High Cost-effectiveness Research Articles

Aducanumab in Alzheimer's Disease: A Critical Update.

Alzheimer's disease (AD) is a complex neurological disorder that results in cognitive decline. The incidence rates of AD have been increasing, particularly among individuals 60 years of age or older. In June 2021, the US FDA approved aducanumab, the first humanized monoclonal antibody, as a potential therapeutic option for AD. Clinical trials have shown this drug to effectively target the accumulation of Aβ (beta-amyloid) plaques in the brain, and its effectiveness is dependent on the dosage and duration of treatment. Additionally, aducanumab has been associated with improvements in cognitive function. Biogen, the pharmaceutical company responsible for developing and marketing aducanumab, has positioned it as a potential breakthrough for treating cerebral damage in AD. However, the drug has raised concerns due to its high cost, limitations, and potential side effects. AD is a progressive neurological condition that affects memory, cognitive function, and behaviour. It significantly impacts the quality of life of patients and caregivers and strains healthcare systems. Ongoing research focuses on developing disease-modifying therapies that can halt or slow down AD progression. The pathogenesis of AD involves various molecular cascades and signaling pathways. However, the formation of extracellular amyloid plaques is considered a critical mechanism driving the development and progression of the disease. Aducanumab, as a monoclonal antibody, has shown promising results in inhibiting amyloid plaque formation, which is the primary pathological feature of AD. This review explores the signaling pathways and molecular mechanisms through which aducanumab effectively prevents disease pathogenesis in AD.

Ionic Covalent Organic Framework Membrane as Active Separator for Highly Reversible Zinc-Sulfur Battery.

Zinc-sulfur (Zn-S) batteries exhibit a high theoretical energy density, nontoxicity, and cost-effectiveness, demonstrating significant potential for integration into large-scale energy storage systems. However, the phenomenon of polysulfide (including dissolved S8 and Sx2-) shuttling is a major issue that results in rapid capacity decay and a short lifespan, limiting the practical performance of sulfur-based batteries. Herein, we fabricated an ionic covalent organic framework (iCOF) membrane as an active separator for the Zn-S battery. Sulfonic acid groups were introduced to the COF membrane, providing abundant negative charge sites in its pore wall. By combining size sieving and charge interaction between the polysulfide and pore wall, the iCOF membrane inhibited the crossover of polysulfides to the Zn metal anode without affecting the transport of metal ions. The Zn-S battery with the iCOF membrane as the separator shows a high-performance and low attenuation rate of 0.05% per cycle over 300 cycles at 2.5 A g-1. This study emphasizes the significance of separator design in enhancing Zn-S batteries and showcases the potential of functionalized framework materials for the development of high-performance energy storage systems.

Molten Salt-Assisted Synthesis of Catalysts for Energy Conversion.

A breakthrough in manufacturing procedures often enables people to obtain the desired functional materials. For the field of energy conversion, designing and constructing catalysts with high cost-effectiveness is urgently needed for commercial requirements. Herein, the molten salt-assisted synthesis (MSAS) strategy is emphasized, which combines the advantages of traditional solid and liquid phase synthesis of catalysts. It not only provides sufficient kinetic accessibility, but effectively controls the size, morphology, and crystal plane features of the product, thus possessing promising application prospects. Specifically, the selection and role of the molten salt system, as well as the mechanism of molten salt assistance are analyzed in depth. Then, the creation of the catalyst by the MSAS and the electrochemical energy conversion related application are introduced in detail. Finally, the key problems and countermeasures faced in breakthroughs are discussed and look forward to the future. Undoubtedly, this systematical review and insights here will promote the comprehensive understanding of the MSAS and further stimulate the generation of new and high efficiency catalysts.

Molecular Insights into the Anticancer Activity of Withaferin-A: The Inhibition of Survivin Signaling.

Survivin, a member of the IAP family, functions as a homodimer and inhibits caspases, the key enzymes involved in apoptosis. Several Survivin inhibitors, including YM-155, Debio1143, EM1421, LQZ-7I, and TL32711, have emerged as potential anticancer drugs awaiting validation in clinical trials. Due to the high cost and adverse side effects of synthetic drugs, natural compounds with similar activity have also been in demand. In this study, we conducted molecular docking assays to evaluate the ability of Wi-A and Wi-N to block Survivin dimerization. We found that Wi-A, but not Wi-N, can bind to and prevent the homodimerization of Survivin, similar to YM-155. Therefore, we prepared a Wi-A-rich extract from Ashwagandha leaves (Wi-AREAL). Experimental analyses of human cervical carcinoma cells (HeLa and ME-180) treated with Wi-AREAL (0.05-0.1%) included assessments of viability, apoptosis, cell cycle, migration, invasion, and the expression levels (mRNA and protein) of molecular markers associated with these phenotypes. We found that Wi-AREAL led to growth arrest mediated by the upregulation of p21WAF1 and the downregulation of several proteins (CDK1, Cyclin B, pRb) involved in cell cycle progression. Furthermore, Wi-AREAL treatment activated apoptosis signaling, as evidenced by reduced PARP-1 and Bcl-2 levels, increased procaspase-3, and elevated Cytochrome C. Additionally, treating cells with a nontoxic low concentration (0.01%) of Wi-AREAL inhibited migration and invasion, as well as EMT (epithelial-mesenchymal transition) signaling. By combining computational and experimental approaches, we demonstrate the potential of Wi-A and Wi-AREAL as natural inhibitors of Survivin, which may be helpful in cancer treatment.

Frictional pressure drop of the vertically upward gas–liquid two-phase flow in an airlift pump system

Airlift pumps (ALPs) are promising in the oil and chemical industry, owing to their advantages such as a simple structure, convenient operation, wide applicability, high cost-effectiveness, environmental friendliness, safety, and reliability. However, there are few studies on the frictional pressure drop of vertically upward gas–liquid two-phase flow in ALPs. Therefore, this study presents an experimental investigation of the frictional pressure drop in the vertically upward gas–liquid two-phase flow in ALPs. Experiments were conducted in a vertical pipe with a total length of 3.245 m and a two-phase section of 2.8 m; the working pressure of the air compressor was 0.4 MPa, pipe diameter was 0.05 m, submergence ratio ranged from 0.6 to 0.85, and gas superficial velocity ranged from 0 to 4 m/s. A total of 74 sets of experimental data were obtained, and the frictional pressure drop models of 36 classical gas–liquid two-phase flows were evaluated. The results indicated that classical gas–liquid two-phase flow models significantly underestimated the experimental results. By analyzing the experimental data, visualizing the internal flow field, and performing theoretical derivations, a new frictional pressure drop correlation was established for the vertically upward gas–liquid two-phase flow in ALPs. The results demonstrated that the new model could accurately predict the frictional pressure drop of ALPs with mean percentage error, mean absolute percentage error, and root mean square percentage error values of 7.8%, 12.18%, and 25.86%, respectively.

Unified Crystal Phase Control with MACl for Inducing Single-Crystal-Like Perovskite Thin Films in High-Pressure Fusion Toward High Efficiency Perovskite Solar Cell Modules.

Perovskite solar cells, recognized for their high photovoltaic conversion efficiency (PCE), cost-effectiveness, and simple fabrication, face challenges in PCE improvement due to structural defects in polycrystalline films. This study introduces a novel fabrication method for perovskite films using methylammonium chloride (MACl) to align grain orientation uniformly, followed by a high-pressure process to merge these grains into a texture resembling single-crystal perovskite. Employing advanced visual fluorescence microscopy, charge dynamics in these films are analyzed, uncovering the significant impact of grain boundaries on photo-generated charge transport within perovskite crystals. A key discovery is that optimal charge transport efficiency and speed occur in grain centers when the grain size exceeds 10µm, challenging the traditional view that efficiency peaks when grain size surpasses film thickness to form a monolayer. Additionally, the presence of large-sized grains enhances ion activation energy, reducing ion migration under light and improving resistance to photo-induced degradation. In application, a perovskite solar cell module with large grains achieve a PCE of 22.45%, maintaining performance with no significant degradation under continuous white LED light at 100 mA cm-2 for over 1000h. This study offers a new approach to perovskite film fabrication and insights into optimizing perovskite solar cell modules.

Nafion/ZrO2 Modified NiTi Orthodontic Wire: Preparation, Material Characterization, and Corrosion Studies

Background: Nickel-titanium (NiTi) orthodontic wires are widely used in dental corrective procedures due to their high mechanical properties and cost-effectiveness. However, they are prone to oral corrosion, leading to mechanical deterioration, aesthetic issues, and potential health concerns. Objective: This study aims to improve the corrosion resistance and durability of NiTi orthodontic wires by employing zirconium dioxide (ZrO2) and Nafion coating with the goal of enhancing wire performance. Methods: Two types of NiTi wires were evaluated: a standard, unmodified wire as a control and another wire treated with electrodeposited ZrO2 film and Nafion (Naf) coating. Surface analysis was conducted using various techniques, including Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray Spectroscopy (EDX) analysis. Results: The uncoated NiTi wire exhibited a corrosion rate of 4.436× 10-1 mm/year, whereas the Naf/ZrO2-coated NiTi wire showed a corrosion rate of 4.068× 10-1 mm/year, indicating that the Naf/ZrO2 coating acted as a protective layer. Additionally, the ZrO2 layer provided poor electrical conductivity, resulting in slightly higher impedance compared to bare NiTi. The coating served as a barrier, which significantly enhanced corrosion resistance and improved the wire lifespan. Conclusion: Electro-modification through ZrO2 deposition and Nafion coating significantly improved the corrosion resistance and overall durability of NiTi orthodontic wires, offering a promising advancement for their use in dental orthodontics. This study underscores the potential of ZrO2 and Nafion coating to enhance the corrosion resistance and longevity of NiTi orthodontic wires.

Ultralong lifespan solid-state sodium battery with a supersodiophilic and fast ionic conductive composite sodium anode

Solid-state sodium batteries (SSNBs) are considered as a promising alternative to organic liquid-based batteries due to their excellent safety, high energy density and cost-effectiveness. However, SSNBs suffer from undesirable interfacial contact between Na and solid-state electrolytes such as NASICON-type Na3Zr2Si2PO12 (NZSP), dendritic growth and dramatic volume changes during cycling, which hinder their development towards actual applications. Herein, dendrite-free composite-type Na/NZSP module with ultrafast built-in ionic conductive framework is designed to promote the Na diffusion kinetics, partially restrict the volume effect, and simultaneously improve the wettability towards NZSP. Thanks to the unique module with supersodiophilic property, the thus-made all-solid-state Na-symmetric cells offer a reduced area specific resistance by more than two orders of magnitudes (from 1774.0 Ω cm2 for the pristine Na/NZSP to 14.1 Ω cm2 for the composite-type Na/NZSP module) and endow an ultralong lifespan of 7800 h at room temperature. Moreover, a full SSNB coupling with the Na/NZSP module and Na3V2(PO4)3 cathode achieves extremely long and stable cycling of more than 5760 cycles at 1.0 C with 87.9 % of capacity retention and high-rate capability at 3.0 C, being among the best achievements reported so far. The findings open a new window of composite-type Na/NZSP module design for high-performance SSNBs.

Development of a wireless multichannel miniature impedance measurement system and its application for bolt loosening detection

The piezoelectric impedance-based technique is always regarded as one of the most promising structural health monitoring and nondestructive evaluation methods. In recent years, impedance measurement chip AD5933 with the characteristics of high integration and cost-effectiveness makes it possible to address the huge and high-cost problems of the commercialized impedance measurement instrument during the process of structural health monitoring and defect identification. However, it still faces lots of challenges for the chips to be utilized in practical applications due to several limitations, such as short distance for data transmission, single measurement channel and artificial attendant requirement. In this paper, a wireless multichannel miniature impedance measurement system composed by the front-end measurement device and the remote measurement and control platform on the server, is firstly developed and presented with the functions of wireless data transmission, multi-channel acquisition and remote data post-processing. Subsequently, the design concept and composition of the system were introduced in detail. Then, a series of piezoelectric transducers related tests were conducted to validate its impedance measurement performance, especially when comparing with the ones measured by commercialized instruments. In addition, to verify its effectiveness and feasibility of the developed system for the structural damage detection, a bolt loosening detection experiment on the flange connection of a pipeline specimen was investigated for its damage localization and severity quantification. Finally, all the results demonstrated that the developed system provides a great possibility to be used as a convenient and portable impedance measurement tool for the civil structural health monitoring and damage identification in practical applications.

Corrosion-Resistant Ultrathin Cu Film Deposited on N-Doped Amorphous Carbon Film Substrate and Its Use for Crumpleable Circuit Board.

Copper (Cu) is widely used as an industrial electrode due to its high electrical conductivity, mechanical properties, and cost-effectiveness. However, Cu is susceptible to corrosion, which degrades device performance over time. Although various methods (alloying, physical passivation, surface treatment, etc.) are introduced to address the corrosion issue, they can cause decreased conductivity or vertical insulation. Here, using the nitrogen-doped amorphous carbon (a-C:N) thin film is proposed as a substrate on which Cu is directly deposited. This simple method significantly inhibits corrosion of ultrathin Cu (<20nm) films in humid conditions, enabling the fabrication of ultrathin electronic circuit boards without corrosion under ambient conditions. This study investigates the origin of corrosion resistance through comprehensive microscopic/spectroscopic characterizations and density-functional theory (DFT) calculations: i) diffusion of Cu atoms into the a-C:N driven by stable C-Cu-N bond formation, ii) diffusion of N atoms from the a-C:N to the Cu layer heading the top surface, which is the thermodynamically preferred location for N, and iii) the doped N atoms in Cu layer suppress the inclusion of O into the Cu lattice. By leveraging the ultrathinness and deformability of the circuit board, a transparent electrode and a crumpleable LED lighting device are demonstrated.

Aqueous benzene, toluene, ethylbenzene and xylene (BTEX) removal using core-shell structure activated carbon ball as a permeable reactive barrier material

Permeable reactive barriers (PRBs) are passive and sustainable treatment systems for remediating the diffusion of contaminant plumes in groundwater. Several conventional reactive materials such as activated carbon (AC) have long been used as reactive media for PRBs. AC, which is known for its high adsorption capability and cost-effectiveness, is commonly used to remove multiple pollutants from groundwater. Unfortunately, among the reactive materials, AC can fill in the barrier and pose practical problems, such as a pressure drop, solid losses during handling, and safe disposal of filled sorbents, because of its low particle strength. In this study, AC balls were prepared using zeolite as the core and powdered AC, quartz, and calcite as the shell. AC ball with excellent mechanical strength and high permeability properties in the form of a core–shell layer is a good alternative to conventional reactive materials. The adsorption characteristics of benzene, toluene, ethylbenzene, and p-xylene (BTEX) from solutions using AC balls were investigated. The adsorption equilibrium is in the order of X > E > T > B. The adsorption capacity for B (4.90 mg/g), T (3.37 mg/g), E (1.16 mg/g) and X (0.46 mg/g) were observed at solution pH = 7. To validate the proposed models, batch experiments indicated that the pseudo-2nd-order (11.61 and 10.54 mg/g) and Langmuir models (8.81 and 5.27 mg/g) were the most suitable for describing the kinetics and equilibrium of benzene and toluene, respectively. Regeneration experiments were performed using chemical extraction (methanol) and microwave (MW) heating. When the AC ball was reused six times, the removal efficiency for benzene was maintained above 81 % using methanol, whereas that for benzene was increased to 91 % using MW. A series of experiments revealed that AC balls have considerable reusability. The AC ball can be effectively used as a medium for the removal of benzene in a continuous flow system such as column and sandbox. Based on these results, AC balls are a potential reactive medium for field-scale PRB practical remediation applications.

Neonatal screening for critical congenital heart diseases in Peru: an urgent call

Congenital heart diseases are the most common congenital malformations worldwide and represent one of the leading causes of neonatal death, in addition to the significant use of human and financial resources by health systems. The purpose of this document is to support the implementation of neonatal screening for critical congenital heart diseases using pulse oximetry according to the different geographical altitudes of Peru. This technology is widely used worldwide and has high sensitivity, specificity, and cost-effectiveness. At many latitudes, it has led to better survival in this group of patients and in the neonatal population in general since its use in the early detection of sepsis, pneumonia, and other conditions that affect the oxygenation of the newborn. Neonatal screening for critical congenital heart disease is applicable at all levels of healthcare at a national level, and its implementation must be a priority to improve neonatal health.

Research Progress on the Microstructure Evolution Mechanisms of Al-Mg Alloys by Severe Plastic Deformation.

Al-Mg alloys are widely used as important engineering structural materials in aerospace engineering, transportation systems, and structural constructions due to their low density, high specific strength, corrosion resistance, welding capability, fatigue strength, and cost-effectiveness. However, the conventional Al-Mg alloys can no longer fully satisfy the demands of practical production due to difficulties caused by many defects. The high strength of Al-Mg alloys as non-heat treatment precipitation-strengthened alloys is achieved primarily by solid solution strengthening along with work hardening rather than precipitation strengthening. Therefore, severe plastic deformation (SPD) techniques can be often used to produce ultrafine-grained structures to fabricate ultra-high strength aluminum alloys. However, this approach often achieves the strengthening of material at the cost of reduced ductility. This paper comprehensively summarizes the various approaches of ultrafine/nanocrystalline materials for enhancing their plasticity, elaborates on the creation of a bimodal microstructure within the alloy, and discusses the formation of a nanotwin microstructure within the alloy and the incorporation of dispersed nanoparticles. The mechanisms underlying both the strengthening and toughening during large plastic deformation in aluminum alloys are summarized, and the future research direction of high-performance ultrafine crystalline and nanocrystalline Al-Mg aluminum alloys is prospected.

One-Step fabrication of bioinspired Peptide-Functionalized ice surface for bioanalysis

Solid surfaces modified with functional biomolecules that can selectively capture specific molecules play a crucial role in physical science and biomedical research. However, convenient biomolecule-modified materials are still faced with problems such as harsh synthesis conditions and time-consuming functionalization processes. As an easy-to-prepare solid substance, ice, holds the potential to be developed as an excellent functional material, but its inert nature hampers efficient functionalization with biomolecules. In this study, drawing inspiration from anti-freeze organisms in nature, we endow ice with biorecognition ability by leveraging the natural non-covalent binding between peptides and ice. By exploiting ice as a substrate modified with functional biomolecules, we demonstrate advantages such as high accessibility, one-step modification, and cost-effectiveness compared to conventional biomolecule-modified materials. Additionally, the temperature-controlled melting of ice offers a facile way to release captured biomolecules while maintaining their activity for further studies. The feature is further applied in protein assays, exhibiting satisfactory selectivity and quantitative capabilities with a minimum detectable concentration of approximately 0.1 ng/mL. Overall, our strategy to endow ice with desired biological functions provides a new tool for investigation in physical science and broader applications in the field of biomedicine.

Cable Eccentricity Detection Method Based on Magnetic Field.

Amid the rapid advancement of electronic information technology, the need for cable eccentricity measurement in the industry is increasing both in China and across the globe. Current detection methods have several flaws, including high costs, insufficient accuracy, and instability. In this paper, we introduce a magnetic field-based detection method for cable eccentricity that provides high precision and cost-effectiveness. We position three pairs of magnetic field-collection modules in a circular array to gather magnetic flux density information induced by the electrified cable. We apply the law of electromagnetic induction to calculate the cable eccentricity. Our method is non-contact, preserving the cable's integrity. Our method outperforms traditional detection methods, not only in achieving greater accuracy and stability but also in significantly lowering the detection cost. Simulations and experiments show that our method's error rate under specified conditions is 0~4%, with a maximum standard deviation of 0.11, confirming its precision and stability in detecting cable eccentricity. The effectiveness of our method is influenced by two factors: lift-off value and loading current intensity. Our method presents a novel concept and a dependable strategy for the progress of cable eccentricity-detection technology.

Sustained estradiol valerate delivery by microneedles: A new effective platform to construct estrogen-dependent tumor model

The establishment of an estrogen receptor-positive (ER+) breast tumor model plays a crucial role in the preliminary screening of anticancer drugs and understanding disease mechanism. However, the construction of this model requires exogenous estrogen supplementation. The current method of estrogen supplementation was limited by its high cost and potential side effects. Herein, we utilized Poly D, L-lactic-co-glycolic acid (PLGA) as substrate to develop the estradiol valerate microneedle patch (EVMP). The EVMP exhibited excellent drug loading capacity (46 μg/needle) and good mechanical strength (>0.2 N/needle). Importantly, the EVMP exhibited favorable sustained release of estradiol valerate ability in vitro. Subsequently, the EVMP successfully achieved sustained release in Balb/c nude mice, resulting in an average MCF-7 (ER+) tumor volume of approximately 300 mm3 at day 35. In addition, no significant inflammatory reaction or urinary calculi were observed during this process, which further demonstrated the efficacy and safety of the EVMP. Overall, these findings suggested that the EVMP held great potential as a safe and animal-friendly alternative platform for constructing the ER + breast cancer model.

Formation of hollow stepped FePBA@rGO anode for high-performance lithium-ion batteries

Prussian blue and its analogues (PBAs) have been considered promising anode materials for lithium-ion batteries (LIBs) owing to their high theoretical capacity and cost-effectiveness. However, they often encounter challenges such as poor electronic conductivity and structural instability. Herein, we introduce a hollow stepped FePBA framework structure coated with reduced graphene oxide (FePBA@rGO). This sophisticated design significantly improves electronic conductivity and optimizes the diffusion pathways for lithium ions, leading to exceptional cycling stability and rate performance. The FePBA@rGO-15 anode developed in this study shows a notable rate capability of 294 mAh g−1 at a current density of 2 A g−1. More significantly, it demonstrates exceptional lithium storage performance, maintaining a capacity of 1245 mAh g−1 after 600 cycles at a current density of 1 A g−1. Density functional theory simulations further support our findings by illustrating that the robust interface between FePBA and rGO significantly accelerates the reactions kinetics of Li+ within the FePBA framework. This study represents a significant advancement towards improving the capacity and cycling stability of PBA-based anode materials, offering promising prospects for future battery applications.

Investigating the Electrochemical Performance of MnFe2O4@xC Nanocomposites as Anode Materials for Sodium-Ion Batteries.

Transition metal oxides (TMOs) are important anode materials in sodium-ion batteries (SIBs) due to their high theoretical capacities, abundant resources, and cost-effectiveness. However, issues such as the low conductivity and large volume variation of TMO bulk materials during the cycling process result in poor electrochemical performance. Nanosizing and compositing with carbon materials are two effective strategies to overcome these issues. In this study, spherical MnFe2O4@xC nanocomposites composed of MnFe2O4 inner cores and tunable carbon shell thicknesses were successfully prepared and utilized as anode materials for SIBs. It was found that the property of the carbon shell plays a crucial role in tuning the electrochemical performance of MnFe2O4@xC nanocomposites and an appropriate carbon shell thickness (content) leads to the optimal battery performance. Thus, compared to MnFe2O4@1C and MnFe2O4@8C, MnFe2O4@4C nanocomposite exhibits optimal electrochemical performance by releasing a reversible specific capacity of around 308 mAh·g-1 at 0.1 A·g-1 with 93% capacity retention after 100 cycles, 250 mAh·g-1 at 1.0 A g-1 with 73% capacity retention after 300 cycles in a half cell, and around 111 mAh·g-1 at 1.0 C when coupled with a Na3V2(PO4)3 (NVP) cathode in a full SIB cell.

Enhancing cassava beer quality: Extrusion-induced modification of cassava starch structure boosts fermentable sugar content in wort

The high starch content and cost-effectiveness of cassava make it an attractive adjunct in beer brewing, with the fine structure of starch playing a crucial role in determining the composition of fermentable sugars (FS) and overall beer quality. This study investigated the effect of extrusion-induced changes in the starch structure of cassava flour on the FS profile of the wort and, consequently, on the quality attributes of cassava beer. The findings revealed that the shear stress during extrusion significantly reduced the molecular weight to 1.20 × 105g/mol and the branching degree of amylopectin. Simultaneously, there was an increase in the concentrations of short- and intermediate- chain amylose by 5.61% and 42.72%, respectively. These structural changes enhanced the enzymatic hydrolysis of extruded cassava flour (ECF), resulting in a higher total fermentable sugars content (22.00g/100 mL) in the ECF wort, predominantly composed of maltose and glucose. Furthermore, the altered FS profile led to an increased production of higher alcohols and esters in extruded cassava beer (ECB), particularly noted for the elevation of 2-phenylethyl alcohol levels, which imparted a distinctive rose aroma to the ECB. Consequently, the sensory profile of ECB showed significant improvement. This study offers critical insight into optimizing cassava beer quality and broadens the potential applications of cassava flour in the brewing industry.

Targeted DNA-seq and RNA-seq of Reference Samples with Short-read and Long-read Sequencing

Next-generation sequencing (NGS) has revolutionized genomic research by enabling high-throughput, cost-effective genome and transcriptome sequencing accelerating personalized medicine for complex diseases, including cancer. Whole genome/transcriptome sequencing (WGS/WTS) provides comprehensive insights, while targeted sequencing is more cost-effective and sensitive. In comparison to short-read sequencing, which still dominates the field due to high speed and cost-effectiveness, long-read sequencing can overcome alignment limitations and better discriminate similar sequences from alternative transcripts or repetitive regions. Hybrid sequencing combines the best strengths of different technologies for a more comprehensive view of genomic/transcriptomic variations. Understanding each technology’s strengths and limitations is critical for translating cutting-edge technologies into clinical applications. In this study, we sequenced DNA and RNA libraries of reference samples using various targeted DNA and RNA panels and the whole transcriptome on both short-read and long-read platforms. This study design enables a comprehensive analysis of sequencing technologies, targeting protocols, and library preparation methods. Our expanded profiling landscape establishes a reference point for assessing current sequencing technologies, facilitating informed decision-making in genomic research and precision medicine.