The amalgamation of Himalayan and Indo-Burmese biodiversity has made the state of Manipur, India, a unique ecosystem. In addition, the region is a strategic place for the country to establish an economic corridor to Southeast Asia. In recent times, the region has witnessed tremendous infrastructural/road development. Subsequently, forest fragmentation related to urbanization and road expansion has emerged in the Himalayan foothills. The development of roads brought rapid changes in land use and land cover (LULC) and thus subsequent environmental degradation. The current study attempts to understand how the development of road networks has impacted the natural cover in Manipur, India. A spatio-temporal analysis was performed to assess the relationship between the development of road networks and LULC changes using the Landsat satellite images over a decade (2012-2022). The results showed significant changes in the area coverage of LULC categories such as agricultural land, built-up areas, forest, and water bodies with the increase in road density. To have a holistic view, the study area was segregated into three functional zones based on their urban land use pattern, i.e., urban center, peri-urban, and urban peripheral fringes. Urban sprawl in the urban center has led to the rapid conversion of forested lands into built-up areas and agricultural lands in the peri-urban and urban peripheral fringes, respectively. The decline of forest areas to urbanization in peri-urban and urban peripheral fringes calls for conservation and restoration initiatives. The study also emphasizes how different stakeholders can be involved and empowered to strengthen public-private partnerships for conservation and restoration in such sensitive ecosystems. Urban planners and developers should be critical in making informed decisions through understanding ecological concerns in tandem with infrastructural development.