Holothuria Polii Research Articles

Inhibitory activity of sphingomyelin on hemolytic activity of coelomic fluid of [formula omitted][formula omitted] (echinodermata)

The hemolytic activity of coelomic fluid from Holothuria polii is specifically inhibited by sphingomyelin. This phospholipid is the constituent of the membrane which probably interacts with the hemolysin thereby leading to the lysis.

Evolution of the lytic system in echinoderms. I. Naturally occurring hemolytic activity inParacentrotus lividus(Echinoidea) coelomic fluid

Abstract The hemolytic activity of the coelomic fluid of Paracentrotus lividus against vertebrate erythrocytes was investigated. It was found that this activity corresponds to a thermo‐labile, calcinum‐dependent protein. The chemico‐physical and biological properties of this protein are compared to those of the counterparts in other echinoderm hemolytic systems. No immunological cross‐reaction could be demonstrated between P. lividus and Holothuria polii hemolysin.

(F8) Membrane damage by hemolysin from Holothuria polii (Echinodermata) : C. Canicatti. Institute of Zoology. University of Palermo.

(F8) Membrane damage by hemolysin from Holothuria polii (Echinodermata) : C. Canicatti. Institute of Zoology. University of Palermo.

Chromatographic separation of coelomic fluid from Holothuria polii (echinodermata) and partial characterization of the fractions reacting with erythrocytes.

Coelomic fluid preparations from Holothuria polii were passed through a Bio-gel A5m column. The 3 separated protein peaks possess hemagglutinating or hemolytic activity against rabbit erythrocytes. Electrophoretic and immunochemical methods showed that 2 identical protein subunits characterized hemagglutinins of different size. Hemolysin differs from hemagglutinin in molecular weight and organization of subunits.

Visualization of native chromatin fibers in different embedding media

The influence of the embedding medium is of critical importance in the observation of nucleoprotein structures in thin sections. The use of conventional epoxi media requires dehydratation, impregnation and subsequent polymerization, which may produce drastic alterations in the fine structure of chromatin. The use of water soluble embedding media may lead to improvements in the preservation of ultrastructure. In this paper we explore the differences which may be detected in nucleohistone fibers embedded in different media.Spermatozoa from Holothuria polii were subjected to moderate lysis in either 0.15M NaCl, ImM Tris-HCl, 0.4mM CaCl2, pH 8.0 (a) or 0.25M sucrose, 0.4mM CaCl2 (b) and then fixed by addition of 2 % glutaralde- hyde. Embedding was carried out as described in the literature in the following media: araldite-epon mixture (AREPO), glycol methacrylate (GMA, 1), hydroxipropyl methacrylate (HPMA, 2) and bovine serum albumin (BSA, 3) or histones.

Untersuchungen über die Zusammensetzung der Desoxyribonucleinsäure aus den reifen Spermatozoen von Seegurken

A method which serves to isolate the gonads from the sea cucumber (Holothuria polii) is outlined. Criteria that will secure a well determined status of maturity of the sperm are given. From this preparation a deoxyribonucleic acid is made, purified and analysed. It is concluded that the analytical data are in compliance with the theory of Crick and Watson. The ratio of Moles for this DNA while its nitrogen to phosphorus ratio on weight basis is 1,67.