The Vibrionales are widespread, free-living, Gram-negative proteobacteria (22) that have been linked to pathogenicity in animals and gastroenteric infection in humans. We report the annotated genome sequence of a free-living strain of Vibrio furnissii (NCTC 11218) harvested from an estuarine environment. It consists of two circular chromosomes (3.2 Mb and 1.6 Mb) and reveals novel genes likely to be involved in pathogenicity. Interest in the Vibrio is stimulated mainly by the association of certain species with human and animal diseases (1, 4, 5, 6, 8, 20) and by quorum-derived bioluminescent symbiosis with marine animals (3, 24). The most intensively studied species, V. cholerae, can be lethally pathogenic (20). To date, 10 noncholeric Vibrio species have been implicated in human infection (11). These “emerging Vibrio species” include V. furnissii, a widespread, free-living, marine species that is associated with acute gastroenteritis (5, 9). More recently, V. furnissii has been reported to produce significant amounts of hydrocarbons that can be converted into renewable biofuels (18, 23). The complete genome sequence of V. furnissii strain NCTC 11218 was determined using shotgun and pyrosequencing, at Agencourt Biosciences (Beckman Coulter Genomics). V. furnissii was sourced from the National Collection of Type Cultures (Health Protection Agency, United Kingdom) (13). Open reading frame (ORF) prediction was performed using GLIMMER software (10). The genome was scanned for tRNAs and tmRNAs by using tRNAscan-SE 1.23 and ARAGON, respectively (12, 14). The assembled V. furnissii genome comprises two circular chromosomes. Chromosome I is 3,294,546 bp, and chromosome II is 1,621,862 bp. The purine content of chromosome I (G+Cchr.I) is 50.73%, and that of chromosome II (G+Cchr.II) is 50.54%, which is 4 to 10% higher than the other sequenced Vibrio species. Chromosome I contains 3,013 ORFs and 95 tRNA sequences, and chromosome II has 1,448 ORFs and 5 tRNA sequences, which is comparable to other Vibrio. Despite reports of pathogenicity from V. furnissii (1, 21), the genome does not contain homologues to a number of the factors associated with virulence that have been identified in other Vibrio species, such as toxRT, zot, ctxAB, and genes encoding the type III secretion system. Conversely, other sequences, including ompU, hylA, toxS, and tcpA/tcpI, are present (9, 15). However, the V. furnissii genome also contains novel sequences that may be associated with virulence. For example, VfuB00340 is an ORF encoding a 3,150-amino-acid polypeptide (329.18 kDa) with no homologues in the bacterial database. The protein is predicted to contain one signal peptide domain, 16 VCBS (hemolysin-type calcium-binding repeat) domains, and no transmembrane helix, which suggests that the gene product is a novel exoprotein that may have a role in cell adhesion and/or pathogenesis (2, 7). Therefore, despite scant genomic evidence that V. furnissii poses a significant pathogenic threat, the V. furnissii genome contains novel sequences that warrant further investigation. In terms of bioluminescence and quorum sensing, the AI-2 production gene luxS is present in the V. furnissii genome, but the transcriptional regulator luxR (17) and the luciferase genes luxAB that mediate bioluminescence (16) are absent. Concerning the recent reports of large-scale hydrocarbon production by V. furnissii, we confirm that the genome contains no obvious genes for the synthesis of alkanes (18, 19, 23).