Homeopathic Preparations Research Articles

Effects of homeopathic preparations of Viscum album on in vivo murine melanoma model

Recently, several authors have shown the benefits of homeopathy in improving the quality of lifeand survival of cancer patients. Preliminary data from the group showed that B16F10 murinemelanoma cells treated with Viscum album (Va), collected from the host tree Quercus robur duringthe Swiss summer (VaQr.s) and prepared in different homeopathic scales: 12 dH, 200 cH and 5 lmdecreased both viability and production of reactive oxygen species (ROS) and MCP-1 cytokine,when compared to B16F10 cells treated with the chemotherapy drug carboplatin. However, the invivo effects of these treatments have not been evaluated. Therefore, the aim of the present workwas to investigate the effects of different homeopathic preparations of VaQr.s in mice withmelanoma. For this, C57BL/6 mice were inoculated subcutaneously (s.c) on the left flank withB16F10 cells. After appearance of tumor nodule, mice were treated three times a week, orally,during 3 weeks with mother tincture (TM) or different potencies of VaQr.s (12 dH, 200 cH or5 lm). As controls, mice were treated with water or vehicle (10%-alcohol) or chemotherapy(carboplatin). Evaluations of the animals' general condition and tumor growth were performed onalternate days until approximately 30 days after tumor cell inoculation. The results obtainedshowed that mice treated with VaQr 12 dH or 5 lm have increased survival and body weight, aswell as a tendency to increase the quality of life of animals with melanoma, since these presentedless clinical manifestations when compared to the other treatments, mainly the carboplatin group.Together, the data presented here demonstrated for the first time, the effects of Va potencies onalleviating cancer-related fatigue in an in vivo model of murine melanoma. Furthermore, this workprovides relevant information for understanding the molecular events induced by homeopathicpreparations of Va as a support for future studies with ultradiluted Va

Тонзилектомія в дітей: терапевтичні можливості в оптимізації результатів хірургічного втручання

Tonsillectomy is one of the most common surgical interventions. The search for effective medical measures to optimize the course of regeneration processes in a postoperative wound is urgent. Purpose - to evaluate the effect of the complex homeopathic preparation Tonsilotren on the reparative processes of the postoperative period in children who underwent tonsillectomy. Materials and methods. The test group of 70 patients with chronic tonsillitis and 30 patients of the control group aged from 6 to 17 years were under observed and compared. All patients in the test group received Tonsilotren 7 days before surgery and for 2 weeks after surgical tonsillectomy. The examination of the ENT doctor was carried out during 5 visits: 1 visit before the operation, 2-5 visits - corresponded to the period from 1 to 14 days of the postoperative period. The results of observation were evaluated according to the following indicators: hyperemia of the mucous membrane of the faucial pillars, swelling of the mucous membrane of the faucial pillars, fibrinous pellicle in the postoperative wound, epithelization of the postoperative wound, bleeding from the postoperative wound, blood clots in the postoperative wound, scarring of the faucial pillars, pain intensity to VAS scale during swallowing and eating solid food, side effects, tolerability of the Tonsilotren medicine, complications of the disease, the need and duration of use of analgesics. Results. Prescribing of the Tonsilotren medicine to patients with chronic tonsillitis during and after surgical treatment (tonsillectomy) significantly increases the effectiveness of the treatment, namely: it helps to reduce the intensity of pain during swallowing and eating solid food, hyperemia and swelling of the mucous membrane of the faucial pillars, acceleration of epithelization of the postoperative wound, reduces scaring of faucial pillars (p<0.05). Epithelization of the postoperative wound with an area of more than 75% of its surface was observed in 24 (34.3%) patients on the 10th day and in 68 (97.1%) patients on the 14th day after the operation in the test group, while this indicator occurred in the control group only in 13 (43.3%) children on the 14th day after surgery. During observation, no side effects of the Tonsilotren medicine and adverse events were detected, which confirms the safety of its use. Conclusions. Course use of the Tonsilotren medicine in children in preparation for tonsillectomy and in the postoperative period demonstrates better effectiveness compared to standard treatment in terms of indicators of postoperative wound healing, rates of epithelization, absence of scarring and blood clots, and less pronounced pain syndrome. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.

Phytochemical Screening and Antimicrobial Activity of Homoeopathic Dilutions of Myristica sebifera against Staphylococcus aureus

Abstract Background The Anti-Microbial Resistance (AMR) has trend of increasing morbidity and mortality which affect the management of variety of infectious disorders in humans. Our skin is native to the bacterium Staphylococcus aureus, which is also a frequent source of infections in both the general population and healthcare settings. On the other side, Myristica sebifera is known for its great antiseptic activity in Homoeopathy. Objective This study was aimed to explore the phytoconstituents & antimicrobial activity of Homoeopathic preparation of Myristica sebifera 12C, 30C & 200C. Materials and Methods Myristica sebifera 12C, 30C & 200C were tested for identification of its phytoconstituents and antimicrobial activity was evaluated following Kirby-Bauer Disk Diffusion (KBDD) method according to clinical and laboratory standard (CLSIM44 A) with slight modifications. The diameter of zone of inhibition was measured and compared with Gentamicin (Positive Control) & Alcohol (62% & 91% as Negative Controls). The experiment was performed thrice to check the accuracy and reproducibility. Result Myristica sebifera 12C & 30C showed maximum zone of inhibition up to (10.33 ± 1.52 & 11.67 ± 1.52 respectively) against Staphylococcus aureus when compared to control (Alcohol 62% & 91%) as 6.00 ± 0.00. Conclusion Phytochemical studies showed presence of alkaloids and carbohydrate in Myristica sebifera 12C and 30C. The maximum percentage of zone of inhibition (94%) was found in Myristica sebifera 30C followed by Myristica sebifera 12C (72%). This antimicrobial study suggests that Myristica sebifera 12C & 30C could be helpful for treating the disorders caused by Staphylococcus aureus.

The efficacy of neutraceuticals to alleviate dog osteoarthritis symptoms, a meta-analysis of case-control trials

Osteoarthritis is a common condition in dogs. Non-steroidal anti-inflammatory drugs are currently the most widely used treatment option; however, their chronic use is associated with numerous malefic side effects, including death. There has been increased interest in alternative treatments and nutraceuticals have assumed fundamental importance. A number of individual studies and systematic reviews have been done, but no meta-analysis has been produced to date. By conducting a meta-analysis, we aimed to fill this research gap. We combined the results of 13 case placebo controlled trials in a single major study, to determine the efficacy of nutraceuticals in alleviating symptoms of osteoarthritis. A population of 638 dogs was used, from which 327 were treated with nutraceuticals and 321 were used as controls. A successful random effects model was adjusted (P<0.001), with a risk ratio of 0.62 within a 95% CI of [0.47; 0.81], favouring the use of nutraceuticals. The nutraceuticals used in the trials were green-lipped mussel, chondroitin sulphate, an extract of India and Java turmeric (P54FP), a homeopathic combination preparation known as Zeel®, Ω-3 fatty acids, deep sea fish oil, a mix of glucosamine with chondroitin sulphate and hyaluronic acid, and cannabidiol.

Basic therapy for viral diseases of the upper respiratory tract

The high prevalence of inflammatory diseases of the upper respiratory tract brings this pathology to the forefront, occupying up to 90% of cases of contacting specialists among all infectious diseases. The main etiological factor in the occurrence of acute inflammatory diseases of the upper respiratory tract, such as rhinitis, rhinosinusitis, pharyngitis, is the defeat of a viral agent. At the same time, the damaging viral agents are extremely diverse. In turn, an incorrectly selected initial therapy can lead to a weakening of local nonspecific immunity, which in turn forms a starting point for the attachment of a bacterial infection and the development of acute bacterial rhinosinusitis. Due to the high prevalence of infections of the upper respiratory tract and unreasonable systemic antibiotic therapy, it is the main factor contributing to the growth of antibacterial resistance, and taking into account the wide range of infectious microbiota that contributes to the development of chronic pathology, the selection of adequate drugs for the treatment of inflammatory diseases of the upper respiratory tract remains relevant. If it is inappropriate to use antibacterial therapy, homeopathic preparations can be used both as monotherapy for uncomplicated forms of a viral disease, and in combination in cases where the safety of prescribed drugs is important to the patient. This is especially true in childhood, when the doctor is limited in the use of medicines. The purpose of this work is to familiarize physicians with the epidemiology, symptoms and modern principles of treatment of inflammatory diseases of the upper respiratory tract with an emphasis on risk factors. The analysis of Russian-language and English-language publications was carried out using the databases: eLibrary, PubMed, Russian Science Citation Index, Springer, Scopus, Scientific Research, Crossref.

In vitro antimicrobial activity of nine homoeopathic preparations in different volumes against Staphylococcus aureus and Escherichia coli

Objective: The present study aims to evaluate the efficacy of antimicrobial activities of nine homoeopathic preparations through an in vitro study. Materials and Methods: The antimicrobial activity of nine homoeopathic preparations was evaluated through broth microdilution according to clinical and laboratory standards institute with slight modifications (M07-A10). Result: The mean and standard deviation of homoeopathic preparations against S. aureus and E. coli show lesser values than control and ethyl alcohol at lower volumes (10 μL and 15 μL, respectively). The broth microdilution assay provides the information that seven homoeopathic preparations exhibited significant results against S. aureus at 10 μL and eight homoeopathic preparations exhibited significant results against E. coli at 15 μL. Conclusion: The ultra-diluted homoeopathic preparations at different volumes especially lower volumes showed significant results against S. aureus (10 μL) and E. coli (15 μL) when compared to the negative control (91% ethyl alcohol). Further, in vivo studies may be carried out to confirm their mode and mechanism of action of homoeopathic preparations.

Homeopatic for treatment of cucumber seeds contaminated with auxinic herbicide

The aim of the present study was to investigate the use of homeopathic preparations to reduce the harmful effects caused by auxin herbicide residues on seed germination and early development of cucumber seedlings. The statistical design was completely randomized (DIC) in a 3x5+2 factorial scheme, with four replications. The treatments were the combination of three homeopathic preparations (Nux vomica, Carbo vegetabilis and Arsenicum album), in five centesimal dynamizations (6CH; 12CH; 18CH; 24CH and 30CH), plus two controls (T0= distilled water and TH = herbicide (Artys™) without homeopathic treatment). Each experimental unit consisted of transparent acrylic boxes (Gerbox™) with 25 cucumber seeds, distributed over two sheets of paper for germination and moistened with homeopathic treatments. The experimental units were kept in a BOD germination chamber (in a controlled environment at 25 ºC and 12 ho of light). On the tenth day after the installation of the experiment, the following variables were evaluated: percentage of germination, shoot length, root length, phytointoxication, percentage of dead seedlings and total dry mass. The homeopathic treatment of cucumber seeds contaminated with picloram + 2,4-D herbicide (Artys™) causes positive changes, causing interesting effects on seed germination, but it is not efficient for the total neutralization of the effect caused by the herbicide 2 picloram + 2,4-D and development of early cucumber seedlings. However, for some variables, the results are inconclusive with the preparations (Nux vomica, Carbo vegetabilis and Arsenicum album), and their ultradilutions (6CH, 12CH, 18CH, 24CH and 30CH), requiring more research to evaluate and describe the complexity of properties inherent to homeopathic treatment.

Therapeutic efficacy and safety of the use of the homeopathic preparation during teething in children

Introduction. The variability of the dentition terms is caused by numerous factors: the age and health of the parents, the parity, etc. Children may experience discomfort, which may be relieved by various pharmacological and non-pharmacological methods. Aim. To study the effect of factors on the temporary teeth eruption in children, the severity of discomfort symptoms and the therapeutic efficacy of a homeopathic medicine during the period of dentition.Materials and methods. The study included patients aged 4 months to 2 years. All children were divided into two groups. Group I included 97 apparently healthy children, Group II included 318 children with verified somatic pathology, who had residual effects of the perinatal hypoxic nervous system injury.Results and discussion. The analyses of correlation relationship between the dentition terms and medical and biological factors during pregnancy and childbirth in children showed the pronounced relationships: in group I – between the 53rd tooth eruption timing and the age of the mother, the mother’s disease during pregnancy, the threat of miscarriage (r = 0.98, p < 0.05); in group II – a high correlation coefficient between the 65th tooth eruption timing and acute infections suffered by the mother during pregnancy (r = 0.76; p < 0.05). Symptoms associated with teething were observed in 61% of children in group I and 72% (p < 0.05) in group II. Given the low awareness of parents about the homeopathic medicine (8.5%), we gave a talk and suggested using a homeopathic medicine during the next tooth eruption in a child.Conclusions. The temporary teeth eruption timetable is influenced by the course of the antenatal period, the mother’s age and the residual effects of the perinatal hypoxic nervous system injury. The homeopathic medicine is indicated to relieve symptoms of discomfort during the dentition period in the first year of life.

Cytotoxic Effects of Homeopathic Preparations on Human Tumor Cells In Vitro

Abstract: The aim of this work was to conduct an in vitro assessment of the possible cytotoxic effects of homeopathic drugs and consider possible mechanisms. Background: Homeopathy is still widely used as a complementary (alternative) medicine in different countries throughout the world. However, the method raises a lot of scientific debate about its effectiveness and mechanisms of action. This is especially true concerning the use of homeopathy in cancer treatment. Objective: This work aimed to comparatively assess the cytotoxic effect of homeopathic remedies on tumor cell cultures. Materials and Methods: We studied different dilutions of homeopathic medicines: Hydrastis, Conium and Carcinosinum. Cell lines of prostate cancer PC-3, breast cancer MDA-MB-231, and lymphoblastic leukemia Jurkat were used as model tumor objects. Standard colorimetric MTT test and cytometric analysis of cells were used here to assess the viability and parameters of apoptosis of cells. Results: Comparative assessment of the cytotoxic and apoptosis-inducing effects did not reveal significant differences in the viability of tumor cells in the groups exposed to homeopathic preparations among themselves and in comparison with the control group. The effects of the action of homeopathic remedies are shown only in the form of trends. Some possible mechanisms of the obtained results are considered. Conclusion: The results obtained in vitro indicated the absence of a direct cytotoxic effect in the studied homeopathic preparations. Despite the objective data, this does not allow us to make a final conclusion about the absence of antitumor activity of these drugs in vivo, since the possible or potential effects depend on the parameters of a living organism that are difficult to control and are often based only on hypothetical mechanisms. Some aspects of the action of homeopathy probably cannot be reproduced in vitro.

Self-assembled Patterns Formed in Evaporating Droplets to Analyze Bi-component Homeopathic Preparations in the Low Dilution Range.

Homeopathic complex remedies, composed of several homeopathic medicines in the low potency range, are frequently used in the treatment of a number of common disorders. At the same time, they represent an almost unexplored area of research. Are complex remedies just additive mixtures of the components, or are there interactions between the latter leading to new properties of the complex? In the present study, we analyzed as an example the simple bi-component complex, Luffa 4x - Mercurius bijodatus 9x, by means of patterns from evaporated droplets and tested what influences the complex's single compounds have upon the patterns and if there are any interactions. For this purpose, we compared in a series of five experiments patterns from evaporated droplets of the complex, Luffa 4x - Mercurius bijodatus 9x, and three comparison samples in which one or both of the complex's compounds were replaced by potentized solute. The patterns were photographed and evaluated for their gray-level distribution and texture using the software ImageJ. The experimental set-up's stability was tested by means of systematic control experiments. We found that Mercurius bijodatus 9x significantly influenced the patterns of Luffa 4x, increasing their homogeneity; at the same time, the patterns of Mercurius bijodatus 9x combined with solvent were more heterogeneous than those obtained from a control consisting of two pure solvents. In this phenomenological assay, the complex Luffa 4x - Mercurius bijodatus 9x does not correspond to a simple addition of the components. The exact nature of the underlying interaction needs to be elucidated in further investigations.

The use of a homeopathic preparation in the treatment of subclinical form of mastitis in cows.

Mastitis is a disease of productive cows, widespread throughout the world, and characterized by significant economic damage to the dairy industry. The subclinical form of this disease is aggravated by additional difficulties with its diagnosis and the lack of clear treatment protocols. Therefore, the study of the effectiveness of diagnostic studies and the search for new methods of treatment of latent forms of mastitis is an important direction in scientific research in countries with developed dairy cattle breeding. Studies conducted on the number of dairy cows of the production cooperative "Izhevsky" of the Akmola region of the Republic of Kazakhstan showed that when using rapid tests Kenotest, Somatest, Mastidine test, and Wideside test, the same results were obtained when the disease was detected in cows. The effectiveness of the tests was at the level of 60%-62% when using the settling sample as a control. Medical procedures were carried out using the Aquaton-2 microwave radiation apparatus and a homeopathic preparation. When using physiotherapy with microwave radiation, a decrease in the level of microbial contamination of milk from the treated part of the udder by 1.5-5 times was observed. Biologically active substances of plant origin in the homeopathic preparation, due to the immunostimulating effect, made it possible to increase the level of γ-globulins in the blood serum of sick animals during the application. The complex use of both methods in the treatment of animals with a subclinical form of mastitis made it possible to reduce the level of somatic cells in the milk of the affected udder lobe to a level that cannot be determined using Kenotest in 4-6 days, which is 2-4 days faster than using these methods separately.

Allergic Contact Stomatitis from Homeopathic Preparations (Arsenicum Album 30c) Used as Prophylaxis Against COVID 19

Contact stomatitis or stomatitis venenata is defined as an oral mucosa contact allergy caused by persistent contact with a pathogenic substance. Arsenic album 30 was advised as a “preventive and prophylactic remedy” against coronavirus disease-19 (COVID-19) by The Ministry of AYUSH (Ayurveda, Yoga and Naturopathy, Unani, Siddha, and Homeopathy), Government of India. We present a case of a 68-year-old man who suffered ulcers on the left buccal mucosa and tongue after taking arsenic album 30c for 10 days sublingually. This is, to the best of our knowledge, the first recorded incidence of allergic contact stomatitis caused by a homeopathic drug used to treat COVID-19. This case report emphasizes the need for obtaining a history, being aware of the composition of any new items, and reporting allergies or medicines provided to the patient.

Quantitative assessment of antioxidant potential of selected homeopathic preparations in clinical practice.

Antioxidant property like radical scavenging is a primary target to elucidate the efficacy mechanism of a drug against diseases linked to oxidative stress such as cancer, metabolic disorders, rheumatoid arthritis, etc. In alternative therapies, homeopathy is one of the preferred choices by patients and clinicians due to its potential to cure chronic and complex illnesses. However, the efficacy of homeopathic preparations at high diluted potencies attracts rational criticism due to insufficient scientific knowledge supporting the mechanism of action. Therefore, an attempt was made to estimate the total phenolic content (TPC) and radical scavenging activity of clinically prescribed homeopathic drugs. With gallic acid as a reference control, mother tinctures (MTs) and different potencies of Eucalyptus globulus (EG), Syzygium jambolanum (SJ), Ruta graveolens (RG), and Thuja occidentalis (TO) were used to perform Folin-Ciocalteu test, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The results showed TPC of MTs equivalent to µg/mL of gallic acid viz; EG (4,872.5±133.2), SJ (8,840.5±14.8), RG (985.6±39.1), and TO (341.5±19.5) with significant ABTS and DPPH radical scavenging potential. Whereas 30C and 200C potencies of each homeopathic drug showed undetectable phenolic content and insignificant radical scavenging potential compared to vehicle control, i.e.,alcohol 90% (2.0±1.5). The reported efficacy of 30C and 200C potencies of homeopathic medicines against oxidative stress-related illnesses might be due to mechanisms other than radical scavenging. Furthermore, the assays studied can be helpful in drug standardization and quality control of MTs that are used as starting material in homeopathic preparations.

Lycopodium clavatum as an inhibitor of monosodium urate crystallisation in gout: An in vitro study

Background: Hyperuricaemia plays a significant role in the development and pathogenesis of several metabolic and systemic disorders including metabolic syndrome, hypertension, stroke and atherosclerosis. Lycopodium clavatum is the most widely used drug in homoeopathy for treating hyperuricaemia and gout. However, its mechanism of action in reducing serum uric acid remains uncertain. Objective: The objective of the study was to study the potential role of homoeopathic preparation of Lycopodium clavatum in different potencies on monosodium urate crystallisation in vitro. Methods: Spectrophotometric crystallisation assay was carried out on a stock solution of 5 ml of uric acid after its preparation. The time course of the optical density was measured in a standard solution and values were measured every 5 min after agitation with cyclo-vortex mixer. The optical density values were also measured in the homoeopathic preparation of Lycopodium clavatum in different potencies with same method. Inhibiting effect of this medicine was found from graphs by measuring slope of nucleation and aggregation from optical density value and the percentage inhibition exerted by the proteins was calculated. Results: Spectrophotometric analysis showed all potencies of Lycopodium clavatum inhibited aggregation of monosodium urate crystals with a maximum inhibition at 200C and 1M potency. Conclusion: We found that the homoeopathic medicine Lycopodium clavatum could be effective in inhibiting the formation of monosodium urate crystals in vitro and also causes the dissolution of crystals, especially in high potencies. Further studies are required to understand the exact mechanism of action.

Syzygium jambolanum Homeopathic Formulation Improves Diabetes Modulating Adipogenic Genes in Diet-Induced Obese Mice: Comparison to the Standard Metformin Treatment

Background: Homeopathy is an alternative therapy that uses substances dilution. The homeopathic preparation of Syzygium jambolanum (SJ) has potential use in the treatment of glycemic disorders. Objective: In this context, the aim of the present study was to evaluate the use of the SJ homeopathic preparation on treating obesity and glycemic/metabolic disorders compared with metformin treatment (diabetes gold-treatment) in a mice model fed a high-fat diet. Method: A total of 48 male Swiss mice were divided into six groups with eight animals each, randomly distributed as follows: obese and non-obese groups (ST), treated and non treated with SJ or metformin. Obesity was induced by high-fat/high-sugar diet, while the control mice (ST) received a standard diet for 12 weeks, after which, for 30 days, the animals received a preparation of 20 µl/100g/body-weight of SJ or a dose of 250 mg/kg metformin (by gavage). The control groups ST and HFHS received water gavage. Body composition and biochemical parameters were evaluated. The adipose genes for lipogenesis and thermogenesis were tested. Results: The mice treated with Syzygium jambolanum presented reduced weight gain (p< 0.02) and smaller adipose tissue weight, average difference from the mice treated with metformin, and both groups showed improved insulin sensitivity (p<0.04). The main results were associated with the modulation of the adipogenesis pathway (C/EPBα and PPARγ) and to the thermogenic gene Uncoupling Protein 1 (UCP1). Conclusion: Homeopathic administration of the Syzygium jambolanum preparation leads to lower body weight, blood glucose and total cholesterol and higher high-density lipoprotein (HDL), pointing its efficiency on treating diabetes and obesity.

The effectiveness of using a homeopathic preparation containing calendula, witch hazel, aesculus, menthol and zinc oxide in patients with nasal obstruction in vasomotor and chronic subatrophic rhinitis

Introduction. In recent years, the world has seen an increase in the number of patients with difficulty in nasal breathing due to nasal obstruction, one of the causes of which are functional disorders of the nasal mucosa in various forms of rhinitis. Frequent use of intranasal decongestants among the population further contributes to the lack of effectiveness of conservative treatment and the need for surgical correction to restore free nasal breathing. This article discusses the possibility of using a homeopathic preparation containing calendula, witch hazel, aesculus, menthol and zinc oxide in patients with nasal obstruction in vasomotor and chronic subatrophic rhinitis to stop the clinical manifestations of the disease and improve the quality of life of patients. Аim. Тo evaluate the effectiveness of a drug containing calendula, witch hazel, aesculus, menthol and zinc oxide in patients with vasomotor and chronic subatrophic rhinitis in the complex treatment of nasal obstruction. Мaterials and methods. The study involved 48 adult patients of both sexes, aged 20 to 50 years, diagnosed with vasomotor (idiopathic) rhinitis in 24 and chronic subatrophic rhinitis in 24 patients. The studied preparation containing calendula, witch hazel, esculus, menthol and zinc oxide was smeared on the mucous membrane of the nasal cavity by laying gauze turundas for 5 minutes, 3 times a day, while the course of treatment was 10 days. The efficacy and safety of the drug was assessed by the dynamics of the severity of subjective symptoms, objective clinical data during control examinations by an otorhinolaryngologist in points, as well as by the presence of side effects in the treatment of the disease. Results. Results of the study showed a pronounced positive trend in the form of relief of subjective complaints and the main clinical manifestations of the disease, accompanied by an improvement in the quality of life in patients in both groups examined during treatment. Side effects were not observed in patients in both study groups. Conclusions. Based on the results of the study, conclusions were drawn about the efficacy and safety of using a complex homeopathic preparation containing calendula, witch hazel, esculus, menthol and zinc oxide in an adult group of patients with nasal obstruction, which makes it possible to recommend it for widespread use for the complex treatment of nasal syndrome. obstruction in vasomotor and chronic subatrophic rhinitis, as well as other forms of rhinitis accompanied by nasal obstruction syndrome.

A study to evaluate the effect of gymnema sylvestre in mother tincture and 50 millisimal doses on diabetes mellitus - type-2

Background: Diabetes Mellitus is metabolic disorder characterized by hyperglycemia resulting from inadequate insulin secretion, insulin inaction or both and insulin resistance. People living with Type-2 DM are more vulnerable to various forms of both short-term and long-term complications, which often lead to their premature death. Gymnema sylvestre, in various homoeopathic preparations, have reported beneficial effect for treating in Type-2 diabetes mellitus. Aim and Objective: To understand the efficacy of Gymnema sylvestre in 50milisimal potency and in mother tincture on diabetes mellitus Type-2 patients. Method: This prospective, open label, randomized controlled trial was conducted at OPD and IPD of Government Homoeopathic Medical College and Hospital, Bhopal and camps organized by department. A total 60 nos. of patients were enrolled for this study considering all ethical criteria and inclusion and exclusion criteria’s as per institutional guidelines with 30 cases allotted randomly to GSMT and LM group. Data were recorded on standard case proforma, and entered Microsoft® Excel worksheet 2019, and exported into SPSS v21. 0 (IBM, USA) for statistical analysis. Each case was followed up for a period of six months and outcome were assessed on QOLID scale. Normality of data was determined using Shapiro-Wilk test. Normally distributed variables were compared between two groups using independent t-test. Comparison within a group at two time-intervals was made using paired t-test. P value

Evaluation of hypolipidemic activity of homeopathic drug Allium sativum 6C potency on different grades of dyslipidemia in Wistar albino rat models

BackgroundUnhealthy dietary patterns and lifestyle modification could exacerbate heart-related diseases, and the risk factors for cardivascular disease mortality includes dyslipidemia, hypertension, and diabetes mellitus. However, controlling cholesterol, triglycerides, and low-density lipoprotein could minimize the occurrence of risk factors. Although homeopathic preparation of Allium sativum is effective in treating various ailments, it's therapeutic usage by the homeopathic practitioners must be validated scientifically to ascertain its action. ObjectivesThe present study is designed to evaluate the hypolipidemic and anti-inflammatory activities of the homeopathic drug A. sativum 6C in mitigating dyslipidemic changes in different types of dyslipidemic rat models. MethodsAssessment of serum biochemical parameters, evaluation of liver cytokines, and immunoblot analysis of inflammatory proteins were performed in this study. ResultsAdministration of a homeopathic preparation of A. sativum 6C to different types of dyslipidemic rat model showed a significant improvement in the serum lipid profile, reduction in the activity of serum marker enzymes (AST, ALT, LDH, and ALP), and decrement in the levels of liver inflammatory cytokines (TNFα, IL-6, and IL-1β). Further, the expression of inflammatory proteins (CRP, P-Selectin, E- Selectin) down regulated in the liver of dyslipidemic animals upon administration of A. sativum 6C. However, among the various dyslipidemic models, the efficacy of A. sativum is more profound in the HCD and HFD-fed animals. Analyzing the GC–MS data of A. sativum 6C revealed that it contains some active ingredients (Undecane; 1-methylene 1H-Indene, and Oxazolam) that might be responsible for the anti-inflammatory properties of A. sativum 6C. ConclusionHomeopathic preparation of A. sativum 6C modulates the levels of serum biochemical parameters and inflammatory events in HCD-T and T2DM-T animals than in the STZ-T animals, mitigating metabolic dysfunction. Therefore, this study brings limelight to support the potency of the homeopathic drug A. sativum for therapeutic purposes in correcting metabolic dysfunction associated with the current lifestyle.

Cholesterol lowering activity of homoeopathic preparations of Colchicum autumnale – An in vitro study

Background: Total cholesterol content of erythrocyte membranes plays a critical role in atherosclerotic plaque progression and instability. Several homoeopathic medicines are known to have a significant role in the control of hyperlipidaemia. Colchicum autumnale is a plant remedy used mainly for gout, but also for hyperlipidaemia. Objectives: This is a study to assess the in vitro anti-cholesterol action of the Colchicum autumnale (Colch. at.) on the erythrocyte membrane. Materials and Methods: The study was conducted using a hyperlipidaemic blood sample which was divided into eight parts. Six different potencies of Colch. at. were tested, and two controls, one negative and another positive were used. Cholesterol was estimated using Liberman Burchard reagent and spectrophotometry. Results: In a hyperlipidemic blood sample, the range of reduction of cholesterol concentration was from 18.20% to 19.16%. The 6C potency removed 18.24% of cholesterol,12C potency removed 18.50% cholesterol,30C potency removed 19.02% cholesterol,200C potency removed 19.68% cholesterol and 10M potency removed 19.16% cholesterol. The maximum effect was seen in 1M potency where the reduction was 19.68 %. Conclusion: This study suggests that the Colchicum autumnale might be useful for the management of hypercholesterolemia.

Silicea terra 200cH evaluated by two different spectroscopy methods: a pilot study

Abstract
 Spectroscopy has been shown to be a useful method to study the physicochemical properties of homeopathic preparations. In this pilot study, the aim was to compare two methods (photon scattering and visible-light spectroscopy) in the physical evaluation of Silicea terra 200cH. Two samples have been examined and compared: Silicea terra 200cH and Sac lac 200cH, both prepared in aqueous solution (Cemon Lab, Italy). Lactose was included because the first 3 potencies of Silicea terra are made by trituration in lactose. In the first test, performed in Italy, slight variations in chrominance and luminance due to micro-vibrational 3D phenomena were analyzed. The principle of the experimental procedure is to modify and analyse the incidence and refraction angles of the light hitting the bottles of liquid under examination. Measurements were made using an innovative camera device developed by Daniele Gullà, called MIRA/CORA (proprietary name). The image sensor is a Charge-Coupled Device (CCD)/ Complementary Metal Oxide Semiconductor (CMOS) with interferential matrix filter. A red laser beam, at wavelength 652 nm, hits the bottle and the variations of scattered light are recorded and measured by the device. All measurements have been performed in a dark room at a constant temperature of 22°C +/- 0,2°C using a filter with very narrow spectral bands (10 nm). Proprietary software analyses the incoming frames showing the spectral variations in amplitude and frequency and then interpolates variation in the measured data within a vibrational range between 0.01 Hz and 10 Hz. The measurements have been repeated three times on both Silicea terra 200cH and Sac lac 200cH within a few minutes of each other. Measurements of the mean entropy of the signals, statistically elaborated with T Student test, yielded a two tailed p value < 0.05, where the entropy of the signal recorded from the Silicea terra 200cH sample was statistically lower than the 200cH Sac lac sample. In the second test, performed in Brazil, variations in absorbance were used to identify Silicea terra 200cH compared with Sac lac 200cH and a control solution of non-succussed 30% alcohol, using six solvatochromic dyes, following the method developed by Cartwright [1,2]. Both homeopathic samples were imported from Italy, diluted 1:100 in 30% hydro-alcoholic solution, and submitted to 100 succussions using an automatic mechanical arm (Denise, AUTIC, Brazil) prior to being tested. Samples were inserted into dyes solutions in a 1:60 ratio, and evaluated by visible spectroscopy (FEMTO Spectrophotometer, Brazil). Dyes were prepared in ethanol P.A., according to previous established methods [3]. Samples and dyes were protected from light during the manipulation and environmental conditions of the laboratory (temperature, humidity, and magnetic flux) were monitored during the tests. Three series in triplicate were performed and the results were analyzed by ANOVA / Tukey, comparing both samples and the unsuccussed 30% hydroalcoholic control solution. Among all tested dyes, only BDN showed an interaction with Silicea terra 200cH, reproducing the conclusions obtained in [3]. We conclude that two different spectroscopic methods were able to differentiate Silicea terra 200cH from Sac lac 200cH, suggesting that changes in solvent organization could be involved in the homeopathic signaling process, along with changes in dipole moments of solvent and dyes. The results are potentially in line with a recent published paper [4], that supports the proposition that the lower entropy of the verum signal compared with controls could be explained by increased coherent vibrations of the verum sample, modulated by Nambu-Goldstone bosons. According to Del Giudice and colleagues [5], coherent behaviors are correlated with massless quasi-particles, coding ordering information.