Histomorphological Changes Research Articles

Exploring the efficacy of a continuous form of the histologic grade in prostate cancer prognosis prediction.

e17105 Background: Adenocarcinoma of the prostate is the second most common type of cancer among men worldwide. The Gleason grading system remains the gold standard for evaluating the prognosis of prostate cancer by assessing the cancer morphology. However, the current discretized Gleason pattern regime limits the depiction of fine-grained histomorphological changes. We evaluated the efficacy of the algorithm-generated continuous histologic grade value in prostate cancer prognosis prediction. Methods: Whole-slide images of H&E prostatectomy tissue, along with the follow-up data including new tumor event (NTE) and biochemical recurrence (BCR), were obtained for the cases diagnosed as prostate cancer during 2000-2013 from The Cancer Genome Atlas (TCGA) database. Cases with missing data were excluded, resulting in a total of 308 and 397 cases being used for the analysis of NTE and BCR, respectively. Our study utilized a deep learning-based algorithm that performs Gleason scoring as follows. First, it computes per-pixel likelihood values for each of the 4 classes: benign, Gleason 3, 4, and 5. Then, the per-pixel class with the highest likelihood value and the slide-wise ISUP grade group (GG-AI) is successively determined. We tweaked the algorithm to aggregate the likelihood-weighted Gleason grade assigned to each pixel into a continuous form of the histologic grade (c-HG). We compared the prognostic performance of c-HG with that of the original ISUP grade group in TCGA (GG) and that of algorithm-generated GG-AI in predicting the risk of NTE as well as BCR. The Cox regression analysis was conducted, setting the cases from one of three donating institutions (EJ, HC, KK) as the target, fitting the Cox model with the cases from the other institutions, and evaluating the fitted model on the target. Results: The median follow-up duration in months was 32 for NTE and 29 for BCR, respectively. The number of cases with NTE was 51, while the one with BCR was 82. The table presents the c-index values obtained from each institution for GG, GG-AI, and c-HG. Note that the number of cases in each institution is different for NTE and BCR, due to the different number of removed cases with missing data. c-HG showed the best average performance in both NTE and BCR risk predictions. It is also notable that c-HG showed a stable performance for varying institutions. Conclusions: We proposed an algorithm-based method of representing histologic grade as a continuous value, which may give better predictions in disease progression for prostate adenocarcinoma. [Table: see text]

Effect of heat-reinforcing needling on the inflammation and necrotizing apoptosis of synovial cells in synovial tissues of knee joint in rabbits with cold syndrome rheumatoid arthritis

To observe the effect of heat-reinforcing needling (HRN) on inflammatory factors and necrotizing apoptosis of synovial cells in synovial tissues of knee joint in rabbits with cold syndrome rheumatoid arthritis (RA), so as to explore its underlying mechanisms in treating RA. By using the random number table method, 40 New Zealand rabbits were randomly divided into normal, model, antagonist(AG), twist-reinforceing needling (TRN) and HRN groups, with 8 rabbits in each group. The model of cold syndrome RA was established by ovalbumin induction combined with Freund's complete adjuvant injection and cryogenic freezing method. In the AG group, the antagonist TAK-632 (25 mg/kg) was administered intragastrically, once every 2 days, for a total of 7 times. Rabbits of TRN and HRN groups were treated with corresponding acupuncture techniques on bilateral "Zusanli" (ST36) for 30 min, once a day for 14 days. After intervention, the changes of knee skin temperature and circumference were measured. Color Doppler ultrasound was used to observe the joint cavity effusion, synovial thickness and internal blood flow signal. The histomorphological changes of synovial tissues were observed after HE staining. ELISA was used to detect the contents of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in serum. Transmission electron microscope was used to observe the ultrastructure, necrosis and apoptosis of synovial cells. Western blot was used to detect the protein expressions of receptor-interacting protein kinase1 (RIPK1), RIPK3, mixed lineage kinase domain-like protein (MLKL), and phosphorylation （p）-MLKL in synovial tissues. Compared with the normal group, the synovial was diffusely hyperplasia, joint cavity effusion and abnormal blood flow signal were obvious, inflammatory cells were clustered, arranged closely and disordered in the model group. The findings of transmission electron microscopy showed disruption of cell membrane integrity, swollen or ruptured mitochondria, obviously ruptured nucleus, condensed and pyknotic chromatin and nucleolus in the model group. Also, the skin temperature of the knee joint was significantly decreased (P<0.01), while the circumference of the knee joint, the contents of TNF-α, IL-1β and IL-6 in serum, the protein expressions of RIPK1, RIPK3, p-MLKL and MLKL in synovial tissues were significantly increased (P<0.01) in the model group. Compared with the model group, synovial tissue hyperplasia, joint cavity effusion, abnormal blood flow signals, synovial cell proliferation, inflammatory cell infiltration, disruption of cell membrane integrity, cell swelling, cell rupture, and nuclear pyknosis were reduced to different degrees in the AG, TRN and HRN groups. Additionally, the skin temperature of the knee joint was increased (P<0.01, P<0.05), while the circumference of the knee joint, the contents of TNF-α, IL-1β and IL-6 in serum, the expressions of RIPK1, RIPK3, p-MLKL and MLKL in synovial tissues were decreased (P<0.01, P<0.05) in the AG, TRN and HRN groups. The effects of HRN and AG were notably superior to that of TRN in up-regulating skin temperature of the knee joint, and down-regulating the circumference of the knee joint, the contents of TNF-α, IL-1β and IL-6 in serum, the expressions of RIPK1, RIPK3, p-MLKL and MLKL in synovial tissues (P<0.01, P<0.05). HRN can reduce synovial inflammation of knee joint in rabbits with cold syndrome RA, which may be related to its function in inhibiting the necrotizing apoptosis of synovial cells.

Clinicopathological Profile of Ampulla of Vater and Pancreas Malignancies at Cipto Mangunkusumo Hospital in 2015-2019

Background&#x0D; Malignancies of ampulla of vater and pancreas are often difficult to distinguish. Malignancies in ampulla of vater include malignancies in major duodenal papilla, distal common bile duct and pancreatic duct opening (Wirsung). The origin of the primary tumor, histopathological diagnosis and staging play a role in therapy and prognosis. WHO Classification of tumors of the digestive system, 2019 made changes in tumor staging following the system of the American Joint Committee on Cancer (AJCC), 8th edition. This study was conducted to determine types of pancreatic and ampullary malignancies, demographic distribution, location, profile, histomorphology and tumor staging changes in the 7th edition of the AJCC to 8th edition of the AJCC in the WHO Classification of tumors of the digestive system, 2019. The aim to determine clinicopathological profile of malignancies of ampulla of vater and pancreas as well as tumor staging changes based on AJCC edition 8.&#x0D; Methods&#x0D; Observational descriptive study on pancreatic and ampulla vater malignancy resection preparations at Department of Anatomic Pathology FKUI/RSCM, period January 2015 to December 2019 that met the inclusion criteria. Demographic evaluation, tumor location, histopathological profile and patient staging changes were carried out based on the 8th edition of AJCC.&#x0D; Results&#x0D; There were 27 cases of adenocarcinoma in ampulla of vater, 10 cases of pancreatic ductal adenocarcinoma, 1 case of mucinous cystic neoplasm with adenocarcinoma focus, 1 case of serous cystadenocarcinoma, 7 cases of PanNET, 2 cases of PanNEC, 3 cases of SPN, 1 case of MiNEN. Changes in pT were present in 29 cases. Changes in pN were present in 6 cases.&#x0D; Conclusion&#x0D; The diagnosis and pathological staging are in accordance with the 2019 WHO digestive system tumor classification. Tumor differentiation, presence or absence of lymphovascular invasion, perineural invasion and lymph node metastases and incision margin status are important histopathological parameters.

Physiological Impact of the Non-Nutritive Artificial Sweetener, Aspartame, and the Therapeutic Potential of Aqueous Extract of Phyllanthus niruri.

The present study investigated the impact of aspartame (ASP) on biochemical and histological profiles and the therapeutic potential of aqueous extract of Phyllanthus niruri (PN) in female Swiss albino mice (20 ± 2 g b. w.). ASP (40 mg/kg b. w.) and PN (100 mg/kg b. w.) were fed to the mice for a period of 30 and 60 days. A significant (P ≤ .01) decrease was observed in body weight and relative organ weight in ASP-treated mice. ASP induced a significant (P ≤ .01) increase in lipid profile, bilirubin, creatinine, and enzyme activity. Furthermore, histomorphological changes in the liver and kidney, including atrophy, lesions, and derangement of cellular structure, were observed in ASP-treated animals. However, animals treated with ASP and supplemented with aqueous extract of PN showed significant (P ≤ .01) improvements in enzyme activity and histomorphological changes in the liver and kidney. Aqueous extract of PN mitigates ASP-induced physiological effects, including liver and kidney function markers and histomorphological changes. The study suggests a need for identification of mechanisms of interaction of ASP and its breakdown products once ingested and the bioactive compounds of PN responsible for its therapeutic potential.

Correlations between the histopathological alterations in minor salivary glands and the clinically suspected Sjögren's syndrome.

In sicca syndrome patients the xerostomia, xerophthalmia and the serological findings may strongly suggest the autoimmune Sjögren's syndrome, but the histological findings in the labial salivary gland biopsies do not always justify the suspected diagnosis. The aim of this study was to compare the histomorphological changes and the clinical findings in patients with pathologically established Sjögren's syndrome and in cases with negative histology. A total of 133 labial biopsies have been retrospectively evaluated from 2015 to May 2022, and the characteristic Sjögren's lesions were found in 67 cases. According to the clinical data, 34 cases proved to be primary, and 33 were associated ("secondary") forms. In 66 cases, the histology did not justify Sjögren's syndrome; a significant acinar loss, fibrolipomatous infiltration, and mild sialadenitis had led to the clinical symptoms. In Sjögren's histologies, the acinar loss was detected in just 31.8% of cases, which might indicate that the diminished saliva production represents immune-mediated hypofunction rather than direct damage of the acini. This is the first systemic study in Hungary investigating the correlation between pathological alterations and clinical findings.

Oral administration of Turnera diffusa willd. ex Schult. extract ameliorates steroidogenesis and spermatogenesis impairment in the testes of rats with type-2 diabetes mellitus

Ethnopharmacological relevanceTurnera diffusa Willd. ex Schult. (T. diffusa) has traditionally been used to treat male reproductive dysfunction and have aphrodisiac properties.Aims of the study: This study aims to investigate the ability of T. diffusa to ameliorate the impairment in testicular steroidogenesis and spermatogenesis in DM that might help to improve testicular function, and subsequently restore male fertility. Materials and methodsDM-induced adult male rats were given 100 mg/kg/day and 200 mg/kg/day T. diffusa leaf extract orally for 28 consecutive days. Rats were then sacrificed; sperm and testes were harvested and sperm parameter analysis were performed. Histo-morphological changes in the testes were observed. Biochemical assays were performed to measure testosterone and testicular oxidative stress levels. Immunohistochemistry and double immunofluorescence were used to monitor oxidative stress and inflammation levels in testes as well as Sertoli and steroidogenic marker proteins’ expression. ResultsTreatment with T. diffusa restores sperm count, motility, and viability near normal and reduces sperm morphological abnormalities and sperm DNA fragmentation in diabetic rats. T. diffusa treatment also reduces testicular NOX-2 and lipid peroxidation levels, increases testicular antioxidant enzymes (SOD, CAT, and GPx) activities, ameliorates testicular inflammation via downregulating NF-ΚB, p-Ikkβ and TNF-α and upregulating IκBα expression. In diabetic rats, T. diffusa treatment increases testicular steroidogenic proteins (StAR, CYP11A1, SHBG, and ARA54, 3 and 17β-HSD) and plasma testosterone levels. Furthermore, in diabetic rats treated with T. diffusa, Sertoli cell marker proteins including Connexin 43, N-cadherin, and occludin levels in the testes were elevated. ConclusionT. diffusa treatment could help to ameliorate the detrimental effects of DM on the testes, thus this plant has potential to be used to restore male fertility.

Effect of dietary cinnamon supplementation on the growth in length, haematology, serum biochemistry, intestinal microbiota and enzymes and histomorphological changes of the intestine, liver and the kidney of Heteroclarias (Clarias gariepinus ♀ × Heterobra ♂)

The effect of dietary cinnamon supplementation on the growth, haematology, serum biochemistry, intestinal microbiota and enzymes and histomorphological changes of the intestine, liver and kidney of juvenile heteroclarias (Clarias gariepinus ♀ Heterobranchus bidorsalis ♂) was assessed in a 56-day feeding trial. Five 38% crude protein experimental diets were formulated to contain a cinnamon powder that was included at a rate of 0% (DT1), 0.5% (DT2), 1% (DT3),1,5% (DT4) and 2% (DT5). Each diet was allotted to a triplicate group of fish (n=15 fish/ replicate tank 8.55± 0.12 cm average length) in a completely randomized design. There was a significant increase (p&lt;0.05) in the growth performance parameters, haematological indices, antioxidant and hindgut enzyme activities with cinnamon supplementation. With DT4-fed having the highest values of these parameters. The cinnamon-fed groups all except the DT2-fed group had significantly (p&lt;0.05) lower total cholesterol levels than the control group. When compared to the control group, the blood glucose, blood urea nitrogen and creatinine level in the cinnamon-fed group decreases significantly (p&lt;0.05). The serum alanine aminotransferases (ALT) were statistically similar (p&gt;0.05) among the dietary groups. Bacteria counts were heightened significantly (p&lt;0.05) with cinnamon supplementation when compared to the control. The contrast was the results for fungi count with diversity indices in favour of DT4 dietary group. The amylase, cellulase and trypsin activity of the cinnamon-fed group was significantly (p&lt;0.05) elevated when compared to those of the control group. The intestine, liver and kidney microstructures were well preserved with no features of injury recorded.

A prospective study of spray cryotherapy in patients with COPD

AbstractObjectiveThe purpose of this study was to estimate the safety and efficacy of spray cryotherapy (SCT) in patients with chronic obstructive pulmonary disease (COPD).MethodsWe recruited patients aged 40–80 years with a confirmed diagnosis of COPD without serious complications (defined as two consecutive years of chronic cough and cough for at least 3 months with lung function forced expiratory volume in 1 s [FEV1]/forced vital capacity [FVC] &lt; 70% after inhaled bronchodilators and Global Initiative for Chronic Obstructive Lung Disease [GOLD] grade II–IV). The safety of this study was assessed by incidence of adverse events, and the efficacy was evaluated by St. George's Respiratory Questionnaire (SGRQ), COPD assessment test (CAT), Leicester Cough Questionnaire (LCQ) and changes in exercise capacity, histomorphology and lung function.ResultsEight patients eventually received three complete sessions of SCT, each lasting 41.10 ± 19.72 min. No adverse complications were recorded within 3 months after SCT. Clinically meaningful improvements in SGRQ, CAT, LCQ and exercise capacity were observed at 3 months after SCT: −15.75 ± 6.76 (95% confidence interval [CI] 10.10−21.40; p &lt; .001), −6.88 ± 3.91 (95% CI 3.61−10.14; p = .002), −18.13 ± 13.81 (95% CI 6.58−29.67; p = .008) and 72.75 ± 33.11 (95% CI −100.43 to −45.07; p &lt; .001), respectively. The density of airway epithelial goblet cells per unit area was significantly reduced (p &lt; .05), and lung function parameters such as ∆FVC, ∆FEV1 and ∆ peak expiratory flow (∆PEF) were improved (p = .007, .021 and .012).ConclusionsSCT is a safe and effective strategy to provide a better quality of life for COPD patients.

Histo morphological changes in gall bladder mucosa associated with gall stones

Background and Aims: Cholelithiasis is known to produce diverse histopathological changes in the gallbladder mucosa. In the present study, we aimed to find the correlation between various gallstone characteristics (i.e., number, size, and morphological type) with the type of mucosal response in gallbladder mucosa (i.e., inflammation, hyperplasia, metaplasia, and carcinoma). Methods: The present study was conducted prospectively on 250 patients undergoing cholecystectomy for symptomatic cholecystitis. Gallstones were assessed for various parameters, i.e., number, size, and morphological type. Gallbladder mucosa was subjected to histopathological examination. Sections were taken from body, fundus, and neck of gallbladder. Results: Of 250 cases, maximum type was of mixed stones (54%) and was multiple in number (46%). However, gallstone type and number are non-significant variables to produce precancerous lesions (i.e., hyperplasia and metaplasia). Statistically significant results were obtained while comparing the mucosal response with gallstone size (P = 0.012). Conclusion: As the gallstone size increases, the response in gallbladder mucosa changes from cholecystitis, hyperplasia, and metaplasia to carcinoma. Gallstone type and number are nonsignificant variables to produce precancerous lesions.

Histo-Morphogical Study of Full- Term Placenta in Selected Normotensive And Pre-eclamptic Women in Bangladesh

Pre–Eclampsia is a disorder in 2nd half of pregnancy, characterized by a combination of hypertension, proteinuria, and edema, secondary to decreased placental perfusion. Clinical studies suggest histo-morphological changes in the placenta of pre-eclamptic women compared to normotensive pregnant women. This study aimed to compare the histo- morphological changes of the placenta in selected pre-eclamptic and normotensive pregnant women. Methods: Two hundred twenty pregnant women were selected with inclusion and exclusion criteria from 3 different medical colleges and divided into two groups. A study group comprised 110 pre-eclamptic women, and a control group comprised 110 normotensive pregnant women. After delivery, tissue samples were collected from the placenta and prepared for histopathological studies by hematoxylin and eosin stain. The mean number of areas of syncytial knot formation, the mean number of areas of cytotrophoblast cell proliferation, the mean number of areas of fibrinoid necrosis, and the mean number of areas of hyalinized villi of pre-eclamptic and normal pregnant women were evaluated. We evaluated the morphology by weight, diameters, number of cotyledons, and placental infarcts. Results: The mean number of areas of syncytial knot formation, cytotrophoblastic cell proliferation, fibrinoid necrosis, and hyalinised villi were significantly higher in the study group compared to the control group. The mean weights, diameters, and the number of cotyledons were considerably lower in the study group compared to the control group. Moreover, the number of infarcted areas was significantly higher in the placentas of pre-eclamptic women. Conclusion: There are histomorphologic changes in the placenta of pre-eclamptic women. J Dhaka Med Coll. 2022; 31(1) : 37-45

Comparative effects of finasteride and laser-irradiated silver nanoparticles on testicular function and histology in testosterone induced benign prostatic hyperplasia in rats

AimsThe objective of this study was to compare the effects of finasteride, a medication used to treat benign prostatic hyperplasia (BPH), and laser irradiated silver nanoparticles (AgNPs), a potential candidate for BPH therapy (Sanchez-Salas, 2017; Marghani et al., 2022) [1,2], on the sex hormone profiles, sperm quality, steroidogenesis, testicular oxidative stress, and histomorphology changes in BPH rats. Materials and methodsBPH was induced in male Sprague-Dawley (SD) rats via intramuscular (i.m.) injection of 5 mg/kg BW testosterone propionate (TP) for 14 days. Once the BPH model was induced, rats were divided into four groups (n = 6) as follows: the control group; the BPH group; the BPH/Fina group, which received 5 mg/kg BW finasteride by oral gavage daily for 14 days; and the BPH/AgNPs group, which received a daily intraperitoneal (i.p.) injection of 50 mg/kg BW AgNPs, followed by 5min of exposure to a 532 nm NIR laser in the prostatic area for the constitutive 14 days. Key findingsOn day 14, the BPH rats had a significant increase in prostate specific antigen (PSA), dihydrotestosterone, and prostate weights, while testicular weights and sperm quality were significantly lower than in the control rats. On day 28, laser irradiated AgNps treated BPH rats showed improved sex hormone balance, testicular weights, sperm quality, steroidogenesis, and an ameliorative effect on testicular histopathology compared to finasteride. SignificanceSurprisingly, these findings suggest that laser irradiated AgNPs can be used as an alternative therapy to finasteride for the treatment of BPH without causing negative effects on the testes.

Cypermethrin induced liver oxidative DNA damage via the JNK/c-Jun pathway

To clarify the adverse effect of cypermethrin(CYP) on the liver and explore the underlying role of the MAPK pathway. Twenty-four Sprague-Dawley(SD) rats were exposed to 0, 5, 10 and 20 mg/(kg·d) β-CYP by gavage for 31 days. Histomorphological and ultrastructural changes were evaluated by the hematoxylin &amp; eosin(HE) staining and transmission electron microscope(TEM). Levels of MDA and 8-OHdG were detected by ELISA. Expressions of p-JNK and γ-H2A. X were assessed by IHC and IF respectively. RT-PCR was performed to examine mRNA levels of GPx1, GPx4, SOD1, and SOD2 in rat testes. Western blot was conducted to determine protein expressions of GPx1, SOD2, CAT, γ-H2A. X, and the MAPK pathway-associated proteins in rat testes. After β-CYP exposure, the histomorphology and ultrastructures of rat livers were abnormally altered, as evidenced by hepatic sinusoidal dilation, hepatic plate space formation, mitochondrial crest fracture, etc. Moreover, β-CYP induced mRNA levels of GPx1, GPx4, SOD1 and SOD2, as well as protein expressions of GPx1 and SOD2 in the liver. Compared to the control, GPx1 and SOD2 protein expressions were decreased by 57.9% and 50.0%(P&lt;0.05), whereas the MDA level was increased by 56.2%(P&lt;0.05) in the high-dose group. Additionally, the JNK/c-Jun pathway, one of MAPK pathways, in the liver was activated by β-CYP, as shown by the increase of JNK and c-Jun phosphorylation, and protein expressions of p-JNK and p-c-Jun in the high-dose group were elevated by 47.7% and 46.5%(P&lt;0.05) in comparison to the control, but the ERK and p38 pathways were not affected after β-CYP exposure. Furthermore, β-CYP promoted 8-OHdG and γ-H2A. X expressions in the liver. Compared to the control, γ-H2A. X protein expression in the mid-and high-dose group was upregulated by 16.9% and 33.9%(P&lt;0.05), respectively. Cypermethrin had detrimental effects on the liver. CYP not only directly altered liver histomorphology and ultrastructures, but also caused oxidative stress, which activated the JNK/c-Jun pathway, finally inducing the DNA damage.

THE INVESTIGATION OF THE EFFECTS OF VITAMIN D AND OMEGA 3 ON VENA CAVA

Objective: Vitamin D has a protective role in the cardiovascular system and it affects blood pressure. Omega-3 fatty acids are dietary fats gained from fish and plant oils and involve in coronary heart disease and other cardiovascular complications. The aim of the study was to investigate the effects of Vitamin D and Omega 3 on the vascular structure at the cellular level.&#x0D; &#x0D; Material and Methods: In the current study, a total of 24 rats were divided into 4 groups. Each group contained 6 animals. The groups are as follows; control, vitamin D, Omega 3; and combined Vitamin D and Omega 3. Vena cava samples from all groups were obtained and stained with hematoxylin and eosin (H&amp;E) for histological alternations. Additionally, endothelial and vascular functions were investigated immunohistochemically.&#x0D; &#x0D; Results: The H&amp;E staining revealed that the treatment of either Vitamin D or Omega 3 did not cause histomorphological changes in the structure of the vena cava under normal conditions. The immunoexpression of inducible nitric oxide synthase was decreased and vascular endothelial growth factor was increased in the vena cava of rats with the combined treatment of Vitamin D and Omega 3.&#x0D; &#x0D; Conclusion: In conclusion, combined supplements of Vitamin D and Omega 3 did not have harmful effects on the blood vessel however further studies should be performed to determine the beneficial effects of these supplements.

Study of the corrosion behavior and mechanism of a hot-dipping Zn–6Al–3Mg alloy coating in 3.5 wt% neutral NaCl solution

In this study, the microstructure of a hot-dipping Zn–6Al–3Mg alloy coating was characterized and its histomorphological, electrochemical and corrosion products changes when submerged in a 3.5 wt% NaCl solution were investigated. Further, the effects of different microstructures on the corrosion resistance of the coating and the corrosion mechanism were investigated. Different microstructures lead to significant differences in the corrosion resistance of the coating, indicating the different roles microstructures play in this process. The ternary eutectic phase was preferentially corroded, where corrosion products were formed preferentially and gradually covered the coating surface. This prevented the corrosive medium from further attacking the coating, improving its corrosion resistance.

Prevention and Treatment of Acute Coronary Artery Syndrome

Acute coronary syndrome(ACS) refers to a group of clinical symptoms caused by acute myocardial ischemia, including unstable angina pectoris, acute myocardial infarction, and sudden ischemic death. In recent years, studies have shown that the main mechanism of its occurrence is the rupture of plaque and the consequent intravascular thrombosis. Multiple factors may lead to the occurrence of the disease. However, the mechanism of the histomorphological change from pre-fragmentation to post-fragmentation is still unclear. Vasospasm may also play an important role. Prevention of plaque formation and rupture or prevention of thrombosis by antithrombotic therapy is effective in reducing the incidence of acute coronary syndrome, and coronary intervention also reduces the mortality of acute coronary syndrome. Some new therapies, such as gene therapy, are still in the experimental stage. At the same time, people should improve the awareness of disease prevention, and the most active prevention is the best means of disease treatment.

Age Related Cytoarchitectural Comparison of Histopathological Changes in Thyroid Nodule

Objective: To compare age related histomorphological changes through cytology (Bethesda category) and histopathology in thyroid nodules. Study design and setting: This cross sectional study was conducted at the Department of Anatomy, BMSI, JPMC Karachi from 31-March-2021 to 31-August-2021. Methodology: Total n=120 patients of all ages, genders and ethnicities that underwent surgery for thyroid nodules were included after taking duly signed consent. On the basis of cytological reports (FNAC) patients were grouped according to age and gender. Processed paraffin blocks were sectioned at a thickness of 5µm and then were stained with Haematoxylin and Eosin (H&amp;E) for histological evaluation. Masson trichrome stain was used to observe fibrosis, that was measured through Image J Fiji software. Results: We evaluated that out of 120 patients, male to female ratio was 1:5.67. Among various ethnic groups; Urdu speaking (40.8%) with mean age of 40.3% were commonly affected. Histopathological examination revealed that most common benign lesion was colloid nodular hyperplasia and papillary carcinoma was the most common malignant lesion. Highest percentage of fibrosis (36%) was seen in middle aged participants (p-0.045). Basement membrane thickness with lowest percentage (12%) was observed in younger age participants while highest (31%) was measured in older aged participants (p-0.001). Accuracy score of FNAC showed sensitivity 59%, specificity 96.6%, accuracy 86%, positive predictive value (PPV) 86% and negative predictive value (NPV) 87%. Conclusion: With progression in age thyroid gland undergo histomorphological changes, early diagnosis will help in categorical nomenclature and its implications for subsequent management of patients with thyroid lesions

Evaluating the histomorphological and biochemical changes induced by Tributyltin Chloride on pituitary-testicular axis of adult albino rats and the possible ameliorative role of hesperidin

ABSTRACT This study was performed to explore in detail the toxic effects of Tributyltin Chloride (TBT) on the pituitary-testicular axis and the possible amelioration with Hesperidin. Seventy-two adult male albino rats were divided into four groups: Control group (I), TBT-treated group (II), TBT+Hesperidin group (III), and Recovery group (IV). Body and testicular weights were measured. Blood samples were taken to estimate serum levels of testosterone, FSH and LH hormones by enzyme-linked immunosorbent assay (ELISA). Malondialdehyde (MDA) level was measured in testes homogenates. Tissue samples from the pituitary glands and testes were processed for light, electron microscope examination, and immunohistochemical detection of anti-FSH, and Ki67 proteins. Results showed a statistically significant decrease in testicular weight, serum testosterone, FSH and LH levels and a significant increase in tissue MDA in the TBT group when compared to the control group. TBT treatment caused severe histopathological changes with decreased area percent of PAS-stained basophils, and anti FSH immuno-stained gonadotrophs in the pituitary gland. The testes of group II also showed marked tissue damage, cell loss with decreased epithelial height and decreased number of proliferating spermatogenic cells. Hesperidin supplementation with TBT proved significant amelioration of the previously mentioned parameters in both glands which could improve male fertility. In conclusion: The flavonoid Hesperidin has the potential to protect against the reproductive damage induced by TBT in susceptible individuals.

Prenatal anesthetic exposure and offspring neurodevelopmental outcomes-A narrative review.

While it is common for pregnant women to take anesthesia during surgery, the effects of prenatal anesthesia exposure (PAE) on the long-term neurodevelopment of the offspring remain to be clarified. Preclinical animal research has shown that in utero anesthetic exposure causes neurotoxicity in newborns, which is mainly characterized by histomorphological changes and altered learning and memory abilities. Regional birth cohort studies that are based on databases are currently the most convenient and popular types of clinical studies. Specialized questionnaires and scales are usually employed in these studies for the screening and diagnosis of neurodevelopmental disorders in the offspring. The time intervals between the intrauterine exposure and the onset of developmental outcomes often vary over several years and accommodate a large number of confounding factors, which have an even greater impact on the neurodevelopment of the offspring than prenatal anesthesia itself. This narrative review summarized the progress in prenatal anesthetic exposure and neurodevelopmental outcomes in the offspring from animal experimental research and clinical studies and provided a brief introduction to assess the neurodevelopment in children and potential confounding factors.

Histomorphological Observations in Reproductive Organs of Wistar Rats Administered with Aqueous Leaf Extract of Momordica charantia

Effort to explore the adverse effect of the plant, Momordica charantia on mammals has remained inadequate despite previous attempt by earlier investigators. More glaring is the paucity of information on the histomorphological effect of the plant’s aqueous leaf extract hence the need to determine possible alteration of tissue structures in reproductive organs of adult Wistar rats which may affect their functions.&#x0D; This study aimed at determining the effect of Momordica charantia aqueous leaf extract (MCALE) on the reproductive organs of experimental adult Wistar rats.&#x0D; Materials and Methods: Twenty five rats (male and female) weighing 180-200g were randomly divided into five (5) groups of five rats each. The experimental groups, A to D were fed on standard diet and administered with 100, 200, 400 and 800 mg/kg body weight /day of MCE orally using gavage for 30 days. Rats in the control groups were fed on standard diet and physiological saline orally. Organs were harvested, fixed in 10% neutral buffered formalin (ovaries) and Bouin’s fluid (testes), embedded in molten paraffin wax, sectioned with a rotary microtome and stained with the haematoxylin and eosin technique. Stained slides were examined using the Olympus microscope. &#x0D; Results: Sections of ovaries administered 100 mg/kg of the extract showed vesicular spaces in corpus luteum and enlarged blood vessels. Sections treated with 200 mg/kg revealed follicular cyst and mild vacuolation of zona granulosa. Sections of the ovaries administered 400 mg/kg revealed degenerative changes, follicular cyst, mild vacuolation and reduction of zona granulosa layer while those treated with 800 mg/kg showed severe vacuolation of the zona granulosa layer. &#x0D; Conclusion: Momordica charantia caused histomorphological changes in ovaries of Wistar rats which could cause hormonal imbalance and infertility in females. No histomorphological changes were observed in male testes.

Polystyrene nanoplastics lead to ferroptosis in the lungs

IntroductionIt has been shown that polystyrenenanoplastic (PS-NP) exposure induces toxicity in the lungs. ObjectivesThis study aims to provide foundational evidence to corroborate that ferroptosis and abnormal HIF-1α activity are the main factors contributing to pulmonary dysfunction induced by PS-NP exposure. MethodsFifty male and female C57BL/6 mice were exposed to distilled water or 100 nm or 200 nm PS-NPs via intratracheal instillation for 7 consecutive days. Hematoxylin and eosin (H&E) and Masson trichrome staining were performed to observe the histomorphological changes in the lungs. To clarify the mechanisms of PS-NP-induced lung injury, we used 100 μg/ml, 200 μg/ml and 400 μg/ml 100 or 200 nm PS-NPs to treat the human lung bronchial epithelial cell line BEAS-2B for 24 h. RNA sequencing (RNA-seq) of BEAS-2B cells was performed following exposure. The levels of glutathione, malondialdehyde, ferrous iron (Fe2+), and reactive oxygen species (ROS) were measured. The expression levels of ferroptotic proteins were detected in BEAS-2B cells and lung tissues by Western blotting. Western blotting, immunohistochemistry, and immunofluorescence were used to evaluate the HIF-1α/HO-1 signaling pathway activity. ResultsH&E staining revealed substantial perivascular lymphocytic inflammation in a bronchiolocentric pattern, and Masson trichrome staining demonstrated critical collagen deposits in the lungs after PS-NP exposure. RNA-seq revealed that the differentially expressed genes in PS-NP-exposed BEAS-2B cells were enriched in lipid metabolism and iron ion binding processes. After PS-NP exposure, the levels of malondialdehyde, Fe2+, and ROS were increased, but glutathione level was decreased. The expression levels of ferroptotic proteins were altered significantly. These results verified that PS-NP exposure led to pulmonary injury through ferroptosis. Finally, we discovered that the HIF-1α/HO-1 signaling pathway played an important role in regulating ferroptosis in the PS-NP-exposed lung injury. ConclusionPS-NP exposure caused ferroptosis in bronchial epithelial cells by activating the HIF-1α/HO-1 signaling pathway, and eventually led to lung injury.