Highest Inhibition Efficiency Research Articles

Amphiphilic acrylic copolymers containing zwitterions: Modulation of hydrophilic unit and hydrophobic unit for antifouling

Amphiphilic polymers with surface zwitterion groups often show promising antifouling properties in marine environments. In this study, a series of amphiphilic copolymers containing zwitterion moieties (ACZ) were synthesized by the radical copolymerization of zwitterion 3-Triethylammonio-2-methacryloyloxypropane-1-sulfonate (TMS), lauryl methacrylate (LMA), methacrylic acid (MAA), and acrylic acid (AA). By regulating the amount of TMS moieties and hydrophobic LMA moieties, amphiphilic coatings with excellent antifouling properties was realized successfully. When the amounts of hydrophilic TMS and hydrophobic LMA were 5.0 mol% and 46.9 mol%, respectively, high antibacterial efficiencies of 94.8 %, 95.1 % and 95.2 % towards E. coli, S. aureus and Pseudoalteromonas, respectively, were obtained. In addition, high inhibition efficiencies towards P. tricornutum and C. vulgaris of 80.4 % and 68.4 %, respectively, could be achieved. Furthermore, the ACZ samples delivered excellent antifouling performance when immersed in a marine environment for 147 days. This study encourages the development of new amphiphilic materials for prevention of marine fouling.

Efficacy of Andrographis paniculata leaf extract as a green corrosion inhibitor for mild steel in concentrated sulfuric acid: Experimental and computational insights

The rising demand for eco-friendly corrosion inhibitors has intensified efforts to find sustainable alternatives to hazardous chemical inhibitors widely used in industry. Mild steel, although commonly utilized in acidic environments, is prone to significant corrosion, necessitating effective solutions to prevent structural degradation. This study investigates the potential of Andrographis paniculata leaf extract (APLE) as a sustainable, green corrosion inhibitor for mild steel in concentrated sulfuric acid, a condition representative of harsh industrial environments. Using electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PDP) methods, APLE demonstrated a high inhibition efficiency of 95.14% at a concentration of 4000 ppm, effectively reducing both anodic and cathodic corrosion reactions. Scanning electron microscopy (SEM) revealed a protective layer formation on the steel surface, significantly reducing corrosion. The adsorption of APLE followed the Langmuir isotherm, indicating monolayer coverage, while quantum chemical calculations validated APLE's electron-donating capability, enhancing surface stability through physisorption. These findings underscore APLE as a viable, eco-friendly alternative to conventional inhibitors, offering promising applications in various acidic environments.

Ultra-tough, strong and transparent bio-based waterborne polyurethanes with exceptional anti-corrosion properties

Waterborne polyurethane (WPU) is increasingly favored because of its affordability, eco-friendliness, and water-based storage convenience. However, the inclusion of hydrophilic groups can diminish its mechanical strength and water resistance. Herein, a range of bio-based waterborne polyurethanes were synthesized by using economical and sustainable poly(trimethylene ether) glycol (PO3G), isophorone diisocyanate (IPDI), and poly(propylene glycol) (PPG) as raw materials. They exhibited superior mechanical properties, transparency, and corrosion resistance. We explored the effect of PO3G content on the properties of these bio-based WPU emulsions and films. The findings revealed that films with over 30 % PO3G content demonstrated a high tensile strength (above 10 MPa) and maintained a high elongation at break (above 4000 %), matching or surpassing existing bio-based WPU systems. The corrosion resistance of these films was also exceptional, with a high inhibition efficiency (above 99.97 %). This research introduces a new approach for creating high-performance bio-based WPUs with promising applications in coatings, leather, and biomedical materials.

Novel Schiff base derivatives: Enhanced corrosion protection for copper in NaCl solutions – Electrochemical insights and theoretical analysis

This study investigates the corrosion inhibition properties of two recently synthesized Schiff bases, namely 4-bromo-2-(((2-((2-hydroxyethyl)amino)ethyl)imino)methyl)phenol (BHA) and phenyl-2-(((2-((2-hydroxyethyl)amino)ethyl)imino)methyl)phenol (PHA), for copper in a 3.5 wt% NaCl solution. The novelty of this work lies in the use of these Schiff bases, which have not been previously studied in this context, thereby providing new insights into their potential as effective copper corrosion inhibitors. The inhibition performance of the Schiff bases was evaluated using potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS). The potentiodynamic polarization results demonstrated high corrosion inhibition efficiencies of 85.16 % for PHA and 82.78 % for BHA at a concentration of 1 mM, indicating a mixed-type inhibition process with cathodic predominance. The adsorption of these inhibitors on the copper surface was found to follow the Langmuir adsorption isotherm, and the adsorption constants (Kads) and Gibbs free energies (ΔG*ads) were calculated. Quantum chemical calculations, in conjunction with Monte Carlo (MC) and molecular dynamics (MD) simulations, provided further insight into the adsorption mechanisms and supported experimental findings, thus offering a comprehensive understanding of the inhibitors’ efficacy.

N, S-Codoped Carbon Quantum Dots with High Inhibition Efficiency: Implications for Corrosion Mitigation of Carbon Steel in Acidic Environments.

The environmental friendliness, economic feasibility, and high efficiency of carbon quantum dots (CQDs) render them as highly promising candidates for corrosion inhibitors. The present study proposed the fabrication of nitrogen- and sulfur-codoped CQDs via an one-step hydrothermal method using l-cysteine and 4-aminosalicylic acid as precursors. The structure, particle size, and surface ligands of the prepared CQDs were determined through spectroscopy and transmission electron microscopy characterization. Subsequently, the inhibition performance of the CQDs on carbon steel in a 0.5 M sulfuric acid solution was evaluated through weight loss measurement, electrochemical methods, and surface analysis. The CQDs exhibited remarkable inhibition efficiencies of 97.9% at 293 K and 98.9% at 313 K, with a concentration of 150 ppm. In addition, the obtained CQDs demonstrated a combined physisorption and chemisorption adsorption behavior, which complied with the Langmuir adsorption isotherm. These findings provide insight into the inhibition mechanism and highlight the potential of codoped CQDs for corrosion mitigation applications in acidic environments.

Corrosion Inhibition of AZ31-xLi (x = 4, 8, 12) magnesium alloys in sodium chloride solutions by aqueous molybdate

Corrosion of lithium-containing AZ31 magnesium alloys AZ31-xLi (x = 4, 8, and 12 wt%) has been examined in 0.05 M NaCl solution with and without 10–150 mM of Na2MoO4 inhibitor. Potentiodynamic polarization, electrochemical impedance spectroscopy (EIS), and dynamic electrochemical impedance spectroscopy (DEIS) measurements were used to correlate the phase composition and microstructure of the alloys with their corrosion propensity and effectiveness of the molybdate inhibitor, giving high inhibition efficiency (>85%) at concentrations higher than ca. 35 mM. Post-corrosion microstructure, Raman, and X-ray photoelectron spectroscopy analyses allowed to provide the inhibition mechanism of AZ31-xLi alloys by molybdate ions.

DFT, experimental and optimization studies on the corrosion inhibition of aluminium in H2SO4 with danacid as inhibitor

The inhibitive action of expired danacid toward the dissolution of aluminium in 1 M H2SO4 was examined in this study via gravimetric, electrochemical, and theoretical method via density functional theory (DFT) in addition to studying the surface morphology and functional groups with scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). Optimization of inhibition parameters was performed with response surface methodology (RSM). FTIR result indicates the presence of heteroatoms in the inhibitor while further study by gas chromatography mass spectrometry (GC-MS) revealed the presence of octadecane, carbonic acid, eicosyl vinyl ester, dotriacontane, 1-chloro- Hexadecane, 1-chloro-octadecane, 1-fluoro-Tetradecane, n-hexadecanoic acid, pentafluoropropionic acid, tetradecyl ester, linoelaidic acid, among others. The inhibition efficiency of expired danacid increased with increase in danacid's concentration and reduced with rise in temperature. Potentiodynamic polarization (PDP) result portrayed the expired danacid as a mixed-type inhibitor (M-TI) with the highest inhibition efficiency (IE) of 94.06 % while RSM optimization gave an IE of 94.24 %. DFT analysis revealed further parameters that support the inhibitory action of danacid. Adsorption studies showed that Langmuir isotherm gave the best fit to the experimental data. Artificial neural network (ANN) modeling further improved on the RSM predicted results. The experimental, electrochemical and optimization results show consistency with each other.

Sonophotopythochemical Functionalization of Graphene Oxide - Al - Zn Bimetal Nanocomposite for Corrosion Inhibition

The corrosion performance of steel in the marine environment has been a primary concern of engineers and garnered significant interest due to its industrial significance. To address this concern, the incorporation of green corrosion inhibitors as coating materials in mild steel has been extensively studied recently. This paper explores the synthesis of graphene doped with bimetal aluminum-zinc (GO-Al-Zn) nanocomposites via sonophoto-phytochemical functionalization using Chayote (Sechium edule) leaf extract as the doping agent to produce a corrosion inhibitor. The synthesized nanocomposites were characterized using FTIR, SEM-EDS, XRD, and TEM. The optimal nanocomposite, with a 55% Al - 45% Zn ratio, demonstrated successful bio-reduction, good dispersion, reduced particle size, and a rhombohedral crystal structure. When incorporated into an epoxy coating and applied to mild steel, the GO - 55% Al - 45% Zn coating achieved a high corrosion inhibition efficiency of 98.06% (gravimetric method) and 98.45% (electrochemical method) in 3.5 wt% NaCl solution. This study highlights the promising potential of GO - 55% Al - 45% Zn nanocomposite as an eco-friendly corrosion inhibitor. Future research should explore optimizing the functionalization process and exploring long-term environmental stability.

Inhibitor_Mol_VAE: a variational autoencoder approach for generating corrosion inhibitor molecules

Deep learning-based generative modeling demonstrates proven advantages as an effective approach in molecular discovery. This study introduces a generative-network based method called Inhibitor_Mol_VAE, which uses a variational autoencoder model to generate corrosion inhibitor molecules with targeted inhibition efficiency. We first evaluate the model’s ability to reconstruct molecules. Then, we assess the model’s ability to generate new inhibitor molecules using physiochemical properties (including MolWt, LogP, Vdw_volume, and Electronegativity). New molecules with high inhibition efficiencies at low concentrations, such as [ethoxy(methoxy)phosphoryl]-phenylmethanol and (alpha-methylamino-benzyl)-phosphonsaeure-monoaethylester are successfully discovered.

Synthesis, Characterization, Theoretical, and evaluation of Eco-Friendly benzimidazolylmethyl-pyrazole carboxylate Schiff Bases corrosion inhibitors for mild steel

In this study, benzimidazolylmethyl-pyrazole carboxylate derivatives were synthesized via a 1,3-dipolar cycloaddition reaction and characterized using IR, HRMS and NMR spectroscopy. Three derivatives, namely ethyl 5-((3-(cyclohex-1-en-1-yl)-2-oxo-2,3-dihydro-1H-benzo[d]imidazol-1-yl)methyl)-1-phenyl-1H-pyrazole-3-carboxylate (Ph-BPC), ethyl 5-((3-(cyclohex-1-en-1-yl)-2-oxo-2,3-dihydro- 1H-benzo[d]imidazol-1-yl)methyl)-1-(p-tolyl)-1H-pyrazole-3-carboxylate (CH3Ph-BPC), and ethyl 1-(4-chlorophenyl)-5-((3-(cyclohex-1-en-1-yl)-2-oxo-2,3-dihydro-1H-benzo[d]imidazol-1-yl)methyl)-1H-pyrazole-3-carboxylate (ClPh-BPC), were evaluated as corrosion inhibitors for mild steel (MS) in a 1 M HCl solution. Potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) measurements revealed high inhibition efficiencies of 98.5 %, 98.2 %, and 97.7 % for ClPh-BPC, Ph-BPC, and CH3Ph-BPC, respectively, at a concentration of 10-4 M. Scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) and X-ray diffraction (XRD) analyses confirmed the formation of protective surface layers, significantly reducing corrosion rates. Thermodynamic analysis indicated that the adsorption of these inhibitors follows the Langmuir isotherm model with adsorption energies of −50.12 kJ/mol for ClPh-BPC, −50.52 kJ/mol for Ph-BPC, and −51.62 kJ/mol for CH3Ph-BPC, suggesting chemisorption. Computational studies, including Monte Carlo (MC), density functional theory (DFT), and molecular dynamics (MD) simulations, showed strong agreement with experimental results, further validating the adsorption behavior and corrosion inhibition mechanisms of these compounds.

Prominent Neuroprotective Potential of Indole-2-N-methylpropargylamine: High Affinity and Irreversible Inhibition Efficiency towards Monoamine Oxidase B Revealed by Computational Scaffold Analysis

Background: Monoamine oxidases (MAO) are flavoenzymes that metabolize a range of brain neurotransmitters, whose dysregulation is closely associated with the development of various neurological disorders. This is why MAOs have been the central target in pharmacological interventions for neurodegeneration for more than 60 years. Still, existing drugs only address symptoms and not the cause of the disease, which underlines the need to develop more efficient inhibitors without adverse effects. Methods: Our drug design strategy relied on docking 25 organic scaffolds to MAO-B, which were extracted from the ChEMBL20 database with the highest cumulative counts of unique member compounds and bioactivity assays. The most promising candidates were substituted with the inactivating propargylamine group, while further affinity adjustment was made by its N-methylation. A total of 46 propargylamines were submitted to the docking and molecular dynamics simulations, while the best binders underwent mechanistic DFT analysis that confirmed the hydride abstraction mechanism of the covalent inhibition reaction. Results: We identified indole-2-propargylamine 4fH and indole-2-N-methylpropargylamine 4fMe as superior MAO-B binders over the clinical drugs rasagiline and selegiline. DFT calculations highlighted 4fMe as more potent over selegiline, evident in a reduced kinetic requirement (ΔΔG‡ = −2.5 kcal mol−1) and an improved reaction exergonicity (ΔΔGR = −4.3 kcal mol−1), together with its higher binding affinity, consistently determined by docking (ΔΔGBIND = −0.1 kcal mol−1) and MM-PBSA analysis (ΔΔGBIND = −1.5 kcal mol−1). Conclusions: Our findings strongly advocate 4fMe as an excellent drug candidate, whose synthesis and biological evaluation are highly recommended. Also, our results reveal the structural determinants that influenced the affinity and inhibition rates that should cooperate when designing further MAO inhibitors, which are of utmost significance and urgency with the increasing prevalence of brain diseases.

Effects of CTAB (Cetyltrimethylammonium Bromide) and Betaine as Corrosion Inhibitors on the Galvanic Corrosion of Cu Coupled with Au on Print Circuit Board in Etching Solution

This study investigates the suppression of galvanic corrosion between copper and gold using cetyltrimethylammonium bromide (CTAB) and betaine as inhibitors. When copper is electrically connected to gold in PCB etching solutions, the substantial difference in their electrochemical potentials leads to the accelerated corrosion of copper, posing severe reliability risks. To mitigate this, we systematically investigated the galvanic corrosion inhibition properties of CTAB and betaine. Through comprehensive electrochemical analyses, it was found that the galvanic corrosion current density of copper, initially at 3.26 mA/cm2, decreased significantly to 0.251 mA/cm2 with 0.9 mM CTAB, indicating an inhibition efficiency of 92.3%. Furthermore, betaine, at a concentration of 0.1 mM, demonstrated an even higher inhibition efficiency, reducing the corrosion current to 0.03 mA/cm2, achieving a 99.1% inhibition rate. These findings provide strong evidence that CTAB and betaine are highly effective in suppressing galvanic corrosion in copper–gold systems, thereby enhancing the long-term performance and reliability of PCBs in electronic applications.

High-throughput screening of green amino acid and surfactant mixtures with high corrosion inhibition efficiency: Experimental and modelling perspectives

A high-throughput screening method is developed for rapid evaluation of corrosion inhibitors. By employing a fluorometric technique, this method quickly assesses the concentration of iron ions converted from corrosion products in the reaction pool to rapidly evaluate the corrosion inhibition performance of amino acid and surfactants. Utilizing this high-throughput screening platform, the corrosion inhibition effects of L-cysteine mixed with quaternary ammonium salts of different alkyl chain length were evaluated, and their optimal mixing ratios were determined. Through corrosion experiments and molecular simulations, the synergistic mechanisms of the inhibitor mixtures are revealed.

New Benzimidazole derivatives as efficient organic inhibitors of mild steel corrosion in hydrochloric acid medium: Electrochemical, SEM/EDX, MC, and DFT studies

The corrosion of mild steel (MS), a widely used material in industry, represents a significant global challenge. In order to extend the durability of equipment, the use of corrosion inhibitors proves to be an effective solution, particularly in corrosive environments. In this research, two newly synthesized benzimidazole derivatives, namely 1-(cyclohex-1-en-1-yl)-3-[(3-phenyl-1,2-oxazol-5-yl)methyl]-2,3-dihydro-1H-1,3-benzodiazol-2-one (Benz1) and 1-(cyclohex-1-en-1-yl)-3-[(3-phenyl-4, 5-dihydro-1,2-oxazol-5-yl)methyl]-2,3-dihydro-1H-1,3-benzodiazol-2-one (Benz2), were evaluated as corrosion inhibitors for MS in 1 M hydrochloric acid solution. This study was conducted using both experimental methods and computational approaches. Potentiodynamic polarization diagrams (PDP) indicate that, depending on concentration effects, Benz1 and 2 function as mixed-type inhibitors, with a predominance of cathodic inhibition. The results of electrochemical impedance spectroscopy (EIS) show that the compounds tested offer high inhibition efficiencies of 96.32 % and 98.30 % at 10−⁴ M for Benz1 and 2, respectively. Inhibitor adsorption follows the Langmuir isotherm and involves chemisorption. SEM/EDX analyses confirm the presence of a protective film on the metal. In addition, DFT and Monte Carlo simulations reveal significant adsorption of the compounds on the MS surface, underlining their high potential as effective corrosion inhibitors.

Electrochemical behavior of mild steel coated with long alkyl-chain benzothiazole derivatives in acid solution: Effect of aliphatic chain length

Mild steel surfaces were coated with four different benzothiazole derivatives with long alkyl chain, namely 2-(heptyl)-benzothiazole (HEBT), 2-(tridecyl)-benzothiazole (TDBT), 2- (pentadecyl)-benzothiazole (PDBT), and 2-(heptadec-8-en-1-yl)benzothiazole (HDBT) from their respective chloroform solutions. Electrochemical experiments were conducted with coated and uncoated mild steel samples exposed to 1 M aqueous HCl at temperatures between 20°C to 50°C and at different immersion times from 6 h to 48 h. TDBT behaves as the best corrosion inhibitor among the four (more than 99 % efficiency at 40°C) and the order of inhibition efficiency follows the trend: TDBT>HDBT>PDBT>HEBT. This reflects the inhibition efficiency to increase with chain length initially, but decreases after a threshold. HDBT, in-spite of having longest aliphatic chain, exhibits better inhibitory performance over PDBT. This is attributed to the presence of unsaturation in HDBT. TDBT coating maintains high corrosion inhibition efficiency even after the 24 h of exposure (above 98 %). Occurrence of spatial hindrance during adsorption of BT derivatives with long aliphatic chain has been manifested by MD simulation.

Chitosan grafted with gallic acid and cerium dioxide hybrid nanocomposites as environmentally friendly corrosion inhibitors for mild steel: An experimental and computational study

Chitosan grafted with gallic acid (CS-GA), along with CS-GA doped with CeO2 nanoparticles (CS-GA-CeO2) were synthesized as novel environmentally friendly mild steel corrosion inhibitors. The formation of these derivatives was confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), hydrogen nuclear magnetic resonance spectroscopy (1H NMR), and thermal analysis (TGA). Based on potentiodynamic polarization curves (PDP) measurements, the inhibitors acted primarily as hybrid inhibitors, while following the Langmuir adsorption theory model. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy(XPS) and 3D surface profiles, confirmed that CS-GA-CeO2 adsorbed on the mild steel forming a protective layer thus preventing the invasion of corrosive media. The corrosion protection mechanism of chitosan derivatives was investigated by molecular dynamics simulations. Electrochemical measurements were used to investigate the corrosion inhibition by CS-GA and CS-GA-CeO2 on mild steel in a 3.5 % NaCl solution. At room temperature, the highest inhibition efficiency (93.58 %) was achieved at 200 ppm CS-GA-CeO2. Modified chitosan nanocomposites were confirmed as promising corrosion inhibitors.

Strain effects on corrosion inhibition in stress corrosion of tubing steel

In this investigation, we explored the corrosion inhibition mechanism of an imidazoline quaternary ammonium salt (IQA) on J55 steel in simulated annulus environment through a series of experiments, including electrochemical testing, stress corrosion immersion experiments, and hydrogen permeation testing. Our findings reveal that IQA functions as a mixed-type inhibitor, exerting its inhibitory action through chemical adsorption. Notably, it exhibits a stronger inhibitory effect on the anodic dissolution reaction compared to the cathodic hydrogen evolution reaction. Despite the minor influence of tensile plastic strain on the average inhibition efficiency, it notably exacerbates pitting and initiates stress corrosion cracking. This underscores the limitation of average inhibition efficiency in accurately assessing IQA's efficacy against stress corrosion. Additionally, hydrogen permeation experiments and electrochemical testing demonstrate that plastic strain diminishes IQA's inhibitory effect on the cathodic hydrogen evolution reaction, facilitating hydrogen diffusion into the steel substrate and thereby exacerbating stress corrosion in J55 steel. Consequently, at low IQA inhibitor concentrations (as in this study, 12.5 mg L−1), despite high average inhibition efficiency, it proves ineffective in mitigating stress corrosion.

Synthesis, characterization, theoretical, and experimental evaluation of novel imidazolone − based compounds as eco-friendly corrosion inhibitors for mild steel

This study investigates the corrosion inhibition properties of two newly synthesized namely (E)-2-(2-benzylidenehydrazineyl)-5,5-diphenyl-3,5-dihydro-4H-imidazol-4-one denoted Ph-DDI and (E)-2-(2-(4-methylbenzylidene)hydrazineyl)-5,5-diphenyl-3,5-dihydro-4H-imidazol-4-one denoted CH3Ph-DDI, on mild steel (MS) in a 1 M HCl solution. These compounds demonstrated high inhibition efficiencies of 98.3 % and 98.7 %, respectively. Structural characterization was performed using FT-IR, 1H NMR, 13C NMR, and HRMS-ESI. Theoretical evaluations indicated high reactivity and potent inhibition capacity. Electrochemical tests confirmed a concentration-dependent inhibition effectiveness up to 328 K. Adsorption studies suggested that the compounds displace water molecules to form an adsorbed protective layer. Microscopy analysis provided insights into the corrosion inhibition mechanisms, confirming the formation of protective layers and iron/inhibitor complexes. Further molecular structure analysis using Monte Carlo (MC) simulations and density functional theory (DFT) calculations elucidated the structural features contributing to the compounds’ effective corrosion inhibition properties.

In situ mildly synthesized CuZnAl LDHs nanofilm with long-lasting corrosion resistance and self-healing properties induced by corrosive Cl– ions

A smart means to achieve the harmless treatment of corrosive Cl– have been proposed for protecting brass. In this method, intelligent self-healing nanofilms based on the interlayer anion exchange properties of LDHs in situ grow on brass surfaces. The Cl– ions replace the BTA– loaded between LDHs interlayers to induce self-healing, as the binding energy of Cl– is lower than that of BTA–, obtained from theoretical calculations. The simultaneous application of electrochemistry and digital holography technologies confirmed that the constructed film has high instantaneous corrosion inhibition efficiency (99.9 %), long-lasting corrosion resistance, and self-healing ability (50 days).

Nifuroxazide Prevents Chikungunya Virus Infection Both In Vitro and In Vivo via Suppressing Viral Replication.

Chikungunya virus (CHIKV) is a reemerging arbovirus causing disease on a global scale, and the potential for its epidemics remains high. CHIKV has caused millions of cases and heavy economic burdens around the world, while there are no available approved antiviral therapies to date. In this study, nifuroxazide, an FDA-approved antibiotic for acute diarrhea or colitis, was found to significantly inhibit a variety of arboviruses, although its antiviral activity varied among different target cell types. Nifuroxazide exhibited relatively high inhibitory efficiency in yellow fever virus (YFV) infection of the hepatoma cell line Huh7, tick-borne encephalitis virus (TBEV) and west nile virus (WNV) infection of the vascular endothelial cell line HUVEC, and CHIKV infection of both Huh7 cells and HUVECs, while it barely affected the viral invasion of neurons. Further systematic studies on the action stage of nifuroxazide showed that nifuroxazide mainly inhibited in the viral replication stage. In vivo, nifuroxazide significantly reduced the viral load in muscles and protected mice from CHIKV-induced footpad swelling, an inflammation injury within the arthrosis of infected mice. These results suggest that nifuroxazide has a potential clinical application as an antiviral drug, such as in the treatment of CHIKV infection.