Historical Mining Activities Research Articles

Historic Underground Silver Mine Workings Detection Using 2D Electrical Resistivity Imaging (Durango, Mexico)

This paper presents an underground silver mining operation outside Gomez Palacio, Durango, Mexico, terminated around the 1930s, of which previous knowledge of its operations was poor. Durango’s current silver exploration campaigns are likely to overlook historic silver mining sites due to interest in specific prospect regions. A two-dimensional (2D) Electrical Resistivity Imaging (ERI) survey coupled with reconnaissance of the area was performed at this historic silver mining site. The exploration campaign aimed to find the abandoned mineshaft, map its subsurface extent, and explore the occurrence of mineralization zones (silver ore). The ERI survey comprised five profiles measured with the extended dipole-dipole array with a consistent electrode spacing of 5 m. The smooth, robust, and damped least-squares inversion methods were used to invert the 2D data. Our field observations and ERI survey results collectively reveal the following findings: (a) reconnaissance reveals mining infrastructure consistent with historical mining activity; the infrastructure includes a complex of habitational rooms, an ore-processing pit near a concrete slab next to a dirt road, and two limestone-wall structures interpreted as the entrance of abandoned backfilled mineshafts named Mesquite and Lechuguilla; (b) high-resistivity anomalies suggest vestiges of shallow, underground mine workings including backfilled mineshafts that connect a mine gallery complex, and (c) various low-resistivity anomalies, juxtaposed against mine galleries, suggestive of unmined shallow vein-type and manto-type mineralization of hydrothermal origin. The imaging depth is estimated at ~65 m. Underground silver mining moved southwards and was limited to ~40 m depth.

Antimony and arsenic particle size distribution in a mining contaminated freshwater river: Implications for sediment quality assessment and quantifying dispersion.

Assessments of antimony (Sb) and arsenic (As) contamination in sediments are reported on a wide range of different particle size fractions, including <63 μm, < 180 μm and <2 mm. Guidelines vary between jurisdictions which limits comparative assessment between contamination events and complicates ecotoxicity assessment, and almost no information exists on Sb size distribution in contaminated sediments. This study quantified and compared the size distribution of Sb and As in 11 sediments (and 2 floodplain soils) collected along 320 km of waterway contaminated by historic mining activity. Sediment particle size distribution was the primary determinant of total metalloid load in size fractions across the varying substrates of the waterway. Minerals and sorption complexes influenced metalloid particle distribution but relative importance depended on location. Arsenic concentrations were greatest in the fine <63 μm fraction across all the different river environments (7.3–189 mg kg−1, or 1–26% of total sample As), attributed to fine-grained primary arsenopyrite and/or sorption of As(V) to fine solid-phases. The Sb particle size concentrations were greatest in mid-size fractions (205–903 mg kg−1) in the upper catchment and up to 100 km downstream to the mid-catchment as a result of remnant Sb minerals. Antimony concentrations in the lower catchment were greatest in the <63 μm fraction (8.8–12.1 mg kg−1), reflecting the increasing importance of sorption for Sb particle associations. This work demonstrates the importance of particle size analysed for assessment of sediment quality, and provides support for analysis of at least the <250 μm fraction for Sb and As when comparing pollutant distribution in events impacted by primary contamination. Analysis of the <63 μm fraction, however, provides good representation in well-dispersed contaminated sediments.

Quantitative Analysis and Human Health Risk Assessment of Heavy Metals in Paddy Plants Collected from Perak, Malaysia.

Rice is one of the major crops as well as the staple food in Malaysia. However, historical mining activity has raised a concern regarding heavy metal contamination in paddy plants, especially in Perak, a state with major tin mining during the late nineteenth century. Therefore, the objective of this study is to investigate the heavy metals (As, Cd, Pb, Cu, Cr) contamination in paddy soils and paddy plants in three districts in Perak. The content of heavy metals was determined using ICP-MS, while the absorption and transferability of heavy metals in the paddy plants were investigated through enrichment (EF) and translocation (TF) factors. Principal component analysis (PCA) was employed to recognize the pattern of heavy metal contaminations in different sampling areas. Health risk assessment was performed through calculation of various indices. The quantification results showed that root contained highest concentration of the studied heavy metals, with As exhibiting the highest concentration. The EF results revealed the accumulation of As, Cu, and Cr in the rice grains while PCA showed the different compositional pattern in the different sampling areas. The health risk assessment disclosed both noncarcinogenic and carcinogenic risks in the local adults and children. Overall, findings from this study show that heavy metal contamination poses potential health risks to the residents and control measure is required.

Medieval Mining Centre of Buchberg in the Bohemian-Moravian Highlands. Metal Production in the Kingdom of Bohemia (13th–14th Centuries)

The study presents the results of a wide range of research approaches and surveys of the defunct mining centre at Buchberg in the region of Havlíčkův Brod, which was involved in silver production in the 13th and 14th centuries. Burchberg is exceptional in its size and well-advanced community infrastructure. Its significance is also reflected in written sources. Surveys and trial diggings focused on the residential area, and especially on the adjoining metallurgical facilities providing unique spatial information, as well as a wealth of valuable data obtained by analysing archaeometallurgical materials, representing the links in the operational chain, staring with the raw ore extraction and ending with the final production of the desired metals. The current deforestation of mining field relics enabled their three-dimensional survey, and, thus, providing a hitherto unknown view of the spatial structure of historical mining activities.

Vegetation and Environmental Changes on Contaminated Soil Formed on Waste from an Historic Zn-Pb Ore-Washing Plant.

Simple SummaryRemnants of former Zn-Pb mining in southern Poland are an important element of geographical space. Some of the post-mining areas have found new economic and residential functions. Many of them are undergoing ecological succession and constitute valuable natural habitats enriching biodiversity of the surrounding landscapes. There are places where we can observe and document various ecological and geochemical transformations in its historical and contemporary aspects. These changes provide a basis for observing and functioning of ecosystems developing in an area transformed under the influence of Zn-Pb mining.Post-mining waste from Zn-Pb ore exploitation undergoes processes of spontaneous succession and changes in soil chemical composition. The Zakawie area was industrially transformed by historical mining activity, ore enrichment, and the metallurgical processing of Zn-Pb ore. The subject of the study was to analyse the rate of vegetation succession (from 1999 to 2019), soil chemistry, and the relationships between them in an anthropogenic habitat with high concentrations of potentially toxic metals. Ecological and geochemical studies were carried out in an area contaminated with waste from a disused Zn-Pb ore-washing plant. Between 1999 and 2019, the transformation of grassland and meadow vegetation into scrub and forest–grassland communities was observed. This transformation led to a decrease in the area of Molinietum caeruleae meadow (from 25.8% in 1999 to 10.7% in 2019), whose place was taken by Prunus spinosa and Rhamnus cathartica. The community of xerothermic limestone grasslands completely disappeared, being replaced in favour of the Diantho-Armerietum and Prunus spinosa community. In this period, the share of lifeforms of plants and species composition (46 and 60, respectively) also changed. The Shannon and Simpson biodiversity index reached high values in the second investigation period, and it was 0.893 and 0.86, respectively. The anthrosols had a high content of Zn—85,360 mg kg−1, Pb—28,300 mg kg−1, Cd—340 mg kg−1, and As—1200 mg kg−1. Carbonates, clay minerals, and fe-oxides are predominant in the mineral composition of the rhizosphere; the metal-bearing phases are stable; and hardly soluble minerals include smithsonite, cerussite, monheimite, hemimorphite, and oxides of Fe and Fe-Mn. Mineralisation/crust processes formed on the epidermis, and their influences on root development were found. Scanning electron microscopy and energy-dispersive X-ray spectroscopy studies on rhizosphere soil components provide information on the type of minerals and their susceptibility to heavy metals release. The identification of some biotic and mineral structures in rhizospheres can be an interesting source of information on pedogenic processes identified in back-scattered electron images.

Heavy metal concentrations in floodplain soils of the Innerste River and in leaves of wild blackberries (Rubus fruticosus L. agg.) growing within and outside the floodplain: the legacy of historical mining activities in the Harz Mountains (Germany)

We studied heavy metal levels in floodplain soils of the Innerste River in northern Germany and in the leaves of wild blackberries (Rubus fruticosus L. agg.) growing within and in adjacent areas outside the river floodplain. Heavy metal contamination of the Innerste floodplain is a legacy of historical metal ore mining, processing, and smelting in the Harz Mountains. The heavy metal (Cd, Pb, Zn, Cu, Ni, and Cr) contents of previously studied soil samples from eleven floodplain sites along the Innerste River were re-analyzed statistically, and the levels of these metals in blackberry leaves were determined at five sites. Mean concentrations in the floodplain soils were elevated by factors of 4.59 to 28.5 for Cd, 13.03 to 158.21 for Pb, 5.66 to 45.83 for Zn, and 1.1–14.81 for Cu relative to the precautionary limits for soils stipulated by the German Federal Soil Protection and Contaminated Sites Ordinance. Cadmium, Pb, Zn, Cu, and Ni levels in floodplain soils decreased markedly downstream, as did the concentrations of Cd, Zn, and Ni in the leaves of blackberries from within the floodplain. Levels of Cd, Pb, and Zn in leaves of blackberries from within the floodplain significantly exceeded those of specimens from outside the floodplain. The findings of our study highlight the potential of wild blackberry as a biomonitor of soil pollution by Cd, Pb, and Zn and corroborate the massive heavy metal contamination of floodplain soils along the Innerste River observed in previous studies.

Solid-Phase Partitioning and Leaching Behavior of Pb and Zn from Playground Soils in Kabwe, Zambia.

Zambia’s Kabwe mine wastes (KMWs) are responsible for contaminating the surrounding soil and dust in the Kabwe district. Unfortunately, these wastes arise from the historical mining activities of lead (Pb) and Zinc (Zn), which lacked adequate waste management strategies. As a result, potentially toxic elements (PTEs) (Pb and Zn) spread across the Kabwe district. To assess the soil pollution derived from previous mining activities, we studied topsoil samples (n = 8) from the school playground soils (SPs). In this study, the degree of contamination, geochemical partitioning, and leachability, coupled with the release and retention of Pb and Zn, were studied. The SPs were classified as extremely enriched (EF > 40) and contaminated with Pb (Igeo > 5). On average, Pb (up to 89%) and Zn (up to 69%) were bound with exchangeable, weak acid-soluble, reducible and oxidizable phases, which are considered as ’geochemically mobile’ phases in the environment. The leachates from the soils (n = 5) exceeded the Zambian standard (ZS: 190:2010) for Pb in potable drinking water (Pb < 0.01 mg/L). Furthermore, the spatial distribution of Pb and Zn showed a significant reduction in contents of Pb and Zn with the distance from the mine area.

Long-term Landsat monitoring of mining subsidence based on spatiotemporal variations in soil moisture: A case study of Shanxi Province, China

Ground subsidence caused by mining activities has extensively and chronically affected people living near mining areas. Remote sensing images provide temporally and spatially continuous observations of surface natural resources. In order to accurately understand and continuously observe subsidence areas, this study investigated the spatiotemporal dynamics of mining subsidence using long-term Landsat time-series. The study area was a typical mining region in Shanxi Province, China, with a long history of mining activity. Landsat images from 1986 to 2019, as well as reference data, were used to construct inter-annual time-series trajectories of soil moisture, which were derived from spatial normalizations of the soil moisture monitoring index(SSMMI). The LandTrendr algorithm on the Google Earth Engine platform was then used to extract the subsidence areas and their spatiotemporal dynamics. The results indicate that (i)the map of the spatial distributions of mining subsidence had an overall accuracy of 83.8% and indicate that areas that underwent subsidence were mainly concentrated in mining cites(including Taiyuan, Lvliang, and Linfen); (ii)subsidence before 1990 accounted for 39.6% of the total occurrences and a large proportion(45.18%) of the subsidence was short-term; (iii)subsidence in mountain areas that have long histories of mining and long subsidence durations require further attention by researchers. This study allows us to advance the long-term monitoring of mining subsidence times using variations in soil moisture, combined with moderate resolution satellite data such as Landsat and time-series analysis algorithms.

Do Old Mining Areas Represent an Environmental Problem and Health Risk? A Critical Discussion through a Particular Case

A bibliographic review was carried out to establish the state of knowledge of a mining area with several centuries of exploitation and currently abandoned. The selected case study, the Sierra Minera de Cartagena-La Union (Spain), has a long history of mining activity, ending in 1990. The area is rich in metallic sulphide (lead, zinc and iron), with underground mines and quarries. The zone is very close to important populations and affects protected sites of special ecological value. It is also adjacent to areas dedicated to agriculture and important centres of tourist interest. It is a territory that meets the requirements to be classified as a critical area, as it is in a state of unstable physical and geochemical equilibrium, giving rise to possible risks to human health and ecosystems. A literature review was carried out according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) methodology criteria, consulting a large number of related publications. The results obtained using the Source-Pathway-Receptor model make it possible to identify the main impacts caused by the contamination sources, the main routes of contamination, as well as the transfer to the biota and the influence on adjacent agricultural soils. In this study, lead, cadmium, zinc, arsenic, copper and manganese were considered as potential toxic elements (PTEs), and data were obtained on concentrations in soil, water and air as well as in fauna and flora. Finally, once the receptors and the associated risks to the ecosystem and human health were identified, a conceptual model of the contamination was drawn up to consider a management proposal to tackle the problems associated with this area, which would also be applicable to critical mining zones.

Assessing the potential environmental factors affecting cladoceran assemblage composition in arsenic-contaminated lakes near abandoned silver mines

Silver mining has a long history in Cobalt (Ontario, Canada), and it has left a complex environmental legacy where many lakes are contaminated with arsenic-rich mine tailings. In this exploratory survey, we examined subfossil Cladocera remains in the surface sediments of 22 lakes in the abandoned mining region to assess which environmental variables may be influencing the recent assemblage structure. Further, using a “top-bottom” paleolimnological approach, we compared the recent (top) and older (bottom) assemblages from a subset of 16 lakes to determine how cladoceran composition has changed in these lakes. Our regional survey suggests that the cladoceran assemblages in the Cobalt area are primarily structured by differences in lake depth, while site-specific limnological characteristics, including those related to past mining activities, may have limited roles in shaping the recent cladoceran compositions. The top-bottom paleolimnological analysis suggests that the cladoceran assemblages have changed in most lakes around Cobalt, however the magnitude and nature of changes varied across the study sites. As with most regional biological surveys, the responses to historical mining activities were not uniform across all sites, which further emphasizes the importance of considering site-specific limnological characteristics and multiple environmental stressors when assessing the impacts of mining pollution.

Legacy of Past Mining Activity Affecting the Present Distribution of Dissolved and Particulate Mercury and Methylmercury in an Estuarine Environment (Nalón River, Northern Spain)

At the Nalón River estuary (Asturias, Northern Spain), the occurrence of Hg is due to historical mining activity which has resulted in environmental issues of great concern. Although several studies have investigated the sediment compartment regarding Hg contamination, no information is currently available on the fate of Hg and MeHg in the water column. Considering different hydrodynamic/seasonal conditions, water samples were collected along the estuary to evaluate Hg and MeHg distribution and partitioning behaviour between solid and aqueous phases. The complementary effect of the river discharge and tidal currents contributed to the prevalence of the dissolved (4.02 ± 1.33 ng L−1) or particulate (8.37 ± 4.20 ng L−1) Hg under different conditions of discharge in summer and autumn, respectively. Conversely, particulate MeHg prevailed when the river flow was low, especially at the estuary mouth (25.8 ± 19.1 pg L−1) and most likely due to the resuspension of fine particles promoted by a stronger tidal current. In comparison with the total Hg concentration, extremely low amounts of dissolved and particulate MeHg were observed, and strong interactions between MeHg and organic carbon highlighted a negligible risk of increased mobility and potential bioaccumulation of MeHg.

Proposed Environmental Risk Management Elements in a Carpathian Valley Basin, within the Roşia Montană European Historical Mining Area.

Non-ferrous metals mining activities have long accompanied people, and began in the study area of South East Europe over 2000 years ago. The environment quality is significantly affected by both historic mining activities and contemporary impacts. All these problems, inducing synergic negative effects on local organism communities, have created a chronic state of pollution. The Corna Valley has one of the oldest historical human impacts in Romania due to the influence of mining. Fish and benthic macroinvertebrates have exhibited significant responses to long term mining effects on lotic systems. The analysis of macroinvertebrate communities, correlated with the lack of fish and some biotope characteristics, indicates that the Corna River presents a variety of categories of ecological status between sectors. The lack of fish reveals the poor ecological conditions. Technical and management solutions are proposed here to diminish the historical environmental problems and to avoid future ecological accidents, especially in an attempt to improve any construction plan concerning a possible new de-cyanidation dam and lake. Fish and benthic macroinvertebrates have exhibited significant responses to long term mining effects on lotic systems. Two management zones were identified, an upper zone which can be used as a reference area and a lower zone, where pollution remedial activities are proposed.

Water resources affected by potentially toxic elements in an area under current and historical mining in northwestern Mexico.

Rio Sonora watershed and its aquifer-located in northwest Mexico-have been influenced by mining operations for 140years, possibly causing emissions of potentially toxic elements (PTE) and affecting health of exposed populations. On the basis of available data from governmental surveys (2014-2017) and recent sampling (2018), this study constructed reliable PTE total concentration database that allowed us to report temporal/spatial variations in surface and groundwater and their associated health risks to the population living in the central part of the Rio Sonora basin. The data clearly showed that a mining spill that took place in 2014 has had an adverse impact on total PTE concentrations in surface water. They also indicated the presence of different PTE point source locations that have continued to cause contamination of surface water at levels of health concern. Data also suggested slight impacts of the spill event on groundwater possibly related to soil neutralizing potential. Two metal groups were detected for surface waters (Pb-Cd-As-Ni-Cr and of Zn-Al-Cr) and groundwaters (Cr-As-Cu-Cd and Zn-Al), which suggest that they have different sources or are being released by different processes. The potential health impacts of PTE concentrations were associated with specific age groups, dates, and areas. The results indicate that in this complex semi-arid rural system, current and historical mining activities, as well as contrasting hydrological conditions, have impacted surface and groundwater quality with important ecological and human health risks.

Flash floods controlling Cu, Pb, As and Hg variations in fluvial sediments of a river impacted by metal mining in the Atacama Desert

Solid mine wastes can be dispersed in river systems by fluvial processes contaminating distant areas. This process has been previously reported in rivers of the Atacama Desert impacted by mining. The removal of waste in these rivers is a complex environmental scenario that involves (i) mining operation plans, (ii) the spatio-temporal variability of precipitation events and, (iii) the sediment-transport pathways within the drainage network. This work presents how flash floods triggered by extreme storm events determine the redistribution and the variation of elemental concentrations of the suspended load fraction in a river with several historical and actual mine wastes situated in the Atacama Desert. The concentrations of Cu, Pb, As and Hg measured in two sampling stations of a mining segment of the Copiapó River after flash floods are lower compared to the concentrations measured in the period before these hydrometeorological events. However, the concentrations of these metals and metalloids increased in areas distant from the mine clusters, where the values observed before the flash floods were lower. The redistributions of the element concentrations can be explained by the occurrence of two major flood events triggered by the extreme storm events of March 2015 and May 2017. The combination of the element concentrations with calculated geo-accumulation indexes shows the potential source of geochemical anomalies for these elements. Cu and Hg anomalies are induced by historical mining activities in the Copiapó River while Pb and As concentrations agree with the regional background. Some Pb and As anomalies would be associated with the geology of the zone where the main cluster of mine exploitations are present. The fact that flash floods result in Cu, Pb, As and Hg concentrations in sediment load more in line with the local background, suggests that this type of hydrometeorological events probably represent the natural processes of geochemical recovery of arid river impacted by metal mining.

Accumulation of trace metals in freshwater macroinvertebrates across metal contamination gradients.

Historical mining activities cause widespread, long-term trace metal contamination of freshwater ecosystems. However, measuring trace metal bioavailability has proven difficult, because it depends on many factors, not least concentrations in water, sediment and habitat. Simple tools are needed to assess bioavailabilities. The use of biomonitors has been widely advocated to provide a realistic measure. To date there have been few attempts to identify ubiquitous patterns of trace metal accumulation within and between freshwater biomonitors at geographical scales relevant to trace metal contamination. Here we address this through a nationwide collection of freshwater biomonitors (species of Gammarus, Leuctra, Baetis, Rhyacophila, Hydropsyche) from 99 English and Welsh stream sites spanning a gradient of high to low trace metal loading. The study tested for inter-biomonitor variation in trace metal body burden, and for congruence amongst accumulations of trace metals within taxa and between taxa across the gradient. In general, significant differences in trace metal body burden occurred between taxa: Gammarus sp. was the most different compared with insect biomonitors. Bivariate relationships between trace metals within biomonitors reflected trace metal profiles in the environment. Strong correlations between some trace metals suggested accumulation was also influenced by physiological pathways. Bivariate relationships between insect biomonitors for body burdens of As, Cu, Mn and Pb were highly consistent. Our data show that irrespective of taxonomic or ecological differences, there is a commonality of response amongst insect taxa, indicating one or more could provide consistent measures of trace metal bioavailability.

USING DEEP LEARNING AND HOUGH TRANSFORMATIONS TO INFER MINERALISED VEINS FROM LIDAR DATA OVER HISTORIC MINING AREAS

Abstract. This paper presents a novel technique to improve geological understanding in regions of historic mining activity. This is achieved through inferring the orientations of geological structures from the imprints left on the landscape by past mining activities. Open source high resolution LiDAR datasets are used to fine-tune a deep convolutional neural network designed initially for Lunar LiDAR crater identification. By using a transfer learning approach between these two very similar domains, high accuracy predictions of pit locations can be generated in the form of a raster mask of pit location probabilities. Taking the raster of the predicted pit location centres as an input, a Hough transformation is used to fit lines through the centres of the detected pits. The results demonstrate that these lines follow the patterns of known mineralised veins in the area, alongside highlighting veins which are below the scale of the published geological maps.

Effects of Long-Term exposure to Heavy Metals upon Rhizosphere Bacteria from Baia Mare Area (Maramureş County, Romania)

The aim of this study was to investigate the extent of heavy metal (HM) pollution and its effect on microorganisms from rhizosphere soil in Baia Mare area (Maramureş County, Romania). Two sites with different contamination degrees were included in the study: one with a long history of mining activities and one within a drinking water safeguard zone. Rhizosphere soil samples were characterized with respect to physico-chemical parameters and the Cd, Cu, Pb and Zn contents. Native bacteria were investigated for HM tolerance and biofilm formation under toxic exposure by the microdilution assay. The most resistant strains were identified and the minimum inhibitory concentrations for HMs were determined. Cd, Cu, Pb and Zn exceeded the intervention threshold in Bozânta tailings site, while Pb content exceeded the intervention level within the area of the drinking water treatment plant. Cd showed a very high potential ecological risk in Bozânta area. The long-term exposure to HMs contributed to the selection of HM-tolerant and weakly adherent strains. Biofouling was significantly reduced under the influence of copper ions. Arthrobacter, Rhodococcus and Acidovorax strains with exceptional resistant profiles were isolated from the tailings site, indicating the important role of native microorganisms in rhizosphere ecosystems of contaminated sites.

Improving the regulatory health risk assessment of mercury-contaminated sites

An alternative risk assessment strategy for mercury (Hg)–contaminated sites is proposed with bioaccessible fractions and soil Hg vapor (SHgV) concentrations. The new strategy avoids the conservatism of assessment rely on soil total Hg (THg) content and inaccuracy caused by predicted SHgV concentration. The exposure risk to Hg-contaminated soil associated with historical mining activities in Guizhou, China, was evaluated using the proposed strategy. The experimental results revealed that the average bioaccessibility in gastric, intestinal and lung phases was 10.39 % (2.09 % ∼ 35.28 %), 1.28 % (0.23 % ∼ 4.3 %), and 11.27 % (5.04 % ∼ 20.71 %), respectively. Via the proposed strategy, the Hg risk for the oral ingestion pathway, represented as the hazard quotient (HQ), decreased from 1.57 to an acceptable level of 0.19 (<1). The risk of SHgV inhalation sharply decreased from 1168 to 0.35 while the soil PM10 inhalation pathway did not exhibit significant variations. The dominant exposure pathways turned to oral intake and inhalation of SHgV by the strategy. The results indicated that the proposed assessment strategy can greatly improve the understanding of the exposure risk level at Hg-contaminated sites and provide a reasonable decision basis for decision makers.

Differences in metal tolerance among strains, populations, and species of marine diatoms – Importance of exponential growth for quantification

Strains of microalgae vary in traits between species and populations due to adaptation or stochastic processes. Traits of individual strains may also vary depending on the acclimatization state and external forces, such as abiotic stress. In this study we tested how metal tolerance differs among marine diatoms at three organizational levels: species, populations, and strains. At the species level we compared two pelagic Baltic Sea diatoms (Skeletonema marinoi and Thalassiosira baltica). We found that the between-species differences in tolerance (EC50) to the biologically active metals (Cu, Co, Ni, and Zn) was similar to that within-species. In contrast, the two species differed significantly in tolerance towards the non-essential metals, Ag (three-fold higher in T. baltica), Pb and Cd (two and three-fold higher in S. marinoi). At the population level, we found evidence that increased tolerance against Cu and Co (17 and 41 % higher EC50 on average, respectively) had evolved in a S. marinoi population subjected to historical mining activity. On a strain level we demonstrate how the growth phase of cultures (i.e., cellular densities above exponential growth) modulated dose-response relationships to Ag, Cd, Co, Cu, and Zn. Specifically, the EC50's were reduced by 10–60 % in non-exponentially growing S. marinoi (strain RO5AC), depending on metal. For the essential metals these differences were often larger than the average differences between the two species and populations. Consequently, without careful experimental design, interactions between nutrient limitation and metal stress may interfere with detection of small, but evolutionary and ecologically important, differences in tolerance between microalgae. To avoid such artifacts, we outline a semi-continuous cultivation approach that maintains, and empirically tests, that exponential growth is achieved. We argue that such an approach is essential to enable comparison of population or strain differences in tolerance using dose-response tests on cultures of microalgae.

RADIATION SITUATION IN THE TERRITORIES AFFECTED BY MINING ACTIVITIES IN STEPNOGORSK AREAS, REPUBLIC OF KAZAKHSTAN: PILOT STUDY.

The Republic of Kazakhstan has a long history of mining activities, viz., gold and uranium. Mining activities represent sources of potential naturally occurring radionuclides contamination of the environment and human health of population. The aim of this study was to investigate the radiation situation of industrially modified environment in Stepnogorsk areas of Kazakhstan to understand the sources of contamination. Quite high values of ambient equivalent dose rates in air ~2.87μSvh-1 were found in the Aqsu gold-mining site. The radon equivalent equilibrium volume activity (indoor) were in the range of 313-858Bqm-3 in the study area buildings. The high values of activity concentration of natural radionuclides found in Aqsu soil samples were 226Ra-4060, 232Th-1170 and 40K-4080Bqkg-1, respectively. However, our comprehensive surveys implied that the increased natural radiation background caused by the radionuclide transport from the tailing area did not have an impact, while evaluation of its potential radiation risks and remediation of the territories of the former gold mining should be needed.