Homemade Device Research Articles

Photothermal triggered clinical swab point-of-care testing diagnostics: fluorescence-pressure multi-signal readout detection of cervical cancer biomarker

In this protocol, a photothermal triggered multi-signal readout point-of-care testing (POCT) was established in a multifunctional clinical vagina swab for Human papillomavirus type 16 E6 (E6 protein) determination. Particularly, a portable vagina swab was employed as a sensing substrate integrated collection and analysis of samples. The composite probe SiC-CS@Ag with the chitosan (CS) decorated silicon carbide nanoparticles (SiC NPs) as a label was bounded to the swab via competitive reaction. To transfer recognition events into detectable signals, a flexible fluorescence -temperature indicator (FLTI) with thermal-responsive fluorescence capability was assembled on swab surface followed by sealing it into a home-made tiny pressure device and H2O2 as a signal reporter was introduced. Under near-infrared (NIR) laser expose, remarkable photothermal effect from SiC-CS@Ag caused notable temperature elevation, which lead to significant fluorescence (FL) quenching on FLTI. Meanwhile, increased temperature also enhance the catalase-like activity of SiC-CS@Ag toward H2O2 decomposition that cause significant pressure elevation. This bioassay realized sensitive target detection in the linear ranger from 10-6 ng/mL to 1 ng/mL with detection limits of 1.60 × 10-6 ng/mL. Such a photothermal mediated multi-signal readout sensing platform not only provides rapid and precise methodologies for biomarker detection, but also enriches strategies for POCT and boosted its application for early diagnosis of malignant diseases in remote areas.

Impact of hydro-chemical conditions on structural and hydro-mechanical properties of chalk samples during dissolution experiments

The importance of karst reservoirs for water resources, and their complexity in terms of structural properties and hydraulic responses, require a better understanding of the formation and location of conduits in these systems, in particular for chalk reservoirs. For this purpose, we conduct laboratory experiments of acid solutions injection in chalk core samples using a homemade experimental device. Three different flow rates and two acid concentrations are applied on six samples at atmospheric conditions with Peclet numbers ranging from 0.1 to 1.7 and Damköhler numbers from 10−6 to 10−3. Hydraulic and chemical measurements are carried out during the experiments, while petrophysical characterization is conducted on the samples before and after the reactive percolation experiments. The analysis of these data shows an increase in permeability of the rock samples due to calcite dissolution, which is associated with the creation of preferential conduits. We also observe that the initial structural properties control the dissolution regime with the following main conclusions. For homogeneous systems, the overall dissolution rate and percolation time are directly linked to the considered flow rate and injected acid concentration. For heterogeneous systems, (i) pre-existing large-scale structural heterogeneities control the location of newly created paths while reducing the value of the dissolution rate, and (ii) micro-scale heterogeneities result in highly heterogeneous general structures, which are responsible for channels formation while applying low Damköhler numbers.

Handheld Platform for Sensitive Rosiglitazone Detection: Immunosensor Based on a Time-Based Readout Device.

The detection of rosiglitazone (RSG) in food is of great importance since the excessive intake of RSG could cause adverse effects on the human body. Although liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry are the preliminary methods for the detection of hazardous materials in food, they are not suitable for point-of-care or on-site detection. Herein, a time-based readout (TBR) device with an application software (APP) controlled by a smart phone was developed for the sensitive and selective immunoassay of RSG. The homemade TBR device was based on a two-electrode system, where the immune molecule-modified glassy carbon electrode was used as the bioanode, and Prussian blue-modified FTO was used as the cathode. By using Au-modified octahedral Cu2O with high catalytic activity as mimetic peroxidase, an insulating layer was generated on the cathode by catalyzing 4-chloro-1-naphthol (4-CN) into benzo-4-chlorohexadienone (B4Q). The time to reach a fixed potential varied indirectly with the concentrations of RSG and was recognized by the APP, while the electrochromic property on the cathode was also correspondingly changed. Under optimum conditions, both the square root of the time and the chroma value of the electrochromism exhibited linear responses for the detection of RSG ranging from 5 × 10-10 to 5 × 10-7 g/L, while the limits of detection were 8.2 × 10-11 and 1.3 × 10-10 g/L, respectively. With easy operation and portability, this TBR device showed a promising application for point-of-care monitoring of hazardous materials in food or the environment.

A homemade device for simultaneous measurement of pulmonary ventilation and metabolic rate in neonatal rodents

Various in vitro neonatal rodent models have been developed to study the control of breathing, but translation of the information requires a behavioral assay, which has led to the widespread use of plethysmography to measure breathing in awake neonatal rodents. Best practice requires correcting changes in ventilation to the corresponding change in metabolic rate, which is the main driver of pulmonary ventilation. Obtaining measures of both simultaneously is ideal, though technically difficult. Here we describe a simple, inexpensive home-made dual chamber approach for simultaneous measurement of pulmonary ventilation and metabolic rate. We found that the dual chamber provides values for pulmonary ventilation and metabolic rate that compare favorably with existing approaches.

Electrochemiluminescent “turn-on” chemosensor based on the selective recognition binding kinetics with glutathione

Recently, there have been urgent demands for decentralized, point-of-care (POC) diagnostics for digital healthcare, telemedicine, and individual self-assessment. A chemosensor producing electrochemiluminescence (ECL) upon molecular recognition is distinctly advantageous as it offers sensitive target signals without the need for bulky instrumentation and highly trained personnel. Herein, we report an Ir(III)-based chemosensor (1) that selectively generates ECL signal only at the content of glutathione (GSH) among various interferences. Our sensing strategy is based on the binding stability between the 1 and GSH. 1 possesses the terpyridine-Cu(II) branch as a recognition unit undergoing complexation reaction by various biothiols in human serum. The binding motif of 1 is structurally less selective and weak to be easily broken by dissolved oxygen. However, 1 exhibits long-lasting stability only with GSH enabling quantitative chemosensing for GSH. Time-resolved fluorescent assay along with the NMR and mass spectrometry study reveals that GSH permits the stable complexation with 1. The ECL approach additionally provides high detection sensitivity and selectivity towards GSH, which is challenging in fluorescence assay. Various coreactant ECLs, including oxalate (C2O42-), tri-n-propylamine (TPA), and peroxydisulfate (S2O82-), were investigated to find optimal conditions for the ECL chemosensing. After 15 min incubation in a 40 μL deproteinized serum sample, 1 successfully reports the GSH content by yielding an efficient ECL signal in a compact, homemade ECL device. The ECL “turn-on” signal provides a linear correlation only to the GSH level, which is 0–25 μM under a detection limit of 1.47 μM.

Ice breaking by a high-speed water jet impact

Ice breaking has become one of the main problems faced by ships and other equipment operating in an ice-covered water region. New methods are always being pursued and studied to improve ice-breaking capabilities and efficiencies. Based on the strong damage capability, a high-speed water jet impact is proposed to be used to break an ice plate in contact with water. A series of experiments of water jet impacting ice were performed in a transparent water tank, where the water jets at tens of metres per second were generated by a home-made device and circular ice plates of various thicknesses and scales were produced in a cold room. The entire evolution of the water jet and ice was recorded by two high-speed cameras from the top and front views simultaneously. The focus was the responses of the ice plate, such as crack development and breakup, under the high-speed water jet loads, which involved compressible pressure ${P_1}$ and incompressible pressure ${P_2}$ . According to the main cause and crack development sequence, it was found that the damage of the ice could be roughly divided into five patterns. On this basis, the effects of water jet strength, ice thickness, ice plate size and boundary conditions were also investigated. Experiments validated the ice-breaking capability of the high-speed water jet, which could be a new auxiliary ice-breaking method in the future.

'All In One' SARS-CoV-2 variant recognition platform: Machine learning-enabled point of care diagnostics

Point of care (PoC) devices are highly demanding to control current pandemic, originated from severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). Though nucleic acid-based methods such as RT-PCR are widely available, they require sample preparation and long processing time. PoC diagnostic devices provide relatively faster and stable results. However they require further investigation to provide high accuracy and be adaptable for the new variants. In this study, laser-scribed graphene (LSG) sensors are coupled with gold nanoparticles (AuNPs) as stable promising biosensing platforms. Angiotensin Converting Enzyme 2 (ACE2), an enzymatic receptor, is chosen to be the biorecognition unit due to its high binding affinity towards spike proteins as a key-lock model. The sensor was integrated to a homemade and portable potentistat device, wirelessly connected to a smartphone having a customized application for easy operation. LODs of 5.14 and 2.09 ng/mL was achieved for S1 and S2 protein in the linear range of 1.0–200 ng/mL, respectively. Clinical study has been conducted with nasopharyngeal swabs from 63 patients having alpha (B.1.1.7), beta (B.1.351), delta (B.1.617.2) variants, patients without mutation and negative patients. A machine learning model was developed with accuracy of 99.37% for the identification of the SARS-Cov-2 variants under 1 min. With the increasing need for rapid and improved disease diagnosis and monitoring, the PoC platform proved its potential for real time monitoring by providing accurate and fast variant identification without any expertise and pre sample preparation, which is exactly what societies need in this time of pandemic.

Study on the Tribological Properties of F-T DS/ZnFe-LDH Composite Lubricating Material

The homemade soot capture device was used to burn Fischer-Tropsch synthetic diesel (F-T diesel) in order to simulate the combustion of F-T diesel in the engine and collect its soot (F-T DS, FS). The zinc-iron hydrotalcite (ZnFe-LDH) and the composite materials of FS and ZnFe-LDH (F-T DS/ZnFe-LDH, FS/ZnFe-LDH) were prepared by hydrothermal synthesis, and the similarities and differences in tribological characteristics of the above three lubricating materials such as 10# white oil (10# WO) lubricant additives were investigated. FS is an aggregation composed of amorphous carbon and graphite microcrystals. ZnFe-LDH is mainly composed of nanosheets, Zn, and Fe hydroxide particles, with a high degree of crystallization, while FS/ZnFe-LDH is a “sandwich layer” composed of nanosheets and soot particles. Because of the addition of cetyltrimethylammonium bromide and the grafting of a long carbon chain lipophilic group in the preparation process, FS/ZnFe-LDH has better anti-wear properties than the FS and ZnFe-LDH Effect. When FS/ZnFe-LDH is added at 0.2 wt.%, the average friction coefficient (AFC) and average wears scar diameter (AWSD) are at their lowest. Compared with pure 10# WO, the minimum values of AFC and AWSD have dropped by 36.84% and 22.58%, respectively. XPS analysis of the wear scar surface shows that when ZnFe-LDH and FS/ZnFe-LDH are used as lubricating additives of 10# WO, together with the organic matter in the white oil and the iron element in the friction pair, tribochemistry occurs under the combined action of the adsorption force and the tribochemical reaction, a friction protection film containing four elements of C, O, Fe, and Zn is formed on the surface of the wear scar, which effectively reduces the wear and reduces the friction coefficient.

Preparation of CsPbBr<sub>3</sub> perovskite nanocrystals with controllable morphology and <i>in-situ</i> photoluminescence of formation kinetics

Cesium-lead halide perovskite nanocrystals (CsPb<i>X</i><sub>3</sub> (<i>X</i> = Br, Cl, I) PNCs) have become ideal luminescent materials for wide color gamut display devices, white LED lighting and high-efficiency solar cells, due to adjustable energy band gap, high fluorescence quantum yield, narrow fluorescence emission peak, and ultra-high defect tolerance. The preparation of CsPb<i>X</i><sub>3</sub> PNCs with controllable size and morphology is a prerequisite for obtaining efficient and stable photovoltaic/photovoltaic devices. In this report, the CsPbBr<sub>3</sub> PNCs with different shapes are prepared by adding different concentrations of dodecanedioic acid (DDDA) ligands at room temperature through using ligand-assisted reprecipitation method. Utilizing the X-ray diffractometer, transmission electron microscopy, ultraviolet spectrophotometer, fluorescence spectrometers (PL), the phase structure, microstructure and optical properties of the nanocrystals are investigated. The results show that the presence of DDDA ligands have no influence on the phase structure of nanocrystal products, they all present a cubic phase structure. Surprisingly, the morphology of the nanocrystals gradually transforms from nanocubes into nanoplatelets with ～5 layers in thickness as the concentration of DDDA increases. In addition, the PL spectrum shows a significant blue shift from 509 nm to 478 nm. By using the <i>in-situ</i> homemade PL device with ultra-high time resolution (～100 ms), the real-time monitoring PL spectra of nanocrystals in the formation process are measured. The results demonstrate that nanocrystals undergo rapid nucleation and focusing of size distribution growth to generate nanocubes in the absence of DDDA ligand. When the DDDA ligand is present, nanocrystals are mainly nanoplatelets in the early growth stage due to the decelerated reaction. As the reaction proceeds, nanocubes can emerge and grow gradually while the nanoplatelets disappear when the concentrations of DDDA ligands are 25% and 50%. As the concentration is further increased to 75%, almost nanoplatelets could be formed after the nucleation stage and growth stage. Unexpectedly, preformed nanoplatelets are unstable for the prolonged reaction time as a result of the high surface energy, and they will eventually transform into isotropic nanocubes through dissolution-recrystallization pathway, indicating that the process in the later stage is controlled mainly by thermodynamics. Our findings offer an efficient strategy to synthesize the perovskite nanocrystals with controllable size and morphology.

Tuning the optical absorption performance of MoS2 monolayers with compressive strain.

Strain engineering has been extensively applied as a promising strategy in the regulation of physical and chemical properties of two-dimensional (2D) materials, which remarkably broadens their application prospects in flexible electronics and chip manufacturing. However, the difficulty in fixing a flexible substrate under compression and the challenge in adjusting the focal distance have hindered the in-depth investigation of compressive strain. Here, we fabricated a home-made strain loading device and proposed a compressive strain measurement method, via which the strain-dependent optical absorption properties of MoS2 monolayers under compression has been studied. According to the measured optical absorption spectra, the first blueshift and then redshift trend under compression was obviously observed. The reliability of the experimentally observed trend in peak position shift was theoretically verified by density functional theory calculation. Our work offers a feasible way to characterize optical properties of 2D materials under compressive strain and expands the space for the development of next-generation micro/nano-scale optoelectronic devices.

Design and Construction of Home-Made Spin Coater for OLED Production

This study devotes to the design and fabrication of a spin coating equipment using locally available materials for the deposition of uniform thin films on several substrates and also the production of organic light emitting diodes (OLEDs) using this home-made device. The equipment consists of a brushed direct current (BDC) motor with high speed, chuck (substrate

Nonaqueous capillary electrophoresis and quantum chemical calculations applied to investigation of acid-base and electromigration properties of azahelicenes.

Nonaqueous capillary electrophoresis (NACE) using methanol (MeOH) as a solvent of the BGEs and quantum mechanical density functional theory (DFT) have been applied to determine the thermodynamic acidity (ionization) constants (pKa ) of mono- and diaza[5]helicenes, mono- and diaza[6]helicenes, and their dibenzo derivatives in MeOH and water. First, the mixed acidity constants, , of ionogenic pyridinium groups of azahelicenes and their derivatives in MeOH were obtained by nonlinear regression analysis of pH dependence of their effective electrophoretic mobilities. The effective mobilities were measured by NACE in a large series of methanolic BGEs within a wide conventional pH range (pHMeOH 1.6-12.0) and at ambient temperature (21-26°C) in a home-made CE device. Prior to mixed acidity constant calculation, the effective mobilities were corrected to reference temperature (25°C) and constant ionic strength (25mM). Then, the mixed acidity constants were recalculated to the thermodynamic acidity constants pKa,MeOH by the Debye-Hückel theory of nonideality of electrolyte solutions. Finally, from the methanolic thermodynamic pKa,MeOH values, the aqueous thermodynamic constants were estimated using the empirical relations between methanolic and aqueous acidity constants derived for structurally related pyridine derivatives. Depending on the number and position of the nitrogen atoms in their molecules, the analyzed azahelicenes were found to be weak to moderate bases with methanolic pKa,MeOH in the range 2.01-8.75 and with aqueous in the range 1.67-8.28. The thermodynamic pKa,MeOH obtained by the DFT calculations were in a good agreement with those determined experimentally by NACE.

Interfacial polarization in ultra-small Co3S4−MoS2 heterostructure for efficient electrocatalytic hydrogen evolution reaction

Constructing suitable heterostructured electrocatalysts and understanding their interfacial effects have been considered as an effective strategy to improve the intrinsic activity of electrocatalytic hydrogen evolution reaction (HER). In this work, ultra-small Co3S4-MoS2 heterostructure was synthesized using a precursor solubility controlling method. It is found that the electrocatalytic HER performance of the heterostructured catalysts exhibited a low overpotential of 260 mV at 100 mA cm−2 in 1.0 M KOH and possessed remarkable stability over 100 h continuous electrolysis at high current density (100 mA cm−2). Besides, Co3S4-MoS2 electrocatalyst is applied as the cathode material for a homemade membrane electrode device for water electrolysis to produce hydrogen. It shows that the electrocatalyst has a comparable catalytic activity to that of 20% Pt/C catalyst at 70 °C. The outstanding performance of the ultra-small Co3S4-MoS2 heterostructure is attributed to the efficient mass transport benefitting from its unique ultra-small hollow structure and the rapid electron transfer at the interface between Co3S4 and MoS2. XPS characterizations and DFT calculations confirm that the interfacial polarization and the corresponding electron transfer from Co3S4 to MoS2 at the interface optimizes the charge state of the catalyst and tunes the binding strength to the intermediates during each elementary step of the HER process. To be exact, the electron transfer promotes the adsorption of OH* on the charge-depleted Co3S4 sites and the adsorption of H* on the charge-enriched MoS2 sites, respectively, thereby reducing the free energy barrier of the water dissociation step (rate-limiting step) and improving the reaction kinetics. The solubility controlling strategy may provide a novel idea for rational design and synthesis of superior stability transition metal sulfides HER electrocatalysts.

Integrated sample‐pretreatment strategy for separation and enrichment of microplastics and primary aromatic amines in the migration of teabag

In this work, an integrated sample-pretreatment strategy for the separation and enrichment of microplastics and primary aromatic amines from the migration of teabag was developed. The migration solution of teabag was passed through a homemade device. The microplastics were firstly captured by a silver membrane, and then the primary aromatic amines were enriched by a solid-phase extraction column. The microplastics migrated from teabag were detected by attenuated total reflection-Fourier transform infrared spectrometer and Raman spectroscopy. The data showed the character, the number of particles, area ratio, and morphology of microplastics migrated from the teabag. Subsequently, after the enrichment procedure, a sensitive analytical method for primary aromatic amines was established followed by ultra-high performance liquid chromatography-tandem mass spectrometry. The method showed wide linear ranges with R2 greater than 0.9915, low limits of detection (2-18ng/L), and low limits of quantification (8-50ng/L). The developed method was adopted to analyze microplastics and primary aromatic amines migrated from nylon and polyethylene terephthalate teabag under different temperatures and times. The integrated sample-pretreatment strategy displayed promising potentials in the one-step preparation of the microplastics and hazardous molecules in the sample of environment and food security.

High-sensitivity micro-strain sensing using a broadband wavelength-tunable thulium-doped all-fiber structured mode-locked laser.

We demonstrate a thulium-doped mode-locked fiber laser with ultra-broadband wavelength tunability for micro-strain sensing based on the multimode interference (MMI) effect in single-mode-multimode-single-mode (SMS) fiber configuration. The homemade SMS device with high performance is fusion spliced in the laser cavity, and the developed dispersion precisely managed the all-fiber structured mode-locked picosecond laser with a record-breaking wavelength tuning range from 1976 to 1916 nm while exerting axial strain on this SMS device. We experimentally explored the regularity between the strain and the central-wavelength shift of the mode-locked pulse, and for the first time to the best of the authors' knowledge, achieved the precise in-line axial strain measurement from 0 to 5385 µɛ by using the tunable ultrafast-laser-based sensor, and sensitivity is up to -11.5 pm/µɛ. With high compactness and durability, this sensor has advantages in real-time dynamic measurement over other passive devices, thus will undoubtedly find various application scenarios.

Impact of particle stiffness on shear-thinning of non-Brownian suspensions

We attempt to identify the impact of particle stiffness on the shear-thinning behavior exhibited by frictional and adhesive non-Brownian suspensions. To this aim, we compare the rheological behavior of three suspensions whose particles have different Young’s moduli. The stiffest particles are soda-lime glass beads with a Young modulus of 70 GPa. The two other kinds of particles are made of polydimethylsiloxane and have been manufactured using a homemade millifluidic device that allowed us to produce batches of sparsely polydisperse particles with tunable Young modulus: 1.8 MPa for the softest ones and 15 MPa for the stiffest. We show that the observed shear-thinning is mainly caused by the presence of adhesive forces between particles. For each of the three suspensions, the shear-thinning behavior is described by the variation of the jamming volume fraction, ϕm, with stress, σ, and the results are interpreted in the light of the model proposed by [Richards et al., J. Rheol. 64(2), 405–412 (2020)]. We show that the magnitude of variation of ϕm with σ is greater the lower the particle stiffness. More precisely, in the adhesive regime, decreasing the particle stiffness leads to a significant decrease in the value of the jamming fraction while, as predicted by the JKR theory, the characteristic adhesion stress, σa, does not vary with stiffness. We, finally, show that, for stresses much higher than σa, the suspensions behave like usual frictional suspensions with a viscosity controlled by both the particle volume fraction and the value of the interparticle friction coefficient.

High-efficiency self-charging power systems based on performance-enhanced hybrid nanogenerators and asymmetric supercapacitors for outdoor search and rescue

Self-charging power systems (SCPSs) that combine energy harvest/store technologies have attracted extensive attention owing to their vast potential in the Internet of things (IoT). Still, the long charging time is a severe obstacle to the practical use of SCPSs. Herein, we have successfully prepared a high-efficiency SCPS composed of a performance-enhanced rotational hybrid nanogenerator (R-HNG) and high-energy-density asymmetric supercapacitor (ASC). The R-HNG consists of a rotational triboelectric nanogenerator (R-TENG) and an electromagnetic generator (EMG), exhibiting superior charging performance. Among them, the output characteristics of the R-TENG can be enhanced by adjusting the work function difference between the friction layer and electrode. The working principle of the R-HNG is systematically explained by both Kelvin probe force microscope (KPFM) and finite element simulation. Meanwhile, the as-prepared ASC based on honeycomb-like NiCo-layered double hydroxides (LDH) nanosheets are used as an energy storing device within SCPS. The SCPS can reach 5.9 V after 120 s under a rotation speed of 200 rpm, which can power a homemade positioning device for outdoor search and rescue. This work provides a novel and effective solution for constructing high-performance SCPSs and is expected to accelerate the development of SCPSs in the IoT.

Head, chest and limbs mine-explosive wounds

The article presents the experience of treating wounded K., 29 years old, who, as a result of the suicide bombing of a homemade shell-free explosive device in a moving car of the St. Petersburg Metro 03.04.2017 received a severe mine-explosive combined wound to the head, chest and limbs with a fragmented blind skull. The trauma was accompanied by brain damage, a fracture of the cranial vault bones, lungs contusion, and fragmented fracture of the right tibia bones. The patient underwent sequential bifrontal decompressive craniotomy, external fixation of the right shin bones fractures, blocked intramedullary osteosynthesis of the latter, dura mater plasty, cranioplasty with a titanium plate after its computer modeling. The complex treatment allowed the patient to be discharged from the hospital after 4 months in a satisfactory condition.

Study on development of a device for assessment compost stability based on the determination of oxygen consumption using pressure measurement

Organic fertilizer or compost is an essential product in the current trend of high-tech agricultural development. Compost stability is not only an important quality parameter but can also be used to monitor the efficiency of the composting process. This study developed a device to evaluate the stability of compost based on the oxygen consumption method using the principle of pressure measurement. This homemade device has improved design, overcoming existing weaknesses in commercial equipment with the same operating principle. In which, the compost sample is put in the containers placed in the middle of the bottle while the produced CO2 is absorbed by the KOH solution at the bottom. The device is capable of working independently with the data recorded on the microSD card without connecting to a computer. The device is operationally tested in the laboratory to assess the oxygen consumption of two actual compost samples. The results showed that these samples both meet the EU’s regulations on compost stability with oxygen consumption in 4 days (AT4) less than 10.0 mg O2/g compost.

Suprapubic Transvesical Repair of Vesicovaginal Fistula Using a Homemade Laparoscopic Single-Port Device: Experience of 42 Patients.

Aim: Vesicovaginal fistula (VVF) is the most common urogenital acquired fistula, and has remained a scourge and of public health importance. VVF can be repaired by transvaginal approach, transabdominal approach or transvesical approach, but the optimal management is still debated.Methods: To demonstrate a suprapubic transvesical approach to repair VVFs using a homemade laparoscopic single-port device. A retrospective review of the medical records of 42 consecutive patients who underwent fistula repair for VVF at our center from January 2012 to March 2018 was performed. VVFs were repaired by a suprapubic transvesical approach using a homemade laparoscopic single-port device. Clinical data, perioperative data and outcomes were collected. The primary outcome was VVF successful closure rate, and secondary outcome was perioperative complications.Results: The mean age of the patients was 44.6 (27–58) yr. The mean follow-up time was 65.6 (32–118) mo. The VVFs were successfully closed in 37 (88.1%) patients after the first surgery, and failure was observed in five patients. Initial failures of all the five patients were cured after a second repair. No major complication occurred as defined by Clavien-Dindo class 2 or greater.Conclusions: Suprapubic transvesical approach to repair VVFs using a homemade laparoscopic single-port device is a simple, effective, and feasible approach offering ideal results without major complications.