Histopathological Profile Research Articles

Partial spontaneous regression of choroidal melanoma: A case image with histopathology and gene expression profiling

Partial spontaneous regression of choroidal melanoma: A case image with histopathology and gene expression profiling

A comparison of central compartment atopic disease and lateral dominant nasal polyps.

The characteristics and surgical outcomes of central compartment atopic disease (CCAD) vary by region and race. Therefore, we aimed to identify the risk factors, symptom severity, and prognosis of CCAD in the Asian population. This case-control study recruited patients diagnosed with chronic rhinosinusitis with nasal polyps who underwent functional endoscopic sinus surgery (FESS) at a tertiary hospital in Taiwan. Patients were classified into CCAD and lateral-dominant nasal polyp (LDNP) groups based on endoscopic and computed tomography imaging findings. The demographic data, symptom severity scores, and surgical outcomes of the 2 groups were analyzed. Our study included 442 patients (CCAD group: n = 51; LDNP group: n = 391). We found that CCAD was strongly related to both asthma (9.8% vs 3.5%, p = 0.04) and allergic rhinitis symptoms (43.3% vs 26.6%, p = 0.01). Higher eosinophil counts were detected in blood serum (5.8% vs 2.8%, p < 0.01) and histopathologic profiles (57.0 vs 17.3, p < 0.01) among patients with CCAD. Improvements in 22-item Sino-Nasal Outcome Test (SNOT-22) score and mucociliary clearance time (MCT) after surgical intervention revealed that the CCAD group had a better response to FESS (SNOT-22 score: -31.82 vs -22.66, p < 0.01; MCT: -233.06 vs -191.93 seconds, p = 0.03). The revision FESS rate was not different between the 2 groups. Polyps originating from the central compartment were found to be related to asthma and allergic rhinitis in Taiwanese patients. A higher eosinophil count was suggested in both serum and local nasal tissue from patients with CCAD. FESS serves as an effective treatment for symptom relief in patients with CCAD.

Lung histopathological profile of male albino Wistar rats exposed to tobacco smoke administered ethanolic extract of red spinach

Background: Exposure to tobacco smoke, which contains free radicals, can cause oxidative stress in the lung. Oxidative stress can trigger lung inflammation due to dilation of the alveolar lumen, alveolar wall thickening, and inflammatory cellular infiltration. Red spinach (Alternanthera amoena Voss.) leaves have antioxidant activity in vitro, with IC50 = 4.32 μg/mL. Objective: To assess the antioxidant activity of red spinach in male albino Wistar rats. Methods: The rats were divided into five test groups of five rats each: the negative control (1% Na-CMC suspension), the positive control (vitamin C suspension), and three experimental groups receiving red spinach leaves at a dose of 200 mg/Kg BW, 400 mg/Kg BW, and 600 mg/Kg BW, respectively. All the rats were exposed to tobacco smoke for 14 days using three cigarettes in a smoking chamber. The right lung was taken for histopathology. Results: The results showed that doses of 200 mg/Kg BW, 400 mg/Kg BW, and 600 mg/Kg BW could reduce the level of lung damage, i.e. the enlarged alveolar lumen, thickened alveolar wall, and inflammatory cellular infiltration. Conclusion: The dose of 600 mg/Kg BW was effective in reducing the level of lung damage compared to the negative control.

Juvenile and adult xanthogranuloma: A 30-year single-center experience and review of the disorder and its relationship to other histiocytoses

BackgroundJuvenile xanthogranuloma (JXG) is the most common type of non-Langerhans cell histiocytosis whose cell of origin, etiology and pathogenesis are not fully understood. We aimed to provide an update on histopathologic and immunophenotypic profile of this well-characterized entity whose relationship to the other histiocytoses has received renewed attention in light of recent molecular genetic studies. Materials and methodsA retrospective review of all the cases with the pathologic diagnosis of “xanthogranuloma” was performed on our archives from 1989 to 2019. ResultsA total of 525 patients with 547 lesions diagnosed as JXG were identified with the median age of 4.5 years, a male predominance (M:F ratio 1.3:1) and a predilection for the head and neck region (40.8%). Cutaneous lesions comprised 76.8% cases and another 15.7% presented within soft tissues. The most common non-soft tissue, extracutaneous lesions included the brain (2.6%), and lungs (1.8%). Three basic histopathologic patterns were identified: early classic (EJXG) (14.2%), classic (CJXG) (45.3%), and transitional JXG (TJXG) (40.5%). Multinucleated giant cells, either Touton or non-Touton, were most frequently present in CJXG followed by TJXG. Mitosis was rare (<1/10 high-power field) among different patterns. There was an association among the patterns and lymphocytic infiltrates (P = 0.036), and presence of Touton or non-Touton giant cells (P < 0.001 for both) but not for mitotic count (P = 0.105) or eosinophilic infiltrates (P = 0.465). Additionally, there was a correlation between age groups and presence of non-Touton giant cells (P = 0.012) but not for Touton cells (P = 0.127). We have demonstrated that immunophenotypic expression of the lesion was not associated with age at diagnosis nor morphologic pattern: factor XIIIa 192/204 (94.1%), CD11c 75/77 (97.4%), CD4 82/84 (97.6%), CD68 200/201 (99.5%), CD163 15/15 (100%), CD1a 1/110 (0.9%), S-100 48/152 (31.6%), CD31 15/21 (71.4%), and vimentin 104/105 (99.0%). ConclusionWe have documented in a substantial series of cases of JXG that there is a correlation between one of the three basic histopathologic patterns with age at diagnosis, but with a consistent immunophenotype among the three patterns. Considering sensitivity and specificity rates, we suggest that a combination of CD11c, CD4, CD1a and either CD163 (preferred) or CD68 stains provides more specific diagnostic yield in the differentiation of JXG from other histiocytic disorders. JXG is also discussed in terms of its relationship and distinction from other similar histiocytic disorders in the context of MAPK/ERK pathway mutations.

A Study of Multivariate Modalities of Therapy in Gall Bladder Malignancies with Different Clinical, Radiological and Histo-Pathological Profiles at a Low Resource Indian Cancer Centre

Introduction: The effectiveness of adjuvant radiation therapy (RT) in the treatment of GBC has not yet been established. The effectiveness of adjuvant radiation therapy (RT) in the treatment of GBC has not yet been established. Following surgical resection, postoperative external beam RT can diminish local relapse; though effect on global survival has not been confirmed due to lack of good quality clinical trials. Thus the aim of the study was to assess the efficacy of the multivariate modality of therapy in Gallbladder malignancies. Materials and method: The present prospective comparative study of multivariate modality of therapy in Gallbladder malignancies was carried out in a low resource North Indian cancer center from. Only those patients were selected who were histologically confirmed of adenocarconima of gall bladder, squamous cell carcinoma and oat cell carcinoma. Staging was done according to TNM classification no concurrent medical illness, Karnofsky performances status of patient &gt;70. Statistical analyses were performed using SPSS version 23.0 (SPSS Inc., Chicago, IL, USA). A value of p&lt;0.05 was considered statistically significant. Result: Majority (29.3%) of the cases were aged 61-70 years just followed by the age group 41-50 years, females were in majority (73.2%). Higher stage had significantly higher risk of partial or no response (p&lt;0.001). An increasing trend of toxicities was observed with increasing dose. Significant association was found between toxicities and treatment response at dose 2000 cGy (p&lt;0.001) and 4500 cGy (p&lt;0.001). The Cox regression analysis showed higher hazard ratio for stage IV (HR=2.33 95% CI : 0.31-17.66) followed by stage II/III (HR=1.67 95% CI : 0.17-16.02). Conclusion: Healthcare professionals should discuss the indeterminate benefit and possible risks of adjuvant therapy when counseling their patients and reassure enrollment in clinical trials of novel regimens. The curative potential of current adjuvant therapy in gallbladder cancer is questionable, justifying placebo-controlled investigation of novel chemotherapy combinations or alternative approaches. To confirm the efficacy of adjuvant therapy, a major randomized controlled trial is required with large sample size. The improvement in radiation delivery with intensity modulated radiotherapy (IMRT), image guided radiotherapy has further paved the way for exploration of adjuvant radiation for GBC.

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder.

As an economically important flatfish in Asia, Japanese flounder is threatened by continuously rising temperatures due to global warming. To understand the molecular responses of this species to temperature stress, adult Japanese flounder individuals were treated with two kinds of heat stress—a gradual temperature rise (GTR) and an abrupt temperature rise (ATR)—in aquaria under experimental conditions. Changes in histopathology, programmed cell death levels and the oxidative stress status of gills were investigated. Histopathology showed that the damage caused by ATR stress was more serious. TUNEL signals confirmed this result, showing more programmed cell death in the ATR group. In addition, reactive oxygen species (ROS) levels and the 8-O-hDG contents of both the GTR and ATR groups increased significantly, and the total superoxide dismutase (T-SOD) activities and total antioxidant capacity (T-AOC) levels decreased in the two stressed groups, which showed damage to antioxidant systems. Meanwhile, RNA-seq was utilized to illustrate the molecular mechanisms underyling gill damage. Compared to the control group of 18 °C, 507 differentially expressed genes (DEGs) were screened in the GTR group; 341 were up-regulated and 166 were down-regulated, and pathway enrichment analysis indicated that they were involved in regulation and adaptation, including chaperone and folding catalyst pathways, the mitogen-activated protein kinase signaling (MAPK) pathway and DNA replication protein pathways. After ATR stress, 1070 DEGs were identified, 627 were up-regulated and 423 were down-regulated, and most DEGs were involved in chaperone and folding catalyst and DNA-related pathways, such as DNA replication proteins and nucleotide excision repair. The annotation of DEGs showed the great importance of heat shock proteins (HSPs) in protecting Japanese flounder from heat stress injury; 12 hsp genes were found after GTR, while 5 hsp genes were found after ATR. In summary, our study records gill dysfunction after heat stress, with different response patterns observed in the two experimental designs; chaperones were activated to defend heat stress after GTR, while replication was almost abandoned due to the severe damage consequent on ATR stress.

Evaluation of the impact of pre-operative stereotactic radiotherapy on the acute changes in histopathologic and immune marker profiles of brain metastases

The unique acute effects of the large fractional doses that characterize stereotactic radiosurgery (SRS) or radiotherapy (SRT), specifically in terms of antitumor immune cellular processes, vascular damage, tumor necrosis, and apoptosis on brain metastasis have yet to be empirically demonstrated. The objective of this study is to provide the first in-human evaluation of the acute biological effects of SRS/SRT in resected brain metastasis. Tumor samples from patients who underwent dose-escalated preoperative SRT followed by resection with available non-irradiated primary tumor tissues were retrieved from our institutional biorepository. All primary tumors and irradiated metastases were evaluated for the following parameters: tumor necrosis, T-cells, natural killer cells, vessel density, vascular endothelial growth factor, and apoptotic factors. Twenty-two patients with irradiated and resected brain metastases and paired non-irradiated primary tumor samples met inclusion criteria. Patients underwent a median preoperative SRT dose of 18 Gy (Range: 15–20 Gy) in 1 fraction, with 3 patients receiving 27–30 Gy in 3–5 fractions, followed by resection within median interval of 67.8 h (R: 18.25–160.61 h). The rate of necrosis was significantly higher in irradiated brain metastases than non-irradiated primary tumors (p < 0.001). Decreases in all immunomodulatory cell populations were found in irradiated metastases compared to primary tumors: CD3 + (p = 0.003), CD4 + (p = 0.01), and CD8 + (p = 0.01). Pre-operative SRT is associated with acute effects such as increased tumor necrosis and differences in expression of immunomodulatory factors, an effect that does not appear to be time dependent, within the limited intervals explored within the context of this analysis.

Salvia hispanica L. seeds extract alleviate encephalopathy in streptozotocin-induced diabetes in rats: role of oxidative stress, neurotransmitters, DNA and histological indices

Context Diabetes mellitus (DM) is a metabolic disorder and may lead to cognitive dysfunctions. Objective The aim of this work is to evaluate the potency of Salvia hispanica L. seeds (S. hispanica L.) (chia seeds) petroleum ether extract in attenuating brain complications associated with streptozotocin (STZ) induced diabetes in rats. Materials and methods Phytochemical composition of the seeds extract, macro and micro elements, vitamins, protein, carbohydrate and caloric values were estimated. Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg body weight (b.wt)). Glibenclamide as a reference drug was also evaluated. The biochemical evaluation was done by measuring levels of glucose, insulin, α- amylase, glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), dopamine (DA), serotonin (5-HD), noradrenaline (NE), acetylcholinesterase (AchE), tumour necrosis factor-α (TNF-α), DNA fragmentation pattern and the histopathological profile of the brain hippocampus region. Results Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of twenty-five fatty acid esters and twenty-two compounds. Column chromatography led to the isolation of nine compounds. Treatment with the seeds extract revealed improvement of the measured parameters with variable degrees. Conclusion Chia seeds extract succeeded to attenuate the neurodegeneration in diabetic rats. Thereafter, it had a therapeutic effect and could be potentially used as a new dietary supplement against diabetic encephalopathy.

Histopathological profile of cervical biopsies in northern Malawi: a retrospective cross-sectional study

ObjectivesAccording to the WHO (2014), cervical cancer is the second most common cancer in women globally. More than 85% of the global cervical cancer morbidity and mortality occur in low-income...

Pregnancy-associated breast cancer: the influence of gestational age.

Whether pregnancy-associated breast cancer (PABC) arise before or during pregnancy and whether this histopathology is affected by gestational age are currently unclear. The present study assesses the influence of gestational age and lactation on the histopathologic profile of PABC. We identified 744 patients with PABC (defined as breast cancer during pregnancy or 6 months following delivery). Histopathologic features were compared between pregnant and postpartum patients. We found that age at diagnosis was 34.2 years, and a majority of cancers were diagnosed during pregnancy (71.3%). Within pregnant patients, tumors were significantly more often estrogen receptor (ER)-negative in second and third trimesters (57.4%), as compared to first trimesters (41.9%) (P = 0.036). Similarly, a progesterone receptor (PR)-negative status was reported significantly less often within first trimesters (38.0%) compared to second and third trimesters (57.1%) (P = 0.032). For human EGF receptor 2 status, no significant differences were observed between gestational trimesters or lactating vs non-lactating patients. In postpartum patients, grade III tumors were found in over 80%, with high percentages of ER-negative tumors reaching 63% in those lactating vs 49% in non-lactating patients. This study demonstrates the varying histopathologic profile of PABC by gestational age and lactation status. Second- and third-trimester cancers display most typically the common ER/PR-negative phenotype, which is commonly reported in the literature. The increased ER-negative status and percentage grade III tumors in lactating vs non-lactating patients also suggest the presence of additional factors further diversifying histology. This indicates the need for clear definitions of PABC and the role of potential subgroups, which may provide a stepping stone for further in-depth research into PABC-carcinogenesis.

Histopathological profile of orbito-ocular cancers at a tertiary hospital in Northern Malawi: a retrospective cross-sectional study

ObjectiveOrbito-ocular cancers are a common cause of morbidity and mortality in our population, yet these cancers have not been adequately described in Malawi. This study sought to describe the burden...

Abstract P3-12-06: Prognosis of pregnancy-associated breast cancer: Inferior outcome in patients diagnosed during second and third gestational trimesters and lactation

Abstract BACKGROUND Pregnancy-associated breast cancer (PABC), although most commonly defined as breast cancer diagnosed during pregnancy or within one year following delivery, knows a variety of definitions, likely related to the diversity of reported clinicopathological features and prognosis. More insight into the different breast cancer subgroups during pregnancy, the time after delivery and the postpartum period is therefore warranted. METHODS Patients with breast cancer diagnosed during pregnancy or within six months after delivery between January 1, 1988 and July 1, 2019, were included. Pregnant patients were subdivided according to gestational trimester, and postpartum patients according to lactational status. To investigate the influence of pregnancy and lactation on the histopathologic profile, these subgroups were compared to non-PABC patients matched for age at diagnosis, year at diagnosis, grade and ER status. Pearson Chi-square tests were used to compare clinicopathologic characteristics, while Kaplan-Meier/logrank/Cox regression methods were used to perform overall survival (OS) analysis. RESULTS Overall, 662 PABC patients were included, of which 73.6% were diagnosed during pregnancy. Median age at diagnosis was 34 years, with a median follow-up of 6.5 years. Overall, PABC patients as a group showed an advanced stage at diagnosis and an inferior OS at 5-years (75.4% vs. 83.2%, p = 0.000) compared to 1,392 non-PABC patients. In subgroup analysis, PABC patients within their first trimester showed a significantly lower tumor size and stage as compared to other trimesters. Patients diagnosed during the first trimester and postpartum non-lactating patients had a relatively good OS (81.3% and 77.9%, respectively) versus patients diagnosed during the second and third trimesters and during lactation (OS 60.0%, 64.9% and 65.6%, respectively, p = 0.003). In multivariate Cox regression, trimester of diagnosis, year of diagnosis, PR status, stage at diagnosis and surgery-or-not were significant contributors to OS. CONCLUSION In this large PABC cohort, uniquely specified by gestational trimester, an inferior outcome was found for patients diagnosed within the second and third gestational trimesters and during postpartum lactation, compared to first trimester and non-lactating postpartum patients. These findings indicate that PABC is clinically a heterogeneous group of breast cancer patients that should be redefined based on trimester of diagnosis and postpartum lactational status. Citation Format: Britt BM Suelmann, Carsten FJ Bakhuis, Carmen van Dooijeweert, Janneke Verloop, Ronald P Zweemer, Sabine C Linn, Elsken van der Wall, Paul J van Diest. Prognosis of pregnancy-associated breast cancer: Inferior outcome in patients diagnosed during second and third gestational trimesters and lactation [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr P3-12-06.

Radiological features of oral and maxillofacial plasmablastic lymphomas.

The purpose of the current study was to report on the clinical presentation and radiological features of 72 cases of oral and maxillofacial plasmablastic lymphomas (PBL). Histologically confirmed cases of PBL were retrospectively reviewed over a 10-year period. Demographic and clinical data were retrieved from the patient's records. Radiological information was obtained from the available radiographic examinations. The prevalence of PBL was 0.6% of diagnosed head and neck lesions. PBL presented at a mean age of 39years, demonstrating a strong male predominance. All patients with a known retroviral disease (RVD) status were HIV-positive. PBL had a maxillary predilection (78%), with posterior regions of both jawbones most commonly affected (76%). Most cases had bony involvement (95%), with poorly demarcated bony borders seen in the majority of cases (93%). PBL had a tendency to cause a loss of cortical integrity (96%), either via cortical erosion or destruction, rather than cortical expansion (2%). Most of the current literature on PBL focuses on demographics, anatomical location, and histopathological profile, with radiological features only reported in isolated case reports. This study is the first of its kind to report on the clinicoradiological appearance of PBL in a large sample. These findings may assist clinicians in the clinical diagnostic work-up of patients, including the acquisition and assessment of appropriate advanced radiographic imaging.

Integrated histopathological, lipidomic, and metabolomic profiles reveal mink is a useful animal model to mimic the pathogenicity of severe COVID-19 patients

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is transmitted on mink farms between minks and humans in many countries. However, the systemic pathological features of SARS-CoV-2-infected minks are mostly unknown. Here, we demonstrated that minks were largely permissive to SARS-CoV-2, characterized by severe and diffuse alveolar damage, and lasted at least 14 days post inoculation (dpi). We first reported that infected minks displayed multiple organ-system lesions accompanied by an increased inflammatory response and widespread viral distribution in the cardiovascular, hepatobiliary, urinary, endocrine, digestive, and immune systems. The viral protein partially co-localized with activated Mac-2+ macrophages throughout the body. Moreover, we first found that the alterations in lipids and metabolites were correlated with the histological lesions in infected minks, especially at 6 dpi, and were similar to that of patients with severe and fatal COVID-19. Particularly, altered metabolic pathways, abnormal digestion, and absorption of vitamins, lipids, cholesterol, steroids, amino acids, and proteins, consistent with hepatic dysfunction, highlight metabolic and immune dysregulation. Enriched kynurenine in infected minks contributed to significant activation of the kynurenine pathway and was related to macrophage activation. Melatonin, which has significant anti-inflammatory and immunomodulating effects, was significantly downregulated at 6 dpi and displayed potential as a targeted medicine. Our data first illustrate systematic analyses of infected minks to recapitulate those observations in severe and fetal COVID-19 patients, delineating a useful animal model to mimic SARS-CoV-2-induced systematic and severe pathophysiological features and provide a reliable tool for the development of effective and targeted treatment strategies, vaccine research, and potential biomarkers.

Ochratoxin A occurrence, its pathological effects on poultry health and decontamination approaches

Mycotoxins are filamentous fungi which can be seen microscopically and they impart adverse effects on poultry health. The occurrence of mycotoxins in poultry feed imparts adverse effects not only to the birds’ health but also causes huge economic losses by affecting feed conversion ratio, production of meat and eggs,and also responsible for histopathological changes in lymphoid organs of chicken. More than 300 different species of mycotoxins have been reported from which the ochratoxin and aflatoxins are the most harmful for poultry industry. Among ochratoxins, especially the Ochratoxin A (OTA) is produced from Aspergillus Ochraceus and and Penicillium verrucosum species as storage fungi inside stored drains and feed ingredients. The ochratoxicity is a dose-dependent factor and hits the immune system of birds besides decreased feed intake and decreased body weight. Higher doses of OTA toxicity resulted in deterioration of egg shell quality and hatchability in layers and breeders. Histopathological profile of ochratoxicity affected birds showed severe to moderate changes in the kidney, bursa of Fabricius, liver, thymus, spleen, heart, and lungs depending on dosage. In this review article, an attempt has been made to concise the OTA associated alterations in growth pattern, production index, biochemical changes and histopathological profile of the poultry. The current approaches and agents (Vitamin-E, Silymarin), which are being used to reduce the effects of OTA toxicity in birds, have also been discussed.

A Multidisciplinary Approach Evaluating Soybean Meal-Induced Enteritis in Rainbow Trout Oncorhynchus mykiss

This study evaluated a diverse range of markers of feeding stress to obtain a more precise assessment of the welfare of rainbow trout in relation to inadequate husbandry conditions. A feeding stress model based on dietary soybean meal was employed to identify suitable minimally invasive “classical” stress markers, together with molecular signatures. In a 56-day feeding experiment, rainbow trout were fed diets containing different levels of soybean meal. The impact of these different soybean meal diets on rainbow trout was assessed by water quality analyses, clinical health observations, classic growth and performance parameters, gut histopathology, blood-parameter measurements and multigene-expression profiling in RNA from whole blood. Soybean meal-induced enteritis was manifested phenotypically by an inflammatory reaction in the posterior section of the intestine and by diarrhoea in some trout. These inflammatory changes were associated with decreased supranuclear vacuolation. The haematocrit values and the levels of plasma cortisol and circulating lymphocytes in the blood were increased in trout that had consumed high amounts of SBM. Notably, the increased haematocrit depended significantly on the bodyweight of the individual trout. The transcript levels of certain genes (e.g., MAP3K1, LYG, NOD1, STAT1 and HSP90AB) emerged as potentially useful indicators in the blood of rainbow trout providing valuable information about inadequate nutrition. The expression-profiling findings provide a basis for improved, minimally invasive monitoring of feeding regimens in trout farming and may stimulate the development of practical detection devices for innovative aquaculture operations.

Analysis of Histopathological Findings of Lung Carcinoma in Czech Black Coal Miners in Association with Coal Workers' Pneumoconiosis.

Coal miners with coal workers’ pneumoconiosis (CWP, J60 according to ICD-10) were previously found to have a significantly higher risk of lung carcinoma compared to the general male population. The presented study aimed to analyze the (i) incidence of lung carcinoma in miners, (ii) histopathological findings in cohorts with and without CWP, and (iii) effect of smoking cessation on the histopathological profile. Analyzed cohorts consisted of miners with (n = 3476) and without (n = 6687) CWP. Data on personal and working history obtained from the medical records were combined with information on lung cancer from the Czech Oncological Register and histopathological findings. Statistical analysis was performed using non-parametric tests and the incidence risk ratio at the significance level of 5%. In 1992–2015, 180 miners (2.7%) without CWP and 169 (4.9%) with CWP, respectively, were diagnosed with lung carcinoma. The risk of lung cancer in miners with CWP was 1.82 (95% CI: 1.48–2.25) times higher than in those without CWP. Squamous cell carcinoma (37%) was the most common histopathological type, followed by adenocarcinoma (22%) and small cell carcinoma (21%). A statistically significant difference between the cohorts (p = 0.003) was found in the histopathological subtypes, with the incidence of small cell carcinoma being 2 times higher in miners without CWP than in those with CWP. Only a few individuals with lung carcinoma were non-smokers. The incidence of small cell carcinoma, which is strongly associated with smoking, is significantly higher in miners without CWP. Smoking constitutes the most important risk factor for developing lung carcinoma even in that cohort. However, CWP remains a very important risk factor.

Study on Effects of Medroxyprogesterone Acetate on Serological Parameters, Ultrasonographic and Histopathological Profile of Uterine Endometrial Morphology in Female Rabbits

Background: Rabbits are small mammals from the family Leporidae. Rapid multiplication profile of the rabbits often necessitates contraceptive controls in which ovario-hysterectomy is the only recommended method worldwide but still rabbit owners avoid surgical manipulation and opt for the contraceptive injections but contraceptive injections are also known to cause undesired effects in different animal species. The present study was conducted to evaluate effects of Medroxyprogesterone acetate on liver enzymes, lipid profile, blood glucose and uterine endometrial morphology. Methods: The study was conducted on 20 breeding female rabbits at Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan. The study animals were divided into two groups, A and B having 10 rabbits each. Group A was treated with single dose of Medroxyprogesterone acetate (10 mg/kg IM). While group B was kept as control and was given a single dose of 0.9% normal saline placebo injection. Pre and post treatment serum sampling was performed on days 0, 7, 14, 21, 28, 35, 42 and histopathological evaluation of reproductive tract (mainly uterus) was conducted at the end of study. Result: Serological parameters i.e. liver profile, lipid profile and blood glucose level (random) were significantly increased after treatment along with congestion, engorgement of blood vessels, degeneration, hyperplasia of uterine epithelium and uterine glands in Treatment group in contrast to control group. On the basis of this study it was concluded that Medroxyprogesterone acetate poses undesirable effect on the uterine endometrium and different serological parameters of body like liver enzymes (total bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase), blood glucose, lipid profile (cholesterol, high density lipoprotein, low density lipoprotein, triglyceride).

Histopathological Study and Pattern of Lymph Node Involvement in Carcinoma Esophagus: A Tertiary Care Centre Experience from Northern India

Background: Esophageal cancer remains one of the deadliest cancers with an overall 5-year survivalrate estimated to be less than 18%. Squamous cell carcinoma of the esophagus is the predominanthistology in the cervical esophagus and upper and middle thirds of the thoracic esophagus, whereasadenocarcinoma predominates in the distal esophagus. Lymph node involvement and the number oflymph node metastases are important prognostic factorsMaterials and Methods: 30 patients of mid and lower thoracic esophageal carcinoma treated between2008 to 2014 were studied retrospectively. Histopathological profiles were analysed mainly in terms ofpattern of lymph node involvement.Results: 26 cases were squamous cell carcinoma and 3 were adenocarcinoma while 1 case waspathological complete response after neoadjuvant treatment. 22/30 were moderately differentiated.24/30 had lymph node involvement where T3N1 was the commonest stage as 50% had achieved thisstage. Mediastinal group of lymph nodes were involved in 23 cases upper abdominal lymph nodeswere involved only in 1 case while cervical lymph nodes were not involved in any case. Amongmediastinal group paraesophageal, paratracheal lymph node involvement was the commonest followedby subcarinal and recurrent laryngeal group of lymph nodes.Conclusion: Squamous cell carcinoma is still the commonest variety prevailing in this region. Majorityof the cases present in advanced stage with lymph node involvement. Mediastinal group of lymph nodesare involved in majority of the cases hence precise clearance of this group is of utmost importance toprevent local recurrence and prolonged survival.

Case studies on clinico-pathological aspects of concurrent occurrence of mammary tumors and pyometra in female dogs

The present study describes the clinico-pathological aspects of concurrent occurrence of mammary tumors and pyometra in female dogs. Three bitches with growth in mammary gland are brought to the Veterinary Clinical Complex, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu with the history abdominal distension, vomiting, polyuria, polydipsia and mucopurulent vaginal discharge. On clinical examinations animals revealed distended abdomen with pain on palpation. Haematological parameters revealed anaemia. Serum biochemical parameters showed decreased protein and electrolyte levels and increased blood urea nitrogen and creatinine values. Serum hormones such as estradiol and progesterone levels were increased. Radiography revealed moderately distended uterus. The mammary masses and infected uterus from affected bitches were removed by aseptic surgical procedures under general anaesthesia. Cytology of mammary masses showed neoplastic cells characterised by anisocytosis, anisokaryosis and hyperchromasia. Grossly, the infected uterus was edematous, turgid and the lumen was filled with about 200 ml of yellowish creamy pus. Histopathology and immunohistochemistry profile of mammary masses confirmed the cases as mixed mammary carcinoma. Clinical profile and gross pathology of uterus revealed pyometra. Hence, the pathological features of concurrent occurrence of mixed mammary tumor and pyometra in bitches were reported.