INTRODUCTION: Our objective was to determine the utility of anti-Müllerian hormone levels in predicting clinical pregnancy in women undergoing intrauterine insemination. METHODS: Retrospective chart review of women undergoing natural and stimulated intrauterine insemination cycles at a single institution with serum anti-Müllerian hormone levels measured within 1 year of first treatment. All patients had either achieved clinical pregnancy in three or fewer intrauterine insemination cycles or had completed three intrauterine insemination cycles without pregnancy. A receiver operating curve (ROC) was generated to determine the ability of anti-Müllerian hormone to predict clinical pregnancy with intrauterine insemination. Characteristics of those with and without pregnancy were compared using Mann-Whitney U, χ2, and Fisher's exact tests. Correlations between anti-Müllerian hormone and other demographic parameters were analyzed by Spearman correlation. RESULTS: A total of 215 women were identified; 102 (47%) achieved pregnancy in three or fewer intrauterine insemination cycles. The area under the ROC was 0.638 in predicting clinical pregnancy within three consecutive intrauterine insemination cycles using anti-Müllerian hormone. Pregnant patients were more likely to have a higher anti-Müllerian hormone level (2.77 compared with 1.54 ng/mL, P=.0005), higher body mass index (BMI, calculated as weight (kg)/[height (m)]2) (26.2 compared with 24, P=.006), higher antral follicle count (greater than 8) (P=.005), and a history of live birth (39% compared with 24%, P=.018). Anti-Müllerian hormone level correlated positively with BMI and antral follicle count and inversely with age and day 3 follicle-stimulating hormone level. The area under the ROC was 0.636 for predicting clinical pregnancy using antral follicle count. CONCLUSION: Although serum anti-Müllerian hormone level appears significantly higher in women achieving clinical pregnancy, anti-Müllerian hormone is a poor predictor of clinical pregnancy outcome with intrauterine insemination.