Event Abstract Back to Event Platelets and leukocytes reactivity in patients with ischemic stroke Iryna Prylutska1*, Eduard Barinov1, Oksana Sulaieva1 and Andrey Kit1 1 Donetsk National Medical University, Ukraine The most common cause of stroke is the sudden occlusion of a blood vessel by a thrombus or embolism, associated with platelet and leukocyte dysfunction. The last one determines the induction of pro-inflammatory cascade, cerebral injury progression and complications development. The aim of this investigation was to estimate the cooperation between platelets and leukocytes in blood of patients with ischemic stroke depending on outcome. Materials and Methods. The functional state of platelets and leukocytes was estimated in 40 patients with acute ischemic stroke. The 1st group includes 30 stroke survivors, the 2nd - 10 patients with lethal outcome. We assessed platelets aggregation induced by epinephrine (2,5 μM), ADP (5 μM), thrombin (1,5 NIH) and angiotensin II (1 μM). Platelet-leukocyte aggregates (PLA) in peripheral blood were analyzed morphologically. Results. The total number of leukocytes and segmented neutrophils percentage rates in complete blood count were significantly higher in 2nd group of patients comparing with 1st group (p=0,005 and p=0,026 respectively). Pro-inflammatory activation of leukocytes was related with PLA formation and lysed leukocytes appearance, which number were significantly higher in 2nd group (p<0,001). Comparison of all agonists effects on blood cells activation shown the most prominent effect of thrombin, inducing formation of heterogeneous and homogeneous aggregates, which number was in two times higher in patients with lethal outcome comparing 1st group (p=0,001). Conclusion. Ischemic stroke is associated with pro-inflammatory activation of platelets and leukocytes, accelerated by thrombin and reflected in PLA formation. These findings can be useful in ischemic stroke outcome prognosis. Keywords: Platelet, leukocyte, ischemic stroke, aggregate, Thrombin Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Prylutska I, Barinov E, Sulaieva O and Kit A (2013). Platelets and leukocytes reactivity in patients with ischemic stroke. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00528 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Iryna Prylutska, Donetsk National Medical University, Donetsk, Ukraine, priral@bigmir.net Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Iryna Prylutska Eduard Barinov Oksana Sulaieva Andrey Kit Google Iryna Prylutska Eduard Barinov Oksana Sulaieva Andrey Kit Google Scholar Iryna Prylutska Eduard Barinov Oksana Sulaieva Andrey Kit PubMed Iryna Prylutska Eduard Barinov Oksana Sulaieva Andrey Kit Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.