Higher Leaf Biomass Research Articles

Fodder Biomass Monitoring in Sahelian Rangelands Using Phenological Metrics from FAPAR Time Series

Timely monitoring of plant biomass is critical for the management of forage resources in Sahelian rangelands. The estimation of annual biomass production in the Sahel is based on a simple relationship between satellite annual Normalized Difference Vegetation Index (NDVI) and in situ biomass data. This study proposes a new methodology using multi-linear models between phenological metrics from the SPOT-VEGETATION time series of Fraction of Absorbed Photosynthetically Active Radiation (FAPAR) and in situ biomass. A model with three variables—large seasonal integral (LINTG), length of growing season, and end of season decreasing rate—performed best (MAE = 605 kg·DM/ha; R2 = 0.68) across Sahelian ecosystems in Senegal (data for the period 1999–2013). A model with annual maximum (PEAK) and start date of season showed similar performances (MAE = 625 kg·DM/ha; R2 = 0.64), allowing a timely estimation of forage availability. The subdivision of the study area in ecoregions increased overall accuracy (MAE = 489.21 kg·DM/ha; R2 = 0.77), indicating that a relation between metrics and ecosystem properties exists. LINTG was the main explanatory variable for woody rangelands with high leaf biomass, whereas for areas dominated by herbaceous vegetation, it was the PEAK metric. The proposed approach outperformed the established biomass NDVI-based product (MAE = 818 kg·DM/ha and R2 = 0.51) and should improve the operational monitoring of forage resources in Sahelian rangelands.

Quantitative trait loci associated with constitutive traits control water use in pearl millet [Pennisetum glaucum (L.) R. Br

There is substantial genetic variation for drought adaption in pearl millet in terms of traits controlling plant water use. It is important to understand genomic regions responsible for these traits. Here, F7 recombinant inbred lines were used to identify quantitative trait loci (QTL) and allelic interactions for traits affecting plant water use, and their relevance is discussed for crop productivity in water-limited environments. Four QTL contributed to increased transpiration rate under high vapour pressure deficit (VPD) conditions, all with alleles from drought-sensitive parent ICMB 841. Of these four QTL, a major QTL (35.7%) was mapped on linkage group (LG) 6. The alleles for 863B at this QTL decreased transpiration rate and this QTL co-mapped to a previously detected LG 6 QTL, with alleles from 863B for grain weight and panicle harvest index across severe terminal drought stress environments. This provided additional support for a link between water saving from a lower transpiration rate under high VPD and drought tolerance. 863B alleles in this same genomic region also increased shoot weight, leaf area and total transpiration under well-watered conditions. One unexpected outcome was reduced transpiration under high VPD (15%) from the interaction of two alleles for high VPD transpiration (LG 6 (B), 40.7) and specific leaf mass and biomass (LG 7 (A), 35.3), (A, allele from ICMB 841, B, allele from 863B, marker position). The LG 6 QTL appears to combine alleles for growth potential, beneficial for non-stress conditions, and for saving water under high evaporative demand, beneficial under stressful conditions. Mapping QTL for water-use traits, and assessing their interactions offers considerable potential for improving pearl millet adaptation to specific stress conditions through physiology-informed marker-assisted selection.

Comparative Biomass Production of Six Oat Varieties in a Saline Soil Ecology

Oat is widely used as a food source in human and animal diets. In China, supplementary cultivation of oat has recently been extended into marginal saline soils, due to its extensive use. The goal of the present study was to explore and compare the agronomic characteristics, biomass production, and distribution of oat in coastal saline soil. A single-factor randomized block design experiment with six naked and husked oat varieties [Bayou-1 (BU-1), Baiyan-2 (BY-2), Baiyan-7 (BY-7), Huawan-6 (HW-6), Huazao-2 (HZ-2), and Pin-16 (P-16)] as treatments was conducted in the Hebei Province of China. The growth period (77 to 88 days) of the six oat varieties were shorter in saline soil than their original breeding region. Moreover, the plant was shorter, with a lower grain number per plant and seed setting. Grain weight increased and no change was observed in the floret number in coastal saline soil relative to the original habitats. The BY-7 variety produced the highest biomass value of 7.0 t ha−1 compared to the other five varieties. Compared with two active growth points, most varieties in the present study showed just one growth activity point between elongation and heading. Two fast growth periods for the six oat varieties were due to the high total leaf biomass growth rate (BGR) from elongation to heading and panicle BGR from heading to maturity. The biomass growth ratio (BGO) during elongation to heading was the largest among the four growth stages of the oat varieties. The number of kernels per spike decreased at the research site, but grain weight increased. Substantial differences were observed for growth periods, biomass, BGR, BGO, and other agronomic characters in the coastal saline soil of North China, but the BY-7 variety was the most suitable variety for the study site.

Effects of leaf zeatin and zeatin riboside induced by different clipping heights on the regrowth capacity of ryegrass

AbstractThe effect of clipping height on ryegrass regrowth was investigated by examining the roles of several plant hormones. Our study consisted of three treatment conditions: (1) darkness over whole plants, (2) darkness only over stubble leaf sheaths, and (3) light over whole plants. Results showed that under darkness over whole plant, low stubble height resulted in low leaf regrowth biomass. Similar leaf regrowth biomass was observed under conditions of darkness only over stubble leaf sheaths as well as light over whole plants. Each unit weight of stubble at different clipping heights has relatively similar potential of providing stored organic substance for leaf regrowth. Therefore, regrowth index, calculated as newly grown leaf biomass divided by unit stubble weight, was used to evaluate regrowth capacity at different clipping heights under minimal influence of organic substances stored in stubbles. Under light over whole plants and single clipping, low stubble height and high stubble height with root thinning resulted in low leaf biomass and high regrowth index. On the other hand, under light over whole plants and frequent clipping high leaf biomass and regrowth index were observed in high stubble height. In addition, we found that leaf zeatin and zeatin riboside (Z + ZR) affected ryegrass regrowth and that roots regulated leaf Z + ZR concentration. Thus, our results indicate that root‐derived cytokinin concentration in leaves influences ryegrass regrowth at different clipping heights.

Seasonal coupling of canopy structure and function in African tropical forests and its environmental controls

Tropical forests provide important ecosystem services in maintaining biodiversity, sequestering carbon and regulating climate regionally and globally. Climate triggers the seasonal transitions of vegetation structure and function in tropical forests. In turn, the seasonal cycles of structure and function in tropical forests feed back to the climate system through the control of land‐atmosphere exchange of carbon, water and energy fluxes. Large uncertainties exist in the carbon and water budgets of tropical forests, and environmental controls on phenology are among the least understood factors. Although field studies have identified patterns in the environmental controls on local‐scale species‐level phenology in the tropics, there is little consensus on large‐scale top‐down environmental controls on whole‐ecosystem seasonality. In this paper, we use both optical and microwave remote sensing to investigate the seasonality of vegetation canopy structure and function in three distinct tropical African forest types, and identify environmental triggers or controls of their variability. For most tropical forests that have a closed canopy and high leaf biomass, optical remote sensing (e.g., vegetation indices) captures canopy photosynthetic capacity (i.e., canopy function), while small‐wavelength microwave remote sensing characterizes the leaf biomass and leaf water content of the upper canopy (i.e., canopy structure). Our results reveal a strong coupling of canopy structure with canopy function in the tropical deciduous forests and woody savannas, and their seasonalities are both controlled by precipitation rather than solar radiation. By contrast, tropical evergreen forests in Africa exhibit a decoupling of canopy structure from canopy function revealed by different sensors: canopy photosynthetic capacity shown by the optical remote sensing is linked to the seasonal variation of precipitation, while microwave remote sensing captures semi‐annual leaf‐flushing that is synchronous with peak insolation intensity at the top of the atmosphere, which is bimodal. The differential coupling of canopy structure and function in tropical forests observed from remote sensing highlights differences inherent in distinct vegetation types within the tropics that may originate in the different life histories of their respective floras. This satellite‐based finding encourages more field‐based studies to clarify the interpretation of these large scale patterns.

English

  Low leaf biomass yields coupled with small land sizes are some of the major constraints faced by smallholder farmers that grow protein-rich fodder trees and shrubs. Given these challenges, availability of highly productive seed sources would be important to enable farmers to produce leaf fodder in sufficient quantities. 14 accessions of Acacia angustissima were evaluated for leaf, wood and total biomass production at two subtropical sites with uni-modal rainfall in Zimbabwe, with the objective of identifying high leaf biomass yielding accessions. There were up to fourfold difference in biomass yield between the accessions. Leaf dry matter yield ranged from 1.65 to 8.81 Mg ha-1 and 3.7 to 12.4 Mg ha-1 for wood biomass at the higher altitude site (1530 m a.s.l.) but were much lower at 1272 m a.s.l. where they ranged between 0.37 and 4.88 Mg ha-1 for leaf and 0.4 and 7.2 Mg ha-1 for wood. The most productive accessions for leaf biomass were 16231 and 18579 at the higher altitude site, while 18586 and 18501 had the highest yields at the lower altitude. Although no one accession was consistently high yielding across the two sites, the least productive accessions were consistently poor at both sites. The advantage of using selected superior accessions over the unselect bulk seed was up to 85%. These findings underscore the need to promote the use of only high yielding accessions rather than unselect bulk seed. This study identified new, more productive accessions of A. angustissima that potentially widens the genetic base of the germplasm assembled in Zimbabwe.   Key words: Leaf biomass, accession, accession × site interaction, Acacia angustissima.

Genotype–environment interaction in Gliricidia sepium: Phenotypic stability of provenances for leaf biomass yield

Limited multi-location testing has been undertaken in Gliricidia sepium and other agroforestry species compared to field crops and plantation forest tree species. Consequently, information on the stability of the various provenances (accessions) of this species is very limited, reducing the efficiency of germplasm deployment. In this study, genotype–environment interaction and phenotypic stability of 14 accessions were investigated for dry matter (DM) leaf biomass yield across five diverse sites in the tropics (Indonesia and Nigeria) and subtropics (Australia, Malawi and Zambia) using nonparametric methods. Results indicated that genotype–environment interaction is present and that some accessions were unstable across these diverse environments implying that blanket recommendation for germplasm deployment may not be efficient. The most unstable accessions for leaf biomass production in this study include the widely used Retalhuleu provenance, which although highly productive, appeared to be better suited to tropical wetter environments. The Belen Rivas and Monterrico provenances were found to combine high phenotypic stability and high DM leaf biomass yield across these diverse sites. Germplasm of these seed sources may be deployed in previously untested sites with perhaps, predictable DM leaf yields. Where further breeding and selection is envisaged, the results of this study could also be used to make selections and constitute breeding populations for either specific environments (Retalhuleu) or for diverse environments (Belen Rivas and Monterrico).

Adaptation and agronomic performance of Artemisia annua L. under lowland humid tropical conditions

Demand for new chemotherapies against malaria is increasing as Plasmodium, the causative organism of the disease, has shown a high degree of resistance against most standard anti-malarial drugs. One of the few highly effective compounds is artemisinin, the major sesquiterpene for the production of artemisinin-based combination therapies (ACTs). This antimalarial drug is extracted from the leaves of Artemisia annua, a plant that is mainly cultivated under cool (temperate) climate and high altitudes. The current study examined the possibility of introducing A. annua germplasm into the agricultural landscape of the lowland humid tropics in field experiments and identified promising accessions that are capable of producing high leaf biomass and artemisinin yields. Differences amongst seed origin, planting season and soil moisture availability had highly significant (P<0.01) effects on most of the agronomic characteristics evaluated, suggesting that these are very critical factors when considering the cultivation of A. annua in the humid tropics. Two non-photoperiodic hybrid lines evaluated in the trials had suitable agronomic potential, including the production of fresh leaf biomass yield in excess of 30 tonnes/ha with up to 1.0975% artemisinin concentration. This study shows that with improved management practices and using the right accessions, there is an inspiring possibility that the cultivation of A. annua can be optimized in the humid tropics for commercial production of the life saving artemisinin drug, which is most needed in these regions where malaria is endemic.

Dry matter allocation in Medicago arborea and Medicago citrina in response to drought and defoliation

Soil water availability is one of the main factors determining plant growth and forage production. The effects of soil water deficit on the development of two woody Mediterranean Medicago species, M. arborea and M. citrina were studied. A field experiment was carried out in the University of the Balearic Islands (Spain), under irrigated and drought conditions on both non-defoliated plants (NDP) and defoliated plants (DP). Under drought, all studied parameters for NDP were affected by water stress in summer, though there were no significant differences between species for shoot biomass (B). However, M. citrina maintained significantly higher leaf biomass than M. arborea, which represents a great part of B. On the contrary, M. arborea plants suffered total leaf senescence in summer, and B was totally composed of woody parts. For DP, spring–autumn was a favourable period for plant growth and development, under both water regimes. In well irrigated M. citrina plants, and compared with M. arborea, the capacity of regrowth was higher, and leaf area was similar for NDP and for DP. During summer, the regrowth was sensitive to the extreme temperatures. In drought conditions, the capacity of regrowth was relatively favoured in spring but completely inhibited in summer for both species.

Abundance, edge effect, and seasonality of fauna in mixed-species seagrass meadows in southwest Sulawesi, Indonesia

Motile fauna species in two mixed-species seagrass meadows with different canopy structure were studied on an uninhabited island in the Spermonde Archipelago, Sulawesi, Indonesia. The main focus of the study was to assess the edge effect and seasonal abundance of macrobenthic invertebrates. Fish and infauna densities were determined as well. Fauna was counted using permanent transects (macrobenthic invertebrates), visual census (fish species), and sediment cores (infauna). Both meadows had a comparable distribution of motile fauna species with polychaetes (35% of total abundance), bivalves (27%) and sipunculids (25%) accounting for the largest part of the total faunal abundance. The closed canopy meadow (high seagrass leaf biomass) had an overall higher faunal abundance compared with the open canopy meadow (low seagrass leaf biomass) (1133 vs. 751 individuals m−2). Although infauna abundance was comparable between the meadows, macrobenthic invertebrates (crustaceans, echinoderms, and molluscs) and fishes were more abundant in the closed canopy meadow, with only a few individual species more abundant in the open canopy meadow. The effect of distance from the meadow edge on macrobenthic invertebrate abundance was significant, with higher abundances towards the interior of the seagrass meadows, but for fish abundance no significant differences were found. Effects of seasonality (rainy vs. dry season) on macrobenthic invertebrate abundance were only significant for molluscs. We concluded that macrobenthic invertebrate abundance was most influenced by seagrass canopy structure, followed by meadow edge effects, and least by seasonality. Comparisons of faunal abundance in seagrass meadows need thus to include information on these three variables.

Farmers’ agronomic and social evaluation of productivity, yield and N2-fixation in different cowpea varieties and their subsequent residual N effects on a succeeding maize crop

Cowpea–maize rotations form an important component of the farming systems of smallholder farmers in the forest/savannah transitional agro-ecological zone of Ghana. We evaluated five cowpea varieties for grain yield, N2-fixation, biomass production, and contribution to productivity of subsequent maize grown in rotation. We further analyzed the interrelationship between these technical dimensions and the social acceptability of these cowpea varieties for farmers. Cowpea grain yield ranged between 1.1 and 1.4 t ha−1 with no significant yield differences among the different varieties. Using the 15N natural abundance technique, the average proportion of N2 fixed ranged between 61% for Ayiyi and 77% for Legon prolific. This resulted in average amounts of N2 fixed in above-ground biomass ranging between 32 and 67 kg N ha−1, respectively. Variation in estimates due to differences in δ15N among reference plants were larger than differences between cowpea varieties. The amount of soil-derived N ranged from 15 to 20 kg N ha−1. The above-ground net N contribution of the cowpea varieties to the soil (after adjusting for N export in grains) was highest for Legon Prolific (31 kg N ha−1) due to high N2-fixation and high leaf biomass production. Maize grain yield after cowpea without application of mineral N fertilizer ranged between 0.4 t ha−1 with maize after maize to 1.5 t ha−1 with Legon Prolific. The N fertilizer equivalence values for the cowpea varieties ranged between 18 and 60 kg N ha−1. IT810D-1010 was ranked by the farmers as the most preferred cowpea variety due to its white seed type, short-duration, ease of harvesting and good market value. Despite the high leaf biomass production and high amount of N2 fixed by Legon Prolific, it was generally the least preferred variety due to lower market price, late maturity, least potential cash income (due to the red mottled seed type) and difficulty in harvesting. Although farmers recognized the contribution of cowpea to soil fertility and yields of subsequent maize, they did not consider this as an important criterion for varietal selection. Soil fertility improvement must be considered as an additional benefit rather than a direct selection criterion when designing more sustainable smallholder farming systems.

Warm and cold parental reproductive environments affect seed properties, fitness, and cold responsiveness in Arabidopsis thaliana progenies

Conditions in the parental environment during reproduction can affect the performance of the progenies. The goals of this study were to investigate whether warm or cold temperatures in the parental environment during flowering and seed development affect Arabidopsis thaliana seed properties, growth performance, reproduction and stress tolerance of the progenies, and to find candidate genes for progeny-related differences in stress responsiveness. Parental plants were raised at 20 degrees C and maintained from bolting to seed maturity at warm (25 degrees C) or cold (15 degrees C) temperatures. Analysis of seed properties revealed significant increases in nitrogen in seeds from warm temperature and significant increases in lipids and in the ratio of alpha-linolenic to oleic acid in seeds from the cold parental environment. Progenies of the warm parental environment showed faster germination rates, faster root elongation growth, higher leaf biomass and increased seed production at various temperatures compared with those from the cold parental environment. This indicates that under stable environmental conditions, progenies from warm parental environments had a clear adaptive advantage over those from cold parental environments. This parental effect was presumably transmitted by the higher nitrogen content of the seeds developed in warm conditions. When offspring from parents grown at different temperatures were exposed to chilling or freezing stress, photosynthetic yield recovered faster in progenies originating from cold parental environments. Cold acclimation involved up-regulation of transcripts of flavanone 3-hydroxylase (F3H) and pseudo response regulator 9 (PRR9) and down-regulation of growth-associated transcription factors (TFs) NAP and AP2domain containing RAP2.3. NAP, a regulator of senescence, and PRR9, a temperature-sensitive modulator of the circadian clock, were probably involved in mediating parent-of-origin effects, because they showed progeny-related expression differences under chilling. Because low temperatures also delay senescence, cold responsiveness of NAP suggests that this factor is linked with the regulatory network that is important for environmental acclimation of plants.

MODIS land cover and LAI collection 4 product quality across nine sites in the western hemisphere

Global maps of land cover and leaf area index (LAI) derived from the Moderate Resolution Imaging Spectrometer (MODIS) reflectance data are an important resource in studies of global change, but errors in these must be characterized and well understood. Product validation requires careful scaling from ground and related measurements to a grain commensurate with MODIS products. We present an updated BigFoot project protocol for developing 25-m validation data layers over 49-km <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2 </sup> study areas. Results from comparisons of MODIS and BigFoot land cover and LAI products at nine contrasting sites are reported. In terms of proportional coverage, MODIS and BigFoot land cover were in close agreement at six sites. The largest differences were at low tree cover evergreen needleleaf sites and at an Arctic tundra site where the MODIS product overestimated woody cover proportions. At low leaf biomass sites there was reasonable agreement between MODIS and BigFoot LAI products, but there was not a particular MODIS LAI algorithm pathway that consistently compared most favorably. At high leaf biomass sites, MODIS LAI was generally overpredicted by a significant amount. For evergreen needleleaf sites, LAI seasonality was exaggerated by MODIS. Our results suggest incremental improvement from Collection 3 to Collection 4 MODIS products, with some remaining problems that need to be addressed

Evaluation of the temporal and spatial distribution of non-methane hydrocarbon emissions from the forests inSlovakia

The paper presents the first detailed inventory of non-methane hydrocarbon emissions from forest vegetation in Slovakia. Emissions rates of monoterpene, isoprene and other volatile organic compounds (VOC) were calculated for each main tree species separately using the relevant parameters such as temperature and leaf biomass density. The GIS technology was used for the analysis of spatial distribution of VOC emissions. The annual total VOC emissions from forests in Slovakiaranged from 77 to 98 ktonnes during the period 1990&amp;ndash;2000. The percentage of isoprene, mono-terpene, and other VOC emissions is relatively stable and varies in the narrow intervals: isoprene emissions (17&amp;ndash;23%), monoterpene emissions (65&amp;ndash;73%), and other VOC emissions (10&amp;ndash;15%). The major contribution of VOC (especially monoterpenes) comes from the Norway spruce (Picea abies) forests (45.7% in 2000) due to their abundance and high leaf biomass density with domination in the montane belt. The oak species (Quercus sp.) are the second important VOC emittors (18.2%) with domination in the thermo-colline and colline belt of the Carpathian Mts.

Effects of mining wastes on a seagrass ecosystem: metal accumulation and bioavailability, seagrass dynamics and associated community structure

Two different Cymodocea nodosa (Ucria) Ascherson beds growing in mining-contaminated sediments were compared with two reference beds in the Mar Menor coastal lagoon. The accumulation of Zn, Pb and Cd in different fractions of the plant, the sediment parameters that regulate the availability of metals, the seabed structure and dynamics of each seagrass bed and its associated macroinvertebrate community were studied. C. nodosa accumulates metals from the sediments and reflects their bioavailability for this seagrass. At each station, the metal content of the rhizomes was lower than that of leaves and roots. The concentration of acid-volatile sulfides does not seem to influence the availability of metals to the seagrass, possibly due to oxygen transport to underground tissues. The highest metal concentration in all the contaminated stations was found in the leaf-biofilm, due to the formation of complexes between metals and the extracellular polymeric substances that form the biofilm. All the seagrass beds were seen to be undergoing expansion, those growing in contaminated sediments accumulating great quantities of metals and showing highest photosynthetic leaf surface area and highest leaf biomass. However, these structural parameters were not seen to be responsible for the differences in the faunal composition observed between contaminated and reference beds. Moreover, the multivariate analysis identified the metal content of leaves, biofilm and sediments as important variables that may be responsible for these differences in faunal composition. In this study we have demonstrated that both the seagrass C. nodosa and the biofilm on the plant leaves may be used as environmental tools in the Mar Menor lagoon. The former is an useful indicator of sediment contamination, whereas the latter seems to be a good sentinel of water quality.

Análisis de crecimiento, área foliar especí­fica y concentración de nitrógeno en hojas de pasto "mulato" (Brachiaria hí­brido, cv.)

A study was done of the morphological behavior, specific leaf area (SLA) and leaf nitrogen concentration (LNC) of mulato grass (Brachiaria ruziziensis x B. brizantha) at different phenological stages (PS). Plant morphology (PM), growth rate (GR), height (H), SLA, and LNC were measured at seven-day intervals during a 21-week experimental period. Data were analyzed using the SAS GLM procedure in a completely randomized experimental design, with four replications and a regression analysis for each variable. PM varied significantly (P<0.05) in the different PS's. The highest leaf biomass was reached on d 102 after germination (5.7 +- 1.059 g DM plant-1). The highest GR was recorded at about wk 16 (2.44 +- 0.474 g DM plant-1 day-1). LNC attained the highest level at wk 4 (6.98%) and then declined until wk 21 (0.683%). LNC was positively correlated to SLA (R2=0.86971, P<0.001), and negatively correlated to plant height (R2=0.90231, P<0.001). Results suggest that as SLA increases, so does LNC, though it decreases as the plant matures. Plant height is negatively correlated to LNC.

Delayed greening of mountain birch leaves:Ecological and chemical correlates

Flushing leaves of deciduous trees can be exposed to high herbivore pressure. Young foliage provides high quality food for herbivores, and therefore herbivore pressure is much higher in young than mature foliage. Plants may escape the effects of herbivory in time by delaying leaf greening. We measured delayed greening as an intensity of redness in leaves in mountain birch at bud burst. Furthermore, we analyzed covariation of leaf redness with leaf growth and leaf consumption by the autumnal moth, Epirrita autumnata (Bkh.), a major defoliator of mountain birch. We also analyzed concentrations of amino acids, sugars and phenolic compounds of leaves. Leaf redness was positively associated with leaf growth, and consumption by E. autumnata indicated reduced leaf resistance against herbivores. Concentrations of protein-bound amino acids and gallotannins in young leaves were closely and positively correlated with leaf redness. Concentrations of glucose exhibited close positive correlations with leaf redness at the beginning of the season, whereas the correlation was negligible in mature leaves. Concentrations of other sugars increased with increasing degree of leaf redness towards the end of leaf development. This may be one reason for high leaf biomass losses in trees with delayed greening.

Organic matter technologies for integrated nutrient management in smallholder cropping systems of southern Africa

One of the biggest challenges in the tropics is to develop organic matter technologies which are adopted by the farmers. Technologies must be effective within farmer resource constraints, increase food production, reduce risk and enhance the soil fertility. Results from on-farm participatory research were used to quantify the effects of agronomic practices on soil resources. Agricultural productivity is primarily nitrogen (N) limited throughout Malawi, and sub-humid Zimbabwe. Tightening economic constraints faced by farmers in the region have reduced inorganic fertilizer inputs and necessitate increased reliance on biologically-fixed N and N cycling. Three components of organic matter technology were evaluated: (1) the effects of residue quality; (2) the role of deep rooting systems; and (3) tradeoffs between legumes grown for grain versus soil regeneration. Perennial systems investigated include improved fallows, intercropping, and biomass transfer. Annual systems include intercrops and rotations of cereals with legumes. The most promising non-food legumes were Tephrosia and Sesbania. Interestingly, high quality residues of perennial legumes were most effective at supplying N in the short to medium term, whereas low quality residues immobilised N. Low quality residues were problematic for smallholder farmers who need immediately available N. Challenges to adoption of perennial system technologies include establishment costs, resource competition and delayed benefits. Farmer adoption of annual grain legumes is promoted by the simultaneous production of food; however, those species which have a high N harvest index add little to no net N to the soil. Species that combine some grain yield with high root and leaf biomass, thus a low N harvest offer a useful compromise of meeting farmer food security concerns and improving soil fertility. Promising genotypes include Arachis, Cajanus, Dolichos and Mucuna spp. On-farm N budgets indicate that legumes with high quality residues and deep root systems are effective ways at improving nutrient cycling. Areas of future research priority for smallholder farms in southern Africa were identified, including technologies which combine inorganic and organic fertilizer and improve legume growth and establishment on degraded soils.

Persistent Suppression in Dwarf Birch after Release from Heavy Summer Browsing by Caribou

The Rivibre George Caribou Herd (RGCH), in northern Quebec, erupted during a few decades to culminate at approximately 800,000 animals by 1990. The RGCH appeared regulated by competition for summer forage. Repeated browsing and trampling reduced summer range productivity to 50% compared to ungrazed areas, lichens and dwarf birch having been mostly responsible for this reduction. A reconnaissance flight in 1991 and intensive field work the following two summers suggested that caribou use of dwarf birch leaves was light following the period of peak numbers. We hypothesized that birch stands would show signs of rapid and vigorous recovery in 1993-1994. We surveyed five heavily browsed birch stands, and two inaccessible, lightly browsed ones. Although utilization of birch leaves by caribou ranged between 0 and 6% during the 1994 growing season, heavily browsed stands exhibited no signs of rapid and strong recovery. Young plants did not abound in heavily browsed stands compared to lightly browsed ones, neither was wood accumulation faster in the former than in the latter. Stem density and height were similar in heavily browsed and lightly browsed stands. However, the leaf/wood ratio and the percent dry weight of leaves and wood were higher in lightly browsed than in heavily browsed stands, which resulted in approximately twice as high leaf biomass in lightly browsed than in heavily browsed stands. We hypothesized that previous intense caribou browsing caused a persistent suppression of dwarf birches, and that stems could not accumulate sufficient reserves during a growing season to deploy more leaves in the next spring in order to return the leaf/wood ratio to a normal value. We discuss the consequences of such a reaction of dwarf birch to herbivory on the demography of the RGCH.

Persistent Suppression in Dwarf Birch after Release from Heavy Summer Browsing by Caribou

The Rivière George Caribou Herd (RGCH), in northern Québec, erupted during a few decades to culminate at approximately 800,000 animals by 1990. The RGCH appeared regulated by competition for summer forage. Repeated browsing and trampling reduced summer range productivity to 50% compared to ungrazed areas, lichens and dwarf birch having been mostly responsible for this reduction. A reconnaissance flight in 1991 and intensive field work the following two summers suggested that caribou use of dwarf birch leaves was light following the period of peak numbers. We hypothesized that birch stands would show signs of rapid and vigorous recovery in 1993-1994. We surveyed five heavily browsed birch stands, and two inaccessible, lightly browsed ones. Although utilization of birch leaves by caribou ranged between 0 and 6% during the 1994 growing season, heavily browsed stands exhibited no signs of rapid and strong recovery. Young plants did not abound in heavily browsed stands compared to lightly browsed ones, neither was wood accumulation faster in the former than in the latter. Stem density and height were similar in heavily browsed and lightly browsed stands. However, the leaf/wood ratio and the percent dry weight of leaves and wood were higher in lightly browsed than in heavily browsed stands, which resulted in approximately twice as high leaf biomass in lightly browsed than in heavily browsed stands. We hypothesized that previous intense caribou browsing caused a persistent suppression of dwarf birches, and that stems could not accumulate sufficient reserves during a growing season to deploy more leaves in the next spring in order to return the leaf/wood ratio to a normal value. We discuss the consequences of such a reaction of dwarf birch to herbivory on the demography of the RGCH.