The Chinese Space Station Combustion Science Experiment System is capable of conducting microgravity combustion experiments of multiple fuel types in orbit. The combustion chamber, one of the key components, is a pressure vessel that can be sealed. It is equipped with interfaces for the experiment inserts such as mechanical, oxidizer, exhaust gas emission, power supply, control and cooling. In this paper, the detailed design and analysis of the combustion chamber is based on the technical indicators such as pressure-bearing range, leakage rate, and transmitted light band. The combustion chamber adopts a sectional structure, which consists of a hinged door assembly, a locking ring assembly, a connecting ring assembly, a cylinder assembly, <italic>etc</italic>. Seals are used at the connection positions. The strength, stiffness and random vibration response of the combustion chamber structure were checked through pressure analysis, modal analysis and random response analysis. The results verified the safety and reliability of the combustion chamber design, which can meet launch and in-orbit operational requirements.