Trains are becoming a popular way of transportation driven by comfort and ecology reasons. Latest statistics showed an increasing of 40% on the number of passengers in the last decade. The development of new high-speed trains promoted an evolution on the coaches interiors, as to make railway transportation more attractive. To cope this objective, new requirements were set, namely high levels of comfort and safety. In complement, multiple long-term ride comfort evaluation methods have been developed. The aim of this work is to present a review on the passengers’ comfort perception in railway vehicles. The standards ISO 2631, EN 12299 and the Sperling’s method are the most used ones. They refer several factors, as the vibration (level, frequency and duration), temperature, noise and area of the train per capita. Additionally, the perception of reduced accidents delayed the studies of passive security. Therefore, recent works focus on reducing the consequences of the second impact in case of accident and minimize the biomechanics injury criterion, through new interiors design layouts.
 Keywords: Railway vehicles, Passengers comfort, Posture, Ride evaluation, Passive safety