Highest Laccase Research Articles

Effect of phenolic compounds and tannin on growth and laccase activity of Fomes annosus

AbstractThe growth ofFomes annosus in liquid medium was inhibited by phenols which were oxidized by the fungus and/or induced high laccase acitivity. The effect of some of the tested phenols was different when they were applied to wood.The applicability of hydroquinone in spruce‐stems as protecting agent against invasion of Fomes annosus is discussed.

The phenoloxidases of the ascomycete Podospora anserina X. Electron microscopic studies on the structure of laccases I, II and III

Electron microscopy of three intracellular laccases from the Ascomycete Podospora anserina showed that the high molecular weight laccase I ( M r = 390 000) appears mainly as tetramers with the four subunits arranged at the corners of rectangles (13.3 nm × 8.5 nm). In size and shape the subunits of laccase I equal the monomeric molecules of laccase II ( M r = 70 000, 6.8 nm × 5.5 nm) and laccase III ( M r = 80 000, 7.0 nm × 5.8 nm). The dimensions of the monomeric laccases estimated from the electron micrographs are in good agreement with the hydrodynamic measurements of their molecular weight.

The phenoloxidases of the Ascomycete Podospora anserina

In order to learn the internal conditions for the production of the various phenoloxidases produced by the Ascomycete Podospora anserina the wild strain has been grown under controlled conditions in a fermenter for a period of 34 days. Samples were withdrawn at regular intervals and assayed for mycelial yield and intra- and extracellular phenoloxidase production. Maximal yield was obtained at the following age of the culture: Mycelial production 9 d, tyrosinase 4 d, the high molecular weight laccase I between 9 and 19 d. The low molecular weight laccases II and III, initially present in medium concentrations, dropped to an early minimum after 4 days, followed by an increase with a maximum in the late autolytic phase. The changes in the phenoloxidase spectrum and the antiparallel production curve for the high molecular weight against the low molecular weight laccases are discussed in relation to the earlier observed genetical and physiological control of phenoloxidase synthesis and in relation to the possibility of laccase I being composed of active subunits of low molecular weight laccases.

Production of High Laccase Yields in Cultures of Fungi

Production of High Laccase Yields in Cultures of Fungi