Types Of Honey Research Articles

The Antioxidant Activity of Monofloral Honey and Its Awareness Among Urban Consumers in Slovakia

The main objective of this paper was to determine antioxidant activity in various types of monofloral honey produced in Slovakia and to identify consumer behaviour and awareness of biological activity of honey among urban consumers. Research is based on primary data obtained by conducting analytical research (DPPH and TPC) involving 37 samples of monofloral honey and consumer research (n =1940 Slovak honey consumers living in urban areas). Results showed that the highest antioxidant activity was detected in buckwheat honey and honeydew honey, while the lowest was indicated in acacia honey. Consumer research showed that 57.5% of respondents are aware of antioxidant activity in honey and 12,9% indicated that they are not aware of any biological activity. Urban consumers tend to consume honey few times per month or week. Higher annual consumption is typical for consumers older than 42 years. The most preferred honey is acacia honey followed by honeydew and linden honey. The majority of respondents seeks on the honey label information about honey type, producer and origin while the information about nutrition or additional information (website, social media etc.) are not so important.

Phenolic Profiles, Antioxidant, Antibacterial Activities and Nutritional Value of Vietnamese Honey from Different Botanical and Geographical Sources

Honey is a natural product made by honeybees, its composition depends on factors such as climate, soil and plant source. In this study, the nutritional parameters, phenolic composition, antioxidant activity and antibacterial ability of 30 different types of honey of different botanical and geographical origins in Vietnam were investigated. The study focused on the characterization and evaluation of the influence of plant origin and geographical location on physical–chemical properties and biological activities (antioxidant and antibacterial). The obtained results show that all honey samples meet quality standards according to international standards and Vietnamese standards, except for some exceptions recorded in moisture, 5-hydroxymethylfurfural (HMF) value and ash. These samples were explored for the detection of 13 polyphenols by using high-performance liquid chromatography (HPLC). The classification of honey samples collected from different regions and botanical sources was performed by principal component analysis (PCA), and it was observed that certain phenolic compounds contributed to the identification of honey samples. In addition, the correlation between physicochemical properties, chemical composition and biological activity of most honeys was also first clarified in this study. Overall, our data provide an overview data set and essential results in creating a database on the world honey trait map.

Impact of genus (Geotrigona, Melipona, Scaptotrigona) on the targeted 1H-NMR organic profile, and authenticity test by interphase emulsion of honey processed in cerumen pots by stingless bees in Ecuador

The biodiversity of Ecuadorian stingless bees is almost 200 species. Traditional pot-honey harvest in Ecuador is mostly done from nests of the three genera selected here Geotrigona Moure, 1943, Melipona Illiger, 1806, and Scaptotrigona Moure, 1942. The 20 pot-honey samples collected from cerumen pots and three ethnic honeys “abeja de tierra”, “bermejo”, and “cushillomishki” were analyzed for qualitative and quantitative targeted 1H-NMR honey profiling, and for the Honey Authenticity Test by Interphase Emulsion (HATIE). Extensive data of targeted organic compounds (41 parameters) were identified, quantified, and described. The three honey types were compared by ANOVA. Amino acids, ethanol, hydroxymethylfurfural, aliphatic organic acids, sugars, and markers of botanical origin. The number of phases observed with the HATIE were one in Scaptotrigona and three in Geotrigona and Melipona honeys. Acetic acid (19.60 ± 1.45 g/kg) and lactic acid (24.30 ± 1.65 g/kg) were particularly high in Geotrigona honey (in contrast to 1.3 g/kg acetic acid and 1.6 g/kg lactic acid in Melipona and Scaptotrigona), and with the lowest fructose + glucose (18.39 ± 1.68) g/100g honey compared to Melipona (52.87 ± 1.75) and Scaptotrigona (52.17 ± 0.60). Three local honeys were tested using PCA (Principal Component Analysis), two were assigned with a correct declared bee origin, but “bermejo” was not a Melipona and grouped with the Scaptotrigona cluster. However after HCA (Hierarchical Cluster Analysis) the three honeys were positioned in the Melipona-Scaptotrigona cluster. This research supports targeted 1H-NMR-based profiling of pot-honey metabolomics approach for multi-parameter visualization of organic compounds, as well as descriptive and pertained multivariate statistics (HCA and PCA) to discriminate the stingless bee genus in a set of Geotrigona, Melipona and Scaptotrigona honey types. The NMR characterization of Ecuadorian honey produced by stingless bees emphasizes the need for regulatory norms. A final note on stingless bee markers in pot-honey metabolites which should be screened for those that may extract phylogenetic signals from nutritional traits of honey. Scaptotrigona vitorum honey revealed biosurfactant activity in the HATIE, originating a fingerprint Honey Biosurfactant Test (HBT) for the genus in this set of pot-honeys.

Identification of the Main Phenolic Markers in Turkish Pine Honeys and Their Biological Functions.

The purpose of this research was to identify the biological activity of Turkish pine honeys and the most abundant phenolic compound(s) to guide future studies to be done about this type of honey. Moreover, this will provide the chance to introduce Turkish pine honeys by elucidating a distinct parameter compared to other forms of honey reported in the literature. To this end, microscopic examination based on the number of honeydew elements (NHE) and the number of total pollen (NTP) grains was performed on 17 samples of pine honeys from the Aegean region of Turkey. Then 18 commonly-occurring phenolic standards were used in high-performance liquid chromatography (HPLC) to examine the samples. Each sample was tested for individual phenolic compounds, as well as total phenolic content (TPC), total flavonoid content (TFC), and ferric reducing antioxidant power (FRAP). As shown by the numerical data, the values for NHE and NTP were 6600-144000 and 3000-75000, respectively. In addition, the general means of TPC, TFC, and FRAP values for the samples were 53.000 mg GAE/100 g fresh sample, 1.944 mg QE/100 g fresh sample, and 3.455 μmol FeSO4 /g fresh sample, respectively. Protocatechuic acid predominated with a range of 197.841-780.227 μg phenolic /100 g sample in all samples. According to the general mean for values detected in the samples, p-hydroxybenzoic acid and benzoic acid were the second and third markers for Turkish pine honey samples. It is believed that these results will help efforts to set standards for pine honey on a national and global scale.

An Investigation into Corsican Honey: Chemical Composition and Biological Activities

The present work studied five corsican honey varieties (spring maquis, autumn maquis, spring honey, summer maquis, and blended honey) and the correlation of their chemical composition, antioxidant activity, and ability to inhibit enzymes (acetylcholinesterase [AChE] and tyrosinase), which are associated with several diseases, such as Alzheimer’s disease. Analysis using headspace-solid phase microextraction (HS-SPME)/Gas chromatography (GC)/GC-Mass spectrometry (MS) indicated that the samples presented different chemical profiles. The main components of spring maquis honey are 4-propylanisol (14.7%) and p-anisaldehyde (11.7%). For autumn maquis honey, they are isophorone (34.8%) and 3,4,5-trimethylphenol (27.1%), and for spring honey, they are phenylacetaldehyde (15.8%) and (2R, 2’S, 5’S)-lilac aldehyde (10.1%). For blended honey, they are 3-furaldehyde (13.9%) and 4-propylanisol (10.8%), and for summer maquis honey, they are phenylacetaldehyde (33.6%) and benzaldehyde (9.1%). All the types of honey studied showed antioxidant activity in the three tests studied. The most potent honey in the DPPH test (IC50 = 73.69 µg/mL), the reducing iron test (IC50 = 45.12 µg/mL), and the β-carotene test (IC50 = 53.80 µg/mL) was autumn scrub honey. All the types of honey studied have inhibitory capacities on acetylcholinesterase and tyrosinase. Our study found that corsican honey is a rich source of organic compounds that could positively impact health.

Characteristics of Forest and Manuka Honey As Well As Their Application As Herbal Honey Drinks With The Addition of Qusthul Hindi and Turmeric

Manuka and forest honeys are types of honeys that are widely consumed because for their antioxidant properties. In this study, the properties of forets and manuka honey and the use of honey as a herbal drink with the addition of Qusthul hindi and turmeric were analyzed. The addition of Qusthul hindi (0,125 g and 0,375 g) and turmeric (0,125 g and 0,375 g) is given in two formulations. This study was conducted in two phase, namely analyzing the characteristics of manuka and forest honey and preparing herbal drinks to then analyzing their properties. The results showed that the properties of forest honey do not meet the standards for the variables of pH, HMF value, and diastase enzyme activity, while Manuka honey meets the standards only for variables of moisture, HMF value, and diastase enzyme activity. The addition of Qusthul hindi and turmeric to herbal drinks results in an increase in pH, DPPH inhibitoty activity, and antioxidant capacity, as well as a decrease of water content, but is not sensory evaluated because the addition of spices causes changes in taste and appearance.

Comparable Benefits of Stingless Bee Honey and Caffeic Acid in Mitigating the Negative Effects of Metabolic Syndrome on the Brain.

There is mounting evidence that metabolic syndrome (MetS) contributes to the development of neurodegenerative disorders such as Alzheimer's disease. Honey, which has been used for generations, is high in antioxidants and has been demonstrated to benefit the brain and mental health by reducing oxidative stress and boosting cognitive outcomes. Honey from the stingless bees of Heterotrigona itama has been found to have higher phenolic content compared to other types of honeys. The aim of this study is to investigate the effects of stingless bee honey (SBH) supplementation and to compare it with a pure form of antioxidant, caffeic acid (CA), on MetS parameters and inflammatory markers in the brains of MetS-induced rats. A total of 32 male Wistar rats were divided equally into groups of control, high-carbohydrate high-fructose (HCHF) diet (MetS), HCHF + SBH supplemented (1 g/kg) (SBH), and HCHF + CA supplemented (10 mg/kg) (CA) groups. The total duration for SBH and CA supplementation was eight weeks. The HCHF diet was found to promote hypertension, hyperglycemia, and hypertriglyceridemia, and to increase brain TNF-α levels. Supplementation with SBH and CA significantly reversed (p < 0.05) the hyperglycemic and hypertensive effects of the HCHF diet. Although both supplemented groups showed no significant changes to serum HDL or TG, SBH significantly reduced (p < 0.05) brain TNF-α levels and increased (p < 0.05) brain BDNF levels. Immunohistochemistry investigations of neurogenesis (EdU) and apoptosis (TUNEL) on the cornu Ammonis 1 (CA1) and dentate gyrus (DG) areas of the hippocampus showed no changes with SBH and CA supplementation compared to the control. These findings suggest that SBH and CA have the potential to mitigate HCHF-induced MetS effects and possess neuroprotective abilities.

Isolation of Gram Positive Bacteria In Sumbawa Wild Honey

Sumbawa is one of the areas known as honey-producing areas in Indonesia, one of which is wild honey. Wild honey is a type of honey whose habitat is in the wild. How to find honey in Sumbawa is still fairly traditional. The process of packaging wild honey is also still using the traditional way from taking to packaging which is still traditional, which allows bacterial contamination of honey. The aim of the study was to isolate gram positive in Sumbawa wild honey. This study used 10 honey samples from Sumbawa which were used for isolation of gram-positive bacteria in Sumbawa wild honey and then accommodated in a sample tube. Based on the results of the study, it can be concluded that from 10 samples of Sumbawa wild honey taken from different villages there were gram-positive bacteria.

Establishment and application of quantitative method for 22 organic acids in honey based on SPE-GC–MS

Honey, a natural healthy liquid bee product, is rich in amino acids, vitamins, and other essential nutrients. Different origin honeys also varied in organic acids. The objective of this study is to establish an efficient solid-phase extraction-gas chromatography–mass spectrometry (SPE-GC–MS) method to eliminate interference of sugar and other impurities for accurately estimating 22 organic acids in honey by optimizing extraction, purification, derivatization, and gas chromatography–mass spectrometry (GC–MS) analytical conditions. After being extracted, purified and derivatized, organic acids in honey were qualified and quantified by GC–MS. This method was evaluated experimentally, and the results showed that, within a certain concentration range, the standard curve linear relationship was satisfactory (R2 > 0.9942), and the target organic acid recovery rate was 86.74% ~ 118.68%. Besides, precision (relative standard deviation, RSD = 2.98% ~ 13.42%), detection limit (LOD = 0.002 ~ 0.2 mg kg−1), and quantification limit (LOQ = 0.008 ~ 0.5 mg kg−1) met the target requirements. Also, based on this analytical method, the organic acids in five types of honey (acacia, jujube, vitex, canola and linden honey) were estimated. Notably, they all contained 22 different kinds of organic acids, and significant differences (p < 0.05) in the organic acid content and composition among different honey varieties were observed. PCA analysis showed that the five honeys could be differentiated based on the content of 22 organic acids.

Semi-Quantitative Discrimination of Honey Adulterated with Cane Sugar Solution by an ETongue.

This study successfully applied a potentiometric E-tongue with 20 cross-selectivity lipidic polymeric membranes in the discrimination of three semi-quantitative groups, that represented the following intervals of honey adulteration percentage with cane sugar: 0 %; [0, 10]%; [10, 20]% of adulteration. We analysed five different types of Portuguese honey; five brands of cane sugar were added to the adulterated samples; a comparative analysis was then performed. Linear discriminant analysis coupled with a tabu search algorithm for feature selection was applied to the ETongue's analytical data to select the best model. A discriminant model with 12 sensors was obtained. This model classified correctly all samples in both in internal (train data, 15 samples) and external validation (test data,10 samples). Also, multiple linear regression with tabu search was applied to verify if ETongue's data would allow quantifying the honey's adulteration level. The results showed that it was possible to obtain a quantitative model but with unsatisfactory predictive performance in the test data group (external validation), giving, in general, values below the expected concentrations. E-tongue is a real-time green, flexible and low-cost analytical tool that requires minimum sample preparation and no special technical skills, being a promising tool for everyday application.

Determination of biomarkers of buckwheat honey By solid-phase microextraction in order to establish authenticity

Honey production in our country has increased compared to the last decade. East Kazakhstan, Almaty and South Kazakhstan regions are the main producers of honey. The floral diversity of our country makes it possible to obtain many types of monofloral honey and several combinations of polyfloral honey. Floral origin is one of the most important factors influencing the honey market. Monofloral honey is more popular among consumers due to its taste, special characteristics and is more profitable for producers due to its high price. Darker honey (buckwheat honey) usually contains high amounts of antioxidants, which increase its health potential. Food control is a major factor for consumers who want to know that their purchases are of high quality. To ensure that the actual composition of the products indicated on the label, analysis and development of effective methods of analysis is necessary. Therefore, the aim of this study is to identify biomarkers of buckwheat honey by vacuum solid phase microextraction (vac-SPME) in combination with gas chromatography-mass spectrometry (GC-MS).

Characterization of Rare Himalayan Balsam (Impatiens glandulifera Royle) Honey from Croatia.

Himalayan balsam (Impatiens glandulifera Royle) is an invasive garden ornamental plant species originating from Asia, which produces significant amounts of nectar. In Croatia, it is widely distributed along the banks of the Mura River. Although this plant species is widespread in Europe, there are still no available scientific data about this unifloral honey type. The results showed that Himalayan balsam honey is characterized by the high presence of pollen grains in the pollen spectrum (59–85%), natural higher diastase activity (39.1 ± 7.98 DN), negative specific rotation (−21.2° ± 6.89) and an extra light amber color (48.5 ± 12.69 mm Pfund). The carbohydrate profile is characterized by monosaccharides fructose (39.34 ± 0.65 g/100 g) and glucose (31.91 ± 1.42 g/100 g) with a ratio >1.23, while the most commonly represented disaccharide was maltose (3.04 ± 0.79 g/100 g). The average total phenolic content was 130.97 ± 11.17 mg gallic acid/kg honey, and the average antioxidant capacity value was 225.38 ± 29.58 µM Fe(II). The major mineral element was K, with an average of 533.92 ± 139.70 mg/kg. The sensory profile was characteristic with a light orange color and medium-intensity odor and aroma. The crystallization rate was moderate and characterized by the appearance of opalescence and gelatinous forms of crystals. The results of this study provide the first insight into the melissopalynological, physico-chemical and sensory profile of Himalayan balsam honey.

Sugars and °Brix in honey from Apis mellifera, Melipona beecheii, and commercial honey from a local market in Mexico

Fructose and glucose are the main sugars in honey, and their concentration is expected to correspond to the specifications of official standards. The study compared the composition of sugars and ºBrix in honey from Apis mellifera and Melipona beecheii bees, and a product marketed as bee honey in a local market. The sugar content was determined by highperformance liquid chromatography (HPLC) with a refractive index detector, and °Brix was determined using a refractometer. None of the honey analyzed had detectable levels of sucrose. The average concentration of fructose and glucose in honey was 36.4 and 28.9 g 100 g-1 for Apis mellifera, and 38.5 and 28.2 g 100 g-1 for honey from Melipona beecheii. For honey from the local market, the respective concentrations of these sugars were 7.5 and 17.0 g 100 g-1. The fructose:glucose ratio (F:G) was higher than one in Apis mellifera and Melipona beecheii honey, and 0.4 for honey from the local market. The three types of honey compared had similar values for °Brix. It is concluded that the honey under study by Apis mellifera and Melipona beecheii have similar qualities within international standards. In contrast, the product marketed as bee honey in the local market did not meet official regulations specifications and could not be considered authentic.

Effects of Apis Mellifera, Apis Cerana, and Trigona SP on 20 M Sprint Performance

This study aimed to determine the effect of three types of honey from three different honey bee species on increasing stamina and anti-fatigue effect by examining their effects on the biochemical process in the anaerobic pathway. Tests were carried out on humans who had met the specified criteria. The method used in this study was an experimental method involving 30 healthy students. The inclusion requirements consisted of males aged 18-20 years, weight 50-70 kilograms, height 160-170 cm, normal momentary blood sugar levels 140 mg/dL, VO2max 35-40 ml/kg/min, normal blood pressure, and not having severe diseases such as diabetes, asthma, kidney failure, hepatitis, as well as other serious diseases that hinder physical activity. Volunteers had also filled out informed consent stating their willingness to participate in the research to completion. The results of this study showed that there was a change between pre and post in each groups, indicating that each honey content had a significant impact on improving the participant performance. In addition, this study found changes in the three types of honey (Apis Mellifera, Apis Cerana, Trigona SP) in the participant running performance improvement. However, there was no significant difference between the three types of honey.

Determination of the parameters of freeze-drying honey

The article presents the results of studies of freeze-drying of various types of honey. The purpose of the research was to determine the optimal parameters for the freeze-drying of honey. The physical and chemical parameters of three types of honey produced in the East Kazakhstan region were previously studied. The results showed that the studied prototypes meet the regulatory requirements for the quality of natural honey. It has been established that in terms of moisture content, sunflower honey differs from mountain honey and sweet clover (15 %, 17.3 % and 17.2 %, respectively), and also contains a high amount of reducing sugars (87.9 %), however, the mass fraction of sucrose is 2.5 %. It is noted that the highest content of sucrose in mountain honey is 4.5 %. The diastase number is an indicator of the quality and naturalness of honey and, according to the requirements of GOST, should be at least 8 Gote units, the results show that the studied samples of honey contain from 13.1 to 15.1 units, which confirms the influence of the geographical origin of honey. According to the value of water activity, all types of honey are products with low humidity and long-term storage. The optimal technological modes of freeze-drying of honey have been established, which range from minus 30 °C to minus 40 °C degrees.

Honey and Other Beekeeping Products Intake among the Romanian Population and Their Therapeutic Use

Honey and other bee products have been used for food and therapeutic purposes since ancient times. There are many varieties of honey with time-proven therapeutic properties, used in both traditional and modern medicine, along with various beekeeping products. In this study, conducted based on the dissemination of a questionnaire with 43 questions, an evaluation of the consumption of different types of honey for both food and therapeutic purposes was carried out. Hence, the frequency of the consumption of honey for food purposes was evaluated, as well as the pharmaceutical forms of honey and bee products utilized for therapeutic purposes, the population’s trust in their therapeutic potential, and their trust in the quality of bee products among the Romanian population. After processing the data, 917 responses were recorded, and it was found that the preferred types of honey were black locust (83.5%), multi-floral (81.9%), and linden (74.9%), and among the other bee products, the most consumed were propolis (44.2%) and bee pollen (29.2%). Regarding the use of honey as a sweetener, the majority of the respondents considered honey to be the healthiest option (81.7%), and regarding the use of honey for therapeutic purposes, most of the respondents stated that they used honey to strengthen the immune system (65.4%), as well as for skin treatment, laxative action, or energizing. The centralization and processing of the collected responses indicated a considerable level of readiness related to the increase in honey consumption in particular, but there is a need for the dissemination of effective information related to the nutritional and therapeutic value of beekeeping products to the population.

Different Concentrations of Honey's Antimicrobial Activity Against ‎Staphylococcus Aureus by HPLC Determination

Honey has been used in ancient times as a treatment. It is used for healing wounds and also can be used as antiseptic to kill bacteria and other microorganisms. The aim of this study was to examine the activity of honey as a bactericidal and bacteriostatic and to measure the most active compounds of honey with highly bacterial inhibition zone using HPLC technique. In this experiment and the honey was used to check whether the honey can be used as an antimicrobial agent or not. First by well diffusion method is done by adding different concentrations of honey (25, 75, 100, 125, 150, 200, microliter). Th results showed a little inhibition zone for the three types of honey but in different size for each type. The industrial honey showed the largest effect. Different concentrations were used (10 %, 30 % 50%). The antibiotic sensitivity was applied, the result showed only two resistant antibiotics (Azithromycin and Erythromycin). The concentration of Catalase, Amylase, Invertase, Glucose oxidase in honey tested by HPLC and showed the concentration of each substance cons of catalase = (32877 ÷ 170253 ) x 20 = 3.862 u/ml; cons of amylase = (136985 ÷ 180849) x 20 = 15.149 u/ml; cons of Invertase = (58466 ÷ 193624) x 20 = 6.039 u/ml; cons of glucose peroxide = (105204 ÷ 163245) x 20 =12.889 u/ml. Moreover, the presence of gluconic acid ‘Organic acid’ gives the honey its acidic characteristic which is about 3.2 pH.

¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey.

The purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (Morcott tangerine L. and Jaffa orange L.), marjoram honey (HM1) (Origanum majorana L.), and clover honey (HT1) (Trifolium alexandrinum L.), compared to their secondary metabolites (HC2, HM2, HT2, respectively). By using a ¹H-NMR-based metabolomic technique, PCA, and PLS-DA multivariate analysis, we found that HC2, HM2, HC1, and HM1 were clustered together. However, HT1 and HT2 were quite far from these and each other. This indicated that HC1, HM1, HC2, and HM2 have similar chemical compositions, while HT1 and HT2 were unique in their chemical profiles. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH, ABTS, ORAC, 5-LOX, and metal chelating activity in all honey extract samples and their secondary metabolites. Our results revealed that HC2 and HM2 possessed more antioxidant activities than HT2 in vitro. HC2 demonstrated the highest antioxidant effect in all assays, followed by HM2 (DPPH assay: IC50 2.91, 10.7 μg/mL; ABTS assay: 431.2, 210.24 at 50 ug/mL Trolox equivalent; ORAC assay: 259.5, 234.8 at 50 ug/mL Trolox equivalent; 5-LOX screening assay/IC50: 2.293, 6.136 ug/mL; and metal chelating activity at 50 ug/mL: 73.34526%, 63.75881% inhibition). We suggest that the presence of some secondary metabolites in HC and HM, such as hesperetin, linalool, and caffeic acid, increased the antioxidant activity in citrus and marjoram compared to clover honey.

Phenolic compounds and color of labeled resin spurge honey and their correlations with pollen content

Our aim in this study was to outline phenolic and color characteristic that characterize the labeled unifloral resin spurge (Euphorbia resinifera) honey. With respect to phenolic composition, 17 phenolic compounds have been analyzed in the 29 honey samples. The proposed markers (syringic acid, ethyl gallate, m-coumaric acid and naringenin) might help to the enhancement of this honey type and thus, guarantee its commercial value. The color characterization by diffuse reflectance spectrophotometry revealed typical values of light amber honey (lightness ranged from 36 to 70 units, and chroma from 18 to 30 units). On the other hand, many correlations between the color attributes and phenolic acids, total phenolic compounds, caffeic acid, p- and m-coumaric acids and hesperidin have been demonstrated, also, correlation between phenols, color parameters and percentage of pollen of E. resinifera has been found. This study is one of the rare researches which have correlated the CIELAB color parameters with the individual phenolic acids and flavonoid compounds within the same unifloral honey.

Botanical origin identification and adulteration quantification of honey based on Raman spectroscopy combined with convolutional neural network

A non-destructive and reliable spectroscopic analysis method was proposed for detecting honey fraud based on Raman spectroscopy and convolutional neural network (CNN). Acacia, litchi and linden honey were adulterated with high fructose corn syrup (HFCS), rice syrup (RS), maltose syrup (MS) and commercial blended syrup (BS) at different concentrations, respectively. Spectra were collected from 60 authentic honeys and 360 adulterated honeys. Dimensionality reduction algorithms provided an overview and visualization of the spectral dataset. The strategy of this study was that the botanical origins and adulteration concentrations of unknown samples were obtained through a combination of qualitative and quantitative models, regardless of the types of honey and contaminant. The CNN classification model and chemometric algorithms achieved more than 99.76% accuracy for honey matrix identification, while the CNN quantitative models kept the coefficients of determination (R2p) and root mean square errors of prediction (RMSEP) above 0.95 and below 4.25, respectively. CNN achieved significantly better performance than chemometric algorithms and met the routine detection requirements of honey. The proposed method provides a promising alternative to combat honey fraud.