The Tulsi plant, also known as Holy Basil, is a sacred herb in Hinduism and is revered for its medicinal properties as well. It belongs to the Lamiaceae (mint) family and is native to the Indian subcontinent. Tulsi is widely cultivated and is found in many Hindu households where it is worshipped daily. In this work there was an attempt to made to find out chlorophyll content of different Tulsi species found in Nuapada district, Odisha. In this work we were compare chlorophyll pigment of 4 different species of tulsi of Nuapda such as Ocimum tenuiflorum (black tulshi), Ocimum sanctum (green tulshi), Ocimum basilcum (dohna) and Ocimum gratissuma (van tulshi). Among them maximum total chlorophyll content found in Ocimum tenuifflorum And list in Ocimum sanctum. The chlorophyll a found maximum in Ocimum basilcum. And minimum in Ocimum gratissuma.The chlorophyll b found maximum in Ocimum basilcum and minimum in Ocimum tenuiflorum. The total chlorophyll found Ocimum gratissum and minimum in Ocimum sanctum. Such type of work provides us data regarding distribution of chlorophyll in different species of a genera.