Honey Bee Larvae Research Articles

Combined transcriptome and metabolite profiling analyses provide insights into the chronic toxicity of carbaryl and acetamiprid to Apis mellifera larvae

Despite many studies have revealed that developing honey bee (Apis mellifera) larvae are posting a high risk on exposure to insecticides, the toxicology information on bee larvae remain limited. The present study demonstrated the first assessment of the effects of no observed adverse effect concentration (NOAEC) of carbaryl (CR) and acetamiprid (ACE) on transcriptome and metabolome in honeybee larvae reared in vitro. Chronic exposure to carbaryl caused transcriptional disorders associated with oxidative stress. In addition, a series of metabolic homeostasis were disrupted by carbaryl stress, such amino acid metabolism, purine and pyrimidine metabolism and flavone and flavonol biosynthesis. The activities of enzymic biomarkers including GST, P450, CAT, AChE and SOD were not influenced by ACE stress, while the CR exposure slightly decreased the activity of CAT and SOD. Our results clearly show that ACE and CR display different potential to modulate transcriptome and metabolome associated with their different toxicity against bee larvae.

Evaluation of the Biological Activities of Royal Jelly on Prostate and Breast Cancer Cells

Due to their high toxicity to healthy body cells, chemotherapy drugs used to treat cancer pose a serious threat to the organism. Recent studies have encouraged the utilization of bee products to prevent and treat the cancer. The specific food for the queen honeybee larva (Apis mellifera), royal jelly (RJ) is a yellow, milky, cremy product of the bee which is stowed from the mandibular and hypopharyngeal glands of the worker honeybee. According to the reports, RJ may have cytotoxic effects in animal models. However, RJ's cytotoxic and antioxidant properties on PC-3 prostate cancer and MCF-7 breast cancer cell lines have yet to be thoroughly investigated at the cellular level. Therefore, the antioxidant and anti-proliferative activities of RJ in PC-3 prostate cancer cells and MCF-7 breast cancer cells were evaluated to investigate a chemopreventive strategy to increase cancer therapy efficacy. WST-1 assay was used to measure cell proliferation, and levels of reactive oxygen species (ROS) and lipid peroxidation (LPO) were analyzed to look at antioxidant activities. According to the data of this research, RJ act as prooxidants in PC-3 and MCF-7 cancer cell lines by lessening cellular viability and raising ROS and LPO formation.

Improvement of Self-Perceived Tinnitus and Speech Discrimination in Noise by Lyophilized Powder of Enzymolyzed Honeybee (Apis melifera) Larvae in Older Adults with Age-Related Hearing Loss: A Double-Blind Placebo-Controlled, Randomized Study

Background: Tinnitus troubles many older adults accompanying an increased threshold of high-frequency hearing, and most older adults struggle to understand conversations in noise. Objectives: An exciting power of honeybee products has been reported as alternative medicine. We also previously reported that lyophilized powder of enzymolyzed honeybee (Apis melifera) larvae may have a therapeutic effect on hearing loss or tinnitus.

Assessment of acute and chronic toxicity of cyantraniliprole and sulfoxaflor on honey bee (Apis mellifera) larvae.

Recently, cyantraniliprole (CYA) and sulfoxaflor (SUL) have been considered as alternatives to neonicotinoid insecticides. In this study, we evaluated the acute and chronic toxicities of CYA and SUL on honey bee (Apis mellifera L.) larvae reared in vitro. In the acute toxicity test, the following test doses were used to determine the median lethal dose (LD50 ): CYA 0.007, 0.014, 0.028, 0.056 and 0.112 μg larva-1 ; SUL 2.5, 5, 10, 20 and 40 μg larva-1 . In the chronic toxicity test, the following test doses were used to determine the LD50 : CYA 0.00512, 0.0128, 0.032, 0.08 and 0.2μg larva-1 ; SUL 0.0625, 0.125, 0.25, 0.5 and 1.0μg larva-1 . The acute LD50 values of CYA and SUL were 0.047 and 11.404 μg larva-1 , respectively. Larvae acutely exposed to SUL had significantly lower body weight than controls, but those exposed to CYA showed no difference. The no observed adverse effect level (NOAEL) and LD50 values of the chronic toxicity tests for each insecticide were 0.00512 and 0.064 μg larva-1 for CYA, and 0.0625 μg larva-1 and 0.212 μg larva-1 for SUL, respectively. Larvae chronically exposed to SUL emerged as bees with deformed wings, reaching adult deformation rates of over 50%; however, CYA had no effect on adult deformation. Exposure to CYA increased larval mortality but did not cause any adult deformation, whereas SUL exposure increased pupal mortality and caused wing deformation in newly emerged bees. Our study may be useful for the assessment of pesticide toxicity by providing valuable findings on the effects of these insecticides on honey bee larvae. © 2022 Society of Chemical Industry.

Extremely Low-Frequency Electromagnetic Field Impairs the Development of Honeybee (Apis cerana).

Simple SummaryThe ELF-EMF pollution generated by the increase in electrically powered devices and power lines, accompanied by economic development, has a widespread effect on surrounding organisms. Honeybees are one of the most important pollinators. The decline in the honeybee population caused by a variety factors, including EMFs, has attracted attention worldwide. It was already known that ELF-EMFs could impair the ability of learning and cognition, causing foraging bees to lose their ability to find their way home. The pollination ability of foraging bees is derived from the rearing quantity of larvae and continuous eclosion of new adult bees in the colony. However, the effect of ELF-EMFs on honeybee larvae is not clear. The aims and objectives of this study were therefore to investigate it. The results showed that ELF-EMF exposure decreases honeybee survival rate and body weight and extends the duration of development time. Transcriptome sequencing showed that ELF-EMF exposure decreases the biological process of nutrient and energy metabolism, impedes the degradation of larvae tissues and the rebuilding of pupae tissues in the metamorphosis process, and seriously interferes with the growth and development of honeybee larvae. This provides an experimental basis and new perspective for protecting honeybee populations from ELF-EMF pollution.Increasing ELF-EMF pollution in the surrounding environment could impair the cognition and learning ability of honeybees, posing a threat to the honeybee population and its pollination ability. In a social honeybee colony, the numbers of adult bees rely on the successful large-scale rearing of larvae and continuous eclosion of new adult bees. However, no studies exist on the influence of ELF-EMFs on honeybee larvae. Therefore, we investigated the survival rate, body weight, and developmental duration of first instar larvae continuously subjected to ELF-EMF exposure. Moreover, the transcriptome of fifth instar larvae were sequenced for analyzing the difference in expressed genes. The results showed that ELF-EMF exposure decreases the survival rate and body weight of both white-eye pupae and newly emerged adults, extends the duration of development time and seriously interferes with the process of metamorphosis and pupation. The transcriptome sequencing showed that ELF-EMF exposure decreases the nutrient and energy metabolism and impedes the degradation of larvae tissues and rebuilding of pupae tissues in the metamorphosis process. The results provide an experimental basis and a new perspective for the protection of honeybee populations from ELF-EMF pollution.

Infestation of <em>Apis mellifera</em> workers and larvae with <em>Varroa destructor</em> affects gut bacterial diversity

The present work aims to investigate the midgut bacteria of honey bee (Apis mellifera lamarckii) workers and larvae infested with Varroa destructor. To achieve this task, uninfested and infested honey bee larvae and workers with the parasitic mites were obtained from natural bees apiaries in Qaliubiya Governorate, Egypt. Aerobic bacteria were isolated from the midgut of uninfested and infested A. mellifera on nutrient agar medium and identified using a culture-dependent isolation along with 16S rRNA sequences. The results revealed that both uninfested and V. destructor infested workers and larvae harbored gut bacteria belonging to Gamma-proteobacteria strains. The uninfested workers contained Enterobacter cloacae and Serratia nematodiphila, while V. destructor infested workers harbored Enterobacter species. Uninfested larvae contained E. hormaechei and Klebsiella variicola. V. destructor infested larvae harbored K. oxytoca as a result of infection. We concluded that the honeybee microbiome composition is likely to be impacted through isolates variation by stage and diversity disruption of isolates by V. destructor infection.

Effect Of Fenvalerate, λ-cyhalothrin, Quinalphos And Thiamethoxam On Larval Survival In Honey Bee <i>Apis mellifera</i> L.

This study evaluates the effects of some insecticides on the survival of larvae of Apis mellifera L. The pyrethroids (fenvalerate and λ-cyhalothrin) caused maximum mortality at highest concentration (12.5 ppm), when compared to quinalphos and thiamethoxam. Fenvalerate was observed to be extremely toxic in its maximum concentration, as none among 1-2 days old treated larvae (after multiple exposures) survived after 24 hours (i.e., after 4<sup>th</sup> exposure. Lavae were observed to be tolerant to thiamethoxam as 66.67% survival was observed till emergence under similar conditions.

Simulating the Effects of Pesticides on Honey Bee (Apis mellifera L.) Colonies with BeePop+

The US Environmental Protection Agency (USEPA) employs a tiered process for assessing risks of pesticides to bees. The model discussed in this paper focuses on honey bees (Apis mellifera L.). If risks to honey bees are identified at the first tier based on exposure and toxicity data for individual adult and larval honey bees, then effects are evaluated in higher-tier studies using honey bee colonies. Colony-level studies require large amounts of resources (to conduct and review) and can yield data complicated by the inherent variability of colonies, which are influenced by factors that cannot readily be controlled, including weather, pests, diseases, available forage, and bee management practices. To better interpret these data, the USEPA and the US Department of Agriculture (USDA) developed a simulation model, BeePop+, that assesses potential honey bee colony-level effects of pesticides. Here, we describe this model using the population model guidance, use, interpretation, and development for ecological risk assessment (Pop-GUIDE) framework, which is a conceptual framework for the development and evaluation of population models. Within the context of Pop-GUIDE, BeePop+ is considered a “realistic-precise” model and reflects the inherent variability of colony response to pesticide exposure by simulating many outcomes. This model meets the desired features needed for use in pesticide risk assessments as its required data inputs are typically available, it is applicable to different US locations, and the outputs are both relevant to USEPA’s protection goals for honey bees and are consistent with the outcomes of empirical studies. This model has also been evaluated using available empirical colony-level data; however, additional evaluation with other studies may still be done in the future prior to completing implementation.

Volatile biomarkers for non-invasive detection of American foulbrood, a threat to honey bee pollination services

Honey bees provide essential environmental services, pollinating both agricultural and natural ecosystems that are crucial for human health. However, these pollination services are under threat by outbreaks of the bacterial honey bee disease American foulbrood (AFB). Caused by the bacterium, Paenibacillus larvae, AFB kills honey bee larvae, converting the biomass to a foul smelling, spore-laden mass. Due to the bacterium's tough endospores, which are easily spread and extremely persistent, AFB management requires the destruction of infected colonies in many countries. AFB detection remains a significant problem for beekeepers: diagnosis is often slow, relying on beekeepers visually identifying symptoms in the colony and molecular confirmation. Delayed detection can result in large outbreaks during high-density beekeeping pollination events, jeopardising livelihoods and food security. In an effort to improve diagnostics, we investigated volatile compounds associated with AFB-diseased brood in vitro and in beehive air. Using Solid Phase Microextraction and Gas Chromatography Mass-Spectrometry, we identified 40 compounds as volatile biomarkers for AFB infections, including 16 compounds previously unreported in honey bee studies. In the field, we detected half of the biomarkers in situ (in beehive air) and demonstrated their sensitivity and accuracy for diagnosing AFB. The most sensitive volatile biomarker, 2,5-dimethylpyrazine, was exclusively detected in AFB-disease larvae and hives, and was detectable in beehives with <10 AFB-symptomatic larvae. These, to our knowledge, previously undescribed biomarkers are prime candidates to be targeted by a portable sensor device for rapid and non-invasive diagnosis of AFB in beehives.

Honey bee symbiont buffers larvae against nutritional stress and supplements lysine.

Honey bees have suffered dramatic losses in recent years, largely due to multiple stressors underpinned by poor nutrition [1]. Nutritional stress especially harms larvae, who mature into workers unable to meet the needs of their colony [2]. In this study, we characterize the metabolic capabilities of a honey bee larvae-associated bacterium, Bombella apis (formerly Parasaccharibacter apium), and its effects on the nutritional resilience of larvae. We found that B. apis is the only bacterium associated with larvae that can withstand the antimicrobial larval diet. Further, we found that B. apis can synthesize all essential amino acids and significantly alters the amino acid content of synthetic larval diet, largely by supplying the essential amino acid lysine. Analyses of gene gain/loss across the phylogeny suggest that four amino acid transporters were gained in recent B. apis ancestors. In addition, the transporter LysE is conserved across all sequenced strains of B. apis. Finally, we tested the impact of B. apis on developing honey bee larvae subjected to nutritional stress and found that larvae supplemented with B. apis are bolstered against mass reduction despite limited nutrition. Together, these data suggest a novel role of B. apis as a nutritional mutualist of honey bee larvae.

Adenosine and L-proline can possibly hinder Chinese Sacbrood virus infection in honey bees via immune modulation

Sacbrood virus (SBV) infects larvae of honey bees, resulting in infected larvae becoming fluid-filled sacs. Our previous studies showed that the extract of herbal medicine, Radix Isatidis, could inhibit Chinese SBV (CSBV) infection in Asian honey bees (Apis cerana). Here, two compounds, adenosine and L-proline, which were previously reported to be associated with immune modulation, were identified in R. Isatidis extract and then selected for an evaluation of their antiviral effect on CSBV infection in A. cerana. Our results revealed that both adenosine and L-proline could significantly mitigate the impact of CSBV on the growth and development of infected larvae and modulate hosts' immune responses by downregulating the expression of immune genes in infected larvae. The results gained from this study suggest that adenosine and L-proline could possibly interfere CSBV infection via immune modulation to avoid exacerbations and nonspecific damage to infected larvae's own tissues.

Interaction between Thiamethoxam and Deformed Wing Virus Type A on Wing Characteristics and Expression of Immune and Apoptosis Genes in Apis mellifera.

Simple SummaryHoney bees are key pollinators in agricultural crops. Today, honey bee colonies in decline are a global concern as a result of various stressors, including pesticides, pathogens, honey bee health, and parasites. A healthy honey bee colony refers to colonies that are not exposed to biotic and abiotic stressors. In this study, we examine how thiamethoxam (pesticide) and deformed wing virus type A (DWV-A) interact in effects on honey bee health. The results revealed that the honey bees were infected with DWV-A and were additionally exposed to thiamethoxam, showing effects that increased the mortality rate, and crippled wings in newly emerged adult honey bees. Moreover, the exposure to thiamethoxam and DWV-A injection resulted in induced expression of immune genes (hymenoptaecin gene) while downregulation of two apoptosis genes (caspase8-like, caspase9-like genes). The impact interaction of pesticide and DWV-A have on the expression of apoptosis genes can directly affect viral susceptibility in the honey bee host.Honey bees are economically important insects for crop pollination. They play a significant role as pollinators of wild plants and agricultural crops and produce economical products, such as honey, royal jelly, wax, pollen, propolis, and venom. Despite their ecological and economical importance, the global honey bee population is in decline due to factors including pathogens, parasites, intensive agriculture, and pesticides. Moreover, these factors may be interlinked and exacerbate the loss of honey bees. This study aimed to investigate the interaction between a pesticide, thiamethoxam, and deformed wing virus type A (DWV-A) to honey bees and the effects on survival rate, wing characteristics, and expression of immune and apoptosis genes in Apis mellifera. We described the potential interaction between thiamethoxam and DWV-A on honey bee wing characteristics, DWV-A loads, and the expressions of immune (defensin, abaecin, and hymenoptaecin) and apoptosis genes (buffy, apaf1, caspase3-like, caspase8-like, and caspase9-like). Honey bee larvae were fed with three different thiamethoxam doses (0.001, 1.4, and 14.3 ng/µL of the diet). Then, thiamethoxam-treated white-eyed pupae were injected with 107 copy numbers/honey bee of the DWV-A genome. The interaction between thiamethoxam and DWV-A caused a high mortality rate, crippled wings in newly emerged adult honey bees (100%), and resulted in induced expression of hymenoptaecin gene compared to the control group, while downregulation of caspase8-like, caspase9-like genes compared to the DWV injection group. Therefore, the potential interaction between thiamethoxam and DWV-A might have a deleterious effect on honey bee lifespan. The results from this study could be used as a tool to combat DWV-A infection and mitigate pesticide usage to alleviate the decrease in the honey bee population.

Sex‐ and caste‐specific transcriptomes of larval honey bee (Apis mellifera L.) gonads: DMRT A2 and Hsp83 are differentially expressed and regulated by juvenile hormone

The gonads of honey bee, Apis mellifera, queens and drones are each composed of hundreds of serial units, the ovarioles and testioles, while the ovaries of the adult subfertile workers consist of only few ovarioles. We performed a comparative RNA-seq analysis on early fifth-instar (L5F1) larval gonads, which is a critical stage in gonad development of honey bee larvae. A total of 1834 genes were identified as differentially expressed (Padj < 0.01) among the three sex and caste phenotypes. The Gene Ontology analysis showed significant enrichment for metabolism, protein or ion binding, and oxidoreductase activity, and a KEGG analysis revealed metabolic pathways as enriched. In a principal component analysis for the total transcriptomes and hierarchical clustering of the DEGs, we found higher similarity between the queen and worker ovary transcriptomes compared to the drone testis, despite the onset of programmed cell death in the worker ovaries. Four DEGs were selected for RT-qPCR analyses, including their response to juvenile hormone (JH), which is a critical factor in the caste-specific development of the ovaries. Among these, DMRT A2 and Hsp83 were found upregulated by JH and, thus, emerged as potential molecular markers for sex- and caste-specific gonad development in honey bees.

Short Communication: Efficacy of Two Commercial Disinfectants on Paenibacillus larvae Spores.

Paenibacillus larvae is a spore-forming bacterium causing American foulbrood (AFB) in honey bee larvae. The remains of a diseased larva contains billions of extremely resilient P. larvae spores viable for decades. Burning clinically symptomatic colonies is widely considered the only workable strategy to prevent further spread of the disease, and the management practices used for decontamination requires high concentrations of chemicals or special equipment. The aim of this study was to test and compare the biocidal effect of two commercially available disinfectants, “Disinfection for beekeeping” and Virkon S on P. larvae. The two products were applied to P. larvae spores in suspension as well as inoculated on two common beehive materials, wood and Styrofoam. “Disinfection for beekeeping” had a 100 % biocidal effect on P. larvae spores in suspension compared to 87.0–88.6% for Virkon S which, however, had a significantly better effect on P. larvae on Styrofoam. The two disinfectants had similar effect on infected wood material.

Juvenile Hormone III R Stereoisomer Is Specifically Synthesized by Honeybees (Apis mellifera ligustica) and Shows a Higher Biological Activity in Regulating Their Social Behavior.

The juvenile hormone (JH) plays a key role in the development of honeybee larvae and the alteration of adult behavior. Verification methods of types and stereoisomers of JHs in honeybees were established in this study. The regulatory modes of different stereoisomers of JH III on the social behaviors of honeybees were revealed by the disparity of interaction and RNA-seq. This result represented the first assessment of the effects of R-JH III and S-JH III in honeybee interactions; the former (367 times in total) was significantly higher than the latter (235 times in total); honeybees with high JH titers are always welcome in the colony because the effect of JH III on bees involves the sensing and signaling of hormones, and R-JH III is much more active than S-JH III in this regulation. Efficient R-JH III may be the insurance for bees to establish their social system advantages.

Lack of evidence for trans-generational immune priming against the honey bee pathogen Melissococcus plutonius.

Trans-generational immune priming involves the transfer of immunological experience, acquired by the parents after exposure to pathogens, to protect their progeny against infections by these pathogens. Such natural mechanisms could be exploited to prevent disease expression in economically important insects, such as the honey bee. This mechanism occurs when honey bee queens are exposed to the pathogenic bacterium Paenibacillus larvae. Here, we tested whether natural or experimental exposure to Melissococcus plutonius—another bacterium triggering a disease in honey bee larvae—reduced the susceptibility of the queen’s progeny to infection by this pathogen. Because the immunological response upon pathogen exposure can lead to fitness costs, we also determined whether experimental exposure of the queens affected them or their colony negatively. Neither natural nor experimental exposure induced protection in the honey bee larvae against the deleterious effects of M. plutonius. Our results provided no evidence for the occurrence of trans-generational immune priming upon exposure of the queen to M. plutonius. Whether this lack was due to confounding genetic resistance, to unsuitable exposure procedure or to the absence of trans-generational immune priming against this pathogen in honey bees remains to be determined.

Physicochemical properties, molecular structure, antioxidant activity, and biological function of extracellular melanin from Ascosphaera apis.

Ascosphaera apis spores containing a dark-colored pigment infect honeybee larvae, resulting in a large-scale collapse of the bee colony due to chalkbrood disease. However, little is known about the pigment or whether it plays a role in bee infection caused by A. apis. In this study, the pigment was isolated by alkali extraction, acid hydrolysis, and repeated precipitation. Ultraviolet (UV) analysis revealed that the pigment had a color value of 273, a maximum absorption peak at 195 nm, and a high alkaline solubility (7.67%) and acid precipitability. Further chemical structure analysis of the pigment, including elemental composition, Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, mass spectrometry, and nuclear magnetic resonance (NMR), proved that it was a eumelanin with a typical indole structure. The molecular formula of melanin is C10H6O4N2, and its molecular weight is 409 Da. Melanin has hydroxyl, carboxyl, amino, and phenolic groups that can potentially chelate to metal ions. Antioxidant function analyses showed that A. apis melanin had a high scavenging activity against superoxide, hydroxyl, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and a high reducing ability to Fe3+. Indirect immunofluorescence assay (IFA), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analyses showed that A. apis melanin was located on the spore wall. The spore wall localization, antioxidant activity, and metal ion chelating properties of fungal melanin have been suggested to contribute to spore pathogenicity. However, further infection experiments showed that melanin-deficient spores did not reduce the mortality of bee larvae, indicating that melanin does not increase the virulence of A. apis spores. This study is the first report on melanin produced by A. apis, providing an important background reference for further study on its role in A. apis.

The influence of an analog of juvenile hormone on the silk gland of Africanized Apis mellifera larvae reared in vitro

As the larval silk glands of Africanized honey bees are well characterized in hive conditions, we compared the silk glands of larvae of Africanized honey bees from the hive (in vivo - literature information) with larvae reared under laboratory conditions according to a specific bioassay (in vitro rearing of Africanized honey bees). We also continuously exposed the larva to a juvenile hormone analog (JHA), the pyriproxyfen, in three different concentrations: 12.5 mg/L, 25 mg/L, and 50 mg/L. Concerning morphological and histochemical analyses, there were no characteristics in glands that indicated the acceleration of the cocoon spinning time and no features of cell death. The glands seemed to be in secretory activity. Pyriproxyfen-treated larvae had a slightly shorter silk synthesis period than control, although only in the 50 mg/L experimental group, the tactoids from silk were clearly visible in optical microscopy, probably due to a higher secretion activity as the response to JHA. We observed that the presence of lipids under artificial feeding was independent of the nurse bee presence. Thus, we found that pyriproxyfen alters the functioning of the silk gland, under laboratory conditions in morphological, histological, and/or morphometric aspects.

Effects of Three Types of Pollen on the Growth and Development of Honey Bee Larvae (Hymenoptera, Apidae)

Pollen serves as an essential protein source for honey bee larvae. The nutrients in pollen greatly influence larval growth and development. Here, the survival, prepupal weight, developmental stage, pollen digestibility and midgut cells in honey bee (Apis mellifera L.) larvae were evaluated by performing in vitro and 5-ethynyl-2′-deoxyuridine (EdU) assays on larvae reared on three single pollens (Brassica napus L., Armeniaca sibirica L., and Pyrus bretschneideri Rehd.) and a pollen mixture (mixture of the three pollens in equal proportions). The results showed that the survival rate of larvae fed 10 mg of rape pollen was lowest (P &amp;lt; 0.05), but there were no notable differences in the survival rate among the groups receiving the other types and doses of pollen (P &amp;gt; 0.05). The prepupal weight of larvae fed apricot pollen was significantly lower than those of the other groups (P &amp;lt; 0.05). The digestibility of rape pollen and the pollen mixture were dramatically higher than those of apricot and pear pollen (P &amp;lt; 0.05). Pear and mixed pollen exerted negative effects on the nuclear area of midgut cells in the early larval stage (P &amp;lt; 0.05). In conclusion, detection of larval midgut cells using the EdU assay might be an effective method to assess the pollen nutritive value in honey bees. Compared to apricot and pear pollen, rape pollen was more beneficial in larval honey bee growth and development.

A retrospective analysis of honey bee (Apis mellifera) pesticide toxicity data.

Current USEPA ecological risk assessments for pesticide registration include a determination of potential risks to bees. Toxicity data are submitted to support these assessments and the USEPA maintains a large database containing acute and chronic toxicity data on adult and larval honey bees (Apis mellifera), which USEPA considers a surrogate for Apis and non-Apis bees. We compared these toxicity data to explore possible trends. This analysis indicated a significant correlation between acute contact and oral median lethal dose (LD50) values for adult honey bees (ρ = 0.74, p <0.0001). Using default EPA modeling assumptions, where exposure for an individual bee is roughly 12x lower through contact than through ingestion, the analysis indicates that the oral LD50 is similarly if not more protective of the contact LD50 for the majority of pesticides and modes of action evaluated. The analysis also provided evidence that compounds with a lower acute toxicity for adults through contact and oral exposure pathways may still be acutely toxic for larvae. The acute toxicity of herbicides and fungicides was higher for larvae relative to oral and contact toxicity for adult honey bees for the same compounds and the no observed adverse effect level (NOAEL) from chronic toxicity studies were lower for larvae relative to adults, indicating increased sensitivity of larvae. When comparing 8-day LD50 values between single dose larval acute studies to those derived from repeat dose 22-day larval chronic toxicity studies, the LD50 values derived from chronic studies were significantly lower than those from acute toxicity tests (Z = -37, p = 0.03).