Hormesis In Plants Research Articles

Beneficial or detrimental? How nickel application alters the ionome of soybean plants

The use of nickel (Ni) in agriculture may represent one of the most significant cases of plant hormesis ever reported, as plants exhibit both positive and negative responses depending on the level of exposure to this element. For a more comprehensive understanding of this effect, the next step is to conduct studies on the dynamics of pre-existing chemical elements in the system (ionomic profile), especially when introducing Ni as a novel nutrient for the plants. This micronutrient is of particular interest to the fertilization of leguminous plants, such as the soybean, due to its additional effects on the biological nitrogen fixation process. This study thus evaluated the influence of five doses of Ni (0.0, 0.5, 1.0, 3.0, and 9.0 mg of Ni kg−1) on the ionomic profile of soybean genotypes using modern quantification techniques. The results revealed that the addition of Ni reduced the concentration of cationic micronutrients manganese (Mn), iron (Fe), zinc (Zn), and copper (Cu), while it increased the concentration of macronutrients nitrogen (N) and magnesium (Mg). The application of Ni also resulted in a reduction of the potentially toxic element aluminum (Al). Correlations were also observed for these elements, indicating that Ni could be a controlling agent in elemental absorption and translocation. The ionome of the leaf tissues exhibited the most significant alterations, followed by the grains, nodules, and roots. Exogenous agronomic doses of Ni proved beneficial for the growth and production of soybean plants, although a genotypic effect was observed. The treatment with 9.0 mg of Ni kg−1, resulted in a new ionomic profile related to toxicity, demonstrating suboptimal plant development. Thus, the application of Ni in appropriate doses had a significant impact on the ionomic profile of soybeans, improving plant development and implying resistance to potentially toxic elements such as Al.

Glyphosate hormesis stimulates tomato (Solanum lycopersicum L.) plant growth and enhances tolerance against environmental abiotic stress by triggering nonphotochemical quenching.

Glyphosate is the most widely applied herbicide in the world. Hormesis caused by low glyphosate doses has been widely documented in many plant species. However, the specific adaptative mechanism of plants responding to glyphosate hormesis stimulation remains unclear. This study focused on the biphasic relationship between glyphosate dose and tomato plant growth, and how glyphosate hormesis stimulates plant growth and enhances tolerance to environmental stress. We constructed a hormesis model to describe the biphasic relationship with a maximal stimulation (MAX) of 162% above control by glyphosate at 0.063 g ha-1. Low-dose glyphosate increased photosynthetic pigment contents and improve photosynthetic efficiency, leading to plant growth stimulation. We also found that glyphosate hormesis enhanced plant tolerance to diuron (DCMU; a representative photosynthesis inhibitor) by triggering the nonphotochemical chlorophyll fluorescence quenching (NPQ) reaction to dissipate excess energy stress from photosystem II (PSII). Transcriptomic analysis and quantitative real-time polymerase chain reaction results revealed that the photosynthesis-antenna proteins pathway was the most sensitive to glyphosate hormesis, and PsbS (encoding photosystem II subunit S), ZEP (encoding zeaxanthin epoxidase) and VDE (encoding violaxanthin de-epoxidase) involved in NPQ played crucial roles in the plant response to glyphosate hormesis. These results provide novel insights into the mechanisms of plant hormesis and is meaningful to the application of glyphosate hormesis in agriculture. © 2024 Society of Chemical Industry.

The intrinsic and extrinsic mechanisms regulated by functional carbon nanodots for the phytoremediation of multi-metal pollution in soils

Functional carbon nanodots (FCNs) were currently demonstrated to regulate plant behavior in the agricultural and environmental areas. However, their regulation mechanisms on the interactions of plant-soil system during phytoremediation remain unrevealed. Here, Solanum nigrum L. was employed to explore the intrinsic and extrinsic mechanisms regulated by FCNs in the phytoremediation of Cd-Pb co-contaminated soils. The mediation of FCNs on metal removal and plant growth showed a hormesis manner, wherein the maximum induction effect was contributed by 15 mg kg-1 FCNs. Cd/Pb removal were enhanced by 8.5% and 31.6%, respectively. Moreover, FCNs reallocate metal distribution in plant by immobilized metals in roots and suppressed metal translocation to leaves. Improving plant growth (by 82.8% for root), stimulating plant hormesis, and activating plant detoxification pathways are the intrinsic mechanism for the phytoremediation smartly regulated by FCNs. Notably, FCNs induced soil enzyme activities that associated with soil nutrients recycling, up-regulated the microbial diversity and the soil immune system, and regulated S. nigrum L. to recruit beneficial microbials in the rhizosphere. The above-mentioned comprehensive improvement of soil micro-environment is the extrinsic mechanism regulated by FCNs. This study provides new insights to evaluate the interactions of nanomaterials with plant-soil system under soil contamination.

The role of the ABF1 gene in regulation of Cd-induced hormesis in Arabidopsis thaliana

Hormesis is important in plant performance in contaminated environments, but the underlying genetic mechanisms are poorly understood. This study aimed at mining key genes in regulating Cd-induced hormesis in Arabidopsis thaliana and verifying their biological function. Hormesis of fresh weight, dry weight, and root length occurred at concentrations of 0.003–2.4, 0.03–0.6, and 0.03–0.6 µM Cd, respectively. Superoxide dismutase and catalase activities, and chlorophyll content displayed inverted U-shaped curves, indicating that the antioxidant defense system and photosynthesis system played roles in hormesis. Based on KEGG pathway analysis with the trend chart of differentially expressed genes and weighted correlation network analysis, the key gene ABF1 in the metabolic pathway of abscisic acid was identified. Subsequently, genetic experiments with wild, overexpressing, and knockdown lines of A. thaliana were conducted to further verify the biological function of ABF1 involving Cd-induced hormesis in A. thaliana. The results revealed that the resistance capability of the overexpressing type to Cd stress was significantly enhanced and implicated that the ABF1 gene is essential for Cd-induced hormesis in A. thaliana. Mining key genes that regulate Cd-induced hormesis in plants and stimulate them could have a transformative impact on the phytoremediation of metal-contaminated environments.

Plant hormesis: Revising of the concepts of biostimulation, elicitation and their application in a sustainable agricultural production

Current research in basic and applied knowledge of plant science has aimed to unravel the role of the interaction between environmental factors and the genome in the physiology of plants to confer the ability to overcome challenges in a climate change scenario. Evidence shows that factors causing environmental stress (stressors), whether of biological, chemical, or physical origin, induce eustressing or distressing effects in plants depending on the dose. The latter suggests the induction of the “hormesis” phenomenon. Sustainable crop production requires a better understanding of hormesis, its basic concepts, and the input variables to make its management feasible. This implies that acknowledging hormesis in plant research could allow specifying beneficial effects to effectively manage environmental stressors according to cultivation goals. Several factors have been useful in this regard, which at low doses show beneficial eustressing effects (biostimulant/elicitor), while at higher doses, they show distressing toxic effects. These insights highlight biostimulants/elicitors as tools to be included in integrated crop management strategies for reaching sustainability in plant science and agricultural studies. In addition, compelling evidence on the inheritance of elicited traits in plants unfolds the possibility of implementing stressors as a tool in plant breeding.

Hormesis effects in tomato plant growth and photosynthesis due to acephate exposure based on physiology and transcriptomic analysis.

Hormesis is a common phenomenon in toxicology described as low-dose stimulation due to a toxin which causes inhibition at a high dose. Pesticide hormesis in plants has attracted considerable research interest in recent years; however, the specific mechanism has not yet been clarified. Acephate is an organophosphorus insecticide that is used worldwide. Here, hormesis in tomato (Solanum lycopersicum L.) plant growth and photosynthesis after acephate exposure is confirmed, as stimulation occurred at low stress levels, whereas inhibition occurred after exposure to high concentrations. We found that low acephate concentration (5-fold lower than recommended application dosage) could enhance chlorophyll biosynthesis and stimulate photosynthesis effects, and thus improve S. lycopersicum growth. A high level of acephate (5-fold higher than recommended application dosage) stress inhibited chlorophyll accumulation, decreased photosystem II efficiency and blocked antioxidant reactions in leaves, increasing reactive oxygen species levels and damaging plant growth. Transcriptomic analysis and quantitative real-time PCR results revealed that the photosynthesis - antenna proteins pathway played a crucial role in the hormesis effect, and that LHCB7 as well as LHCP from the pathway were the most sensitive to acephate hormesis. Our results showed that acephate could induce hormesis in tomato plant growth and photosynthesis, and that photosystem II and the photosynthesis - antenna proteins pathway played important roles in hormesis. These results provide novel insights into the scientific and safe application of chemical pesticides, and new guidance for investigation into utilizing pesticide hormesis in agriculture. © 2023 Society of Chemical Industry.

Thiamethoxam in soybean seed treatment: Plant bioactivation and hormesis, besides whitefly control?

Amid concerns on the myriad of existing chemical stressors in agroecosystems, pesticides and particularly neonicotinoid insecticides are in the forefront. Despite that, these neurotoxic compounds remain the dominant group of insecticides in worldwide use with the added versatility of use in seed coatings. Such use sparks environmental concerns counterbalanced by their reported insecticidal efficacy and potential plant bioactivation. Nonetheless, this alleged double benefit and interconnection expected with neonicotinoids has been little explored particularly when the whole plant phenology is considered. Regardless of the expected efficacy against targeted insect pest species, like whiteflies, neonicotinoids may spark dual effect on plants – negative at higher concentrations, positive at low concentrations, which is consistent with the hormesis phenomenon that may be expressed as a plant bioactivation. This effect may also cascade to the targeted insect species, what deserves attention. Therefore, soybean seeds treated with increasing concentrations of the neonicotinoid thiamethoxam were followed throughout their development in greenhouse, recording the plant response and yield, besides their effect in whiteflies (Bemisia tabaci MEAM1). Thiamethoxam application was correlated to leaf contents of thiamethoxam and its metabolite clothianidin. Plant hormesis was found for leaf area and root growth, but not for other plant morphological or physiological parameters, nor plant yield. The insecticide concentration-dependency compromised whitefly population growth without evidence of cascading any plant-mediated hormesis to the insects. Thus, although plant hormesis was recognized with thiamethoxam in treated soybean seeds in relevant parameters, no evidence of plant bioactivation was observed to justify its use with such a secondary objective, nor did this hormesis impair whitefly control.

A method for assessing the frequency of hormetic trade-offs in plants

Hormesis is a biphasic response to stress, involving low-dose stimulation and high-dose inhibition. Currently, attempts are being made to use the hormetic stimulation of plant parameters to increase resilience to severe stress. However, due to hormetic trade-offs, low-dose stress does not always cause synchronous improvement of different plant parameters. Some parameters do not change and even some even worsen compared to the control. Therefore, there is a need to evaluate the frequency of hormetic trade-offs. In this study, a method for estimating the probabilities of two types of hormetic trade-off in plants was developed. This method is intended solely to improve the scientific understanding of plant hormesis and a first step towards a more extensive evaluation of hormetic trade-offs in plants.•The proposed method estimates the probabilities of hormetic trade-offs 1 and 2 in plants using simple calculations.•This method can be applied to estimate the probability of hormetic trade-offs 1 and 2 both for a set of independent experiments and in a single experiment.•This method could be used in the future to identify doses and factors that stimulate the greatest number of traits without worsening others.

Atmospheric Pb induced hormesis in the accumulator plant Tillandsia usneoides

While numerous studies reported hormesis in plants exposed to heavy metals, metals were commonly added in the growth substrate (e.g. soil or solution). The potential of heavy metals in the atmosphere to induce hormesis in plants, however, remains unknown. In this study, we exposed the widely-used accumulator plant Tillandsia usneoides to 10 atmospheric Pb concentrations (0–25.6 μg·m−3) for 6 or 12 h. Three types of dose-response relationships between different response endpoints (biomarkers) and Pb concentrations were found for T. usneoides. The first was a monophasic dose response, in which the response increased linearly with increasing Pb concentrations, as seen for metallothionein (MT) content after a 6-h exposure. The second and dominating type was a biphasic-hormetic dose response, exhibited by malondialdehyde (MDA), superoxide anion radical (O2−), and superoxide dismutase (SOD) after 6 or 12 h of exposure and by glutathione (GSH) and MT content after 12 h of treatment. The third type was a triphasic dose response, as seen for leaf electric conductivity after 6 or 12 h of exposure and GSH after 6 h of exposure. This finding suggests that Pb inhibited the response of T. usneoides at very low concentrations, stimulated it at low-to-moderate concentrations, and inhibited it at higher concentrations. Our results demonstrate diverse adaptation mechanisms of plants to stress, in the framework of which alternating between up- and down-regulation of biomarkers is at play when responding to different levels of toxicants. The emergence of the triphasic dose response will further enhance the understanding of time-dependent hormesis.

Induction of hormesis in plants by urban trace metal pollution

Hormesis is a dose–response phenomenon observed in numerous living organisms, caused by low levels of a large number of stressors, among which metal ions. In cities, metal levels are usually below toxicity limits for most plant species, however, it is of primary importance to understand whether urban metal pollution can threaten plant survival, or, conversely, be beneficial by triggering hormesis. The effects of Cd, Cr and Pb urban concentrations were tested in hydroponics on three annual plants, Cardamine hirsuta L., Poa annua L. and Stellaria media (L.) Vill., commonly growing in cities. Results highlighted for the first time that average urban trace metal concentrations do not hinder plant growth but cause instead hormesis, leading to a considerable increase in plant performance (e.g., two to five-fold higher shoot biomass with Cd and Cr). The present findings, show that city habitats are more suitable for plants than previously assumed, and that what is generally considered to be detrimental to plants, such as trace metals, could instead be exactly the plus factor allowing urban plants to thrive.

Environmental hormesis of non-specific and specific adaptive mechanisms in plants

Adaptive responses of plants are important not only for local processes in populations and communities but also for global processes in the biosphere through the primary production of ecosystems. In recent years, the concept of environmental hormesis has been increasingly used to explain the adaptive responses of living organisms, including plants, to low doses of natural factors, both abiotic and biotic, as well as various anthropogenic impacts. However, the issues of whether plant hormesis is similar/different when it is induced by mild stressors having different specific effects and what is the contribution of hormetic stimulation of non-specific and specific adaptive mechanisms in plant resilience to strong stressors (i.e., preconditioning) remains unclear. This paper analyses hormetic stimulation of non-specific and specific adaptive mechanisms in plants and its significance for preconditioning, the phenomenon of the hormetic trade-off for these mechanisms, and the position of hormetic stimulation of non-specific and specific adaptive mechanisms in the system of plant adaptations to environmental challenges. The analysis has shown that both non-specific and specific adaptive mechanisms of plants can be stimulated hormetically by mild stressors and are important for plant preconditioning. Due to limited plant resources, non-specific and specific adaptive mechanisms have hormetic trades-offs 1 (hormesis accompanied by the deterioration of some plant traits) and 2 (hormesis of some plant traits with the invariability of others). At the same time, hormetic trade-off 2 is observed much more often than hormetic trade-off 1, at least, this was demonstrated here for non-specific adaptive responses of plants. The hormetic stimulation of non-specific and specific adaptive mechanisms is part of the inducible adaptation of plants caused by stress factors and is an adaptation to random (unpredictable) changes in the environment.

Uptake of hexavalent chromium by tomato (Solanum lycopersicum L.) plants and mediated effects on their physiology and productivity, along with fruit quality and safety

Cr(VI) is known to induce hormesis in plants. Nevertheless, its effects on the quality of agricultural produce are poorly studied. In the present study, tomato (Solanum lycopersicum L.) plants were exposed to incremental Cr(VI) concentrations through irrigation with water containing K2Cr2O7 in escalating concentrations (0.05, 0.5, 1, 5 and 10 mg L−1), aiming at investigating potential mediated effects on (1) yield, (2) stress responses in leaves, (3) the biosafety of fruits and (4) the mediated alterations in tomato fruit quality attributes. Plants in all Cr(VI) treatments displayed similar phenotype, with no modulations in fruit setting. Plants irrigated with water containing 10 mg L-1 K2Cr2O7 had lower photosynthetic pigment concentration and suffered from oxidative stress in their leaves (evidenced by increased MDA and H2O2 content). Elevated Cr(VI) treatments (5 or 10 mg L-1 K2Cr2O7) resulted in the production of fruits with lower weight and smaller size, along with increased ripening index and soluble solids, lycopene, β-carotene and soluble carbohydrate (fructose, sucrose, total sugars) content. Alterations in fruit quality attributes were at least in part corroborated by the abundance of transcripts related to sucrose (SlSuSys, SlLin5, SlLin6, SlLin7) and carotenoid (SlZDS, SlCRTISO, SlbLCY) biosynthesis. The concentration of total Cr in all fruit samples was far below the maximum permissible limits, thus indicating safety for human consumption. Overall, results presented herein provide novel evidence that Cr(VI) can exert significant effects on the quality of agricultural produce at the biochemical and molecular level, indicating that Cr(VI)-contaminated water should be cautiously used after rigorous investigation.

Glyphosate hormesis mitigates the effect of water deficit in safflower (Carthamus tinctorius L.).

The current climate change scenario may affect water availability in the soil, impacting the agricultural sector. Planting of safflower (Carthamus tinctorius L.) has increased because of its potential for cultivation under drought conditions during the off-season in Brazil and its high potential for use in biofuel production. There are several reports about the potential of low doses of glyphosate to promote plant growth and development (hormesis). Despite the concept of glyphosate hormesis being well established, little is known about any mitigating effect on plants under water deficit conditions. The hypothesis raised is that low doses of glyphosate promote water stress tolerance during the growth and reproductive phases of C. tinctorius exposed to different water regimes. In regimes with and without water deficiency, growth of plants treated with low doses of glyphosate increased, reaching a maximum stimulus amplitude of ~ 131% of control. However, plants under water deficit required lower doses to achieve maximum growth and development. They maintained photosynthetic rates at the level of well-watered plants because they had reduced stomatal conductance and transpiration. Gains in plant height and leaf area were the same as for controls. Low doses of glyphosate can act as mitigators of water deficit in C. tinctorius, allowing plants to maintain their metabolism, reaching levels close to those of plants without water stress, as observed for plant height and leaf area. Our findings indicate that there are even greater implications for understanding glyphosate hormesis in plants under drought conditions, given the current climate change scenario. © 2020 Society of Chemical Industry.

Nanoparticle treatment of maize analyzed through the metatranscriptome: compromised nitrogen cycling, possible phytopathogen selection, and plant hormesis

BackgroundThe beneficial use of nanoparticle silver or nanosilver may be confounded when its potent antimicrobial properties impact non-target members of natural microbiomes such as those present in soil or the plant rhizosphere. Agricultural soils are a likely sink for nanosilver due to its presence in agrochemicals and land-applied biosolids, but a complete assessment of nanosilver’s effects on this environment is lacking because the impact on the natural soil microbiome is not known. In a study assessing the use of nanosilver for phytopathogen control with maize, we analyzed the metatranscriptome of the maize rhizosphere and observed multiple unintended effects of exposure to 100 mg kg−1 nanosilver in soil during a growth period of 117 days.ResultsWe found several unintended effects of nanosilver which could interfere with agricultural systems in the long term. Firstly, the archaea community was negatively impacted with a more than 30% decrease in relative abundance, and as such, their involvement in nitrogen cycling and specifically, nitrification, was compromised. Secondly, certain potentially phytopathogenic fungal groups showed significantly increased abundances, possibly due to the negative effects of nanosilver on bacteria exerting natural biocontrol against these fungi as indicated by negative interactions in a network analysis. Up to 5-fold increases in relative abundance have been observed for certain possibly phytopathogenic fungal genera. Lastly, nanosilver exposure also caused a direct physiological impact on maize as illustrated by increased transcript abundance of aquaporin and phytohormone genes, overall resulting in a stress level with the potential to yield hormetically stimulated plant root growth.ConclusionsThis study indicates the occurrence of significant unintended effects of nanosilver use on corn, which could turn out to be negative to crop productivity and ecosystem health in the long term. We therefore highlight the need to include the microbiome when assessing the risk associated with nano-enabled agriculture.8o2Ktf3WjN9uvowsG6Jw_6Video

Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb.

Hormesis, which describes the stimulatory effect of low doses of toxic substances on growth, is a well-known phenomenon in the plant and animal kingdoms. However, the mechanisms that are involved in this phenomenon are still poorly understood. We performed preliminary studies on corn coleoptile sections, which showed a positive correlation between the stimulation of growth by Cd or Pb and an increase in the auxin and H2O2 content in the coleoptile sections. Subsequently, we grew corn seedlings in hydroponic culture and tested a wide range of Cd or Pb concentrations in order to determine hormetic growth stimulation. In these seedlings the gas exchange and the chlorophyll a fluorescence, as well as the content of chlorophyll, flavonol, auxin and hydrogen peroxide, were measured. We found that during the hormetic stimulation of growth, the response of the photosynthetic apparatus to Cd and Pb differed significantly. While the application of Cd mostly caused a decrease in various photosynthetic parameters, the application of Pb stimulated some of them. Nevertheless, we discovered that the common features of the hormetic stimulation of shoot growth by heavy metals are an increase in the auxin and flavonol content and the maintenance of hydrogen peroxide at the same level as the control plants.

Trace elements-induced phytohormesis: A critical review and mechanistic interpretation

Despite considerable research about biogeochemical behavior of trace elements (TEs) in soil-plant-human systems, there is still a gap of knowledge regarding dose–response relationship, especially for low-applied doses. Trace elements such as mercury, cadmium, antimony and others are highly toxic, without any known essential function in plants. Nevertheless, recent toxicology and risk assessment studies revealed TE-induced hormesis in plants, i.e. stimulation in plant growth at low-doses while suppression at high-doses. This is the first review critically reviewing the TE-induced phytohormetic. The review compares hormetic effects for 366 observations from various research articles among different (i) toxic TEs, (ii) plant species, (iii) plant response parameters (end points), and (iv) exposure durations. It was observed that various toxic TEs, especially Cd, induce hormesis in plants. The mean value of Maximum Stimulatory Response (MAX) was 27% higher compared to the control response, with a range of 0.71 to 1122%. This review critically highlights the TE-induced phytohormesis by discussing possible mechanisms such as the (i) activation of plant tolerance mechanisms after TE-induced overproduction of reactive oxygen species (ROS), and (ii) interplay between phytohormones and TE-mediated ROS production towards plant growth.HighlightsFirst review highlighting trace elements-induced hormesis in plantsTrace elements -induced phytohormesis is evaluated for 366 endpointsThe hormesis varies for different plants, trace elements, endpoints & durationsActivation of plant defense and phytohormones results trace elements-induced hormesisValidation of mechanisms of trace elements-mediated phytohormesis is required

Hormesis of 2,4-D Choline Salt in Biometric Aspects of Cotton

Plant hormesis is a phenomenon that involves the presence of toxic compounds at high doses but can be beneficial at low doses. Thus, the aim of this study was to use dose-response curves to assess the effect of hormesis caused by sub-toxic of 2,4-D choline herbicide on biometric variables of cotton plants. The study adopted a completely randomized experimental design with seven replications, and the treatments consisted of nine fractions of the average rate indicated on the 2,4-D choline salt herbicide label: 0 (control); 0.4275; 0.855; 1.71; 3.42; 8.55; 17.1; 34.2 and 68.4 g ae ha-1 applied at phenological stages V4 or B4 of cotton plants. The plants were assessed for the main morphological parameters. The results were subjected to analysis of variance and, when significant, the hormesis model was tested aiming to describe the dose-response curves with low rates stimulation. The maximum hormesis effect for the variables plant height and number of leaves in cotton plants at stage V4 was estimated, in due order, for sub-doses between 1.14 and 3.02 and 1.37 and 5.54 g ae ha-1 of 2,4-D choline. The hormesis effect was not significant for total dry matter production when 2,4-D choline was applied at stage V4, irrespective of the year of study. Sub-doses between 0.855 and 1.71 g ae ha-1 of 2,4-D choline salt applied at stage B4 caused hormesis in the height, number of leaves, shoots dry matter and total dry matter of cotton plants.

Biphasic effect of abscisic acid on plants: an hormetic viewpoint

Abscisic acid (ABA) is a major phytohormone that regulates development and growth. Increasingly, the literature indicates that ABA enhances and inhibits a spectrum of plant responses within an hormetic-like biphasic dose–response manner. A recent extensive study on the response of a variety of Arabidopsis lines to ABA suggested the widespread occurrence of hormetic dose responses. Our independent reassessment of these data reveals the occurrence of more than 30 hormetic dose–response relationships with quantitative features fully consistent with the substantial and extensively assessed literature on plant hormesis. This analysis significantly extends earlier findings indicating that ABA mediates a broad spectrum of hormetic dose responses in plants. ABA-induced hormesis provides a novel framework for research with the potential to advance the current scientific bases of plant ecology, physiology, and toxicology and to enhance agricultural yields and productivity.

Temperature-induced hormesis in plants

Environmental change attracts particular attention by biologists concerned with the performance of biological systems under stress. To investigate these, dose–response relationships should be clarified. It was previously assumed that the fundamental nature of biological dose–responses follows a linear model, either with no threshold or with a threshold below which no effects are expected to occur in biological endpoints. However, substantial literature, including widespread documentation in plants, has revealed that hormesis commonly occurs. Hormesis is highly generalized and can be utilized as a quantitative measure of biological plasticity. Conditioning induced by adaptive responses also occurs in the framework of hormesis and is of particular importance to environmental change biology with regards to evolutionary adaptations. This paper presents additional evidence for hormetic dose responses induced by temperature in plants. The current understanding on hormesis provides a perspective for next generation environmental change research. Hormesis should have a central role in environmental change biology of vegetation.

Human and veterinary antibiotics induce hormesis in plants: Scientific and regulatory issues and an environmental perspective

Veterinary and human pharmaceuticals have been widely used in the developed world, thus increasing their accumulation in the environment and thereby posing ecological risks. Earlier studies report that active pharmaceutical ingredients induce hormesis in plants, i.e. at low doses may enhance plant health whereas at high doses may suppress plant vigor. There is hitherto no study critically reviewing the effects of antibiotics on plants within a hormetic context despite effects of low doses on plants can have implications to animals, including humans, and to ecological processes. This study critically reviews for first time antibiotic-induced hormesis in plants, both quantitatively and qualitatively. Hormesis was induced by several antibiotics in a variety of species and endpoints. The maximum stimulatory response (MAX) was commonly <1.5-fold the control response and the distance from MAX to no-observed-adverse-effect level (NOAEL) was commonly up to 10–fold. Further quantitative and qualitative evaluations are provided and discussed in relation to scientific and regulatory aspects. Low doses of antibiotics are equally important as high doses as they can negatively affect plants, depending on plant tissues and the time tissues are subject to exposure. Antibiotic-induced hormesis in plants provides a significant environmental perspective and should be incorporated into the hazard and risk assessment process. CapsuleCommon antibiotics released in the environment induce hormesis in plants, urging for re-examination of the risk assessment practices by worldwide regulatory agencies.