Higher Hydrolysis Rate Research Articles

Influence of monomer composition of poly(butylene succinate-co-hexamethylene succinate)s on their enzymatic hydrolysis by Fusarium solani cutinase

Poly(butylene succinate) (PBS), poly(hexamethylene succinate) (PHS) and three kinds of poly(butylene succinate-co-hexamethylene succinate)s (P(BS-co-HS)s) were enzymatically hydrolyzed by Fusarium solani cutinase. The results showed that the characteristics of the enzymatic hydrolysis of these polyesters were mainly affected by crystallinity, thermal properties and the BS/HS ratio. The enzymatic hydrolysis rates of the polyesters are as follows: P(BS-co-HS52) ≈ P(BS-co-HS71) > PHS > P(BS-co-HS32) > PBS. Furthermore, with increasing HS content, both the degree of crystallinity (Xc) and melting temperature (Tm) of the polyesters first decreased and then increased. P(BS-co-HS52) and P(BS-co-HS71) had the lowest Xc and the lowest Tm, thus had the highest hydrolysis rate; this shows that the hydrolysis rate is affected by Xc and Tm. The results also showed that BS/HS ratio could affect the physical properties and degradability of polyesters. Thus, it is possible to prepare polyesters with various physical properties and degradability for different applications by adjusting BS/HS ratio. The crystalline and amorphous regions of the polyesters were both hydrolyzed, during which parts of the crystalline regions were converted into the amorphous regions. Finally, we found that the crystal structure and thermal stability of the polyesters were not affected by the enzymatic hydrolysis.

Characterization of chitinase from Exiguobacterium antarcticum and its bioconversion of crayfish shell into chitin oligosaccharides

Crayfish have become a heavily consumed food and its chitin-rich shell is of great value in terms of waste conversion. This study found a novel chitinase (EaChi40) from a marine bacterium Exiguobacterium antarcticum. The gene was cloned and expressed as a soluble protein of 40 kDa, having optimal activity at pH 6.0 and 30 °C. EaChi40 showed good stability and high specific activity, and kinetic studies found Km and Vmax were 0.86 mg/mL and 13.66 μmol/min/mg. For conversion crayfish shell into oligosaccharides, ball milling and ultrasound-assisted hydrogen peroxide decolorization were applied to pretreat crayfish shell to facilitate its hydrolysis. After the enzymatic conversion, the hydrolysis products of chitobiose and N-acetyl-D-glucosamine were 9.09 mg/mL and 9.21 mg/mL, respectively. EaChi40 efficiently degraded crayfish with a high hydrolysis rate of 76.1%. It is expected to be a good candidate for the production of chitin oligosaccharides in the food and biological fields.

Neglected effect of transportation on the property of municipal biowaste and the subsequent biomethane potential

Long-distance transportation of municipal biowaste can influence its physio-chemical characteristics and the subsequent anaerobic digestion (AD) performance. However, the effect of whole transportation processes on the characteristics of municipal biowaste from upstream source separation/collection to downstream storage remains poorly documented. To this end, this study presented the relevant work by sampling in practical conditions. Results showed that transportation led to a high hydrolysis rate of 33.4%–35.5%, and an acidification rate of 15.8%–17.3%. Simultaneously, lactic acid was produced to a concentration of 18.1 g/L, occupying approximately 20% of dissolved organic carbon. Furthermore, the highest cumulative CH4 production was observed at the samples of source separation/collection (398 ± 52–519 ± 117 mL/gVS for FW, 276 ± 51–332 ± 41 mL/gVS for KW), and then accompanied by a decrease (299 ± 31 mL/gVS for FW and 285 ± 39 mL/gVS for KW) after downstream storage. The results indicated that the subsequent anaerobic digestion would be inclined to appear perturbation caused by the lactic acidification of the initial feed and it is imperative to avoid further conversion of lactic acid to propionic acid, which could provide vital guideline reference for the optimization of biogas production process. Moreover, the lactic acid-rich property also highlights other value-added pathways for the resource recovery of municipal biowaste.

The hydrolysis dynamic of storage reserves in maize seed germination helps to explain differences in inbred lines and hybrid seed vigor

Abstract: Storage reserves composition is directly related to the manifestation of seed vigor. However, the physiological potential expression in inbred lines, as well as hybrids from the storage reserves hydrolysis, is not clear. Thus, the aim of this study was focused on verifying if there are differences in the hydrolysis of seed storage reserve of maize inbred lines or hybrids during germination, and also checking if the changes in hydrolysis are associated with seed vigor. The seeds of inbred lines and hybrids were submitted to germination test, vigor by accelerated aging and cold tolerance. Biochemical profiling (total protein content, soluble proteins, phytate, inorganic phosphorus, starch, and soluble sugars), was temporally determined at 0 h (quiescent seeds), 12 h, 24 h, 30 h, and 48 h after imbibition. The seeds of inbred lines showed a longer time for root protrusion and less vigor when compared to hybrids. The highest hydrolysis rates in inbred lines were observed in phase I and at the end of phase II of germination. In hybrids, the rates remained, on average, constant throughout germination. The greatest differences in the hydrolysis of reserve compounds occur in phase II of germination and differentiate the germination metabolism of hybrids and inbred lines.

Отбелка микрокристаллической целлюлозы, полученной методом газофазного гидролиза

One of the main consumers of microcrystalline cellulose (MCC) is the pharmaceutical industry, where MCC is used as a binder and filler in direct compression of tablets. MCC is produced by acidic hydrolysis of cellulose, which usually results in a decrease in whiteness. This is due to the destruction of sugars formed during hydrolysis and the subsequent formation of colored products. The composition and properties of these products depend on the method of hydrolysis, acid concentration, temperature, and process duration. One of the most promising methods for producing MCC is gas-phase hydrolysis of cellulose with hydrogen chloride gas-air mixtures. The method has a high rate of hydrolysis, low reagent and energy consumption. The requirements of the pharmaceutical industry determine the need to produce MCC with high whiteness. The research purpose is to select bleaching modes for MCC using sodium hypochlorite and hydrogen peroxide as bleaching agents. MCC produced by gas-phase hydrolysis of bleached wood pulp was used during the study. The whiteness and intensity of the yellow tint of MCC in the bleaching process were determined by digital colorimetry on a flatbed scanner. The paper shows that sodium hypochlorite and hydrogen peroxide allow achieving the whiteness not less than 90 % and the intensity of the yellow tint not more than 3 standard units. High-quality bleaching can be carried out even for MCC samples with an initial whiteness of about 40 %. The most effective bleaching agent is sodium hypochlorite when the pH of the bleaching solution is 2–3. Hydrogen peroxide also provides high whiteness of MCC at pH of 10–11. However, the consumption of active oxygen (AO) for bleaching is more than three times higher in comparison with the consumption of active chlorine (ACh). It was found that the dyes of MCC produced by gas-phase hydrolysis consist of two chromophore groups that decolorize at different rates. The easily oxidized group of components makes up about 90 % of the total amount of dyes, and the resistant to oxidation components make up about 10 % and determine the intensity of the yellow tint of MCC. The modes of bleaching MCC with sodium hypochlorite and hydrogen peroxide to product samples with whiteness comparable to that of imported samples were determined. For citation: Sizov A.I., Pimenov S.D., Stroiteleva A.D., Stroiteleva K.D. Bleaching of Microcrystalline Cellulose Produced by Gas-Phase Hydrolysis. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 6, pp. 173–183. DOI: 10.37482/0536-1036-2021-6-173-183

Screening of lactases suitable for the preparation of low-lactose prebiotic liquid milk and optimisation of their combination

Abstract Lactose intolerance is an important factor restricting the consumption of dairy products. Lactase is used to hydrolyze lactose in milk while generating galactooligosaccharides (GOS), thereby reducing the incidence of lactose intolerance. We used cow milk as raw materials, and selected enzyme preparations with high lactose hydrolysis rate and strong GOS generation ability from 14 commercially available lactase enzymes. The lactose hydrolysis rate is 5.85%-81.38%, and the GOS content is 0.03 g/L- 13.10 g/L. The mixing experiment design determined the two lactase enzymes (E10 and E11) ratio and the optimal enzymatic hydrolysis process of low-lactose prebiotic milk: compound lactases (E10:E11=0.756:0.244) addition 0.11%, 55℃for 5h, lactose hydrolysis rate and GOS content were 98.02% and 19.69g/L, respectively, and the remaining lactose content was about 0.97 g/L.

Sucrose Hydrolysis in a Continuous Packed-Bed Reactor with Auto-immobilise Aspergillus niger Biocatalyst Obtained by Solid-State Fermentation.

Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF). Fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions with various temperatures, sucrose concentrations, and feed flow rates. The SSF allowed obtaining a biocatalyst with an invertase activity of 82.2 U/g db. The biocatalyst maintained its activity in the range of 40 to 70°C for at least 70h of continuous operation. In a 20-mL packed bed reactor, the highest hydrolysis rate (12.3g/g db h) was obtained at 40°C with 2M sucrose. Continuous hydrolysis in 20-mL and 200-mL reactors at 60°C led to sucrose hydrolysis above 60% (8.5 residence times) and above 55% (4.5 residence times), respectively. The auto-immobilised biocatalyst produced by SSF without recovery, purification, and immobilisation stages offers an economical alternative for developing accessible biocatalysts that can be applied in batch or continuous sucrose hydrolysis processes. This study shows the potential of biocatalyst production by SSF for other enzymatic systems.

Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo

Ex vivo expansion of hematopoietic stem cells (HSCs) is one of the most promising strategies to increase the availability of transplantable HSCs and improve bone marrow transplantation outcomes. We recently demonstrated that mouse HSCs could be efficiently expanded in polyvinyl alcohol (PVA)-containing culture medium using only recombinant stem cell factor and thrombopoietin cytokines. However, the behavior of human HSCs in these simple PVA-based media was not fully elucidated. In this study, we analyzed the compatibility of PVA of different hydrolysis rates (HR) and molecular weights (MW) to support functional human and mouse HSCs ex vivo. Human and mouse HSCs proliferated more frequently in media containing PVA with lower HR than with higher HR, but both PVA types supported HSC multilineage reconstitution potential. Importantly, human HSCs cultured in PVA-containing media engrafted not only in irradiated recipients but also in non-irradiated recipients. Our results demonstrate that human HSCs can be maintained ex vivo using PVA-based culture systems and suggest approaches for future optimization of human HSC expansion.

Polymerization kinetics of tubulin from mung seedlings modeled as a competition between nucleation and GTP-hydrolysis rates.

Microtubules (MTs) form physiologically important cytoskeletal structures that are assembled by tubulin polymerization in nucleation- and guanosine triphosphate (GTP)-dependent manner. GTP hydrolysis competes with the addition of monomers, to determine the GTP-cap size, and the onset of shrinkage, which alternates with growth. Multiple theoretical models of MT polymerization dynamics have been reconciled to the kinetics of animal brain tubulins, but more recently, rapid kinetics seen in Arabidopsis tubulin polymerization suggest the need to sample a wider diversity in tubulin polymerization kinetics and reconcile it to theory. Here, we isolated tubulin from seedlings of Vigna sp. (mung bean), compared polymerization kinetics to animal brain tubulin, and used a computational model to understand the differences. We find that activity-isolated mung tubulin polymerizes in a nucleation-dependent manner, based on turbidimetry, qualitatively similar to brain tubulin, but with a 10-fold smaller critical concentration. GTP-dependent polymerization kinetics also appear to be transient, indicative of high rates of GTP hydrolysis. Computational modeling of tubulin nucleation and vectorial GTP hydrolysis to examine the effects of high nucleation and GTP-hydrolysis rates predicts a dominance of the latter in determining MT lengths and numbers. Microscopy of mung tubulin filaments stabilized by GMPCPP or taxol results in few and short MTs, compared to the many long MTs arising from goat tubulin, qualitatively matching the model predictions. We find GTP-hydrolysis outcompetes nucleation rates in determining MT lengths and numbers.

Multiple Sclerosis: Enzymatic Cross Site-Specific Hydrolysis of H1 Histone by IgGs against H1, H2A, H2B, H3, H4 Histones, and Myelin Basic Protein.

Histones play a key role in chromatin remodeling and gene transcription. Further, free histones in the blood act as damage-associated molecules. Administration of histones to animals results in systemic inflammatory and toxic effects. Myelin basic protein is the principal constituent element of the myelin-proteolipid sheath of axons. Abzymes (antibodies with catalytic activities) are the original features of some autoimmune diseases. In this study, electrophoretically homogeneous IgGs against H1, H2A, H2B, H3, and H4 histones and myelin basic protein (MBP) were isolated from the blood sera of multiple sclerosis (MS) patients by several affinity chromatographies. Using MALDI mass spectrometry, the sites of H1 histone cleavage by IgGs against H1, H2A, H2B, H3, H4, and MBP were determined. It was shown that IgGs against H1 split H1 at 12 sites, while the number of cleavage sites by abzymes against other histones was lower: H2A (9), H2B (7), H3 (3), and H4 (3). The minimum rate of H1 hydrolysis was observed for antibodies against H3 and H4. A high rate of hydrolysis and the maximum number of H1 hydrolysis sites (17) were found for antibodies against MBP. Only a few sites of H1 hydrolysis by anti-H1 antibodies coincided with those for IgGs against H2A, H2B, H3, H4, and MBP. Thus, the polyreactivity of complexation and the enzymatic cross-activity of antibodies against H1, four other histones, and MBP have first been shown. Since histones act as damage molecules, abzymes against histones and MBP can play a negative role in the pathogenesis of MS and probably other different diseases as well.

Assessment of electrooxidation as pre- and post-treatments for improving anaerobic digestion and stabilisation of waste activated sludge

This study evaluates the effects of electro-oxidation as a means for enhancing sludge stabilisation. Boron-doped diamond electrodes were used to treat waste activated sludge and digestate under different operating parameters (current density, conductivity, pH, and time). Electro-oxidation runs affected the solubilisation of organic matter, which seemed to improve anaerobic digestion and dewaterability characteristics.Among the tested parameters, pre-treating sludge via electro-oxidation under alkaline conditions (Treatment T5) resulted in the highest increase in soluble organic material compared to that in the control, with total organic carbon (TOC) and soluble chemical oxygen demand (COD) values of 2753 and 7819 mg L−1, respectively (control TOC and COD values were 385 and 1073 mg L−1). This pretreatment also achieved a high hydrolysis rate (higher concentration in volatile fatty acids) with a concomitant increase in methane yield (approximately 18%). On the other hand, the application of electro-oxidation as a post-treatment for improving digestate dewaterability resulted in noticeable changes in the release of water during drying due to protein and aliphatic matrix modification of the sample.

High-rate mesophilic co-digestion with food waste and waste activated sludge through a low-magnitude increasing loading regime: Performance and microorganism characteristics

To achieve a high-rate operation of co-digestion with waste activated sludge (WAS) and food waste (FW) for biogas production, a low-magnitude loading regime was investigated in a mesophilic continuous stirred tank reactor (CSTR) over long-term operation for approximately 600 days. The results showed that high-rate mesophilic CSTR co-digestion was realized using the low-magnitude loading regime. A maximum methane production of 12.9 L/L/day was achieved in the mesophilic CSTR co-digestion at an organic loading rate (OLR) of 48.1 g-COD/L/day. Moreover, high-efficiency and stable mesophilic CSTR co-digestion can still be performed at OLR of 50.8–52.1 g-COD/L/day and solid retention time (SRT) of 2.56–2.63 days without volatile fatty acid (VFA) accumulation. A high methane yield, hydrolysis conversion ratio, and methanogenic activity and the key anaerobic digestion enzymes were all maintained during the high-rate operation period. 16S rRNA gene sequencing results indicated that the relative abundance of the class Clostridia and genus Methanosarcina could reach 85.0% and 97.3%, respectively, corresponding to a high hydrolysis rate and VFA conversion rate. The metabolic capability of the genus Methanosarcina was the main reason for the highly efficient and stable operation of the mesophilic CSTR co-digestion. Using metagenomic analysis, Methanosarcina barkeri and Methanosarcina flavescens were established as the main methane-producing species during high-rate mesophilic CSTR co-digestion. The enrichment of the genus Methanosarcina through a low-magnitude loading regime is a promising method for realizing the highly efficient and stable operation of co-digestion with WAS and FW for biogas production at low retention times.

Artichoke pectic oligosaccharide characterisation and virtual screening of prebiotic properties using in silico colonic fermentation

The aim of this work was to develop a comprehensive workflow to elucidate molecular features of artichoke pectic oligosaccharides (POS) contributing to high potential prebiotic activity. First, obtainment of artichoke POS by Pectinex® Ultra-Olio was optimised using an artificial neural network. Under optimal conditions (pH 6.86; 1.5 h; enzyme dose 520.5 U/g pectin) POS yield was 624 mg/g pectin. Oligosaccharide structures (Mw < 1.3 kDa) were characterised by MALDI-TOF-MS. Then, conformational analysis of glycosidic bonds was performed by replica exchange molecular dynamics simulations and interaction mechanisms between POS and several microbial glycosidases were proposed by molecular modelling. Chemical information was integrated in virtual simulations of colonic fermentation. Highest hydrolysis rate was obtained for GalA-Rha-GalA trisaccharide, while the presence of partial negative charges and high radius of gyration enhance short chain fatty acid formation in distal colon. Established structure-activity relationships could help the rational design of prebiotics and clinical trials.

Expression and characterization of TbCel12A, a thermophilic endoglucanase with potential in biomass hydrolysis

Abstract To increase economic benefit and reduce pollutants, agricultural biomass can be hydrolyzed to monosaccharides, which serve as a feedstock for biorefinery production of fuels and fine chemicals. In a search for new enzymes to apply to this process, Thermobispora bispora TbCel12A endoglucanase was identified by rice straw compost metagenome analysis and expressed in recombinant Escherichia coli. TbCel12A hydrolyzed both soluble and insoluble β-glucan substrates. Its highest hydrolysis rate was observed for cellopentaose compared to other cellooligosaccharides, barley mixed linkage β-glucan, and carboxymethyl cellulose. Low hydrolysis rates were observed for α-cellulose, and avicel. The primary product detected was cellobiose and a smaller amount of glucose. TbCel12A exhibited highest activity at 65 °C and pH 5.0 and retained 86% relative activity after incubation at 60 °C overnight. TbCel12A conserved >80% activity in 10% alcohol and at high salt concentrations. Hydrolysis of pretreated rice straw using commercial cellulase and TbCel12A released slightly more reducing sugars than hydrolysis using only commercial cellulase. Therefore, TbCel12A is a promising enzyme for further development for use in biomass conversion.

Purification and characterization of a cold-adapted κ-carrageenase from Pseudoalteromonas sp. ZDY3

κ-Carrageenase (EC3.2.1.83) is a class of glycoside hydrolase, which can be used for hydrolysis of κ-carrageenan to κ-carrageenan oligosaccharides. In this study, a bacterium, identified as Pseudoalteromonas sp. ZDY3 isolated from rotten algae, was capable to degrade κ-carrageenan. The κ-carrageenase produced by Pseudoalteromonas sp. ZDY3 was purified to homogeneity and named as CgkZDY3. The accurate molecular mass of CgkZDY3 was determined through LC-HRMS, and a posttranslational removal of C-terminal end of the protein was discovered. CgkZDY3 had strict hydrolysis specificity to κ-carrageenan, the values of Km and kcat/Km of CgkZDY3 were 3.67 mg mL−1 and 53.0 mL mg−1 s−1, respectively. CgkZDY3 was a cold-adapted κ-carrageenase with excellent storage stability of both the temperature below 35 °C and a wide pH range, and was an endo-type κ-carrageenase with high hydrolysis rate, oligosaccharides with different degrees of polymerization can be obtained by controlling the hydrolysis time, and the final products were κ-neocarrabiose and κ-neocarratetraose. These properties are of great significance for production of κ-carrageenan oligosaccharides with different polymerization degrees under process control.

Hydrolysis of eutectic compositions in the ZnCl2:KCl:NaCl ternary system and effect of adding ZnO

Molten salt systems have been considered as proper liquefiers, solvents and transfer media due to their transport and thermodynamic properties. Employing molten salts to liquify biomass could be performed to make it pumpable and transfer it more easily through thermochemical conversion processes to e.g. bio-oil. The first challenge for this application is to find a salt with relatively low melting point. It needs to be low enough to avoid producing ash or char and at the same time high enough to liquify biomass. The selected molten salt requires high thermal stability to avoid salt decomposition at high temperatures and make salt recycling possible. Another challenge is minimising the hydrolysis rate of the molten salt in contact with water molecules originated from the biomass, because this can lead to undesired formation of highly corrosive acids. ZnCl2:KCl:NaCl is a promising molten salt with relatively low melting point, high thermal stability and good properties in contact with biomass. The objective of this work is to investigate the properties of the eutectic mixtures of ZnCl2:KCl:NaCl that are of importance for thermochemical conversion of biomass. Four compositions of ZnCl2:KCl:NaCl are investigated, including Salt #1: 60: 20: 20, Salt #2: 59.5: 21.9: 18.6, Salt #3: 52.9: 33.7: 13.4 and Salt #4: 44.3: 41.9:13.8 in mole fraction. Salt #4 is found to exhibit the best properties, with low melting point, the highest thermal stability and the lowest hydrolysis rate. Salt #1 had the highest hydrolysis rate. However, addition of ZnO showed a marked, limiting effect on the hydrolysis, especially at temperatures below 400 °C.

Heuristic methods in optimization of selected parameters of Two-Phase Anaerobic Digestion (TPAD) model

The article aims to define and optimize the key parameters of Two-Phase Anaerobic Digestion model (TPAD). This kind of anaerobic digestion utilizes a separation of selected process steps in two, different reactors. The reason for this modification is to achieve the best possible conditions for all reactions and transformations – which are significantly different for hydrolysis/acidogenesis and acetogenesis/methanogenesis. It can be done differently: by pH control, selective inoculation and so one. However this article focus on temperature phasing, in which the first step is kept in thermophilic conditions, which promotes a high rate of hydrolysis, while the second is mesophilic, usually in higher pH, which expands optimal conditions for methane generating archaea. Thus the model parameters in each of two reactors have to be adjusted to the specific environmental conditions. In this work, as the most important, three reaction rates were recognized: for hydrolysis (kdis), for propionate-related conversions (km_pro), and acetate-related conversions (km_ac). The optimization leads to the conclusion that in the value of km_pro and km_ac decrease with the temperature, while the temperature-dependence coefficient is accordingly 0.019487°C-1 and 0.01068°C-1. The hydrolysis rate indicated the opposite trend, and the coefficient value was -0.0534°C-1. The reference reaction rates are defined for 35°C and are respectively: 7.964313, 12.3536, 1.048912 d-1. To calculate these values, a two-stage heuristic algorithm was utilized, starting with initial values based on selected other models available in the literature. The data to validation was taken from the most complete and most recent publications and scientific databases.

Nano iron materials enhance food waste fermentation

Anaerobic fermentation can produce valuable chemicals from food waste, such as butyric acid, lactic acid, and ethanol. Three nano iron materials, i.e. zero-valence iron (nZVI), ferric oxide (nFO), and magnetite (nM), were tested to improve food waste fermentation with mixed inoculation. nZVI and nFO enhanced the dissolution and conversion of carbohydrate to lactate, dependent on different mechanisms, while nM had almost no effects due to its aggregation at high doses. nZVI alleviated the drop of pH, kept the ORP at an anaerobic level, increased functional enzymes, and resulted in a high hydrolysis rate of 45% and lactic acid yield of 0.16 g/g VSfed at the dose of 500 mg/(Lfed·d). nFO increased the ORP to 100 mV, stimulated the enrichment of Pseudomonas, and increased the abundance of Lactobacillus_panis, leading to hydrolysis rate of 47% and lactic acid yield of 0.19 g/gVSfed at the dose of 200 mg/(Lfed·d).

Formulating protein-based beverages for the dysphagia diets of the elderly: viscosity, protein quality, in vitro digestion, and consumers acceptability.

Dysphagia is defined as a disorder of the swallowing mechanism. The most common management of dysphagia is diet modification by thickening food and beverages. This study aimed to obtain protein-based beverages for the dysphagia diets of the elderly, corresponding to the 'honey' (III) level of dysphagia fluids according to the National Dysphagia Diet classifications, and containing 100 g kg-1 of good-quality proteins with a high rate of hydrolysis during digestion. Four protein formulations made from pea proteins, milk proteins, a mixture of milk and pea proteins, and milk proteins with added konjac glucomannan, were evaluated on the basis of rheological characterization and proteolysis kinetics during in vitro digestion. The mixture of milk proteins and pea proteins, and the mixture of milk proteins with added konjac glucomannan, showed typical yielding pseudoplastic fluid behavior with similar apparent viscosity but different structural characteristics. These differences were the reason for the differences in proteolysis kinetics during digestion. The mixture of milk and pea proteins showed viscous liquid behavior and was more rapidly hydrolyzed under gastrointestinal conditions than mixtures containing milk proteins and konjac glucomannan acting as a weak gel system. We presume that geriatric consumers with swallowing difficulties may benefit from 'honey'-level viscosity, protein-based beverages containing pea and milk proteins through faster proteolysis and better bioaccessibility of amino acids during digestion. © 2020 Society of Chemical Industry.

Effect of ultrasound on goat cream hydrolysis by lipase: Evaluation on enzyme, substrate and assisted reaction

The effect of ultrasound on lipase performance was studied in goat cream hydrolysis. Ultrasound (25 kHz, 22 W/L) was carried out on lipase and/or goat cream under different conditions (pH 6.0; temperature of 25, 40 and 55 °C; up to 5 h). As a pre-treatment, ultrasound did not induce goat cream hydrolysis, but slightly increased lipase activity (up to 12%), depending on the temperature and processing time (activation conditions at 25 °C/45 min and 40 °C/60 min). The fat hydrolysis assisted by ultrasound had higher hydrolysis rate when compared to conventional processing with an increase of 12% at 55 °C, 23% at 40 °C and 28% at 25 °C. The reaction temperature was an important parameter and lower temperatures improved the ultrasound effects. Possibly, these results are associated with the acceleration of mass transfer during ultrasound reaction, as well as the modifications promoted by ultrasound on the enzyme. Therefore, ultrasound was demonstrated as an interesting alternative to improve the goat cream hydrolysis by lipase.