with phenotype is a fundamental goal in biology and requires robust data for both. Recent advances in plant-genome sequencing have expedited comparisons among multiple-related individuals. The abundance of structural genomic within-species variation that has been discovered indicates that a single reference genome cannot represent the complete sequence diversity of a species, leading to the expansion of the pan-genome concept. For high-resolution forward genetics, this unprecedented access to genomic variation should be paralleled and integrated with phenotypic characterization of genetic diversity. We developed a multi-parental framework for trait dissection in melon (Cucumis melo), leveraging a novel pan-genome constructed for this highly variable cucurbit crop. A core subset of 25 diverse founders (MelonCore25), consisting of 24 accessions from the two widely cultivated subspecies of C. melo, encompassing 12 horticultural groups, and 1 feral accession was sequenced using a combination of short- and long-read technologies, and their genomes were assembled de novo. The construction of this melon pan-genome exposed substantial variation in genome size and structure, including detection of ~300 000 structural variants and ~9 million SNPs. A half-diallel derived set of 300 F2 populations, representing all possible MelonCore25 parental combinations, was constructed as a framework for trait dissection through integration with the pan-genome. We demonstrate the potential of this unified framework for genetic analysis of various melon traits, including rind color intensity and pattern, fruit sugar content, and resistance to fungal diseases. We anticipate that utilization of this integrated resource will enhance genetic dissection of important traits and accelerate melon breeding.

Horticultural activities can achieve the same affect improvement effect of green exercise: A randomized field controlled trial.

With the deepening of non-drug intervention research on human mental health, more and more attention has been paid to the benefits of horticultural activities and green exercise on physical and psychological health. This study compared the affect improvement between horticultural activities with the same intensity and green exercise and that with or without green plants to verify the value of horticultural activities and green exercise in improving human affect and the importance of green plants. A total of 160 subjects aged 18-26 years (average age 22.5 years) were recruited and randomly divided into a control group, a horticultural activity group with green plants, a horticultural activity group without green plants, and a green exercise group. Demographics, sociological variables, and daily physical activity levels were investigated. Green space at Zhejiang Normal University was selected as the test site. After finishing the preparation work, the subjects sat quietly for 8 min before the pre-test. The horticultural group completed 20 min of horticultural activities {8 min of digging [40%*HRR(heart rate reserve) + RHR(resting heart rate)] + 8 min of transplantation [(50%*HRR + RHR) + 4 min of watering (30%*HRR + RHR)]}. The group returned to a calm state (no less than 20 min) for the post-test. The green exercise group completed a 20-min power bike ride. The activity intensity and activity time of the green exercise group were determined according to the activity intensity and time of the horticultural group. Dependent variables were collected, including blood pressure, positive/negative affects, heart rate variability (RMSSD, SDNN, and LF/HF), and controlled covariate environmental parameters (field temperature, humidity, and noise). (1) A significant difference was observed in the improvement effect except for negative affect between the green horticultural activity group and the green exercise group (F = 3.310; ɳp2 = 0.046; p = 0.037). No significant difference was observed in other affect indicators. (2) In the same pattern of with and without green plant horticultural activity group, the green plant horticultural activity group had a better effect on the improvement of affect, and the two groups had a better negative affect (F = 3.310; ɳp2 = 0.046; p = 0.037), SDNN index of heart rate variability(F = 1.035; ɳp2 = 0.015; p = 0.039), and RMSSD index (F = 2.225; ɳp2 = 0.032; p = 0.014), and no significant difference was observed in the improvement effect of other affect indicators between the two groups. Having green horticulture can give the same intensity as green exercise and affect improvement. Findings suggest that people can choose green exercise or horticultural activities according to their preferences and physical characteristics in the two physical activities. Under the same pattern of horticultural activities, green plants are the key factor in improving the affect of horticultural activities. Choosing suitable plant types in horticultural activities is positively significant in enhancing affect.

Heterosis and combining ability for yield traits and its relationship with gene action in muskmelon (Cucumis melo)

Twenty-one F1 hybrids were developed from seven diverse parental lines of muskmelon (Cucumis melo L.) from two horticultural groups, viz. inodorous and cantalupensis through half-diallel mating system and evaluated at ICARIndian Agricultural Research Institute, New Delhi, during 2017 and 2018. The component of variance for sca (σ2s) was higher than gca component of variance (σ2g) for all the characters, except average fruit weight, which indicated the importance of non-additive gene action for improvement of many traits. The predictability ratio and average degree of dominance was less than 0.5 and more than 1 for all the yield related traits, except average fruit weight, which further confirmed the predominant role of non-additive component of variance. The parent DHM-163 (P1) was identified as good general combiner for earliness, whereas parent DCM-31 (P4) for fruit length, average fruit weight and yield per plant. The parent Pusa Madhuras (P5) also exhibited highest gca effects for number of fruits per plant, number ofprimary branches per plant, fruit diameter and negative gca effects for days to first fruit harvest. The hybrid DCM-31 × Kashi Madhu (P4 × P6) exhibited highest heterotic value for days to first male flower anthesis, days to first female flower anthesis and days to first fruit harvest. Three crosses, DMM-159 × Pusa Madhuras (P3 × P5), DHM-163 × Kashi Madhu (P1 × P6) and DMM-159 × DCM-31 (P3 × P4) were found to be best heterotic combinations for yield related traits. These promising heterotic hybrid combinations may be tested under multi-location trials to assess their potential as commercial hybrids.

Intervention Study of Group Based Psychological Counseling Based on Horticulture on Interpersonal Relationship Disturbance of Vocational College Students

This study has made attempts to explore the intervention effect of psychological counseling techniques by horticulture on the interpersonal disturbance of vocational college students. Twenty vocational college students who scored more than 9 points in the Interpersonal Relationship Integrative Diagnostic Scale (IRIDS) were randomly divided into the intervention group (10 persons who received 5 periods of horticultural group based psychological counseling) and the control group (10 persons who were in the waiting group). After the intervention process, the Interpersonal Relationship Integrative Diagnostic Scale (IRIDS) was used for post-test. The results showed that interpersonal distress decreased significantly in the intervention group (p<0.01), while there was no significant difference in the waiting group. It shows that the group psychological guidance by horticulture has a positive intervention effect on the students' interpersonal disturbance, which is conducive to alleviating the students' interpersonal disturbance.

Genetic characterization and population structure analysis among different horticultural groups of muskmelon (Cucumis meloL.) using microsatellite markers

AbstractMuskmelon, which is an important cucurbit of the tropical and subtropical region of the world, shows great diversity, with six different botanical or horticultural groups and fifteen varietal groups. In this study, a total of fifty (50) simple sequence repeat (SSR) markers were used to investigate the genetic diversity and population structure of 46 muskmelon accessions of different horticultural or varietal groups. Thirty-eight (38) of the fifty SSR markers were highly polymorphic. A total of 99 alleles were generated by the polymorphic markers, with an average of 2.06 alleles per loci. Heterozygosity among accessions for individual loci varied from 0.00 to 0.21, with the highest (0.21) reported for the CMCTN71 marker. The gene diversity and polymorphism information content (PIC) values varied between 0.08 to 0.72 and 0.07 to 0.67, with an average of 0.47 and 0.38, respectively. The primer that showed the highest gene diversity and PIC values was DM0913. The unweighted pair-group method for arithmetic average (UPGMA)-based dendrogram classified all the 46 accessions into two major clusters. Population structure analysis classified 46 muskmelon accessions into 2 subpopulations. The subpopulation I contained 29 accessions from thecantalupensisgroup, and subpopulation II contained 17 accessions frommomordica,inodorusandconomongroups, respectively. Analysis of variance indicated that 18 and 68% of variance was due to subpopulations and differences among individuals, respectively. The present study supports the existence of sufficient variation among musk melon genetic resources in India, and their classification based on molecular markers will be helpful to accelerate the breeding programme for specific traits.

Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers

The study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53 watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty SSR markers were shown to be highly polymorphic and were statistically analyzed using Power Marker and NTSYSPc softwares. A total of 33 alleles were generated by polymorphic SSR markers, on an average of 2.6 alleles per loci. Heterozygosity among accessions for individual loci varied from zero to 0.17 (BVWS02306 marker).The gene diversity and PIC values varied between 0.08 to 0.72 and 0.46 to 0.90, respectively and the primer which had the highest gene diversity and PIC values was BVWS02422. The UPGMA-based dendrogram classified all the 53 accessions into two major clusters at 48 per cent similarity. All 41 cultivated watermelon accessions were grouped in cluster I and the remaining 12 accessions from wild types were grouped in cluster II. Thus, this study highlights the importance of molecular markers in the identification of wild and cultivated lines and their exploitation in future breeding programs. Keywords: Watermelon, genetic diversity, microsatellite, Jaccard similarity coefficient &nbsp

A Comprehensive High-Quality DNA and RNA Extraction Protocol for a Range of Cultivars and Tissue Types of the Woody Crop Avocado.

High-quality DNA and RNA forms the basis of genomic and genetic investigations. The extraction of DNA and RNA from woody trees, like avocado (Persea americana Mill.), is challenging due to compounds which interact with nucleic acids and influence separation. Previously reported methods of DNA and RNA extraction from avocado have issues of low yield, quality and applicability across different cultivars and tissue types. In the current study, methods have been optimised for high-quality DNA extraction from 40 avocado cultivars and RNA extraction from multiple tissue types, including roots, stem, leaves, flowers and fruits. The method is based on the modification of the cetyltrimethylammonium bromide buffer, centred around the specific optimisation of chemicals, such as sodium dodecyl sulphate, polyvinylpyrrolidone, sodium sulphite, polyethylene glycol and β-mercaptoethanol. The DNA extraction method yielded high-molecular weight DNA from the leaf tissue of 40 avocado cultivars belonging to Mexican, Guatemalan and West Indian avocado horticultural groups. The method was further optimised for RNA extraction from different avocado plant parts, enabling extraction using amounts as low as ~10 mg of starting material. The DNA and RNA extracted was successfully used for long- and short-read sequencing and gene expression analysis. The methods developed may also be applicable to other recalcitrant plant species.

Unravelling cucumber resistance to several viruses via genome-wide association studies highlighted resistance hotspots and new QTLs.

The mapping and introduction of sustainable resistance to viruses in crops is a major challenge in modern breeding, especially regarding vegetables. We hence assembled a panel of cucumber elite lines and landraces from different horticultural groups for testing with six virus species. We mapped 18 quantitative trait loci (QTL) with a multiloci genome wide association studies (GWAS), some of which have already been described in the literature. We detected two resistance hotspots, one on chromosome 5 for resistance to the cucumber mosaic virus (CMV), cucumber vein yellowing virus (CVYV), cucumber green mottle mosaic virus (CGMMV) and watermelon mosaic virus (WMV), colocalizing with the RDR1 gene, and another on chromosome 6 for resistance to the zucchini yellowing mosaic virus (ZYMV) and papaya ringspot virus (PRSV) close to the putative VPS4 gene location. We observed clear structuring of resistance among horticultural groups due to plant virus coevolution and modern breeding which have impacted linkage disequilibrium (LD) in resistance QTLs. The inclusion of genetic structure in GWAS models enhanced the GWAS accuracy in this study. The dissection of resistance hotspots by local LD and haplotype construction helped gain insight into the panel's resistance introduction history. ZYMV and CMV resistance were both introduced from different donors in the panel, resulting in multiple resistant haplotypes at same locus for ZYMV, and in multiple resistant QTLs for CMV.

Heterosis and combining ability for fruit quality, mineral nutrients and post harvest traits of muskmelon

Twenty-one F1 hybrids of muskmelon, along with seven parents from two horticultural groups, were evaluated for heterosis and combining ability for fruit quality, mineral nutrients, and post harvest traits, in a half diallel scheme, during the spring-summer and kharif seasons. For all fruit quality, mineral nutrient, and post harvest traits, sca component of variance (σ2s) was greater than the gca component of variance (σ2g), indicating the importance of non-additive gene action for improvement. Kashi Madhu (P6) was the best general combiner for K, Zn, Cu, Ca and Mg content, whereas Pusa Madhuras (P5) for ascorbic acid and Mn content. Besides, the parent DCM-31 (P4) was the best general combiner for TSS and Fe content; Hara Madhu (P7) for acidity and Na content; DHM-162 (P2) for respiration rate and ethylene emission rate. The hybrid, DCM-31 × Pusa Madhuras was found to be the best heterotic combination for ascorbic acid and Fe content. The cross DMM-159 × Pusa Madhuras was the best heterotic hybrid for TSS and Cu content. Similarly, the cross DHM-163 × DMM-159 was superior for respiration rate and ethylene emission rate. The cross-combinations, DCM-31 × Kashi Madhu, DHM-163 × DCM-31, DMM-159 × Hara Madhu, DCM-31 × Hara Madhu, DMM-159 × DCM-31 and DHM-163 × Pusa Madhuras, exhibited highest heterosis for acidity, Na, K, Zn, Mn and Ca content. None of the hybrids excelled in fruit quality, mineral nutrients, and post harvest traits, indicating the importance and need for multiple crossing breeding approaches to improve muskmelon fruit quality, mineral concentration, and post harvest life.

Cantaloupe melon genome reveals 3D chromatin features and structural relationship with the ancestral cucurbitaceae karyotype

SummaryCucumis melo displays a large diversity of horticultural groups with cantaloupe melon the most cultivated type. Using a combination of single-molecule sequencing, 10X Genomics link-reads, high-density optical and genetic maps, and chromosome conformation capture (Hi-C), we assembled a chromosome scale C. melo var. cantalupensis Charentais mono genome. Integration of RNA-seq, MeDip-seq, ChIP-seq, and Hi-C data revealed a widespread compartmentalization of the melon genome, segregating constitutive heterochromatin and euchromatin. Genome-wide comparative and evolutionary analysis between melon botanical groups identified Charentais mono genome increasingly more divergent from Harukei-3 (reticulatus), Payzawat (inodorus), and HS (ssp. agrestis) genomes. To assess the paleohistory of the Cucurbitaceae, we reconstructed the ancestral Cucurbitaceae karyotype and compared it to sequenced cucurbit genomes. In contrast to other species that experienced massive chromosome shuffling, melon has retained the ancestral genome structure. We provide comprehensive genomic resources and new insights in the diversity of melon horticultural groups and evolution of cucurbits.

A Review on the diversity of Melon

The species Cucumis melo L. (melon) is a polymorphic taxon encompassing many botanical and horticultural varieties. Melon fruits exhibit substantial variability in their form, shape, skin characters, flesh color, flesh thickness, sweetness, seed cavity, seed size, etc., which reflects in the market value of melon. This existing diversity in melon germplasm leads botanists to propose different classifications at the specific and infraspecific levels. The majority of classifications are based on morphological and molecular characteristics. Morphologic traits such as flower and fruit characteristics (sex expression, fruit size and shape, fruit skin color, pH, flesh color, taste, aroma, sugar composition, seed size, and shape) were used in the past centuries for melon diversity analysis. Later on, scientists correlate these phenotypic and biochemical traits (Total Soluble Solids, Ascorbic acid content, Titratable acidity, etc.) with genotypic variability by molecular tools. Molecular tools generally used for melon diversity analysis are RAPD, RFLP, AFLP, SSR markers, and Single Nucleotide Polymorphism (SNP). This article emphasizes the progress of studies in the species Cucumis melo L. and its varieties since 1753. Particular attention has been paid to collect data regarding different horticultural groups of the species, and a recent infraspecific classification of the species has also been outlined. Even though the modern molecular techniques provide new insight to melon classification still there is an existing disparity in these classifications, which could be visible to the scientific world.

A Turkish-Speaking Community Program in A Primary Care Psychotherapy Setting: How to Support and Engage A Complex Patient Population

ABSTRACT This paper presents a description of a multigroup-based psychotherapy program for Turkish-speaking individuals provided by an innovative primary care mental health service in London. As of 2021, the project offers two types of therapy: a year-long horticultural group, which is a blend between talking therapy and gardening, and a 20-week psychotherapy group. Both were designed to engage the particularly complex Turkish-speaking patient population in the London Borough of Hackney. The project is grounded in group psychotherapy and borrows elements from different theoretical orientations and therapeutic modalities including community psychology, group analytic therapy, horticultural therapy, attachment theory, and dynamic therapies. Here, we present how the program was created and developed and the specifics of the groups, together with the main themes and dynamics that emerged in the therapeutic process. The program evaluation and outcomes are illustrated by several clinical case vignettes throughout the paper.

Multi-tissue transcriptome analysis of two Begonia species reveals dynamic patterns of evolution in the chalcone synthase gene family

Begonia is an important horticultural plant group, as well as one of the most speciose Angiosperm genera, with over 2000 described species. Genus wide studies of genome size have shown that Begonia has a highly variable genome size, and analysis of paralog pairs has previously suggested that Begonia underwent a whole genome duplication. We address the contribution of gene duplication to the generation of diversity in Begonia using a multi-tissue RNA-seq approach. We chose to focus on chalcone synthase (CHS), a gene family having been shown to be involved in biotic and abiotic stress responses in other plant species, in particular its importance in maximising the use of variable light levels in tropical plants. We used RNA-seq to sample six tissues across two closely related but ecologically and morphologically divergent species, Begonia conchifolia and B. plebeja, yielding 17,012 and 19,969 annotated unigenes respectively. We identified the chalcone synthase gene family members in our Begonia study species, as well as in Hillebrandia sandwicensis, the monotypic sister genus to Begonia, Cucumis sativus, Arabidopsis thaliana, and Zea mays. Phylogenetic analysis suggested the CHS gene family has high duplicate turnover, all members of CHS identified in Begonia arising recently, after the divergence of Begonia and Cucumis. Expression profiles were similar within orthologous pairs, but we saw high inter-ortholog expression variation. Sequence analysis showed relaxed selective constraints on some ortholog pairs, with substitutions at conserved sites. Evidence of pseudogenisation and species specific duplication indicate that lineage specific differences are already beginning to accumulate since the divergence of our study species. We conclude that there is evidence for a role of gene duplication in generating diversity through sequence and expression divergence in Begonia.

Pemberdayaan Kelompok Tani Hortikultura di Lahan Pasir melalui Pemanfaatan Kayambang (Salvinia molesta) sebagai Trichokompos

Lebak swamp weeds such as Kayambang (Salvinia molesta) grow abundantly. They can be used as compost, which effectively improves soil fertility, increasing nutrients N, P, and P K quickly and environmentally friendly. The effectiveness of compost fertilizer needs to be increased by adding indigenous microbes as decomposers and biological agents to control plant diseases. The activities carried out to empower horticultural farmer groups on sandy land in Tanjung Pinang Village, Palangka Raya are through socialization, training in composting with three types of antagonist fungus Trichoderma sp. (Trichocompost), facilitate the procurement of weed chopping machines, assist farmers in horticultural crop cultivation, and increase farmers' independence in self-supporting organic fertilizers. The use of Kayambang as Trichocompost with microbial decomposers and indigenous biological agents is new knowledge for partner farmers. The application of Trichocompost on the demonstration plots shows that eggplant plant growth and yields are excellent, meaning that Trichocompost can improve the fertility of sandy soils. Farmers participating in the training stated that the use of Trichocompost could reduce farming costs because it can substitute for manure that has been used by farmers and can meet the self-help needs of organic fertilizers. Participants wanted an advanced mentoring program because the farmers had not yet mastered the isolation or propagation of biological agents and decomposer microbes.