Hookworm Eggs Research Articles

The challenge of triple intestinal parasite infections in immigrants-A call for comprehensive differential diagnosis.

Screening for parasitic infections among displaced populations including migrants, refugees, and internally displaced persons is crucial to prevent long-term complications associated with untreated infections. Additionally, prompt treatment with appropriate medications like praziquantel and albendazole can lead to significant improvement and successful patient outcomes. This case highlights the necessity of heightened awareness, early detection, and effective management of parasitic infections to enhance public health efforts and minimize the spread of these diseases. Implementing multisectoral One Health will improve human, animal, and environmental health globally using limited resources. Parasitic infections including Schistosoma spp., hookworm, and Strongyloides spp. are commonly co-existing diseases in underdeveloped countries including South Sudan and Sudan. These infections are often presented with no specific symptoms, therefore they remain undetected and eventually leading to long-term complications. Latent parasitic infections among adults particularly displaced population like migrants and refugees have recently gained global attention. In this case report, we present the case of a young male immigrant from South Sudan with multi-parasitic infections. The patient was admitted to a hospital complaining of gastrointestinal symptoms of hematemesis and melena, which were accompanied by signs of liver and spleen enlargement, coupled with low blood cell counts. While liver function tests showed no abnormalities, stool examinations confirmed the presence of S. mansoni, hook worm eggs, and Rhabditiform first-stage larvae of S. stercoralis. The patient was treated with medications such as praziquantel and albendazole, which resulted in full recovery and discharge from the hospital. This case highlights the importance of considering multiple infections among patients with gastrointestinal symptoms, especially in endemic areas. Cost-effective prevention and control strategy require the implementation of a multisectoral One Health strategy for the surveillance, prevention, and control of parasitic infections in human, animals, and the environment.

A pilot study on the epidemiology, diagnosis and characterization of Echinococcus granulosus sensu lato in sheep, goats and dogs in Siirt province of Türkiye revealed remarkable adaptation of Echinococcus canadensis (G6/G7) in goats

Cystic echinococcosis (CE), caused by Echinococcus granulosus sensu lato (s.l.), represents one of the most significant zoonotic diseases globally, affecting both humans and animals. The objective of this study was to ascertain the prevalence of E. granulosus sensu lato in sheep and goats in a pilot region with a one-year slaughterhouse follow-up period and to determine the genetic differences and haplotypes among sheep, goat, and dog isolates. To this end, the prevalence of CE cysts was determined by monitoring the slaughter of sheep and goats at least three days a week at a slaughterhouse in the Siirt province of Türkiye during 2023. Additionally, faecal samples were collected from stray dogs and analysed using both flotation and molecular techniques. The presence of CE cysts was identified in 569 (11.12 %) of the 5119 sheep and 66 (2.31 %) of the 2860 goats after slaughtering. The highest positivity was observed in November (20.39 %), while the lowest was recorded in July (5.62 %). Of the sheep that detected positive, 25 (4.39 %) were less than one year old, while 544 (95.61 %) were older than one year. Of the infected sheep, 26 (4.57 %) were male and 543 (95.43 %) were female. 204 (35.85 %) sheep exhibited fluid-filled CE cysts, 338 (59.40 %) displayed calcification, and 27 (4.75 %) demonstrated the presence of newly developed cysts. The highest positivity was observed in December (5.83 %), while the lowest was recorded in May (0.62 %) in goats. Of the positive goats, two (3 %) were less than one year old, while the remaining 64 (97 %) were older than one year. Of the goats infected with CE cysts, 10 (15.15 %) were male and 56 (84.85 %) were female. Of the cysts, 56.1 % were fluid-filled, 42.4 % were calcified and 1.5 % were newly developed. Following DNA sequence analysis of CE cyst isolates obtained from the slaughterhouse, all 61 sheep sequences were identified as E. granulosus s.s. (G1/G3). Of the 13 goat isolates, seven were identified as E. granulosus s.s. (G1/G3), while the remaining six were classified as E. canadensis (G6/G7). The centrifugal flotation method was employed to detect the presence of Isospora spp. oocysts in eight dogs, Toxocara canis and hookworm eggs in three dogs each, and Dipyllidium caninum eggs in one dog. A total of 54 dog faeces were examined. No Taeniid eggs were observed in any of the dogs. Following PCR analysis of the mt-CO1 gene region in the dog faecal samples, four samples were positive for a 875 bp band. Only one of these bands was suitable for sequence analysis, which confirmed it as E. granulosus s.s. (G1/G3).

Efficacy and safety of emodepside compared with albendazole in adolescents and adults with hookworm infection in Pemba Island, Tanzania: a double-blind, superiority, phase 2b, randomised controlled trial

Human hookworm is a cause of enormous global morbidity. Current treatments have insufficient efficacy and their extensive and indiscriminate distribution could also result in drug resistance. Therefore, we tested the efficacy and safety of emodepside, a strong anthelmintic candidate that is currently undergoing clinical development for onchocerciasis and soil-transmitted helminth infections. We conducted a double-blind, superiority, phase 2b, randomised controlled clinical trial comparing emodepside and albendazole. Participants in the emodepside group received six 5 mg tablets of emodepside (totalling 30 mg) and one placebo; participants in the albendazole group received one 400 mg tablet of albendazole and six placebos. Participants were recruited from four endemic villages and three secondary schools in Pemba Island, Tanzania. Participants aged 12-60 years were eligible for treatment if they were positive for hookworm infection, and they had 48 or more eggs per gram from four Kato-Katz thick smears and at least two slides had more than one hookworm egg present. Participants' treatment allocation was stratified by infection intensity and efficacy was measured by cure rate: participants who were hookworm positive and became hookworm negative after treatment. Adverse events were reported at 3 h, 24 h, 48 h, and 14-21 days post-treatment. The trial is registered at ClinicalTrials.gov, NCT05538767. From Sept 15 to Nov 8, 2022, and from Feb 15 to March 15, 2023, 1609 individuals were screened for hookworm. Of these, 293 individuals were treated: 147 with albendazole and 146 with emodepside. Emodepside demonstrated superiority, with an observed cure rate against hookworm of 96·6%, which was significantly higher compared with albendazole (cure rate 81·2%, odds ratio 0·14, 95% CI 0·04-0·35; p=0·0001). The most common adverse event in the emodepside treatment group was vision blur at 3 h after treatment (57 [39%] of 146). Other common adverse events were vision blur at 24 h after treatment (55 [38%]), and headache and dizziness at 3 h after treatment (55 [38%] for headache and 43 [30%] for dizziness). In the emodepside treatment group, 298 (93%) of the 319 adverse events were mild. The most commonly reported adverse events in the albendazole treatment group were headache and dizziness at 3 h after treatment (27 [18%] of 147 for headache and 14 [10%] for dizziness). No serious adverse events were reported. This phase 2b clinical trial confirms the high efficacy of emodepside against hookworm infections, solidifying emodepside as a promising anthelmintic candidate. However, although the observed safety events were generally mild in severity, considerations must be made to balance the strong efficacy outcomes with the increased frequency of adverse events compared with albendazole. European Research Council.

Comparison of different molecular protocols for the detection of Uncinaria stenocephala infection in dogs

The present study aims to assess the performance of different molecular targets using various matrices of samples for the detection of Uncinaria stenocephala (US) in hookworm infected dogs. To this end, the DNA extraction was performed on the following matrices of samples: (i) larvae of US obtained from experimentally infected dogs with US with different larvae counts per microliter (µl); (ii) pure US eggs suspension in distilled water with different egg counts per µl; (iii) spiked dog fecal samples with different US eggs per gram (EPG) of feces; (iv) feces from dogs naturally infected with hookworm eggs; (v) fecal suspension with hookworm eggs recovered from the FLOTAC apparatus. All the samples were tested with four different PCR protocols targeting specific regions for the detection of both hookworms US and AC as follows: Protocol A (ITS1, 5.8 S, ITS2) and Protocol B (18 S) for the detection of both species, Protocol C (ITS1) for the detection of AC and Protocol D (ITS1) for the detection of US. The best results were obtained with DNA extracted from US larvae matrix obtained from experimentally infected dogs, showing a detection limit of 3.5 larvae/ml for the protocols A, B and D. A moderate correlation was found between the FLOTAC technique and PCR protocols B and D with respect to fecal samples from dogs naturally infected with hookworms. Indeed, PCR protocols B (18 S) and D (ITS1) gave the best results for feces and fecal suspension from naturally infected dogs. However, all the PCR protocols used showed lower sensitivity than FLOTAC technique. Perhaps, isolating US eggs in advance could help to obtain better quality and quantity of DNA, avoiding some notable factors such as inhibitors present in faecal samples. However, a further study is needed to evaluate and standardise a protocol for the recovery of parasitic elements, that could be applied prior to DNA extraction. Therefore, this could lead to a better amplification of US eggs DNA. In conclusion, our results showed that the type of sample (sample-matrix) used for the DNA extraction samples is crucial, as this affects the diagnostic sensitivity of the technique.

Contribution of wastewater irrigated vegetables to the prevalence of soil-transmitted helminth infection among female farmers in Addis Ababa, Ethiopia

BackgroundUntreated or inadequately treated wastewater carrying human feces can host helminth eggs and larvae, contaminating the soil and plants that are irrigated with it. In Addis Ababa, farmers use untreated wastewater to grow vegetables; however, there are little data currently available published on vegetables' contribution to the prevalence of helminth among female farmers along the Akaki River, in Addis Ababa, Ethiopia.MethodsA cross-sectional study was conducted in Addis Ababa City in February 2022. A stratified random sampling method was used to sample farming households. The sample size for each district was determined by a proportional allocation to the total number of households in the area. Two hundred and fifty-two composite vegetable samples and 101 farmers’ stool samples were collected and analyzed for helminth prevalence. Data on socio-demographics were collected by trained data collators using a structured questionnaire. Kato-Katz concentration was used to detect STH from a stool sample. Stata version 14.0 was used to process the data. Poisson regression was used to identify the association between STH prevalence in the vegetable and the farm's stool.ResultsHelminths were found in 67.5% of vegetables sampled and 20.8% of female farmers' stools. Ascaris lumbricoides eggs (vegetable 48.4% and stool 9.9%) were identified in all analyzed samples. Hookworm eggs (vegetable 13.1% and stool 8.9%) and Trichuris trichiura eggs (vegetable 5.9% and stool 2%) were also isolated. The total number of helminth eggs present in wastewater-irrigated vegetables and female farmers’ stool had a positive association (p < 0.05) with a regression coefficient of 1.92 (95% CI = 1.56–2.28).ConclusionsThe study found a significant prevalence of helminth infections, particularly Ascaris lumbricoides, in stool and vegetable samples irrigated with wastewater. A clear association was found between vegetable production and a higher prevalence of helminth infections among female farmers. Therefore, it is important to ensure that farmers are educated in the importance of food washing and sanitation/hygiene practices when using wastewater irrigation for vegetable crops.Graphical

Penyuluhan dan Pemeriksaan Feses Pada Masyarakat Desa Bone Kecamatan Nekamese Sebagai Upaya Pencegahan Penyakit Tropis

Hookworm infection remains a significant health concern, particularly in rural areas like Bone Village, Nekamese District. This community service project aimed to conduct health education and stool examinations to prevent and control tropical diseases. Stool examinations were performed on 60 samples from the Bone Village community, revealing that 2 samples tested positive for hookworm eggs, indicating the presence of hookworm infection, although the prevalence was relatively low. Following this, an interactive health education session was conducted on March 24, 2024, at the village church, involving the local community. The educational materials covered the prevention and treatment of hookworm infection, the importance of personal and environmental hygiene, and how to detect early symptoms of tropical diseases. This activity successfully raised community awareness about the importance of preventing tropical diseases and was published in the online media Kupang, reaching a broader audience. The results of the community service project demonstrated that a participatory and educational approach is effective in reducing the risk of hookworm infection and improving overall community health

Familial aggregation of human hookworm infections in Sichuan Province

To investigate the family aggregation of human hookworm infections in Sichuan Province and to identify its influencing factors, so as to provide insights into management of hookworm infections. Three to four counties (districts) were sampled from basins, hilly regions and mountainous regions around the basins in Sichuan Province from 2017 to 2022 as fixed survey sites, and 17 to 30 counties (districts) were selected as mobile survey sites. At least 1 000 permanent residents at ages of 3 years and older were sampled from each survey site, and hookworm eggs were detected in human stool samples using the Kato-Katz technique. Subjects with 2 and more family members and at least 2 individuals diagnosed with hookworm infections in the county (district) where they lived were selected, and the familial aggregation of hookworm infections was analyzed using the test of goodness of fit for binomial distribution. In addition, the knowledge and practice of hookworm disease control were investigated among residents in Hejiang County and Wutongqiao District, Leshan City, Sichuan Province in 2021 and 2022, and the difference in the knowledge and practice of hookworm disease control was compared between members with and without familial aggregation of hookworm infections. A total of 66 812 residents from 25 196 households were sampled from main endemic areas of hookworm diseases in Sichuan Province from 2017 to 2022 for detection of hookworm infections, and 4 403 infections were identified (6.59% prevalence). The distribution of human hookworm infections in Sichuan Province did not fit the binomial distribution, and showed family aggregations (χ2 = 2 116.759, P < 0.001). Family aggregation of human hookworm infections was found in endemic areas with 1% and higher prevalence of human hookworm infections (χ2 = 136.006 to 428.738, all P values < 0.001), and family aggregation of human hookworm infections was identified in different years (χ2 = 87.615 to 471.838, all P values < 0.001) and in different terrains of endemic areas (χ2 = 8.423 to 1 144.176, all P values < 0.001). The members with hookworm infections had median eggs per gram of 180 (interquartile range, 780) in aggregated families and 72 (102) in non-aggregated families (Z = -2.686, P < 0.05). The proportion of members in families with aggregation of hookworm infections who knew the preventive measures of hookworm disease was significantly lower than in non-aggregated families (24.49% vs. 51.72%; χ2 = 10.262, P < 0.05), and the proportion of members in families with aggregation of hookworm infections who often worked barefoot on the ground was significantly higher than in non-aggregated families (30.61% vs. 13.25%; χ2 = 6.289, P < 0.05). There is a familial aggregation of human hookworm infections in Sichuan Province, and awareness of preventive measures for hookworm disease and frequent working barefoot on the ground are associated with familial aggregation of hookworm infections.

Assessment of Intestinal Parasitic Infection and Personal Hygiene Practice Among School Children in A Primary School in A Village in Jakarta, Indonesia

Background: Intestinal parasitic infections (IPI) are still among developing countries' most prevalent parasitic infections. IPI is closely linked to nutrition, personal hygiene, environmental sanitation, income, climate, and education. Aims/Objectives: To assess the IPI and personal hygiene status among school children in the flood-prone area in Jakarta, Indonesia. Methodology: A cross-sectional study was conducted in a government primary school in South Jakarta, Indonesia, involving 157 students. A structured questionnaire was developed to assess the personal hygiene behavior of the students. A physical examination of the nail fingers was conducted as well. Feces samples were collected from each student and examined using direct smear techniques to identify intestinal parasite infection. Results: The prevalence of IPI was 38.2% in single and mixed infections. Intestinal parasites recorded in this study were Blastocystis hominis, Giardia lamblia, Trichuris trichiura, hookworm eggs, and non-pathogen protozoa Entamoeba coli. There is no significant difference between the prevalence of IPI with footwear usage, nail-cutting once a week (p=0.718), handwashing before the meal (p=0.688), handwashing after defecations (p=0.618); however, there is a significant difference between nail cleanliness and IPI (p=0.003). Conclusions: The prevalence of IPI in these school children was high and required interventions like health education and action on nail and hand hygiene behavior from school personnel or teachers.

Prevalence of Cockroach Density, Gastrointestinal Disorders, and Identification of Parasites in Cockroaches in Helvetia Deli Serdang Village

Background. Cockroaches are known to be vectors of various pathogens that can infect humans, one of which is intestinal parasites. Parasitic infectious diseases can cause symptoms of gastrointestinal disorders in sufferers, which can interfere with the sufferer's quality of life. This research aims to determine cockroach density, and gastrointestinal disorders in the community, and identify parasites in cockroaches. Method. The research method used in this research is observational analytics. The population and sample for this study were the houses of the Helvetia Deli Serdang Village community which met the slum criteria of 100 houses. The caught cockroaches are then examined in the laboratory. Results. The results obtained were that 96 houses (96%) were not infested with cockroaches, 4 houses (4%) had no cockroaches, and no houses were infested with cockroaches. The number of respondents who had experienced gastrointestinal disorders was 41 respondents (41%) and 59 respondents (59%) had never experienced them. Based on laboratory examination, 22 rhabditiform hookworm larvae (21.78%), 5 hookworm eggs (4.95%), and 1 Oxyruris vermicularis egg (0.99%) were found. Toxocara sp. as many as 1 piece (0.99%), and cysts Entamoeba histolytica as much as 1 piece (0.99%). Conclusion. Based on these results, there was no high density of cockroaches found at the research location, the majority of respondents had never experienced gastrointestinal disorders in the past year, and parasites were found in cockroaches.

Intestinal Helminth Infections and Their Association with QuantiFERON-TB Gold Plus Test Performance in an Endemic Setting, Northwest Ethiopia.

Timely detection and treatment of latent TB infection (LTBI) is part of WHO's strategy against tuberculosis (TB). Helminth infections can modulate immune responses, potentially impacting the performance of interferon-gamma release assays (IGRAs) such as the QuantiFERON-TB Gold Plus (QFT-Plus). This study evaluated the association between helminth infections and QFT-Plus results among participants from a TB-endemic region. A cross-sectional study was conducted from October 2022 to March 2023 in Bahir Dar, Ethiopia. Stool samples of 314 potential participants were examined for helminths using wet mount and Kato-Katz techniques. LTBI was assessed by QFT-Plus from a total of 100 gender-matched helminth-positive and -negative participants. The association between helminth infection status, egg count, and QFT-Plus positivity was analyzed, and p values <0.05 were considered significant. Overall, 53 of 314 screened participants were infected with helminths (16.9%), with A. lumbricoides (47.2%) and hookworm (30.2%) as most prevalent species. The overall QFT-Plus positivity rate was 30.0%, with similar rates observed between helminth-positive and helminth-negative participants. Although QFT-Plus positivity was slightly lower in hookworm carriers (25%) compared to those with A. lumbricoides (32%), a higher-than-median hookworm egg burden was significantly associated with reduced QFT-Plus positivity (P = 0.029). QFT-Plus positivity was significantly higher among male participants than females (P = 0.032). While overall helminth infection status did not significantly affect QFT-Plus positivity, higher hookworm burden was associated with reduced QFT-Plus reactivity. These findings suggest that the type of helminth and infection intensity, rather than its mere presence, may influence IGRA performance. Further studies with larger sample sizes are warranted to understand the species-specific effect of helminth infection on immune modulation of the host.

Soil-Transmitted Parasites and Non-Pathogenic Nematodes in Different Regions of Porto Alegre City, Brazil: A Comparison between Winter and Summer

We assessed the prevalence of soil-associated parasites and non-pathogenic nematodes in eight public areas of Porto Alegre (Rio Grande do Sul state, southern Brazil), the most populous city in Rio Grande do Sul. Soil samplings were carried out during the winter of 2022 and summer of 2023: A total of 80 samples were collected in winter and 80 in summer (ten samples from each sampling site per season), totaling 160 soil samples. The frequency of microscopic non-pathogenic nematode larvae was significantly higher (p = 0.048) in winter (93.75%) than in summer (82.50%). Considering the pooled data from winter and summer (n = 160) for human pathogenic parasites, the following frequencies were observed (using microscopy analysis): hookworm (filariform) larvae (1.25%), hookworm (rhabditiform) larvae (11.25%), Strongyloides spp. (filariform) larvae (0.63%), Strongyloides spp. (rhabditiform) larvae (2.5%), hookworm eggs (10.63%), Ascaris spp. eggs (10.00%), and Trichuris spp. eggs (1.25%). Hookworm (rhabditiform) larvae were the most frequent parasitic structures (15.00%) in winter, and A. lumbricoides eggs were the most frequent parasitic structures (8.75%) in summer. No statistically significant difference was observed in the frequency of pathogenic parasites between the seasons (p &gt; 0.05). Toxoplasma gondii DNA was assessed, but all soil samples tested negative in molecular analysis. Our results indicate that soil from many regions of Porto Alegre shows a high prevalence of soil-transmitted helminths, indicating the need for improvements in social conditions and environmental sanitation in the city. Our study also suggests that climate change may affect soil biodiversity, potentially harming non-pathogenic nematodes and favoring human pathogenic parasites.

Distribution of helminth eggs in environmental and stool samples of farming households along Akaki River in Addis Ababa, Ethiopia

BackgroundHelminth infections are a public health issue in countries with poor sanitation facilities. However, there little information on the epidemiological association between helminths in wastewater and soil samples and rates of helminth infection among farming households along the Akaki River in Addis Ababa, Ethiopia.MethodsA cross-sectional study was conducted between November 2021 and February 2022. A stratified random sampling technique was used to select farming households. The sample size for each district was determined by a proportionate allocation to the number of households. From wastewater-irrigated farms, 70 wastewater samples, 28 soil samples, and 86 farmers' stool samples were collected and analyzed for helminths. A questionnaire was used to gather ethnographic data, about farming households, whereas wastewater and soil sample analysis was used to generate quantitative data on helminth loads. The data were systematically analysed by developing themes, and bias evaluated using triangulation validation methodologies. Potential pathways to helminth infection were evaluated by measuring. Total number of helminth eggs in wastewater, soil samples and farmer's stools was investigated using Poisson regression.ResultsIn this study, 82.9% of wastewater samples, 57.1% of soil samples, and 18.6% of farmers' stool samples contained helminth eggs. The most prevalent helminth was Ascaris lumbricoides in all samples (wastewater 67%, soil 25%, and stool 10.5%), followed by hookworm (wastewater 10%, soil 21.4%, and stool 6.9%) and Trichuris trichiura eggs (wastewater 5.7%, soil 10.7%, and stool 1.2%). There was a positive association between the total number of helminth eggs in wastewater and soil samples with counts in farmers’ stool. The Poisson regression coefficients for wastewater and soil were, 1.63 (95% CI = 1.34–1.92) and 1.70 (95% CI = 1.39–2.01), (p < 0.05).ConclusionsThis research has shown a clear association between the total helminth eggs in wastewater and soil samples and farmer stools along the Akaki River. Therefore, an integrated approach is essential to address the issue in this area and prevent the spread of further helminth infections.

Le parassitosi intestinali

Parasitic infections are still a major public health problem in developing countries. The paper presents the case of an 18-month-old girl taken by her mother to the paediatrics department of St. Luke’s Hospital in Wolisso, Ethiopia for general malaise, fever, asthenia and pallor for several days. On suspicion of anaemia, a hemochromocytometric examination was carried out, which showed severe microcytic anaemia, so it was decided to proceed with a whole blood transfusion, after a smear test for malaria parasites. Following a negative test for malaria, it was decided to proceed with a fresh microscopic examination of the faeces, which proved positive for hookworm eggs. Therapy with albendazole was then prescribed, at a dose of 200 mg per os as a single dose, with improvement in the clinical picture. This case underlines the importance of considering hookworm infection as a possible cause of severe anaemia, especially in areas where malaria is not endemic. It is essential to engage in hookworm infection control through preventive measures, such as hygiene, anti-parasite therapy and health education, to reduce the burden of disease in populations at risk. Moreover, a summary review of the main intestinal parasites is reported.

The Influence of Inadequate Handling Conditions on the Prevalence of Parasites in Commonly Consumed Fluted Pumpkin Leaves (&lt;i&gt;Telfairia occidentalis&lt;/i&gt;) in Delta State, Nigeria

Vegetables are crucial for a healthy diet; however, when not washed properly, they can act as carriers of protozoa cysts and helminth eggs/larvae, posing a risk of transmission. The study aimed to determine the prevalence of parasitic contamination of fluted pumpkin promoted by inadequate handling conditions in Delta State, Nigeria. Samples of Telfairia occidentalis (fluted pumpkin vegetables) were collected from Effurun town and Otokutu local markets in washed and unwashed conditions and examined for parasitic contamination. An interviewer-administered questionnaire was used to obtain information on the socio-economic factors associated with parasitic contaminations on vegetables. The vegetable samples were examined using the simple sedimentation concentration method. The educational status of the vendors was ascertained, and 75% of the vendors had secondary school education, while 25% had primary school education. Four hundred seventyeight parasitic contaminants were recovered from the 48 vegetables samples. The species and stages of parasites detected were Trichuris spp, Moniezia spp, Enterobius vermicularis, Ascaris lumbricoides, Strongyloides spp, Hookworm eggs, Strongyle eggs Strongyloides stercoralis larvae, Schistostoma spp, Platynosomum fastosum and Eimeria spp. Fifty per cent (50%) of the produce was not washed, while 50% of the produce was washed before displaying by the retailers. The result from the paired t-test revealed a significant difference (p&lt;0.05) in total parasitic contamination between washed and unwashed vegetable samples from both Effurun and Otokutu markets. This study showed that handling conditions of washing and the environment where vegetables are sold can influence the amount of parasitic load. Therefore, adequate washing and proper handling of raw edible vegetables before consumption are recommended. The government must create awareness and educate vegetable vendors about the importance of proper handling and hygiene, as they can play a major role in mitigating public health issues.

Assessment of the capacity of Whatman filter papers as support to store stools for the molecular diagnostic testing of soil-transmitted helminthiasis

Storage of stools for the detection of soil-transmitted helminths (STH) remains challenging for the molecular diagnostic testing of STH infections. This study aimed to overcome this challenge by assessing the capacity of Whatman filter papers to store stools for the molecular detection of STHs. Stool samples were collected from school-aged children of soil-transmitted helminthiasis endemic areas of Cameroon and then, analysed using Kato Katz technique. For this study, 128 and 40 stool samples respectively with and without STH eggs were analysed. From each sample, 10, 20, 40 and 80 mg of stool were weighted and spread on 6 grades of Whatman filter papers that were stored at room temperature from one to ten weeks. DNA was extracted from spread stool using CTAB based-method. The amount of stool to spread on filter papers and the grade of filter paper offering good storage were determined by amplifying specific DNA fragments of Ascaris lumbricoides. The capacity of filter papers to store stool samples for several weeks before the molecular detection of STH species was assessed by amplifying specific DNA fragments of different STHs. The amplification rates of A. lumbricoides were significantly higher (P < 0.0001) for 10 and 20 mg of stored stools. Stools spread on Whatman paper grade 2 yielded the highest amplification rate of 100% for A. lumbricoides, T. trichiura and hookworm. PCR revealed STH infections in all the 128 spread stools carrying STH eggs. It also revealed Necator americanus and Ancylostoma duodenale respectively in 10 and 13 of 15 spread stools contained hookworm eggs. PCR confirmed the co-infections of these hookworm species as well as that of A. lumbricoides and Trichuris trichiura in 7 spread stools. Out of 40 stools without STH eggs, PCR revealed that 5 (12.5%) and 9 (22.5%) had respectively A. lumbricoides and T. trichiura infections. The amplification rate of each STH species was 100% from one to 8 weeks and decreased to 86.7% after 10 weeks of storage. This study highlighted the capacity of filter papers to store stools for the molecular detection of STHs. Storing stools on these papers will enable to monitor and evaluate control programs and ensure post-elimination surveillance.

Bougainvillea Flower Extract Utilization as Eosin Substitute in Worm Eggs Microscopic Observation

Introduction: Flavonoids, glycosides, alkaloids, phlobatannins, saponins, steroids, tannins, and terpenoids are all known components of bougainvillea flowers. The betalain compounds betasianin and betaxanthin, which give bougainvillea flowers their color, are present. Betalains are water-soluble pigments classified into betacyanins (red) and betaxanthins (yellow). The eosin dye, which can stain worm eggs, is comparable to the pigment in bougainvillea flowers. Because reagents are expensive, raw materials from the local area must be used instead. Local raw materials can replace eosin reagents. When examining intestinal nematode worm eggs, the extract of bougainvillea flowers can be used as an eosin reagent. We are interested in learning whether Bougenvillea flower extract can be used to observe worm eggs at a microscopic level instead of the expensive and difficult-to-come-by eosin. Aim: This study aimed to ascertain whether Bougainvillea extract could be used instead of eosin to observe worm eggs at a microscopic level. Method: Complete Randomised Design (CRD) is used in this quasi-experimental research. This study also aims to compare the efficacy of eosin reagent and bougainvillea flower extract in the protracted drying process. The Microbiology Laboratory, Department of Medical Laboratory Technology, Aceh Health Polytechnic, Ministry of Health, was the site of this study. Gampong Jawa, Banda Aceh, is where feces were sampled. Preparation of bougainvillea flower extract with a concentration of 100% as a substitute for 2% eosin on microscopic observations of Ascaris lumbricoides, Trichuris trichiura, and Hookworm eggs to see the comparison of the drying time for reagents in worm egg preparations using red, pink and orange Bougainvillea flower extract with eosin control. Repeat three more times. T-test used for data analysis. Result: When compared to the eosin control reagent, which dried in only 27.63 minutes in preparation for Soil Transmitted Helminth worm eggs, the average drying time for the reagents using natural reagents from the extract of red bougainvillea flowers was 23.96 minutes, that of pink bougainvillea flowers was 23.30 minutes, and that of orange bougainvillea flowers was longer at 28.40 minutes. A variety of bougainvillea species have different drying requirements for regeneration. Conclusion: The time it took for red, pink, and orange Bougainvillea extract to dry, as well as the control eosin, indicates that there is a significant difference in the dry times between the treatments. Keywords: Bougainvillaea extract, eosin reagents, worm eggs

Multiple anthelmintic drug resistant Ancylostoma caninum in foxhounds

Ancylostoma caninum is the most common and important gastrointestinal nematode of dogs in the United States. Despite recent reports of A. caninum isolates resistant to all classes of anthelmintics, little is known about the frequency and extent of this anthelmintic resistance. The study aim was to evaluate the efficacy of three commercial anthelmintic products in the treatment of foxhound dogs with a history of persistent A. caninum infections. In the first phase of this study, 35 foxhounds were randomly divided into three treatment groups: moxidectin/imidacloprid (MI), pyrantel pamoate/febantel/praziquantel (PFP), and emodepside/praziquantel (EP). Fecal samples were collected on day 0, 11, and 33 post-treatment (PT), and hookworm eggs were quantified using the mini-FLOTAC technique with a multiplication factor of 5 eggs per gram (EPG). The fecal egg count reduction (FECR) on day 11 PT was 65% (95% CI: 62%–68%) for MI, 69% (95% CI: 66%–72%) for PFP, and 96% (95% CI: 94%–97%) for EP. On day 33 PT, the FEC in the MI and PFP groups returned to almost the same values as on day 0, while in the EP group, the FEC remained low. Since MI and PFP proved ineffective, 32 animals were randomly divided into two groups in the second phase. They were treated either with a combination of MI/PFP or EP. The FECR at day 13 PT for the combination MI/PFP was 89% (95% CI: 87%–91%) and 99% (95% CI: 98%–99%) for EP. These results suggest that this A. caninum population is resistant to multiple anthelmintics. Although the combination of MI/PFP improved the anthelmintic efficacy, the FECR remained below 90%. Future studies are indicated to evaluate further the epidemiology of persistent hookworm infections in dogs in the US and to identify more effective treatment protocols as they pose a significant health risk to canine and human health.

Description of Soil Transmitted Helminths (STH) Worms Infection in Coconut Farmers in Guntung Village, Batu Bara District, North Sumatra, Indonesia

Abstract: Worms or often calledSoil Transmitted Helminths (STH) is a disease of the human body caused by intestinal nematode worm infection. One of the transmission of this worm is generally through soil media. STH species include roundworms, whips, mines. Farmers as one of the professions in the community have a great risk of being infected with STH, which is more than 70%. The majority of agricultural land in Guntung Village supports working as a farmer. Factors that cause helminth infections are related to poor environmental hygiene and unhealthy lifestyles. This study aims to determine the description of infection with Soil Transmitted Helminths (STH) infarmer coconut inGuntung Village, Batu Bara District. This study uses observational research methods (non-experimental) with descriptive research criteria. The number of samples is 50 faeces fromfarmer in Guntung Village, Batu Bara District. The results of the stool examination by the flotation method found that 12 respondents (24%) were positively infected with STH, namely hookworm eggs. The conclusion of this study is the possibility of farmers who are positive because they come from other environments, not from agricultural land and soil in their home environment. Therefore suggestions for the community or farmers can increase knowledge about the transmission and prevention of STH infections, increase awareness to carepersonal hygiene as well as sanitation of the surrounding environment and being able to take deworming medication for treatment of STH-infected farmers.

Associations between canine hookworm infection and dog owners' awareness, perception, and behaviour: A cross-sectional study in Brisbane, Queensland, 2019-2020.

Canine hookworms are found globally, and infections have been recorded in domestic dogs visiting dog parks in Queensland, Australia. Some canine hookworms also present a risk of zoonotic transmission to humans. Potential transmission of hookworms can occur in the household and at public places because of lack of owner awareness and poor coverage of canine deworming. Between April 2019 and March 2020, faecal samples from owned dogs were collected from 39 dog parks in metropolitan Brisbane, Queensland, and tested for the presence of hookworm eggs using faecal floatation technique. Dog owners who provided samples were requested to complete a survey on their awareness, perceptions and behaviour regarding the risks and consequences of canine parasitic infection. Associations between dog owner demographics and responses to the survey were measured using Goodman and Kruskal's gamma. Statistical associations between canine hookworm infection in dogs and their owner's responses were quantified using a two-sample Wilcoxon rank-sum test. A total of five canine faecal samples from 175 respondents were found positive for hookworm eggs, corresponding to a sample- and park-level prevalence of 2.86% and 12.82%, respectively. Female dog owners were found to be more aware of the importance of regular deworming of their dogs (|γ| = 0.405). Our results indicate that hookworm infection was associated with a lower awareness of the importance of deworming (p = 0.007), less diligence in administration of deworming (p = 0.004), lower awareness of the risk of acquiring parasites from raw meat (p = 0.010), less likelihood of cooking meat before feeding it to their dogs (p = 0.028), and less likelihood to properly dispose their dog's faeces (p = 0.027). This study not only indicates a need for improving owner education towards the importance of deworming but also a need for changing owner's behaviours to reduce potential environmental contamination with infective hookworms. The latter in particular indicates a broader public health risk of disease transmission in public places due to improper disposal of dog faeces, especially in areas with higher canine hookworm infections.

Emodepside for Trichuris trichiura and Hookworm Infection

BackgroundCurrent treatments for soil-transmitted helminth infections in humans have low efficacy against Trichuris trichiura. Emodepside — a drug in veterinary use and under development for the treatment of onchocerciasis in humans — is a leading therapeutic candidate for soil-transmitted helminth infection.MethodsWe conducted two phase 2a, dose-ranging, randomized, controlled trials to evaluate the efficacy and safety of emodepside against T. trichiura and hookworm infections. We randomly assigned, in equal numbers, adults 18 to 45 years of age in whom T. trichiura or hookworm eggs had been detected in stool samples to receive emodepside, at a single oral dose of 5, 10, 15, 20, 25, or 30 mg; albendazole, at a single oral dose of 400 mg; or placebo. The primary outcome was the percentage of participants who were cured of T. trichiura or hookworm infection (the cure rate) with emodepside 14 to 21 days after treatment, determined with the use of the Kato–Katz thick-smear technique. Safety was assessed 3, 24, and 48 hours after the receipt of treatment or placebo.ResultsA total of 266 persons were enrolled in the T. trichiura trial and 176 in the hookworm trial. The predicted cure rate against T. trichiura in the 5-mg emodepside group (85% [95% confidence interval {CI}, 69 to 93]; 25 of 30 participants) was higher than the predicted cure rate in the placebo group (10% [95% CI, 3 to 26]; 3 of 31 participants) and the observed cure rate in the albendazole group (17% [95% CI, 6 to 35]; 5 of 30 participants). A dose-dependent relationship was shown in participants with hookworm: the observed cure rate was 32% (95% CI, 13 to 57; 6 of 19 participants) in the 5-mg emodepside group and 95% (95% CI, 74 to 99.9; 18 of 19 participants) in the 30-mg emodepside group; the observed cure rates were 14% (95% CI, 3 to 36; 3 of 21 participants) in the placebo group and 70% (95% CI, 46 to 88; 14 of 20 participants) in the albendazole group. In the emodepside groups, headache, blurred vision, and dizziness were the most commonly reported adverse events 3 and 24 hours after treatment; the incidence of events generally increased in a dose-dependent fashion. Most adverse events were mild in severity and were self-limited; there were few moderate and no serious adverse events.ConclusionsEmodepside showed activity against T. trichiura and hookworm infections. (Funded by the European Research Council; ClinicalTrials.gov number, NCT05017194.)