Histological Changes In Testis Research Articles

Study of Histological Changes of Testis in Rats of Different Ages When Exposed to Frequency 10GHZ Within The X-Band Microwave

This study investigates the impact of 10 GHz microwave exposure, within the X-band range, on rat testicular tissue and sperm development across different age groups. Laboratory rats were divided into a control group of six, which was not exposed to microwaves, and a treatment group of 18, further split into three age-based subgroups (8–12 weeks, 12–16 weeks, and 16–20 weeks), each containing six rats exposed to 10 GHz microwaves for 2.5 hours daily over five weeks. The findings revealed no histological changes in the control group. However, in the treated groups, significant alterations were observed: the youngest group showed a lack of sperm formation and loss of spermatocytes; the middle group displayed malformed sperm and reduced numbers; and the oldest group experienced a decrease in sperm count and minor shape alterations. Additionally, after five weeks, all treated groups exhibited damage to the testicular interstitial tissue, including ruptures and space formation between seminiferous tubules. This study suggests potential risks of microwave exposure on reproductive health and lays the groundwork for future research in this area.

HISTOLOGICAL CHANGES IN TESTIS OF RABBIT BUCKS FED WITH DIET CONTAINING RAW OR FERMENTED COTTONSEED CAKE SUPPLEMENTED WITH GINGER

The aim of this study was to evaluate the histological changes in testis of rabbit bucks (New Zealand White X Chinchilla) fed with raw or fermented cottonseed cake (CSC) - based diets supplemented with ginger. Solid state fermentation (SSF) was employed to biodegrade CSC using Aspergilus niger. Thirty (30) weaner rabbit bucks aged between 5 - 6 weeks were acclimatized for two weeks and allocated to five dietary treatments with equal number of animals (n=6) per treatment in a 2x2 factorial arrangement within a completely randomized design (CRD). Treatment 1, (control) had soybean meal (SBM) as protein source; other four treatments were divided into two groups. The first group comprised raw or fermented CSC without ginger supplementation while the second group comprised raw or fermented CSC with ginger supplementation. The SBM was completely replaced with CSC weight for weight in the four treatments. Ginger was supplemented at a dosage of 30mg/kg diet. Feeding trial lasted for 12 weeks. Testis samples were processed for histological evaluation. The photomicrographs of the testis revealed that feeding raw CSC diets without ginger supplementation resulted in severe testicular damage. Either the fermentation of CSC by A. niger or ginger supplementation, or their combination exhibited the potential to greatly ameliorate the adverse effects. It was therefore, concluded that feeding CSC to rabbit buck intended for breeding purpose should either be fermented with A. niger or supplemented with ginger or their combination

BRD7 facilitates ferroptosis via modulating clusterin promoter hypermethylation and suppressing AMPK signaling in diabetes-induced testicular damage

BackgroundDiabetes mellitus (DM)-induced testicular damage is associated with sexual dysfunction and male infertility in DM patients. However, the pathogenesis of DM-induced testicular damage remains largely undefined.MethodsA streptozotocin (STZ)-induced diabetic model and high glucose (HG)-treated in vitro diabetic model were established. The histological changes of testes were assessed by H&E staining. Serum testosterone, iron, MDA and GSH levels were detected using commercial kits. Cell viability and lipid peroxidation was monitored by MTT assay and BODIPY 581/591 C11 staining, respectively. qRT-PCR, immunohistochemistry (IHC) or Western blotting were employed to detect the levels of BRD7, Clusterin, EZH2 and AMPK signaling molecules. The associations among BRD7, EZH2 and DNMT3a were detected by co-IP, and the transcriptional regulation of Clusterin was monitored by methylation-specific PCR (MSP) and ChIP assay.ResultsFerroptosis was associated with DM-induced testicular damage in STZ mice and HG-treated GC-1spg cells, and this was accompanied with the upregulation of BRD7. Knockdown of BRD7 suppressed HG-induced ferroptosis, as well as HG-induced Clusterin promoter methylation and HG-inactivated AMPK signaling in GC-1spg cells. Mechanistical studies revealed that BRD7 directly bound to EZH2 and regulated Clusterin promoter methylation via recruiting DNMT3a. Knockdown of Clusterin or inactivation of AMPK signaling reverses BRD7 silencing-suppressed ferroptosis in GC-1spg cells. In vivo findings showed that lack of BRD7 protected against diabetes-induced testicular damage and ferroptosis via increasing Clusterin expression and activating AMPK signaling.ConclusionBRD7 suppressed Clusterin expression via modulating Clusterin promoter hypermethylation in an EZH2 dependent manner, thereby suppressing AMPK signaling to facilitate ferroptosis and induce diabetes-associated testicular damage.

Biochemical Changes of Mancozeb-Induced Alternations in Testes and Thyroid gland of Male Albino Rats

Mancozeb manganese ethylenebis dithiocarbamate polymeric complex – with zinc salts is a very important protective non-systemicfungicide, classified as an ethylenebisdithiocarbamate fungicide. It is used to control fungal diseases for a wide variety of crops because its broad-spectrum fungicidal effects and high compatibility with agrochemicals. The objective of this study was to investigate the effects of the fungicide mancozeb at different doses on some biochemical parameters, reproductive performance and histological changes in testes and thyroid gland. The low dose equal 1/7 of LD50 manc-d1 and high dose equal 1/3.5 of LD50 manc-d1. Doses of mancozeb (manc-d1 and manc-d2) adjusted according to rat's body weights. The results showed that mancozeb significantly (P<0.05) decreased plasma testosterone level, sperm count, viability, motility, and significantly (P<0.05) increased abnormal sperms, altered acrosome and abnormal DNA. Treatment of rats with manc-d1 and manc-d2 significantly (P<0.05) decreased T3. Also, T4 significantly (P<0.05) decreased in the group treated with manc-d2. Treatment of rats with manc-d1 and manc-d2 significantly (P<0.05) increased TSH. Furthermore, the histological investigation revealed that exposure to mancozeb reduced number of mature spermatozoa, necrosis and basal vacuoles observed in some tubules. On the other hand, mancozeb reduce colloid in most follicles resulting in desquamation of the follicular epithelium into the lumen of the thyroid follicles. In conclusion, despite mancozeb exhibit low acute toxicity, it has been shown to cause detrimental effects on reproduction, thyroid gland and its secretion. For this reason, it should be necessary to be careful when using mancozeb in agricultural areas and should take precautions.

Sperm parameters and histological changes in testes of cadmium-exposed rats treated with Hibiscus sabdarrifa L. anthocyanins

Given increase in infertility due to environmental pollutants, this study examined changes in sperm parameters and testicular histology of cadmium- exposed rats treated with Hibiscus sabdarrifa L. anthocyanins (HSA). Adult male Wistar rats were divided into 9 groups: A (Control), B (Cd), C (HS Aq Extract), D (Low Dose HSA), E (High Dose HSA), F (Low Dose (HSA Pre Cd), G (High Dose HSA Pre Cd), H (Low Dose HSA Post Cd), I (High Dose HSA Post Cd) and treated in two experimental periods (5 days acute toxicity study and 15 days chronic toxicity study). Exposure to Cd alone significantly (p<0.05) reduced sperm volume, motility, morphology and count for both treatment periods compared to the control, and rats administered high and low doses of HSA alone. However, the administration of high and low doses of HSA to Cd-exposed rats significantly altered (p<0.05) these parameters compared to rats exposed to Cd alone. Sections of the testes from control showed several seminiferous tubules disposed within an interstitium composed of interstitial cells of Leydig indicating normalcy. Conversely, section of Rats testes from rats exposed to Cd showed several small sized seminiferous tubules with predominantly roundish architecture indicating great congestion of interstitial blood vessels. The study showed that exposure to Cd induced alterations in sperm parameters and also caused histological changes in the testes of rats. However, the administration of high and low doses of HSA to Cd-exposed rats before and after Cd exposure significantly altered these parameters compared to rats exposed to Cd alone.

Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice.

Hyperglycemia associated with hyper- or hypo-insulinemia is a hallmark of type 2 diabetes mellitus, which is firmly linked to decreased male infertility. Recently, bee venom (BV) has shown potential health prosperities, including antidiabetic; however, no study focuses on the effect of BV on male fertility in diabetic conditions. This study aimed to detect the effect of BV on histological and hormonal alteration of the testis in diabetic mice. Twenty adult male mice were selected and assigned to four groups: Control, diabetic (150 mg/kg alloxan), BV1 (diabetic + 0.5 mg/kg BV), and BV2 (diabetic + 1 mg/kg BV). After 35 days, the serum levels of glucose, insulin, testosterone, follicular-stimulating hormone, luteinizing hormone, and prolactin were estimated. The histological structure of the testes was also evaluated. Alloxan-induced hyperglycemia and decreased insulin concentrations were reversed significantly by BV. Furthermore, diabetic mice exhibited various alterations in fertility hormones, while these disturbances were improved considerably to normal concentrations by BV. Similarly, alloxan-induced changes in sperm and testis histological parameters such as motility, viability, abnormality, sperm count, the number and diameter of seminiferous tubules, and the number of Leydig and Sertoli cells were significantly ameliorated to the normal condition by BV. Changes in the number, size, and shape of seminiferous tubules, the number of Leydig and Sertoli cells, and initial degeneration and vacuolization in interstitial cells and spermatogonia and spermatocyte were seen in diabetic mice. All these changes were shifted almost to normal structure by BV. The BV could be used as an alternative therapeutic agent that manages the markers related to diabetic conditions concomitant with the improved histological structure of the testes and hormone production to accelerate male fertility.

Toxicity evaluation induced by single and 28-days repeated exposure of withametelin and daturaolone in Sprague Dawley rats.

Safe preclinical dose determination is predictive of human toxicity and can have a profound impact on the overall progress of the compound in early drug discovery process. In this respect, current study sought to investigate for the first time the acute and subacute oral toxicity of two pharmacologically active natural compounds i.e., withametelin and daturaolone in Sprague Dawley rats following OECD guideline 420 and 407, respectively. As per acute toxicity studies, withametelin and daturaolone were characterized as Globally Harmonized System (GHS) category 4 and 5 compounds, respectively. Sub-acute daily dose of withametelin was 5, 2.5, and 1.25 mg/kg but, for daturaolone, it was 10, 5, and 2.5 mg/kg. High dose (5 and 2.5 mg/kg) withametelin groups showed dose dependent changes in the general, hematological, biochemical and histopathological parameters in both sexes, the most prominent being hyperthyroidism while no toxicity was observed at lower doses (1.25 and 0.75 mg/kg), No Observable Adverse Effect Level (NOAEL) being 1.25 mg/kg. Daturaolone was comparatively safer and showed dose dependent significant changes in hepatic enzyme (Alanine Transaminase), bilirubin, creatinine, and glucose levels while histological changes in testes were also observed. Lower doses (5, 2.5, and 1.25 mg/kg) of daturaolone showed no significant toxic effects and 5 mg/kg was declared as its NOAEL. Depending upon our findings, starting effective oral dose levels of 1.25 mg/kg/day for withametelin and 5 mg/kg/day for daturaolone are proposed for repeated dose (up to 28 days) preclinical pharmacological evaluation models. Long term studies with more behavioral, biochemical, histopathological and hormonal parameters are proposed to strengthen the findings.

Aryl hydrocarbon receptor agonist diuron and its metabolites cause reproductive disorders in male marine medaka (Oryzias melastigma)

Diuron, a widely used phenylurea herbicide, has been frequently detected in marine organism and seawater all over the world. But the understanding of potential damage of diuron on reproduction in marine fish is currently not enough. Herein, marine medaka (Oryzias melastigma) were continuously exposed to 0, 5, 50, 500, and 5000 ng/L diuron from embryo (0 dpf) to adult (180 dpf) stage. The results suggested that diuron had an adverse influence on male reproduction for marine medaka, including decreased gonado somatic index, histological changes of testes, decreased mobility of sperm, and reduced fecundity through disrupting the balance of sex hormone and genes expression related to hypothalamus-pituitary-gonadal-liver (HPGL) axis. The reduced fecundity was reflected in abnormal sexual behaviors, further inhibited growth and development of F1 embryo and larvae. Moreover, the proportion of diuron metabolites (DCPMU and DCPU) was increased in fish, but the proportion of diuron was decreased with the increasing of exposure concentration. Diuron, DCPMU, and DCPU was identified as aryl hydrocarbon receptor agonist (AhR) agonist using in silico and in vivo models. DCPMU and DCPU induced the gene expression of AhR signaling and metabolizing enzymes (such as cyp1a1) in the livers. A great deal of major metabolites affected various organs related to HPGL axis of male marine medaka and led to serious reproductive disorders. Consequently, it reveals that long-term exposure to environmentally relevant concentrations of diuron and even AhR agonist pesticides pose a potential ecological risk for marine fish.

Cyclophosphamide Induced Histological Changes in Testis of Swiss Albino Mice

Background: Cancer is one of the leading causes for morbidity and mortality. The most often used anticancer drug is Cyclophosphamide, the continuous usage of which may affect the fertility outcome in young boys. Henceforth an attempt is made in this experimental animal study design on swiss albino mice to look into the histological changes in the testis tissue post exposure to the Cyclophosphamide. Subjects & Methods: Healthy male Swiss albino adult mice (n=43), which were 6 to 8 weeks old, with average weight of 32±5 g were obtained from Central Animal Research Facility, Manipal were used for the study. They were maintained in well ventilated polypropylene cages containing paddy husk. The adult male mice were divided into two groups, control (n=7) and test (n=36). In the test group, the mice were injected with Cyclophosphamide at variable doses as per body weight. From each group certain no. of mice was sacrificed on different days. A parallel control group was kept for each of the treatment intervals. Similarly, the 12 prepubertal male mice, control (n=3) and test (n=9) which were of 2 weeks old with average weight of 8±2 g were also considered. The testicular tissue was then extracted and used in histological study. Results: A significant damage was noticed in the spermatogenic cells of the testicular epithelium. However Leydig cells were least affected. Conclusion: The experimental design studied here throws light on dose and time dependent toxic effect of one of the most commonly used anticancer drug- cyclophosphamide. Keywords: Cyclophosphamide, Testis histology, Swiss Albino mice.

Histological Changes in Testis of Adult Wistar Albino Rats - Nicotine-Induced Testicular Damages and the Protective Effects of Methanolic Extract of Sphaeranthus Amaranthoides

Nicotine which is the most dynamic substance present in tobacco is the vital cause of damaging the tissues and oxidative stress that in turn is the principal cause of numerous diseases. The succession and severity of nicotine toxicity which may be adjourned by the natural antioxidants may cause serious health, knock. We have scrutinized the consequences of methanolic extract of Sphaeranthus amaranthoides (SAE) on nicotine-induced reproductive toxicity, oxidative damage, and hepatotoxicity in adult Wistar male albino rats. In four groups 24 rats were contingently split up. Control, Nicotine N (0.4 mg/kg. i.p) methanolic extract of Sphaeranthus amaranthoides (SAE) and (N+ SAE) group. In order to compute the levels of testosterone and to measure the hematological and oxidative stress parameters, blood samples were collected after 48 days. The reproductive organs were weighed and taken for semen analysis and histopathology study. We could see decrease in the levels of testosterone, weights of reproductive organs, and even in the morphological features of sperms due to reproductive toxicity of Nicotine. On histological examination, testis showed atrophy, degenerative alterations of spermatogenesis in numerous seminiferous tubules together with increased interstitial spaces and a decrease in the number of Leydig cells. SAE played a defensive role against nicotine which persuaded the reproductive effects by enhancing the spermatogenesis, semen quality, and testicular histological damage.

The ameliorative effect of ellagic acid on di-(2-ethylhexyl) phthalate-induced testicular structural alterations, oxidative stress, inflammation and sperm damages in adult mice

BackgroundPhthalates such as di (2-ethylhexyl) phthalate (DEHP) are well known exogenous substances, disrupting reproductive system function and structure. The current research demonstrated the effect of ellagic acid (EA) on DEHP-induced testicular injury in mice.MethodsThirty-five healthy adult male mice were randomly divided to five groups; normal saline receiving group, DEHP (2 g/kg/day, dissolved in corn oil, p.o.) receiving group, DEHP (2 g/kg/day, dissolved in corn oil, p.o.) and EA receiving groups (25, 50 and 100 mg/kg/day, p.o.). Treatment duration of animals was 14 days. Body and testes weights and sperm characteristics and histological changes of testes were evaluated. Serum testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were analyzed. In the testicular tissue, oxidative/nitrosative stress markers and inflammatory cytokine levels were measured.ResultsEllagic acid significantly reduced DEHP-induced reduction of body and testes weights. The DEHP-induced reduction of spermatogonia, primary spermatocyte and sertoli cells numbers as well as reduction of sperm vitality and progressive motility were reversed by EA. Furthermore, EA inhibited DEHP-induced alterations in serum hormone levels. These effects were associated with the reduction of DEHP-induced increased level of oxidative stress and inflammatory responses.ConclusionsEllagic acid considerably inhibits testicular toxicity of DEHP through reducing oxidative/nitrosative stress and inflammatory responses. Our data suggest that EA may be considered as a promising agent to inhibit male reproductive toxicity induced by endocrine disrupting chemicals such as DEHP.

Testicular fixation and its effect on ipsilateral and contralateral testis in prepubertal rat model

BackgroundWhile performing orchidopexy, various suture materials or fibrin glues are used to achieve testicular fixation. This study was designed to assess the histological changes in testis after orchidopexy using fibrin glue and suture material.MethodsMale Wistar rats (n = 80) were divided randomly into four groups. Group I, (n = 20): sham operation, Group II (n = 20): Dartos Pouch (DP), Group III (n = 20): Transtunical fixation (TF), Group IV (n = 20): Tissue Adhesive (TA). Ipsilateral and contralateral testicular histology was assessed at 70 and 120 days of life after sacrificing animals by using thiopental sodium intraperitoneally at a dose of 100 mg/kg.ResultsMorphologically, at day 70, contralateral testis in Group III had a significant (p value 0.046) decrease testicular width (0.92 ± 0.01 vs 1.24 ± 0.39 cm). At 120 of life, Group I, II, III, and IV had a significant (p value < 0.001 each) decrease testicular width and weight in ipsilateral and decrease testicular length (p value 0.002) in contralateral testis. Histologically, mean seminiferous tubular diameter and DNA flow cytometry had a significant (p value < 0.001) decrease in size in Group I, II, III, and IV both ipsilateral as well as contralateral testis. Intergroup comparison at 70 and 120 days of life showed a significant decrease in seminiferous tubular diameter in Group II, III and IV and in Johnsen maturation score, seminiferous tubular diameter, DNA flow cytometry in Group I, II, III, and IV.ConclusionsDartos Pouch is most suitable procedure for treatment of orchidopexy. Suture fixation must be avoided and if the need arises then instead of suture materials, fibrin glue should be used for testicular fixation.

Comparison of sperm parameters and testis histological structure in lake frog (Pelophylax ridibundus) from areas with different level of anthropogenic pollution

The aim. The aim of the work was to study the indicators of spermatogenesis in the lake frog, Pelophylax ridibundus (Pallas, 1771), in the zone of the influence of emissions from the Burshtyn Thermal Power Plant (TPP).&#x0D; Materials and methods. Urinal sperm and testes samples were taken from male frogs under conditions of anthropogenic pollution (Burshtyn industrial area). Such parameters as sperm concentration in ejaculate, the number of live spermatozoa, total amount of actively mobile forms, and percentage of spermatozoa with progressive motion were determined by calculations in the hemocytometer. Testis samples were processed for routine histological method. The diameter of seminiferous tubules and histological abnormalities were studied.&#x0D; Results. Studies have revealed that near the Burshtyn TPP industrial site, the sperm concentration in the sperm sample and the total number of motile spermatozoa of the lake frogs significantly decreased. The number of dead spermatozoa increases. Amphibians from polluted sites had histological abnormalities in testes such as dilation of the seminiferous tubules with sparse sperm. At the same time, the diameter of the seminiferous tubules in the animals from polluted site significantly decreased.&#x0D; Conclusions. The impact of the emissions from the Burshtyn thermal power plant has an expressive reprotoxic effect that is manifested by depressive changes in testes tissue and gametes. The reproductive system of the lake frog is quite sensitive to environmental pollution. Sperm parameters and histological changes in testes are informative biomarkers and can be used for bioindication of technogenically transformed territories

Low semen quality and adverse histological changes in testes of adult male mice treated with bee venom (Apis mellifera).

Background:Male infertility has been on the rise since the past seven decades. Recently, in Libya, bee venom therapy (BVT) has become a popular method among alternative healthcare practitioners for treating male infertility. However, a literature search did not find any published studies that investigated the use of BVT for infertility treatment.Aim:To investigate the effect of bee venom on the male reproductive status through measurements of semen quality parameters and testicular histological changes in adult male mice.Methods:A total of 48 male mice were randomly divided into three experimental groups (which were subdivided into two subgroups with eight mice each) as follows: control, bee venom sting (BVS), and bee venom injection (BVI). The normal control subgroup mice were not subjected to any treatment, while the vehicle control subgroup mice were injected (i.p.) with 200 μl of 0.9% saline solution. In the BVS-treated subgroups, each mouse was stung by one live bee for five times (BVS-5) or seven times (BVS-7) every third day for 2 or 3 weeks. While each mouse in the BVI-treated subgroups received 23 μg/kg in a dose volume of 200 μl BVIs (i.p.) for five times (BVI-5) or seven times (BVI-7) every third day for 15 or 21 days.Results: The findings of this study showed that repeated bee venom treatment by sting or injection to adult male mice resulted in a significant decline in testosterone levels, sperm count, sperm motility, and a very significant increase in the percentage of abnormal sperm morphology; also, there were harmful testicular histological changes in the structural organization of seminiferous tubules and degenerative changes in the germinal epithelium compared to control group. Conclusion:The results of this study provide evidence for the low semen quality and adverse testicular histological changes in male mice treated with bee venom. Hence, there is a desperate need for educating alternative healthcare practitioners and infertile couples about the harmful effects of BVT on reproductive status.

The Effects of Folic Acid on Testicular Histology, Sperm Quality, and Spermatogenesis Indices Following 3,4-Methylenedioxymethamphetamine Exposure in Adult Male Rats.

BackgroundThere is an increasing concern over acute exposure of amphetamine and its derivative such as 3,4-methylenedioxymethamphetamine (MDMA) on male reproductive toxicity. Supplementary vitamins can reduce the oxidative stresses and repair the damages on reproductive organs. This experimental study was conducted to evaluate the effects of folic acid (FA) on reproductive indices, the antioxidant enzyme activities, and histological changes of testis on adult male rats treated by MDMA.MethodsThis experimental study was conducted on adult male Wistar rats. Animals were divided into 4 groups: control, MDMA, FA, and MDMA + FA. Animals received a dose of 10 mg/kg of MDMA and 1 mg/kg of FA for 7 or 14 days. Rats were anesthetized and sperm quality parameters (number, concentration, motility, and morphology), spermatogenesis indices [the mean seminiferous tubule diameter (MSTD), spermiogenesis index (SI), repopulation index (RI), and tubular differentiation index (TDI)], changes on testicular structure, antioxidant enzyme activities of catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) beside serum level of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone were measured. Data were analyzed, using the one-way analysis of variance (ANOVA) and SPSS software.FindingsMDMA (both 7 and 14 days) caused significant changes in sperm quality (P < 0.001), spermatogenesis indices (P < 0.001), testicular histopathology, and level of LH, FSH, testosterone beside the antioxidant enzyme activities (SOD, CAT, and MDA) (P < 0.001). Supplementation of FA in association with MDMA partially reversed these parameters and made them close to the control group.ConclusionThe results suggested that FA could reduce the adverse effect of MDMA on reproductive ability in adult male rats.

Effect of Introducing Foreign DNA into Nile Tilapia, Oreochromis Niloticus Gonads on Male Fertility of the Progeny Produced Under Salinity Stress

This work was conducted to study the effect of transfer foreign DNA isolated from sea bream, Sparus aurata or Artemia , Artemia salina into the ovaries and testes of O.niloticus adult on male fertility of the progeny produced under different salinity levels through studying the histological changes of testes. The results showed that male fertility of O. niloticus decreased as a result of introducing foreign DNA into the gonads of their parents. This decrease depends on the type of DNA since the results of the testes investigation revealed that O. niloticus received Artemia DNA displayed normal lobules of 40 %. In contrast, O. niloticus received sea bream DNA, showed only 28 % of normal lobules. The results also showed that the fertility of males decreased with increasing salinity levels up to 36 ppt. Although all fish received foreign DNA displayed low fertility, Nile tilapia received Artemia DNA showed more salinity tolerance and highest fertility than Nile tilapia received sea bream DNA.

Effects of Icariin on Histomorphometric Changes of Testis and Prostate Induced by Acrylamide in Mice

Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric changes of testis and prostate induced by Acrylamide. Materials and Methods: Male mice were divided into four groups (n=8): A is the control group and does not get any treatment, B is the sham group and only received drinking water. C group received Acrylamide 10 mg/kg. D group received Acrylamide 15 mg/kg+1.5 mg/kg of Icariin. Histological changes in testis and prostate were examined using stereological methods. Results: Results showed decreases in testis weight of the group treated by (p≤0.01) and the group cured by Acrylamide +Icariin group (p≤0.05). The total volume of testis showed a reduction in the Acrylamide group compared to other groups (p≤0.05). The total number of spermatogonia and spermatocyte cells in the Acrylamide group showed a decrease in comparison with the other groups (p≤0.05). The total number of spermatid cells in the Acrylamide group indicated a significant reduction in comparison with the control and sham group (p≤0.05). The total number of sertoli cells in the Acrylamide group showed a reduction when the number of leydig cells in the Acrylamide group showed a significant decrease in comparison with the control, sham, and Acrylamide+Icariin groups (p≤0.05). The mean Johnsen score was decreased in the Acrylamide treated group compared to control, sham, and Acrylamide+Icariin groups (p≤0.05). Testosterone concentration in the Acrylamide group showed a reduction in comparison with control, sham, and Acrylamide+Icariin groups (p≤0.05). Conclusion: Results demonstrated that Acrylamide altered the structure of the testis, prostate gland, and spermatogenesis stage, and Icariin treatment improved these histopathological changes.

The effects of maternal separation stress experienced by parents on male reproductive potential in the next generation

There is little information available about the effects of early-life parental stress on the reproductive potential of the next generation. The aim of this study is to examine the reproductive potential of male mice whose parents experienced maternal separation stress. In the present study, male first-generation offspring from parents were undergone of maternal separation (MS) were examined. Sperm characteristics, histological changes in testis, reactive oxygen species (ROS) production, expression of apoptotic and inflammatory genes and proteins were assessed. Findings showed that MS experienced by parents significantly decreased the morphology and viability of spermatozoa. Furthermore, significant changes in testicular tissue histology were observed. Increased production of ROS, decreased glutathione peroxidase (GPX) and adenosine triphosphate (ATP) concentrations, and affected the expression of genes and cytokines involved in inflammation. Finally, the mean percentage of caspase-1 and NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) positive cells was significantly higher in first-generation group. MS experienced by parents may negatively affect the reproduction of first generation offspring.

Molecular characterization of AwSox2 from bivalve Anodonta woodiana: Elucidating its player in the immune response.

Sox2 is an embryonal stem cell Ag essential for early embryonic development, tissue homeostasis and immune regulation. In the current study, one complete Sox2 cDNA sequence was cloned from freshwater bivalve Anodonta woodiana and named AwSox2. Histological changes of testis derived from Bisphenol A (BPA) treatment were analyzed by hematoxylin and eosin staining. Expressions of AwSox2 derived from BPA, LPS and polyinosinic:polycytidylic (Poly I:C) challenge were measured by quantitative real-time PCR. The full-length cDNA of AwSox2 contained an open reading frame of 927 nucleotides bearing the typical structural features of Sox2 family. Obvious degeneration, irregular arrangement of spermatids, and clotted dead and intertwined spermatids were observed in BPA-treated groups. Administration of BPA could result in a dose-dependent up-regulation of AwSox2 expression in the male gonadal tissue of A. woodiana. In addition, expression of AwSox2 was significantly induced by LPS and Poly I:C treatment in the hepatopancreas, gill and hemocytes, compared with that of control group. These results indicated that up-regulations of AwSOx2 are closely related to apoptosis of spermatogonial stem cells derived from BPA treatment as well as enhancement of immune defense against LPS and Poly I:C challenge in A. woodiana.

The effect of green tea extract on the sperm parameters and histological changes of testis in rats exposed to para-nonylphenol.

BackgroundPara-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals. Objective: The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats.Materials and Methods24 adult male Wistar rats (215 20 gr) were randomly divided into four groups (n = 6/each) – including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE – and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated.ResultsA significant decrease in the sperm number, motility, viability and morphology (p 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE.ConclusionIt seems GTE can be used as a potent antioxidant in the case of p-NP toxication.