Managed Honey Bee Research Articles

Glucosinolate Bioactivation by Apis mellifera Workers and Its Impact on Nosema ceranae Infection at the Colony Level.

The microsporidian fungus Nosema ceranae represents one of the primary bee infection threats worldwide and the antibiotic fumagillin is the only registered product for nosemosis disease control, while few alternatives are, at present, available. Natural bioactive compounds deriving from the glucosinolate–myrosinase system (GSL–MYR) in Brassicaceae plants, mainly isothiocyanates (ITCs), are known for their antimicrobial activity against numerous pathogens and for their health-protective effects in humans. This work explored the use of Brassica nigra and Eruca sativa defatted seed meal (DSM) GSL-containing diets against natural Nosema infection in Apis mellifera colonies. DSM patties from each plant species were obtained by adding DSMs to sugar candy at the concentration of 4% (w/w). The feeding was administered in May to mildly N. ceranae-infected honey bee colonies for four weeks at the dose of 250 g/week. In the treated groups, no significant effects on colony development and bee mortality were observed compared to the negative controls. The N. ceranae abundance showed a slight but significant decrease. Furthermore, the GSL metabolism in bees was investigated, and MYR hydrolytic activity was qualitatively searched in isolated bee midgut and hindgut. Interestingly, MYR activity was detected both in the bees fed DSMs and in the control group where the bees did not receive DSMs. In parallel, ITCs were found in gut tissues from the bees treated with DSMs, corroborating the presence of a MYR-like enzyme capable of hydrolyzing ingested GSLs. On the other hand, GSLs and other GSL hydrolysis products other than ITCs, such as nitriles, were found in honey produced by the treated bees, potentially increasing the health value of the final product for human consumption. The results are indicative of a specific effect on the N. ceranae infection in managed honey bee colonies depending on the GSL activation within the target organ.

Wild Bees and their Conservation

About sustainable agricultural environment and world food security, major crops mainly dependent upon managed honey bees and wild bee pollinators which are of great significance. Bee pollinated crops being a great part of the bio-diverse system, 4,000 native bees, and offer over US$1.5 billion each year in North America. In US, the value of wild bees in food production was determined to be over $1.5 billion annually. However, the worth of wild bee pollination in insect cross pollinated crops may be much more. These great wild players are now in fast declining phase or possibly extinct due to human-disturbed habitats. More investigations are required in various topics of wild bee fauna, such as basic studies in population biology, abundance, bee protection measures, suitable habitat, nesting sites especially their immediate conservation strategies. After realizing the importance of wild bees in pollination, in the present review, we highlight the various measures and actions to conserve the wild bees so that they can serve the growers as co-players to managed honey bees in boosting the agricultural food production worldwide.

IPM reduces insecticide applications by 95% while maintaining or enhancing crop yields through wild pollinator conservation

Pest management practices in modern industrial agriculture have increasingly relied on insurance-based insecticides such as seed treatments that are poorly correlated with pest density or crop damage. This approach, combined with high invertebrate toxicity for newer products like neonicotinoids, makes it challenging to conserve beneficial insects and the services that they provide. We used a 4-y experiment using commercial-scale fields replicated across multiple sites in the midwestern United States to evaluate the consequences of adopting integrated pest management (IPM) using pest thresholds compared with standard conventional management (CM). To do so, we employed a systems approach that integrated coproduction of a regionally dominant row crop (corn) with a pollinator-dependent specialty crop (watermelon). Pest populations, pollination rates, crop yields, and system profitability were measured. Despite higher pest densities and/or damage in both crops, IPM-managed pests rarely reached economic thresholds, resulting in 95% lower insecticide use (97 versus 4 treatments in CM and IPM, respectively, across all sites, crops, and years). In IPM corn, the absence of a neonicotinoid seed treatment had no impact on yields, whereas IPM watermelon experienced a 129% increase in flower visitation rate by pollinators, resulting in 26% higher yields. The pollinator-enhancement effect under IPM management was mediated entirely by wild bees; foraging by managed honey bees was unaffected by treatments and, overall, did not correlate with crop yield. This proof-of-concept experiment mimicking on-farm practices illustrates that cropping systems in major agricultural commodities can be redesigned via IPM to exploit ecosystem services without compromising, and in some cases increasing, yields.

Dominance of honey bees is negatively associated with wild bee diversity in commercial apple orchards regardless of management practices

Commercial apple production relies on managed honey bees (Apis mellifera) for pollination, and on intensive management for pest control. Previous studies have highlighted the potentially detrimental effects of intensive crop management on wild bee diversity in agroecosystems, potentially jeopardizing the pollination services they provide. However, the extent to which honey bee dominance and crop management interact under field-realistic conditions and drive the structure of wild bee assemblages has not been investigated so far. In this study, we measured species richness, as well as the functional and phylogenetic diversity of wild bee assemblages in 36 paired organic and non-organic apple orchards during their flowering season and along a geographic gradient across western Europe. Our results show a strong significant and negative association between honey bee dominance and all wild bee diversity metrics, regardless of local management. Semi-natural habitats had a significant and positive effect on functional diversity, while urbanization and crop cover around the orchards showed no effect on all measured diversity metrics. A greater number of species exhibited less common, or frequent, combinations of functional traits at sites with high honey bee dominance, especially larger bee species with longer tongues. Collectively, we show that wild bee diversity decreases with increasing honey bee dominance, and that this negative association is not buffered by alternative (i.e., organic) management practices in commercial apple orchards. Although organic farming can bring about biodiversity benefits, our study demonstrates that, in the context of commercial apple production, other measures are needed to enhance and harness biodiversity for sustainable and profitable crop production. In particular, a lowered reliance on honey bees and a redesign of orchards through configurational crop heterogeneity and/or the restoration of in-field semi‐natural elements are required beyond agricultural input substitution.

Detection of Microsporidia in Pollinator Communities of a Mediterranean Biodiversity Hotspot for Wild Bees.

Insect pollination is crucial for the maintenance of natural and managed ecosystems but the functioning of this ecosystem service is threatened by a worldwide decline of pollinators. Key factors in this situation include the spread and interspecific transmission of pathogens worldwide through the movement of managed pollinators. Research on this field has been mainly conducted in some particular species, while studies assessing the interspecific transmission of pathogens at a community level are scarce. However, this information is pivotal to design strategies to protect pollinators. Herein, we analysed the prevalence of two common microsporidia pathogens of managed honey bees (Nosema ceranae and N. apis) in bee communities of semiarid Mediterranean areas from the Southeast of the Iberian Peninsula. Our results confirm the ability of N. ceranae to disperse across wild bee communities in semiarid Mediterranean ecosystems since it was detected in 36 Apoidea species (39% of the sampling; for the first time in nine genera). The prevalence of the pathogen did not show any phylogenetic signal which suggests a superfamily host range of the pathogen or that wild bees may be acting only as vectors of N. ceranae. In addition, N. apis was detected in an Eucera species, which is the second time it has been detected by molecular techniques in a host other than the honey bee. Our study represents the primary assessment of the prevalence of microsporidia at community level in Mediterranean areas and provides outstanding results on the ability of Nosema pathogens to spread across the landscape.

Honey bee viruses are highly prevalent but at low intensities in wild pollinators of cucurbit agroecosystems

Managed and wild bee populations are in decline around the globe due to several biotic and abiotic stressors. Pathogenic viruses associated with the Western honey bee (Apis mellifera) have been identified as key contributors to losses of managed honey bee colonies, and are known to be transmitted to wild bee populations through shared floral resources. However, little is known about the prevalence and intensity of these viruses in wild bee populations, or how bee visitation to flowers impacts viral transmission in agroecosystems. This study surveyed honey bee, bumble bee (Bombus impatiens) and wild squash bee (Eucera (Peponapis) pruinosa) populations in Cucurbita agroecosystems across Pennsylvania (USA) for the prevalence and intensity of five honey bee viruses: acute bee paralysis virus (ABPV), deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), and slow bee paralysis virus (SBPV). We investigated the potential role of bee visitation rate to flowers on DWV intensity among species in the pollinator community, with the expectation that increased bee visitation to flowers would increase the opportunity for transmission events between host species. We found that honey bee viruses are highly prevalent but in lower titers in wild E. pruinosa and B. impatiens than in A. mellifera populations throughout Pennsylvania (USA). DWV was detected in 88% of B. impatiens, 48% of E. pruinosa, and 95% of A. mellifera. IAPV was detected in 5% of B. impatiens and 4% of E. pruinosa, compared to 9% in A. mellifera. KBV was detected in 1% of B. impatiens and 5% of E. pruinosa, compared to 32% in A. mellifera. Our results indicate that DWV titers are not correlated with bee visitation in Cucurbita fields. The potential fitness impacts of these low viral titers detected in E. pruinosa remain to be investigated.

Honey bee (Apis mellifera) colonies benefit from grassland/ pasture while bumble bee (Bombus impatiens) colonies in the same landscapes benefit from non-corn/soybean cropland.

Agriculturally important commercially managed pollinators including honey bees (Apis mellifera L., 1758) and bumble bees (Bombus impatiens Cresson, 1863) rely on the surrounding landscape to fulfill their dietary needs. A previous study in Europe demonstrated that managed honey bee foragers and unmanaged native bumble bee foragers are associated with different land uses. However, it is unclear how response to land use compares between managed honey bees and a managed native bumble bee species in the United States, where honey bees are an imported species. Furthermore, to our knowledge, no such direct comparisons of bee responses to land use have been made at the colony level. To better understand how two different social bees respond to variation in land use, we monitored the weights of A. mellifera and B. impatiens colonies placed in 12 apiaries across a range of land use in Michigan, United States in 2017. Bombus impatiens colonies gained more weight and produced more drones when surrounded by diverse agricultural land (i.e., non-corn/soybean cropland such as tree fruits and grapes), while honey bee colonies gained more weight when surrounded by more grassland/pasture land. These findings add to our understanding of how different bee species respond to agricultural landscapes, highlighting the need for further species-specific land use studies to inform tailored land management.

Standardization of managed honey bee (Apis mellifera) hives for pollination of Sunflower (Helianthus annuus) crop

To determine the effect of honey bee (Apis mellifera L.) pollination on sunflower yield using the most appropriate number of beehives per unit area of crops is very important. By comparing the number of hives and yield components, we can satisfy optimal pollination needs and improve economic yields. For this purpose, a series of experiments were conducted at thatha Khalil farmer field Taxila Rawalpindi, under the supervision of Beekeeping and Hill Fruit Pests Research, Station Rawalpindi, Pakistan, with four treatments for comparison: 1 hive acre−1, 2 hives acre−1, 3 hives acre−1, and 0 hives acre−1 as a control. The hives were kept inside the experimental area and pollination density, pollinator’s diversity, agronomic yield, and economic yield were determined. Two acres of sunflower field were sown and four bee colonies were introduced at 5% to 10% flowering. The maximum visitation frequency of A. mellifera was recorded at the weekly interval from 24 to 09-2019 to 24–10-2019, according to which maximum visitation was observed (44.8, 38.4, and 16.5 plant−1 5 min−1) at 10:00, 12:00, and 14:00 hrs, respectively. Data regarding foraging pollinators revealed that A. mellifera was the most dominant pollinator of the sunflower crop with the highest abundance 17.11% followed by A. cerana (3.46%) and the population of A. dorsata was minimum (2.12%). Furthermore, agronomic parameters (number of seeds plant−1, 100 seed weight (g), and economic yield were significantly higher in those fields which were pollinated by more beehives in comparison to fewer beehives.

Commoning the bloom? Rethinking bee forage management in industrial agriculture

Managed and wild bee populations are declining around the world, in part due to lost access to bee forage (i.e., nectar and pollen). As bee forage diminishes, the remaining acres become sites of contestation between beekeepers, land managers, ecologists, and regulatory agencies. This article applies a commons framing to contextualize these conflicts and attempts to resolve them. Drawing from the concepts of commons and commoning, I argue that nectar and pollen are common-pool resources for pollinators, beekeepers, and land managers, currently managed through varied access arrangements such as informal usufruct rights and pseudo-commoning practices. Like commoning, pseudo-commoning aims to collectively manage a resource through a set of protocols that involve equitable resource sharing and communication. However, because pseudo-commons are implemented from the top down, for example, from institutional actors driven in part by economic interests, they often do not result in widespread adoption on the ground. Through a case in California almond orchards, I make two additional arguments. First, because beekeepers are largely migratory and do not own the land they need for production, their subordinate position to landowners can challenge equitable bee forage management. Second, while floral pseudo-commons may aim to counter the negative effects of industrialized agricultural production (e.g., by limiting pesticide exposure to honey bees), they also provide a “fix” that supports and expands industrial agriculture by stabilizing managed bee pollination services. Increasing reliance on managed bee pollination services can thus disincentivize transitions to sustainable food production, such as adopting diversified practices that would support native bee populations and reduce the need for managed honey bees on farms.

Integrated Pest Management Control of Varroa destructor (Acari: Varroidae), the Most Damaging Pest of (Apis mellifera L. (Hymenoptera: Apidae)) Colonies.

Varroa destructor is among the greatest biological threats to western honey bee (Apis mellifera L.) health worldwide. Beekeepers routinely use chemical treatments to control this parasite, though overuse and mismanagement of these treatments have led to widespread resistance in Varroa populations. Integrated Pest Management (IPM) is an ecologically based, sustainable approach to pest management that relies on a combination of control tactics that minimize environmental impacts. Herein, we provide an in-depth review of the components of IPM in a Varroa control context. These include determining economic thresholds for the mite, identification of and monitoring for Varroa, prevention strategies, and risk conscious treatments. Furthermore, we provide a detailed review of cultural, mechanical, biological, and chemical control strategies, both longstanding and emerging, used against Varroa globally. For each control type, we describe all available treatments, their efficacies against Varroa as described in the primary scientific literature, and the obstacles to their adoption. Unfortunately, reliable IPM protocols do not exist for Varroa due to the complex biology of the mite and strong reliance on chemical control by beekeepers. To encourage beekeeper adoption, a successful IPM approach to Varroa control in managed colonies must be an improvement over conventional control methods and include cost-effective treatments that can be employed readily by beekeepers. It is our intention to provide the most thorough review of Varroa control options available, ultimately framing our discussion within the context of IPM. We hope this article is a call-to-arms against the most damaging pest managed honey bee colonies face worldwide.

MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity.

Simple SummaryThe western honey bee is one of the most economically and ecologically important species currently facing serious challenges in its whole area of distribution. The honey bee is a highly diverse species with about 30 subspecies that are adapted to regional climate factors, vegetation, pests and pathogens. The local populations of honey bees are rapidly changing and their diversity is constantly manipulated by beekeepers through the import of foreign queens, selection and migratory beekeeping. This manipulation may lead to such changes that honey bees lose their ability to thrive in the areas that were previously suitable for their wellbeing. To see how this human interference changed the genetic variability of native honey bee populations from Serbia, we sequenced part of the mitochondrial genome and compared them with published sequences. Our results suggest that human influence significantly changes the natural composition of honey bees in Serbia and that the presence of some previously reported subspecies could not be confirmed.Local populations of Apis mellifera are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial tRNAleu-cox2 intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish A. m. carnica from A. m. macedonica, both previously reported in Serbia, PCR-RFLP analysis on the COI gene segment of mtDNA was used, and the result showed only the presence of A.m. carnica subspecies. An MDS plot constructed on pairwise FST values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.

Comparison of the pollination efficiency of Apis cerana with wild bees in oil-seed camellia fields

Camellia oleifera Abel is an economically-important, cross-pollinated oil tree grown commercially in China. Fruit set is greatly enhanced when animal pollinators are available in this system. Prior studies have shown that bees are the primary pollinators of Camellia oleifera, but we do not know which are the most effective. This study was conducted in Xinyu, Jiangxi province, China. Through tracking observation, we measured visit duration and the tendency of bees to visit new plants, critical for the self-incompatible C. oleifera. Further, we assessed pollen loads, pollen removal rates and pollen deposition per single visit to C. oleifera, as measures of pollination efficiency for comparing the managed honey bee (A. cerana) with wild bees (C. gigas and four Andrena species). Determining the pollination efficiency of bees is important for increasing fruit set and crop yield.The managed hongeybee Apis cerana has the longest single-visit time (92.40±8.31s) with a higher visit frequency to flowers on the same tree. The difference in duration was not significant between Apis cerana and three wild bee species (A. camellia, A. hunanensis and A. striata). Conversely, the pollen deposition of A. cerana was the lowest, significantly lower than that of four species of Andrena. Further, there were significant differences in the pollen load of six species: the amount of pollens on hind legs and body surface of A. cerana was much lower than in the wild bees. Compared with the four species of Andrena, Colletes gigas moved between plants more frequently. Given its large size, it also had the highest pollen load size, pollen removal and higher deposition rates, meaning it can essentially deposit more pollen across more flowers per unit time. The efficiency of Andrena striata Wu was relatively low compared to its congeners, which were similar.

Diurnal and temporal activity of pronubial insects on berseem flowers in a subtropical environment

Pollination is the most important ecosystem service performed by insect pollinators benefiting more than three quarters of the major crops in the world. Conservation and management of honey bees are an essential part of the sustainable productivity of cross pollinated crops. The present study was conducted to identify the potential pollinator of berseem in Bundelkhand region, which typically represents the subtropical climatic condition. In total, 14 species of insects belonging to seven families of three insect orders were found visiting berseem flowers. The most frequent floral guests were hymenopterans (81 percent) followed by Lepidoptera (13 percent) and Diptera (6 percent) during the winter season of 2017–2018. Apis dorsata was the most dominant floral visitor, whose peak activity was observed between 10.00 AM and 11.00 AM (7.354/m2/10 min). The activities of pollinators were found maximum during the peak flowering stage of berseem. Seeds obtained from open pollinated plots showed a significant increase in yield (383.24 and 491.41 percent) over caged conditions during 2017 and 2018, respectively.

An Investigation of Honey Bee Viruses Prevalence in Managed Honey Bees (Apis mellifera and Apis cerana) Undergone Colony Decline

Objective: In the absence of known clinical symptoms, viruses were considered to be the most probable key pathogens of honey bee. Therefore, the aim of this study was to investigate the prevalence and distribution of honey bee viruses in managed Apis mellifera and Apis cerana in China. Methods: We conducted a screening of 8 honey bee viruses on A. mellifera and A. cerana samples collected from 54 apiaries from 13 provinces in China using RT-PCR. Results: We found that the types and numbers of viral species significantly differed between A. mellifera and A. cerana. Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Apis mellifera filamentous virus (AmFV), and Kakugo virus (DWV-A/KV) were the primary viruses found in A. mellifera colonies, whereas Chinese Sacbrood Bee Virus (CSBV) and Sacbrood Bee Virus (SBV) were the primary viruses found in A. cerana. The percentage infection of BQCV and CSBV were 84.6% and 61.6% in all detected samples. We first detected the occurrences of Varroa destructor virus-1 (VDV-1 or DWV-B) and DWV-A/KV in China but not ABPV in both A. mellifera and A. cerana. Conclusion: This study showed that BQCV and CSBV are the major threat to investigated A. mellifera and A. cerana colonies.

Conservation genomics reveals pesticide and pathogen exposure in the declining bumble bee Bombus terricola.

In recent years, many pollinators have experienced large population declines, which threaten food security and the stability of natural ecosystems. Bumble bees are particularly important because their ability to “buzz” pollinate and tolerate cooler conditions make them critical pollinators for certain plants and regions. Here, we apply a conservation genomics approach to study the vulnerable Bombus terricola. We sequenced RNA from 30 worker abdomens, 18 of which were collected from agricultural sites and 12 of which were collected from nonagricultural sites. We found transcriptional signatures associated with exposure to insecticides, with gene expression patterns suggesting that bumble bees were exposed to neonicotinoids and/or fipronil—two compounds known to negatively impact bees. We also found transcriptional signatures associated with pathogen infections. In addition to the transcriptomic analysis, we carried out a metatranscriptomic analysis and detected five pathogens in the abdomens of workers, three of which are common in managed honey bee and bumble bee colonies. Our conservation genomics study provides functional support for the role of pesticides and pathogen spillover in the decline of B. terricola. We demonstrate that conservation genomics is an invaluable tool which allows researchers to quantify the effects of multiple stressors that impact pollinator populations in the wild.

Beescape: Characterizing user needs for environmental decision support in beekeeping

Pollinators, particularly managed honey bees, are crucial for global food systems. However, declines in populations of both wild and managed pollinators have been reported across the world. In the United States, approximately 30% of managed honey bee colonies die each year. The factors underlying these losses are well understood and include reductions in the abundance and diversity of flowering plants that pollinators depend on for food, increased insecticide use, and reduced nesting habitat for wild bees. However, translating pollinator research findings into actionable knowledge for beekeepers presents a sizable spatial decision support challenge. In this work we evaluate the utility and usability of a prototype system called Beescape which intends to support environmental decision-making for beekeepers and other stakeholders. Beescape includes tools for exploring and visualizing maps that link to modeled environmental factors that impact managed bees, including honey bees, and wild bee health. Thirty beekeepers were recruited to take part in an online user study that included task analysis and survey components to elicit user input on areas of improvement for future Beescape development. The results of our evaluation of usability and utility metrics for Beescape highlighted the need for spatially-specific foraging information, better descriptions of modeled habitat quality measures, and actionable guidance to help them manage their hives. The results of our study highlight the challenges and opportunities associated with providing decision support systems that attempt to translate emerging environmental science for audiences that may be motivated by a common goal to improve honey bee survival, but who have a diverse range of technical backgrounds, applied practices, and reasons for their interest.

PRODUCTION AND MANAGEMENT OF HONEY BEE IN DANG DISTRICT OF NEPAL

This paper studied the production and management of honey bee in dang district. 35 respondent rearing commercially honey bee of Tulsipur and Ghorahi sub-metropolitan city and Banglachuli rural municipality were selected by using Purposive sampling techniques out of 141 commercial bee growers (Registered AKC, PMAMP). Structure questionnaire where designed to sample opinion of respondents. Data were collected using M-water surveyor mobile Application by using pretesting questionnaire and analyzed using MS-Excel, Statistical Package of Social Science. Results obtained that 72% respondents commercially rearing Apis millifera and 11% rearing Apis cerana. Farmers having 22-117 numbers of hives found maximum (77%). Maximum number of hives rearing found was 500 by commercial bee keepers. Average hive number and Average productivity found were 98 and 31.2kg per hive per year (Apis millifera). 89% respondent were found increasing their enterprise and good source of income was found as the main reason to increase their enterprise whereas 11% respondent were decreasing their enterprise and Shortage of forage was found as the main reason to decrease their enterprise. Hive cleaning practice was adopted by majority of the farmer (40%) to protect the hive from rainy and summer season whereas feeding with sugar syrup was adopted by most of the farmer (80%) during rainy and summer season. Hive covering with jute sacs and straw was found most adopted practice (69%) to protect the bee colonies during winter season. Commercial bee keepers having more number of bee hives and migrate their beehives for foraging in different places outside the valley were found 4 and more times honey harvesting whereas two times harvesting was found by beekeepers rearing Apis cerana. Most of the farmer used to sell honey from farm gate (94%) however the most selling was done to the whole sellers due to lack of storing and processing equipment.

Using Citizen Science to Scout Honey Bee Colonies That Naturally Survive Varroa destructor Infestations.

Simple SummaryCitizen Science is a valuable resource that can substantially contribute to the conservation of biodiversity. However, its use in honey bee research has remained minimal. The Survivors Task Force of the COLOSS association created and promoted an online surveying tool with the aim of identifying potential cases of Western honey bee, Apis mellifera, populations that are surviving infestations with ectoparasitic mites Varroa destructor without control measures by beekeepers. The reports suggest that there could be twice as many naturally surviving colonies worldwide than are currently known. The survey also shows that citizens can be readily engaged through social media, personal networks, and promotional campaigns to gather valuable and previously inaccessible data. These reports of surviving honey bee colonies will now be validated through the new initiative Honey Bee Watch, a global and multi-year Citizen Science project founded to connect citizens, beekeepers, and scientists. This will enable to increase scientific knowledge, mitigate honey bee colony losses, and develop education and conservation campaigns.Citizen Science contributes significantly to the conservation of biodiversity, but its application to honey bee research has remained minimal. Even though certain European honey bee (Apis mellifera) populations are known to naturally survive Varroa destructor infestations, it is unclear how widespread or common such populations are. Such colonies are highly valuable for investigating the mechanisms enabling colony survival, as well as for tracking the conservation status of free-living honey bees. Here, we use targeted Citizen Science to identify potentially new cases of managed or free-living A. mellifera populations that survive V. destructor without mite control strategies. In 2018, a survey containing 20 questions was developed, translated into 13 languages, and promoted at beekeeping conferences and online. After three years, 305 reports were collected from 28 countries: 241 from managed colonies and 64 from free-living colonies. The collected data suggest that there could be twice as many naturally surviving colonies worldwide than are currently known. Further, online and personal promotion seem to be key for successful recruitment of participants. Although the survivor status of these colonies still needs to be confirmed, the volume of reports and responses already illustrate how effectively Citizen Science can contribute to bee research by massively increasing generated data, broadening opportunities for comparative research, and fostering collaboration between scientists, beekeepers, and citizens. The success of this survey spurred the development of a more advanced Citizen Science platform, Honey Bee Watch, that will enable a more accurate reporting, confirmation, and monitoring of surviving colonies, and strengthen the ties between science, stakeholders, and citizens to foster the protection of both free-living and managed honey bees.

Pollen source preferences and pollination efficacy of honey bee, Apis mellifera (Apidae: Hymenoptera) on Brassica napus crop

Brassica napus is an insect-pollinated crop species and offers itself as the main nutrient source to many insects that consume floral nectar. This study was carried out to ascertain the pollen preferences of Apis mellifera among the available floral resources. We wanted to identify and quantify all types of pollen collected by honeybee during the flowering period. Moreover, the foraging rate and pollination efficacy of managed honey bee colonies (placed at 250 and 500 m distance away) on B. napus crop were determined. The results revealed that A. mellifera foraged efficiently on 18 plants species belonging to 11 families during the flowering period of the Brassica crop. The Asteraceae family was represented by six plants species as pollen sources followed by Solanaceae, Malvaceae, Fabaceae, and Rosaceae represented by two plant species from each family. One floral source included Brassicaceae, Convolvulaceae, and Poaceae families. Among 18 identified plant species, 6 were weeds, 4 herbs, 4 shrubs, and 2 species each were from crops and ornamental plants. In this study, weeds were reported as the major bee supporting bee flora followed by shrub and crops. The identified pollen grains had different morphology such as sub-spheroid, prolate shape, spheroid, ovate, glandular, triangular, round, and oval shape. The maximum foraging activities of bees on B. napus took place during day hours particularly at 12:00 PM followed by 14:00 PM, and then at 10:00 AM weekly. The study revealed that the total number of pods per plant, total number of seeds per 100 pods, and weight of seeds per 10 plants were significantly higher in bee-pollinated flowering plants compared to flowering plants with no bee-pollination.

Hoary Squash Bees (Eucera pruinosa: Hymenoptera: Apidae) Provide Abundant and Reliable Pollination Services to Cucurbita Crops in Ontario (Canada)

The increasing demand for insect-pollinated crops highlights the need for crop pollination paradigms that include all available pollinators. In North America, Cucurbita crops (pumpkin, squash) depend on both wild (solitary and Bombus spp.: Hymenoptera: Apidae) and managed honey bees (Apis mellifera L. 1758: Hymenoptera: Apidae) for pollination. Temporal and spatial differences in abundance may determine which bee taxa are the most important pollinators of Cucurbita crops. We surveyed bees visiting Cucurbita crop flowers on 19 farms over four years (2015-2018) during the crop flowering period (July 1-August 30 from 06:00-12:00). All the farms surveyed had hoary squash bees (Eucera pruinosa (Say, 1867), and most also had some combination of honey bees, bumble bees (Bombus spp.), or other wild bees present on their Cucurbita crop flowers. All four bee taxa were present on about two-thirds of farms. Spatially and temporally, wild bees were more abundant on Cucurbita crop flowers than managed honey bees. Hoary squash bees were the most abundant wild bees, maintaining their abundance relative to other wild bee taxa year-over-year. Male hoary squash bees were both more frequently and consistently seen visiting crop flowers than females in all years. Peak activity of hoary squash bees and bumble bees coincided with the daily crop pollination window, whereas peak activity of honey bees and other wild bees occurred after that window. In addition to elucidating the ecological interactions among wild and managed pollinators on Cucurbita crops, our work provides a novel practical way to evaluate pollinator abundance using a crop-centered benchmark framework.