High-value Utilization Research Articles

Rheological behavior and microscopic characteristic of electromagnetic thermal activated crumb rubber and SBS modified asphalt

Rubber activation provides a feasible approach for the preparation of high-content rubber modified asphalt without increasing the complexity of the production process. The electromagnetic thermal activation method was used for crumb rubber. The high dosage (30 % mass ratio of neat asphalt) activated crumb rubber and SBS (1.2 % mass ratio of neat asphalt) modified asphalt (ARMA) was prepared. The neat asphalt (NA) and 20 % non-activated crumb rubber modified asphalt (RMA) constituted the control group. Storage stability, rheological behavior, and microscopic properties tests were conducted to evaluate the performance differences of them. The rheological behavior of RMA and ARMA was superior to NA. The electromagnetic thermal activation method fragmented rubber particles into numerous disordered small particles, thereby expanded the contact area with asphalt. This resulted in the storage stability of ARMA significantly better than that of RMA. The cross-linked wrinkles were generated by the combined effect of SBS modifier and activated crumb rubber, which enhanced the rheological, anti-fatigue, and elastic behavior. Both RMA and ARMA exhibited high-temperature PG grades of 82˚C. At 70°C, ARMA retained the capability to endure Extremely Heavy Traffic Grade conditions. At a strain level of 2.5 %, ARMA exhibited a 178 % increase in fatigue life compared to RMA. The crumb rubber was dispersed within the RMA and ARMA. Both ARMA and RMA did not exhibit the obviously bee-structure. However, ARMA exhibited a stronger polymer network. Moreover, both the carbonyl index and sulfoxide index of ARMA were lower than those of RMA and NA. The activation process improved the compatibility between rubber and asphalt, and realized the high value utilization of waste rubber.

Study on the Function of SlWRKY80 in Tomato Defense against Meloidogyne incognita.

WRKY transcription factors (TFs) can participate in plant biological stress responses and play important roles. SlWRKY80 was found to be differentially expressed in the Mi-1- and Mi-3-resistant tomato lines by RNA-seq and may serve as a key node for disease resistance regulation. This study used RNAi to determine whether SlWRKY80 silencing could influence the sensitivity of 'M82' (mi-1/mi-1)-susceptible lines to M. incognita. Further overexpression of this gene revealed a significant increase in tomato disease resistance, ranging from highly susceptible to susceptible, combined with the identification of growth (plant height, stem diameter, and leaf area) and physiological (soluble sugars and proteins; root activity) indicators, clarifying the role of SlWRKY80 as a positive regulatory factor in tomato defense against M. incognita. Based on this phenomenon, a preliminary exploration of its metabolic signals revealed that SlWRKY80 stimulates different degrees of signaling, such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH), and may synergistically regulate reactive oxygen species (ROS) accumulation and scavenging enzyme activity, hindering the formation of feeding sites and ultimately leading to the reduction of root gall growth. To our knowledge, SlWRKY80 has an extremely high utilization value for improving tomato resistance to root-knot nematodes and breeding.

Oriented construction of lignin-derived carbon hybrid electrocatalyst with superwettable surface and Co-based heterostructure for overall water splitting

Converting lignin waste into carbon hybrid catalysts for water electrolysis engenders a beneficial scenario for both the valorization of waste carbon resources and the reduction of hydrogen production costs, which remains a grand challenge. Hence, we report a novel lignin-derived carbon hybrid electrocatalyst as a low-cost and highly efficient bifunctional electrocatalyst for overall water splitting, prepared by a feasible strategy involving NaCl-assisted pyrolysis and subsequent oxidation-phosphorization. Notably, this strategy can not only regulate the composition of CoP/CoOx nano-heterostructures but also improve the structural properties of porous carbon matrix. Profiting from superwettable surface, ultrafine Co-based heterojunctions and large active area, the optimal CoP/CoOx-HPC catalyst exhibits exceptional bifunctional catalytic activity in 1 M KOH electrolyte, requiring only small overpotentials of 396 and 522 mV for HER and OER, respectively, to achieve a large current density of 400 mA cm−2. Meanwhile, the assembled alkali-electrolyzer using CoP/CoOx-HPC as both cathode and anode needs a relatively low voltage of 2.31 V at 400 mA cm−2, significantly outperforming the commercial couple of Pt/C || RuO2. This work can be of value in the design of the low-cost and highly active lignin-derived electrocatalysts and also serve as guidance for the high-value utilization of lignin or even other biomass wastes.

A cellulose-based light-management film incorporated with benzoxazine resin/tannic acid exhibiting UV/blue light double blocking and enhanced mechanical property

Cellulose, as a biomass resource, has attracted increasingly attention and extensive research by virtue of its widely sources, ideal degradability, good mechanical properties and easy modification due to its rich hydroxyl groups. Nevertheless, it is still a challenge to attain high performance cellulose-based composite film materials with diverse functional combinations. In this work, we developed a multifunctional cellulose-based film via a facile impregnation-curing strategy. Here, benzoxazine resin (BR) is used as an optically functional component to endow the microfibrillated cellulose (MFC) film with powerful light management capabilities including UV and blue light double shielding, high transmittance, and high haze. Meanwhile, the introduction of tannic acid (TA) substantially enhanced the mechanical properties of the film, including tensile strength and toughness, by constructing energy-sacrificial bonds. An effective self-healing of the film was achieved by controlling the degree of BR curing. The final films exhibited 98.24 % UV shielding and 89.98 % blue light blocking, good mechanical properties including a tensile strength of 202.21 MPa and tensile strain of 7.1 %, as well as desirable thermal healing properties supported by incompletely cured BR. This work may provide new insights into the high-value utilization of biomass resources.

Effect of solid-state co-fermentation on the structural characteristics and bioactivities of polysaccharides in the medicinal residues of GastrodiaGanoderma

To investigate the effect of solid-state co-fermentation on the structural characteristics and bioactivities of polysaccharides in the medicinal residues of Gastrodia Ganoderma (RGG), two kinds of polysaccharides were isolated and purified from unfermented RGG and fermented RGG, named RP and FRP, respectively. The results showed that molecular weight of FRP decreased than RP and its monosaccharide composition changed significantly. RP was mainly composed of Glucose (66.46%) and Galactose (22.83%) while the main monosaccharide components of FRP was galacturonic acid (65.67%). Fermentation caused the surface morphology of FRP to become fragmented and loose, and increased its in vitro antioxidant activity. Both RP and FRP could repair cell damage, reduce the secretion of reactive oxygen species, and protect RAW 264.7 cells from LPS-induced inflammation. Molecular docking results indicated that monosaccharide structures in FRP had stronger binding energy for inflammatory markers. This study may provide theoretical reference for the high-value utilization of Chinese herbal residues.

Sustainability of biomass pyrolysis for bio-aromatics and bio-phenols production: Life cycle assessment of stepwise catalytic approach

The high-valued utilization of biomass resources remains challenging due to their complex biochemical compositions and limited product selectivity. Herein, we propose a novel biomass stepwise catalytic pyrolysis process to efficiently produce aromatic hydrocarbons and phenols by leveraging the distinct thermal stability of biomass components and their compatibility with ZSM-5 and biochar-based catalysts. A life cycle assessment (LCA) is conducted to evaluate the energy consumption and environmental impact of this innovative process compared to conventional methods. Our findings reveal that this approach significantly reduces the energy consumption by 51.76–90.02 % across different application scenarios of by-product biochar. Additionally, using biochar as a soil amendment contributes to carbon negativity, achieving a net greenhouse gas (GHG) emission reduction of up to 6 t CO2 eq for producing 8.325 t aromatics and 1 t phenol. The first-stage catalytic pyrolysis for producing aromatic hydrocarbons is identified as the major contributor to environmental impact, mainly due to ZSM-5 catalyst usage and loss, while the second stage for phenol production has a comparatively minor impact. Finally, sensitivity analysis of the key parameters highlights areas for process optimization, including reducing catalyst dosage, minimizing energy consumption, and improving phenol yield, which are crucial for enhancing the environmental and economic viability of the entire route. This study provides a detailed LCA and offers insights for future improvements in biomass conversion technology.

Sea Cucumber Viscera Processed by Protease Hydrolysis Combined with Cordyceps militaris Fermentation Protect Caco-2 Cells against Oxidative Damage via Enhancing Antioxidant Capacity, Activating Nrf2/HO-1 Pathway and Improving Cell Metabolism.

Excessive reactive oxygen species (ROS) may lead to oxidative damage and metabolic disorder. The pathogenesis of human bowel inflammation is closely related to oxidative damage of intestinal epithelial cells caused by ROS. This study aimed to explore the high-value utilization of the byproducts of sea cucumber in antioxidant food for colitis prevention. The technology of protease hydrolysis combined with Cordyceps militaris fermentation was used to obtain fermented sea cucumber viscera protease hydrolysates (FSVHs). The results revealed that FSVH could enhance antioxidant capacity and alleviate oxidative damage and apoptosis by activating the Nrf2/HO-1 pathway and triggering the self-protection immune mechanisms. Moreover, the FSVH supplementation could upregulate antioxidant-related metabolic pathways of Caco-2 cells such as glutathione metabolism, confirming the enhanced antioxidant capacity of damaged cells. In summary, FSVH could exert protective effects on Caco-2 cells in response to oxidative damage, providing a promising prospect for sea cucumber resource utilization and colitis prevention.

Lignin-based vitrimer containing dynamic borate ester bonds with intrinsic photoconversion and excellent photothermal remoldability

The development of photoresponsive shape memory materials based on the photothermal conversion properties of lignin and the low activation energy of the dynamic covalent borate bond is of great importance. In this paper, a kind of lignin-based vitrimer polymer (LBP) containing dynamic boronic ester bonds was prepared by a “sulfhydryl-epoxy” click reaction and etherification reaction. The results show that the rigid segment EP-EL (lignin-based epoxy resin) and BDB (2,2′-(1,4-phenylene)-bis-[4-mercapto-1,3,2-dioxaneborane]) with benzene ring structure can impart tensile strength (20.8 MPa) to the LBP, while the flexible segment PEG imparts good elongation at break (15 %). The dynamic binding and dissociation exchange mechanism of the boronic ester bonds enables LBP to exhibit thermal remodelling properties (up to 36.2 %) and water-assisted self-healing properties at room temperature (up to 49.0 %). In addition, LBP exhibits excellent thermal and light-responsive shape memory properties due to its own photothermal conversion performance (photothermal conversion efficiency up to 18.2 %) and the dynamic boronic ester bond thermal activation bond exchange mechanism. The insulating properties of LBP make it suitable for use in high temperature protection circuit devices and light-responsive circuit devices. This study provides new insights into the design and application of Vitrimer and photoresponsive shape memory polymers, and also offers a new avenue for high-value utilization of lignin.

Al Impurity Upcycled High-Voltage Cathodes from Spent LiCoO2 Batteries.

Al impurity is among the most likely components to enter the spent lithium-ion battery (LIB) cathode powder due to the strong adhesion between the cathode material and the Al current collector. However, high-value metal elements tend to be lost during the deep removal of Al impurities to obtain high-purity metal salt products in the conventional hydrometallurgical process. In this work, the harmful Al impurity is designed as a beneficial ingredient to upcycle high-voltage LiCoO2 by incorporating robust Al-O covalent bonds into the bulk of the cathode assisted with Ti modification. Benefiting from the strong Al-O and Ti-O bonds in the bulk, the irreversible phase transitions of the upcycled R-LCO-AT have been significantly suppressed at high voltages, as revealed by in situ XRD. Moreover, a Li+-conductive Li2TiO3 protective layer is constructed on the surface of R-LCO-AT by pinning slow-diffusion Ti on the grain boundaries, resulting in improved Li+ diffusion kinetics and restrained interface side reactions. Consequently, the cycle stability and rate performance of R-LCO-AT were significantly enhanced at a high cutoff voltage of 4.6 V, with a discharge capacity of 189.5 mAhg-1 at 1 C and capacity retention of 92.9% over 100 cycles at 4.6 V. This study utilizes the detrimental impurity element to upcycle high-voltage LCO cathodes through an elaborate bulk/surface structural design, offering a strategy for the high-value utilization of spent LIBs.

Fabrication of high performance poly(vinyl alcohol)/straw composite film used for package via melt casting and biaxial stretching

A high performance poly(vinyl alcohol)/straw (PVA/SP) composite film for package was fabricated in this study by using thermal processing technology of PVA established in our research group and biaxial stretching technology. The introduction of SP disrupted the original hydrogen bonds in modified PVA by forming new hydrogen bonds with the hydroxyl groups of each component in modified system, thus promoting the stable melt casting of PVA/SP composites and also endowing the obtained PVA/SP precursor sheets with good drawability. Upon biaxial stretching, SP reinforced the crystalline structure and orientation of PVA through their hydrogen bonds with PVA, improving the mechanical strength, crystallinity and thermal stability of PVA/SP films. The film with 3.0 × 3.0 stretching ratios demonstrated the exceptional tensile strength (62.2 MPa), tear strength (119.7 kN/m), low heat shrinkage (5.2 %), and oxygen permeability coefficient (1.38 × 10−16 cm3·cm/cm2·s·Pa), which surpassed most conventional plastic films used in food packaging field. This research not only pioneered an environmentally friendly packaging solution, but also offered a novel strategy for solid-state high-value, large-scale and economical utilization of waste crop straw, greatly avoiding the adverse effects of its burning on the environment.

Combined pretreatment of malic acid and kraft pulping for the production of fermentable sugars and highly active lignin

The separation and utilization of cellulose, hemicellulose, and lignin in lignocellulosic biorefineries present significant challenges. This study proposes a pretreatment method for biomass refining by combining acid and kraft pulping. Firstly, the biomass was pretreated by malic acid, resulting in the isolation of xylo-oligosaccharides (XOS) with a yield of 86.26 % with optimized conditions of 180 °C, 1 wt% concentration, 40 min. Secondly, a mixture of 12.98 wt% NaOH and 1.043 wt% Na2S is employed to achieve lignin removal efficiency up to 63.42 %. Physical refinement techniques are then applied to enhance the enzyme digestion efficiency of cellulose, resulting in an increase from 55.03 % to 91.4 % for efficient cellulose conversion. The reacted samples exhibit a lignin composition rich in β-O-4 ether bonds, facilitating their high-value utilization. The results indicated that the combined pretreatment approach demonstrates high efficiency in separating cellulose, hemicellulose, and lignin while obtaining XOS, highly active lignin, and enzyme-digested substrates.

ABA and MeJA Induced Catechin and Epicatechin Biosynthesis and Accumulation in Camellia oleifera Fruit Shells.

Camellia oleifera Abel, one of the most valuable woody oil plants, has been widely cultivated for extracting edible oil. The shell of C. oleifera is a by-product generated in the processing of edible oil extraction. However, there is still limited research on the maturity and high-value resource utilization of shell by-products. We found that the C. oleifera 'Huashuo' (HS) fruit shells contained a high content of catechins. Abscisic acid (ABA) and methyl jasmonate (MeJA) enhanced the accumulation of catechins in C. oleifera fruit shells, providing a basis for production and application of the catechins in fruit shells of C. oleifera. We further found that 500 μM ABA and 900 μM MeJA significantly promoted the accumulation of catechin (C) and epicatechin (EC) in fruit shells. Following treatment with 900 μM MeJA, the expressions of CoPAL1, CoC4H1, CoC4H2, CoC4H3, Co4CL1, Co4CL2, CoF3'H1, CoLAR1, CoLAR2, CoLAR3, CoANR2, and CoANRL2 were significantly upregulated, while after 500 μM ABA treatment the expressions of CoPAL3, CoCHS1, CoCHS4, CoF3'H1, CoDFR, CoLAR1, CoLAR2, CoLAR3, CoANS1, CoANR1, and CoANR2 increased dramatically. These results indicate that appropriate concentrations of ABA and MeJA activate C and EC biosynthesis and promote their accumulation in fruit shells. Our results provide new ideas and guidance for promoting the resource utilization of C. oleifera fruit shells.

Repair and Reuse of Spent Lithium Battery Electrode Materials

With the popularity of new energy vehicles and various electronic devices, the use of lithium-ion batteries (LIBs) has shown explosive growth, which has resulted in a large number of spent LIBs. Spent LIBs contain a large amount of metal resources, and improper disposal will not only cause waste of resources, but also have potential environmental risks. The existing commercial recycling methods are mainly pyrometallurgy and hydrometallurgy recycling methods, both of which require re-extraction from the electrode material after destroying them to the atomic level for the preparation of new electrode materials, which is a long process with high cost, and involves the application of extreme conditions such as high temperature and strong acid, with inferior economic and environmental benefits. It is a major challenge in the field of battery recycling to develop innovative and clean recycling methods, to simplify the recycling process and to develop ways to reuse the recovered products. In view of the challenge of existing recycling methods, the reporters proposed the idea of direct recycling of electrode materials at the molecular scale, and designed innovative recycling methods such as direct repair of degraded lithium cobalt oxides with deep eutectic solvent (DES), repair of Ni-Mn-Co ternary (NCM) cathode with high failure degree by low temperature molten salt, and thermal regeneration of degraded lithium iron phosphate (LFP) with multifunctional solvent; and closed-loop design of the direct recycling process. In addition, we propose a high-value utilization path for the conversion of degraded electrode materials to high-performance electrode materials and nano-catalysts. It greatly simplifies the recycling process, avoids the use of extreme conditions, and provides a new technical system and theoretical guidance for battery recycling research and industry.

Injection molded lightweight composites from tea-stem fiber and polypropylene: Effect of fiber loading on forming properties and cell structure

China boasts abundant tea resources and substantial production output. To facilitate the high-value utilization of by-products generated during tea production, such as tea stems, thereby reducing resource wastage, this study pioneered the preparation of tea-stem fiber-reinforced polypropylene (TF/PP) composites through microcellular injection and conventional molding processes. The effects of tea-stem fiber (TF) content on the formability of foamed and unfoamed composites were compared and analyzed. The findings reveal that the inclusion of TFs fostered the crystallization of polymer chains, decreased the flowability of the composites in their molten state, and enhanced the density, surface hydrophilicity, and thermal stability of the molded composites. In the unfoamed state, composites with 20 wt%, 40 wt%, and 30 wt% TF content exhibited optimal tensile (35.92 MPa), flexural (58.43 MPa), and impact (6.49 KJ/m2) strength, respectively. Upon foaming, composites with TF contents of 30 wt%, 40 wt%, and 30 wt% demonstrated superior tensile (32.84 MPa), flexural (51.31 MPa), and impact (6.94 KJ/m2) strength, respectively. A 30 % TF content in foamed composites yielded the best reinforcement effect, creating an ideal balance of internal interactions. This resulted in mechanical properties that were close to or even exceeded those of unfoamed PP while reducing the density by nearly 6 %. The results from this study contribute towards the high-value application of tea by-products, promoting the development of the global tea economy.

Structural changes and grading mechanism of lignin during solid alkali-active oxygen extraction and grading

Cooking with active oxygen and solid alkali (CAOSA) is an efficient pretreatment method for biomass. For better grading of the lignin yellow liquor, the different lignin fractions and the recovered solid alkali were obtained using a simultaneous acid-alkali graded separation method. The results indicated that the recovery rate of solid alkali was 67.25 %, and the grading of lignin components was characterized by smaller dispersion coefficients, and more stable properties and structure. Lignin fractions with low dispersion coefficients possess more key structures, including the Phenol hydroxyl group (ArOH), Methoxy (OMe), and β-aryl ether (β-O-4), and have better thermal properties. The low molecular weight L4 has the highest ArOH content (2.1 mmol/g), which provides better antioxidant properties. The CAOSA process destroyed the S-unit and prevented lignin from condensation. Furthermore, the CAOSA process protected carbohydrates, which could effectively prevent them from dehydrating and re-polymerizing into pseudo-lignin. This allowed the pulp to remain natural, which was beneficial for subsequent transformation and utilization. Overall, the efficient separation of biomass components and lignin grading method proposed by combining the CAOSA process with the acid-alkali grading separation method has a strong application prospect and provides a theoretical basis for the high-value utilization of biomass and lignin.

Research on interfacial fusion characteristics of rejuvenator-aged asphalt system: Wettability, permeability and molecular simulation

The research aims to illuminate the interfacial fusion mechanism of rejuvenator-aged asphalt to guide the development of high-performance rejuvenators and foster the high-value utilization of waste asphalt pavement materials. In the research, the contact angle between rejuvenator and aged asphalt was measured to assess its interface wettability, and the permeability and contributing factors in aged asphalt were profiled based on the permeation test and permeation degree parameter. Concurrently, the molecular dynamics simulations of the fusion behavior between rejuvenator and aged asphalt were conducted to comprehend the fusion mechanism deeply. Results reveal that thermal oxygen and photo-oxygen aging enhanced the asphalt mixture’s hydrophobicity with similar effects, and they also had comparable impacts on the wettability of rejuvenator-aged asphalt interface. A more considerable asphalt aging eventuated a more challenging for the rejuvenator to wet the asphalt surface naturally. Waste vegetable oil rejuvenators tended to have a superior wetting effect on the aged asphalt surface compared to mineral oil rejuvenators. Higher permeation temperatures and permeation durations facilitated the rejuvenator’s fusion with aged asphalt, and the permeability varied across different rejuvenators, hinging on the rejuvenator’s permeation performance and composition. The rejuvenator’s permeation process into light aged asphalt or aged matrix asphalt was prone to occur. The aging effect of asphalt negatively impacted the fusion process of the layered system. Compared to oleic acid, aromatic hydrocarbons exhibited superior permeation ability and better fusion effect with aged asphalt. Rejuvenator composition materials yielding high permeation and fusion effects should display the following characteristics: lower viscosity, weak molecular polarity, small molecular weight, linear molecular structure, or end phenyl ring structure.

Effects of steel slag mixed substrate on rooting of Hydrangea macrophylla cuttings.

To investigate the effect of steel slag used as substrate on the rooting of Hydrangea macrophylla cuttings, and to develop a new mixed substrate that can partially replace conventional cutting substrates and realize the high-efficient utilization of solid waste, we examined the physical and chemical properties of different mixed substrates containing 10% (T1), 20% (T2), 30% (T3), and 40% (T4) volume fractions of steel slag, and investigated the rooting of H. macrophylla 'Red Beauty' cuttings growing on these substrates, with conventional cutting substrates (peat and perlite) as the control (CK). The results showed that pH value, electrical conductivity, and bulk density of the mixed substrates were significantly higher than those of CK. The aeration porosity of T2 was higher than other treatments, while the total porosity and water holding porosity differed little from others. Both fresh weight and dry weight of all the four treatments were higher than those of CK, with stem diameter being higher than that of CK (except T4), plant height showing no significant difference compared to CK (except T4), and leaf chlorophyll content being significantly lower than CK. Root length ranked as T2>CK>T1>T3>T4, the root surface area and root volume both ranked as T2>T1>CK>T4>T3, the root tip ranked as T2>CK>T1>T4>T3. Both average root diameter and root activity were significantly higher than that of CK, with the highest value being observed in T2. Soluble sugar content in the leaves of T2 was the highest, followed by T4, T3, CK, and T1. The weight ranking of root growth indices was root activity > average root diameter > root volume > root surface area > root tip number > root length. Redundancy analysis indicated that pH value, electrical conductivity, aeration porosity, and water holding porosity of substrates were key factors influencing root growth and development of cuttings. Our results suggested that substrates mixed with 10% to 40% steel slag could be used for H. macrophylla cutting propagation, and 20% (T2) being the best one because it could significantly improve the survival rate, growth status, and root development of cuttings. Steel slag would be a novel substrate to partially replace conventional unrenewable substrates such as peat and perlite for flower seedling propagation, which could reduce agricultural production cost and provide a high-value utilization way of industry solid waste.

Improved combustion stability of biogas at different CO2 concentrations using inhomogeneous partially premixed stratified flames

Biogas is a renewable and clean energy source that plays an important role in the current environment of low- carbon transition. If high-content CO2 in biogas can be separated, transformed, and utilized, it not only realizes high-value utilization of biogas but also promotes carbon reduction in the biogas field. To improve the combustion stability of biogas, an inhomogeneous, partially premixed stratified (IPPS) combustion model was adopted in this study. The thermal flame structure and stability were investigated for a wide range of mixture inhomogeneities, turbulence levels, CO2 concentrations, air-to-fuel velocity ratios, and combustion energies in a concentric flow slot burner (CFSB). A fine-wire thermocouple is used to resolve the thermal flame structure. The flame size was reduced by increasing the CO2 concentration and the flames became lighter blue. The flame temperature also decreased with increase in CO2 concentration. Flame stability was reduced by increasing the CO2 concentration. However, at a certain level of mixture inhomogeneity, the concentration of CO2 in the IPPS mode did not affect the stability. Accordingly, the IPPS mode of combustion should be suitable for the combustion and stabilization of biogas. This should support the design of highly stabilized biogas turbulent flames independent of CO2 concentration. The data show that the lower stability conditions are partially due to the change in fuel combustion energy, which is characterized by the Wobbe index (WI). In addition, at a certain level of mixture inhomogeneity, the effect of the WI on flame stability becomes dominant.

Screening of a readily selenium-enriched Spirulina polysaccharide and characterization of its structure and bioactivity

The potential of selenium-containing polysaccharides as organic selenium nutritional supplements has attracted considerable interest; however, they also encounter obstacles, including the presence of impurities and a low selenium binding efficiency. In this study, a novel method combining Q-Sepharose Fast-Flow (QFF) column chromatography with the HNO3–Na2SeO3 method was proposed. Through the application of this methodology, the selenium binding efficiency of polysaccharides was enhanced by a factor of 3.41. The selenium binding capacities of rhamnose and glucose were the highest among the monosaccharides, reaching 770.7 mg/L and 955.3 mg/L, respectively. Spirulina polysaccharide-3 (SPS-3) was selected for further investigation because of its elevated total rhamnose and glucose contents and optimal selenium binding efficiency. Employing common assays, a comparative analysis of selenium-containing Spirulina polysaccharide-3 (SeCSPS) and SPS-3 is presented. Upon selenium binding, the surface morphology of the monosaccharides changed, whereas the main chain structure remained unaltered. The antihyperglycemic activity of SeCSPS was enhanced by selenium binding. Furthermore, both SeCSPS and SPS-3 can be degraded by the simulated digestive system. In conclusion, SeCSPS can be employed as an organic selenium supplement in food or nutraceuticals while also resulting in a high selenium content. The findings of this study will provide a viable avenue for the high-value utilization of Spirulina, which will help resolve the selenium deficiency issue faced by populations with low selenium intake globally.

Development of New Multi-Glycosylation Routes to Facilitate the Biosynthesis of Sweetener Mogrosides from Bitter Immature Siraitia Grosvenorii Using Engineered Escherichia coli.

Mogrosides, which have various pharmacological activities, are mainly extracted from Siraitia grosvenorii (Luo Han Guo) and are widely used as natural zero-calorie sweeteners. Unfortunately, the difficult cultivation and long maturation time of Luo Han Guo have contributed to a shortage of mogrosides. To overcome this obstacle, we developed a highly efficient biosynthetic method using engineered Escherichia coli to synthesize sweet mogrosides from bitter mogrosides. Three UDP-glycosyltransferase (UGT) genes with primary/branched glycosylation catalytic activity at the C3/C24 sites of mogrosides were screened and tested. Mutant M3, which could catalyze the glycosylation of nine types of mogrosides, was obtained through enhanced catalytic activity. This improvement in β-(1,6)-glycosidic bond formation was achieved through single nucleotide polymorphisms and direct evolution, guided by 3D structural analysis. A new multienzyme system combining three UGTs and UDP-glucose (UDPG) regeneration was developed to avoid the use of expensive UDPG. Finally, the content of sweet mogrosides in the immature Luo Han Guo extract increased significantly from 57% to 95%. This study not only established a new multienzyme system for the highly efficient production of sweet mogrosides from immature Luo Han Guo but also provided a guideline for the high-value utilization of rich bitter mogrosides from agricultural waste and residues.