The stable carbon isotope composition of total organic matter (δ13Corg) has been utilized in aeolian sediments, serving as an indicator for reconstructing terrestrial paleoenvironments. The Qinghai Lake (QHL) Basin is a climate-sensitive region of significant importance in paleoclimatic reconstruction. However, the reconstructed climatic variations based on δ13Corg in aeolian sediments in the QHL Basin in the northeastern Qinghai-Tibet Plateau (QTP) are lacking, and their paleoclimatic significance remains poorly understood. By conducting δ13Corg measurements on the Niaodao (ND) aeolian profile near QHL, we reconstructed the paleoclimate changes of 11 ka–present. The variation range of the δ13Corg values in the ND profile indicated the terrestrial ecosystems were not the sole contributor to lacustrine organic matter. The δ13Corg values are an indicator of historical temperature changes in the study area, exhibiting similar trends with the reconstruction of Chinese summer temperatures, East Asian air temperature, global temperature, and Northern Hemisphere summer insolation at 37° N. The temperature increased with high frequency and amplitude oscillations, with strong aeolian activity and low total organic carbon accumulation during the Early Holocene. The temperature was maintained at a high and stable level, with the weakest aeolian activity and intensified pedogenesis during the Middle Holocene. The temperature decreased at a high rate, with renewed aeolian activity and weak pedogenesis during the Late Holocene.