History Of Recurrent Miscarriage Research Articles

Anxiety, depression and social support in pregnant women with a history of recurrent miscarriage: a cross-sectional study

ABSTRACT Objective: This study aims to investigate the prevalence of anxiety and depressive symptoms and the associations between social support and anxiety/depressive symptoms amongst Chinese pregnant women with a history of recurrent miscarriage. Methods: A cross-sectional study was conducted in Guangzhou, China, between September 2016 and May 2017 with 278 Chinese pregnant women with a history of recurrent miscarriage. Measures included the Self-rating Anxiety Scale (SAS), the Edinburgh Postnatal Depression Scale (EPDS) and the Perceived Social Support Scale (PSSS). Results: The occurrence of anxiety (SAS ≥ 50) and depressive symptoms (EPDS ≥ 13) were 45.0% and 37.0%, respectively. The women reported moderate level of social support. The low and moderate level of social support and education of high school or below were the predictors for anxiety and depressive symptoms. The other predictors for anxiety symptoms were having been married for ≥5 years and no child. Another predictor for depressive symptoms was aged ≥35 years. Anxiety predicted depressive symptoms. Conclusion: The results suggest early routine screening for anxiety and depressive symptoms amongst the Chinese pregnant women with a history of recurrent miscarriage. Health-care professionals should give more effort to enhance these women’s social support to decrease their anxiety and depressive symptoms.

A Randomized Trial of Progesterone in Women With Bleeding in Early Pregnancy

(Abstracted from N Engl J Med 2019;380:1815–1824) The hormone progesterone plays a critical role in preparing the endometrium for implantation of the embryo and continues to be produced throughout pregnancy. Historically, progesterone supplementation has been initiated during early pregnancies to prevent miscarriages in asymptomatic women who have a history of recurrent miscarriages or to rescue a pregnancy in women who have started to bleed during early pregnancy.

2. CLINICAL COMPARISON OF TWO PGT-A PLATFORMS UTILIZING DIFFERENT THRESHOLDS TO DETERMINE PLOIDY STATUS

Introduction Unlike previous technologies that lack the diagnostic performance to reliably detect mosaicism for preimplantation genetic testing for aneuploidy (PGT-A), next generation sequencing (NGS) based platforms now allow for the detection of lower-level mosaicism. Due to this increased sensitivity, thresholds need to be put in place to classify embryos as euploid, aneuploid, or mosaic. The lower cutoff for mosaic samples ranges from 20 % to 30 %, and the upper cutoff for mosaic samples ranges from 70%-80% in most commercial PGT laboratories in the field today. These varying cutoffs will lead to varying percentages of mosaic embryos reported, suggested to be somewhere between 20 %. A more stringent threshold could result in greater pregnancy rates. Here we compare clinical outcomes and embryo reporting between two different PGT-A reference laboratories that use different testing platforms and different mosaic cutoffs. Materials and Methods Retrospective analysis of PGT-A results (n=623) and frozen embryo transfer (FET) cycles (n=99) using embryos diagnosed by either Cooper Genomics or Igenomix between January 2018 - January 2019. Cooper Genomics uses Illumina's MiSeq platform with Bluefuse software and thresholds of 20% to 80 % (lower to upper cutoff) while Igenomix uses ThermoFisher's Ion Torrent platform with Ion Reporter software and thresholds of 30% to 70 %. Patients with a history of recurrent miscarriage were excluded from this study. Results Fifty six (56) cycles included embryos analyzed by Cooper Genomics and 43 cycles included embryos analyzed by Igenomix as a PGT-A reference laboratory. There was no differences in maternal age or number of embryos replaced per transfer between the two cohorts. IVF outcome was similar between the two groups: The pregnancy (+FHB) observed in cycles that transferred embryos diagnosed as euploid by Cooper Genomics was 67.9% (38/56) compared to 69.8% (30/43) with Igenomix. Miscarriage rate was also similar, 8.9% (5/56) and 6.9% (3/43) respectively. Cooper Genomics reported a much higher rate of mosaicism (17.0%) compared to Igenomix (4.9%) (p Conclusion Retrospective analysis of PGT-A+FET cycles revealed there was no differences in pregnancy or miscarriage rates indicating a similar diagnostic efficacy achieved by the two PGT-A platforms. Although the embryos diagnosed as euploid by the two platforms appeared to have the same potential to generate a pregnancy, the methodology and higher threshold utilized by Igenomix resulted in significantly less mosaic embryos and a slightly greater percentage of euploid embryos available for transfer. It is assumed that cumulative pregnancy rates between the two reference laboratories would vary based on the finding that there were more euploid embryos available from Igenomix. Results from subsequent FET cycles will be collected and further examined. NGS has now created three categories of embryos that exist after PGT-A, the interpretation of the millions of data points generated by this technology still remains a challenge and it is important that the diagnostic methodology used does not overestimate the degree of mosaicism.

Changes in expression of Ki-67, CD16 and CD56 by natural killer cells from peripheral blood mononuclear cells in the setting of recurrent miscarriage after in vitro culturing in the presence of trophoblast cells and IL-2.

The aim of this research was to assess the proliferative activity of Natural Killer Cells (NK cells) from Peripheral Blood Mononuclear Cells (PBMCs) in the presence of trophoblast cells in women with a history of recurrent miscarriages. We examined the peripheral blood of women with recurrent miscarriage in the proliferative (n = 12) or secretory (n = 13) phase of their menstrual cycle, and pregnant women with a history of recurrent miscarriage at 6-7weeks of their current pregnancy (n = 14). Controls were fertile non-pregnant women in the proliferative (n = 11) or secretory (n = 13) phase of their menstrual cycle, and pregnant women at 6-7weeks of a physiologically normal pregnancy (n = 20). We used IL-2 as a factor maintaining PBMCs viability during long-term culturing. We established that culturing in the presence of IL-2 contributed to an increase in the number of CD56+CD16- NK cells and to a decrease in the number of CD56+CD16+ NK cells from PBMCs compared with these numbers before culturing in both healthy women and in women with recurrent miscarriage. After culturing of PBMCs in the presence of trophoblast cells and IL-2 (compared with culturing without trophoblast cells), the intensity of Ki-67 expression by NK cells was reduced in the whole NK cell population (CD3-CD56+), and in the CD56+CD16- and CD56+CD16+ populations of NK cells in women with recurrent miscarriage and in healthy controls. The intensity of CD56 expression was reduced in the presence of trophoblast cells and IL-2 in non-pregnant women with recurrent miscarriage in the secretory versus the proliferative phase of the menstrual cycle.

Combined presence of coagulation factor XIII V34L and plasminogen activator inhibitor 1 4G/5G gene polymorphisms significantly contribute to recurrent pregnancy loss in Serbian population.

Recurrent pregnancy loss (RPL) is a heterogeneous condition affecting up to 5% of women of reproductive age. Inherited thrombophilia have been postulated as one of the causes of RPL. Here we examined the prevalence of nine thrombophilic gene polymorphisms among women with history of recurrent miscarriages and fertile controls. The study included 70 women with history of at least three early pregnancy losses and 31 fertile controls with no miscarriages. We investigated mutations in genes responsible for clotting and fibrinolysis, including factor V (FV) Leiden, FV H1299R, factor II (FII) G20210A, methylene tetrahydrofolate reductase (MTHFR) C677T and A1298C, factor XIII (FXIII) V34L, plasminogen activator inhibitor-1 (PAI-1) 4G/5G and endothelial protein C receptor (EPCR) H1 and H3 haplotypes using reverse polymerase chain reaction ViennaLab cardiovascular disease StrippAssays. Our results showed no significant increase in prevalence of tested polymorphisms in women with RPL. However, relative risk for PRL among women heterozygous for FXIII V34L was 2.81 times increased (OR 2.81, 95% CI 1.15-6.87, P=0.023). Haplotype analysis showed that combined presence of high-risk genotypes for FXIII and PAI-1 significantly increases risk for RPL (OR 13.98, CI 95% 1.11-17.46, P=0.044). This is the first study in Serbian population that investigated prevalence of FVR2, A1298C, FXIII V34L and EPCR gene variants. Compound heterozygosity for FXIII V34L and PAI-1 4G is significant risk factor for recurrent miscarriage. Our results should be viewed in context of small case-control study, so further large prospective studies are need for confirmation of our findings.

Clinicopathological characteristics of miscarriages featuring placental massive perivillous fibrin deposition

IntroductionMassive perivillous fibrin deposition (MPFD) is frequently associated with detrimental pregnancy outcomes, and extensive perivillous fibrin deposition results in severe placental dysfunction and loss of maternofetal interface. Unfortunately, the fundamental pathogenesis of MPFD remains unknown, and systematic analyses of MPFD in miscarriage is lacking. We analyzed the frequency and clinicopathological characteristics of MPFD in first trimester miscarriages. MethodsWe analyzed a consecutive series of miscarriages (n = 582) gathered between March 2012 and June 2016. MPFD was classified as fibrin-type (f-MPFD) and matrix-type (m-MPFD) by immunostaining for fibrin and collagen type IV. The control group consisted of miscarriage cases (MC, n = 18) that were matched to f-MPFD with normal chromosome (f-MPFD-nc) for number of previous miscarriages and placental chromosomal status. ResultsMPFD was identified in 2.7% of miscarriages. f-MPFD was associated with recurrent abortions. Compared with miscarriages without fibrin deposition, MPFD cases had higher proportion of those with normal placental chromosome (69.2% vs. 27.4%, P < 0.005) and higher frequency of villous syncytiotrophoblast C4d deposition (73.3% vs. 33.9%, P < 0.005). All C4d(+) f-MPFD patients had more than three recurrent miscarriages, whereas C4d(−) f-MPFD patients had no history of recurrent miscarriage (P < 0.05). Patients with f-MPFD-nc had significantly higher HLA PRA immunopositivity rate than did MC patients (P = 0.005). DiscussionMPFD was more common in miscarriages than in preterm and term pregnancies. Placental massive fibrin-type fibrinoid deposition and villous C4d immunoreactivity were associated with recurrent miscarriage.

Elevated level of antisperm antibodies as a risk factor for unfavorable pregnancy outcome after use of assisted reproductive technology

The study objective is to analyze the connection between the level ofantisperm antibodies and pregnancy outcomes in pairs who underwent in vitro fertilization procedure due to secondary infertility. Materials and methods . At the center for family planning and reproduction V.N. Gorodkov Ivanovo Scientific Research Institute for Maternity and Childhood, a prospective cohort study of 46 women with a history of recurrent miscarriage and secondary infertility developed in the last year which became an indication for use of assisted reproductive technologies was performed. A comparative analysis of spermograms and MAR test results prior to ART was performed. The group 1 included pairs with MAR test result &gt;10 %, the group 2 — MAR test result &lt;10 %. Results. Forty-one percent (41 %) of pregnancies in the group 1 and 82.3 % of pregnancies in the group 2 resulted in term normal birth (p &lt;0.05). A moderate positive correlation (r = 0.36) between elevated IgG level and unfavorable pregnancy outcome (miscarriage) was observed. Elevated level of antisperm antibodies increased the risk of miscarriage by 6.57 (1.67—25.78). Conclusion. Presence of antisperm antibodies on the surface of spermatozoa dramatically worsens pregnancy outcome prognosis even if the number of covered spermatozoa is between 10 and 49 %. This characteristic can be used as a predictor for reproductive failure.

Relative Expression of OX40, OX40L mRNA, and OX40L Serum Levels in Women with Recurrent Spontaneous Abortion

ABSTRACTThis study determined the roles of OX40 and OX40L in women with recurrent spontaneous abortion (RSA). We compared the expression of OX40 and OX40L genes in peripheral blood mRNA levels and serum levels of OX40L in women with a history of RSA to the control group. In this case-control study, 40 women with a history of RSA (case group), and 40 others with no history of abortion (control group) were investigated. The expressions of OX40 mRNA and OX40L mRNA were determined in the two groups using the quantitative polymerase chain reaction. Also, the enzyme-linked immunosorbent assay was used to determine the levels of serum OX40L in the two groups. There were no significant differences in the maternal age of women in the two groups (30.1 ± 4.28 years in the case vs. 30.03 ± 4.23 years in the control group). There was no difference in terms of the levels of OX40 and OX40L mRNA between the groups (p = 0.08 and p = 0.56, respectively). In addition, there was no significant correlation between the expression of OX40 and OX40L mRNA levels with age or the number of abortions. The correlation between OX40 and OX40L mRNA levels was not significant. RSA history group turned to show a higher level of serum OX40L than the control group (p = 0.03). In conclusion, our findings demonstrated that the expression of OX40 mRNA and OX40L mRNA was similar between women with a history of RSA and the control group. The elevation of serum OX40L level may be considered as a risk factor for RSA.

Increased HLA-G Expression in Term Placenta of Women with a History of Recurrent Miscarriage Despite Their Genetic Predisposition to Decreased HLA-G Levels.

Human leukocyte antigen (HLA)-G is an immune modulating molecule that is present on fetal extravillous trophoblasts at the fetal-maternal interface. Single nucleotide polymorphisms (SNPs) in the 3 prime untranslated region (3′UTR) of the HLA-G gene can affect the level of HLA-G expression, which may be altered in women with recurrent miscarriages (RM). This case-control study included 23 women with a medical history of three or more consecutive miscarriages who delivered a child after uncomplicated pregnancy, and 46 controls with uncomplicated pregnancy. Genomic DNA was isolated to sequence the 3′UTR of HLA-G. Tissue from term placentas was processed to quantify the HLA-G protein and mRNA levels. The women with a history of RM had a lower frequency of the HLA-G 3′UTR 14-bp del/del genotype as compared to controls (Odds ratio (OR) 0.28; p = 0.039), which has previously been related to higher soluble HLA-G levels. Yet, HLA-G protein (OR 6.67; p = 0.006) and mRNA (OR 6.33; p = 0.010) expression was increased in term placentas of women with a history of RM as compared to controls. In conclusion, during a successful pregnancy, HLA-G expression is elevated in term placentas from women with a history of RM as compared to controls, despite a genetic predisposition that is associated with decreased HLA-G levels. These findings suggest that HLA-G upregulation could be a compensatory mechanism in the occurrence of RM to achieve an ongoing pregnancy.

Recurrent pregnancy loss: can factor V Leiden mutations be a cause.

The role of Factor V Leiden (FVL) mutation in recurrent miscarriages has been disputed. It has been hypothesized that FVL mutation in patients with recurrent miscarriages is treatable. In this study, we evaluated 78 pregnant women for FVL mutations, among whom 50 had a history of recurrent miscarriages. Only 1 (2%) of the woman was positive for heterozygous FVL mutation. The incidence of FVL mutations in patients with recurrent pregnancy loss had an odds ratio of 1.72 (95% confidence interval, 0.0681–43.8257; P>0.05). However, the findings were not statistically significant. Thus, we suggest that FVL mutation study may not be included in the battery of tests for recurrent miscarriages in the Indian population.

Utilization of Whole Exome Sequencing in Lethal Form of Multiple Pterygium Syndrome: Identification of Mutations in Embryonal Subunit of Acetylcholine Receptor.

The acetylcholine receptor (AChR) is a member of the superfamily of transmitter-gated ion channels having a critical role in controlling electrical signals between nerves and muscle cells. Disruptive mutations in genes encoding different subunits of AChR result in multiple pterygium syndrome (MPS), which can be associated with a severe prenatally lethal presentation. This study aimed to investigate the etiology of lethal MPS (LMPS) in two consanguineous families with a history of miscarriages. DNA was extracted from a tissue sample of two aborted fetuses (probands) from two different families with a history of spontaneous miscarriages. Parental peripheral blood samples were collected for confirmatory analysis and follow-up testing. Whole-exome sequencing (WES) was performed on DNA from the probands. The results were confirmed and segregated by Sanger sequencing. Moreover, protein structure evaluations were accomplished. We identified a homozygous frameshift mutation of c.753_754delCT (p.V253fs*44) and a homozygous missense mutation of c.715C>T (p.Arg239Cys) in the CHRNG gene. Both aborted fetuses had pterygium, severe arthrogryposis, and fetal hydrops with cystic hygroma, being compatible with LMPS. The heterozygous state was confirmed in parents for both CHRNG variants. Likewise, CHRNG mutation was predicted to display the damaging effects by lowering the number of helixes and modifying the surface electrostatic potential. The present study identified rare sequence variants in the CHRNG gene in aborted fetuses from consanguineous couples with recurrent miscarriage history. WES is a comprehensive and cost-effective approach to study heterogeneous diseases including MPS. Such findings can improve our knowledge of MPS databases, particularly for genetic counseling of high-risk families and preimplantation genetic diagnosis.

Periodontitis and recurrent miscarriage: a case-control study of 41 women with medical history of recurrent miscarriage

Periodontitis and recurrent miscarriage: a case-control study of 41 women with medical history of recurrent miscarriage

Analysis of chromosome microstructures in products of conception associated with recurrent miscarriage.

The causes of almost half of all miscarriages are unknown. Genetic alterations undetectable by conventional methods may cause some cases of recurrent miscarriage. The study aimed to identify candidate genetic alterations associated with recurrent miscarriage. Twenty-nine Japanese women with a history of recurrent miscarriage without any known underlying anatomical or medical causes were recruited. The products of conception were collected after miscarriage and showed either a normal karyotype or a failure of complete chromosomal Giemsa banding. Genomic DNA from the chorionic villi of the conception products was analysed using genome-wide single-nucleotide polymorphism (SNP) arrays. In four cases, the products could not be analysed because of contaminating maternal-origin DNA, and chromosomal aneuploidies were observed in 10 cases. Thirty-three copy-number variations (CNV) were identified from the array data of 15 diploid cases. Causative CNV were identified by comparison with CNV observed in healthy, parous Japanese women. Twenty-four chromosomal regions with 26 CNV were identified as strong candidates for causing recurrent miscarriage, and these were all too small to detect by conventional chromosome analysis banding. Moreover, one novel CNV that caused complete deletion of a microRNA cluster region was detected. High-resolution genome-wide SNP arrays are effective for detecting novel genetic factors causing recurrent miscarriage. A more appropriate reference CNV list may be necessary to more effectively enrich for CNV likely to cause recurrent miscarriage. The findings confirmed one non-coding RNA cluster as a strong candidate that may contribute to unexplained miscarriages. Gene expression-regulatory mechanisms may play important roles in the pathogenesis of miscarriages.

Thyroid Autoimmunity is a Risk Factor for Recurrent Pregnancy Loss

This was a hospital based case control study. The study included patients attending in outdoor and indoor Department of Obstetrics and Gynaecology of Rajshahi Medical College, Hospital since July 2014 to June 2016. The aim of this study was to determine thyroid antibodies is a risk factor for recurrent pregnancy loss. Sixty seven pregnant or non pregnant women with history of recurrent miscarriage during 1st trimester were selected as case group and another sixty seven pregnant women who reached their 12 weeks uneventfully with no history of recurrent miscarriage were selected as control group. Patients with other cause of recurrent miscarriage such as metabolic or endocrinologic disorders, genital organ anomaly, uterine fibroid were excluded from the study. Thyroid function test and thyroid antibodies (FT4, TSH and TPO-Ab) were measured for the two groups. The result of this study showed that the percentage of positive TPO-Ab in target and control group is 67.16% and 5.95% respectively. The study observed that the mean serum concentrations of FT4 in the control subject was significantly higher than the mean of the target group (p-value &lt;0.05). The TSH concentration was increased in miscarriage women with positive antibodies compared with the concentration of TSH in the control group with positive antibodies. The conclusions are that there is a deficiency in thyroid hormones or thyroid’s functional capacity is unable to meet the extra demands of pregnancy which may be one of the causes of recurrent miscarriage. Moreover positive thyroid antibodies pregnant women can reach term and have babies when the concentration of TSH is low during the first trimester, so the risk of miscarriage could be high in positive thyroid antibodies.TAJ 2017; 30(1): 49-55

Analysis of BMP4 (rs121912765) polymorphism in Iranian women with history of recurrent spontaneous abortion: A case-control study.

Recurrent spontaneous abortion (RSA) defines as the consecutive loss of at least two pregnancies prior to the 20th week of gestation. A qualitative diagnosis of infertility can only be performed by focusing on female and male physical abnormalities, endocrine irregularities and genetic conditions. The aim of the present study was to evaluate the association between a common polymorphism of bone morphogenetic protein 4 (BMP4) (rs121912765; 278A>G) with female infertility. At present there is a lack of data on the relevance of BMP4 polymorphism to spontaneous abortion among Iranian subjects. In the present case-control study, the BMP4 (rs121912765) polymorphism was investigated in 70 infertile women and 100 healthy subjects from Iran by polymerase chain reaction-restriction fragment length polymorphism analysis. Odds ratios (ORs) and 95% confidence intervals (CIs) for the association between BMP4 (rs121912765) polymorphism and risk of recurrent abortion risk in Iranian women were determined using binary logistic regression, and the genotype and allele frequencies were compared using the χ2 test. Results indicated significant association between the rs121912765 polymorphism and recurrent spontaneous abortion. The χ2 test indicated that the allele frequencies differed (OR=0.071, 95%CI=0.022-0.181; P<0.0001), with the A allele appearing more prevalent in the case samples. Therefore, this polymorphism may be a useful genetic marker for diagnosing female infertility in the context of RSA. The study of such a polymorphism can provide an important basis for targeting interventions and prevention in high-risk individuals. Therefore, further studies are necessary to establish the association of BMP4 (rs121912765) polymorphism in larger and more diverse populations.

Состояние биоценоза у беременных женщин с привычным невынашиванием в анамнезе

Состояние биоценоза у беременных женщин с привычным невынашиванием в анамнезе

Subsequent pregnancy and perinatal outcome in women with a history of recurrent miscarriage: a systematic review and meta-analysis

BackgroundWomen with a history of recurrent miscarriage are accorded dedicated medical care during a subsequent pregnancy (or subsequent pregnancies). Previous studies of subsequent pregnancy and perinatal outcome in women with a history of recurrent miscarriage give conflicting results, with some reporting an increase in adverse obstetric and perinatal outcomes, and others reporting no difference. There is a lack of systematic review based on a large-scale population. MethodsWe carried out a systematic review and meta-analysis to incorporate direct and indirect data from relevant studies. We searched PubMed, Scopus, Embase, Web of Science, and Ovid, up to Dec 31, 2017. Studies that were included were full text reports of pregnancy outcomes in women with a history of recurrent miscarriage. We used the random-effect model or the fixed-effect model to estimate the incidence of different obstetric and perinatal outcomes. We also carried out subgroup analysis to determine the effect of the sex of the fetus on the incidence of different obstetric and perinatal outcomes. FindingsAmong 1842 full articles retrieved, 7 articles with a total of 224 879 participants, including 10 174 women with a history of recurrent miscarriage and 21 4705 control subjects, were included in this systematic review and meta-analysis. Recurrent miscarriage was associated with a significantly increased incidence of antepartum haemorrhage (odds ratio [OR] 2·38, 95% CI 1·98–5·27), gestational diabetes (1·23, 1·02–1·47), preterm delivery (1·73, 1·28–2·07), small for gestational age (1·52, 1·19–1·98), and perinatal death (2·30, 1·62–4·97). There was no significant difference in the incidence of gestational hypertension, or in Apgar scores at 1 and 5 minutes after birth. There was also no significant difference in risk between male fetus and female fetus. InterpretationWomen with a history of recurrent miscarriage have an increased risk of several obstetric and perinatal complications, and therefore may require more dedicated medical care in their subsequent pregnancies. FundingThis work was supported by the Health and Medical Research Fund in Hong Kong (number 04152786) and the Hong Kong Obstetrical and Gynaecological Trust Fund in 2017.

The Effects of Nitric Oxide Patch Versus Oral Sildenafil Citrate on First trimester Pregnant Women with a History of Recurrent Miscarriage

The Effects of Nitric Oxide Patch Versus Oral Sildenafil Citrate on First trimester Pregnant Women with a History of Recurrent Miscarriage

Prevalence of hypothyroidism in case of repeated miscarriages in the first trimester of pregnancy

Purpose To determine the frequency of hypothyroidism in women with recurrent pregnancy loss in the first trimester of pregnancy. Materials and methods The study included 163 pregnant women with no previous history of recurrent miscarriage in a gestational age Results Hypothyroidism was found in 7 cases in group 1 (4.12 %) and in one case in the control group. The differences in serum levels of T3, T4 and TSH were significant in favor of group 1. Conclusion The study shows that hypothyroidism has a statistically significant relationship with recurrent miscarriage in the first trimester and suggests that the diagnosis of hypothyroidism should be carried out systematically in the etiological investigation of this pathology.

Cytogenetic abnormalities in couples with a history of primary and secondary recurrent miscarriage: a Brazilian Multicentric Study

Objective: To evaluate the difference between chromosomal abnormalities between the gender of couples affected by Recurrent miscarriage (RM) and if there is an association between previous obstetric history and chromosomal abnormalities of the parents.Methods: Multicenter, retrospective, observational study from seven different RM clinics between 2006 and 2016. We enrolled 707 couples (1014 participants) with a history of RM. We compared the frequency of chromosomal abnormalities between groups of couples with primary and secondary RM and separated between women and their partners. Furthermore, we compared the prevalence of chromosomal abnormalities between groups based on the number of previous spontaneous abortions.Results: The overall prevalence of all cytogenetic abnormalities was 5.59% (n = 1414, women and their partners). Excluding cases of polymorphism and inversion of chromosome 9, which are considered variants of normality, the prevalence in all individuals was 2.26% (n = 32/1414). The comparative analysis of cases of chromosomal abnormalities among couples with primary and secondary RM based on the number of previous miscarriages (PM) revealed a similar frequency between groups. The statistical analysis of the total cases (primary PM + secondary PM) in these three groups were as follows: (a) couple, 2 pm versus 3 pm vs. ≥4 PM, p = .514; (b) women, 2 pm versus 3 pm vs. ≥4 PM, p = .347; and (3) partner, 2 pm versus 3 pm vs. ≥4 PM, p = .959. Chromosomal abnormalities were significantly more prevalent among women than among their partners (6.9 versus 4.2%; p = .027). Moreover, the distribution of leading chromosomal abnormalities among women was different compared with their partners. Among women, we observed these abnormalities in the following frequency order: mosaicism (38.8%), polymorphism (32.6%), translocation (16.3%), and inversion (12.3%). Among their partners, these abnormalities were polymorphism (73.3%), inversion (13.3%), mosaicism (6.7%), and translocation (6.7%).Conclusion: The number of PM and the history of full-term pregnancy does not correlate with an increase or decrease in the prevalence of cytogenetic abnormalities in couples with RM.