In order to solve the problems of large error and long time-consuming in the traditional mechanical property test methods, a new mechanical property test method for highway concrete pavement construction machinery materials is proposed. The relationship between the deformation component and the displacement component of the microstructure of the concrete pavement in the equilibrium coordinate system is obtained. The limit value of the critical thickness of the construction material of the highway concrete pavement is obtained by the second derivative function. Based on the analysis principle of AFM force curve, the relationship between mechanical materials and elastic modulus of highway concrete pavement construction is determined, and the performance analysis of highway concrete pavement construction mechanical materials is realised. The test results show that the error of mechanical properties analysis of highway concrete pavement construction machinery materials by the proposed method is only 0.3%, and the test time is short.