ABSTRACT Hippotherapy is considered as a beneficial field of activity for Žemaitukas, a Lithuanian native horse breed. The aim of this study is to analyze the suitability of Žemaitukas breed horses for hippotherapy by using a set of tests based on the temperament, behavioral reactions, and zoometric measurements. Results of these tests inform whether the future use of Lithuanian native horses in hippotherapy can ensure their welfare. In total, 32 horses were evaluated. Twenty-nine of the evaluated horses were horses of the Žemaitukas breed that had never been used for hippotherapy, the others were hippotherapy horses representing different breeds, which were considered the control group. As expected, control hippotherapy horses scored better in many tests, thus confirming the suitability of these tests for the evaluation of horses for hippotrapeutic use. Žemaitukas breed horses scored an average of 15.9% less points for the test of temperament and 21.3% less points for “Hippo-test” compared with the hippotherapy horses. Nevertheless, the results suggest that Žemaitukas breed horses could be used in hippotherapy after special training courses.