Highest Folate Content Research Articles

Effects of different spices on the bioactive compounds, antioxidant activity, and sensory qualities of kale crisps

SummaryThe study investigated the effect of different spices–cardamom, chilli, ginger, and turmeric on the bioactive compounds, antioxidant activity, colour, and sensory attributes of kale crisps. Specifically, the antioxidant capacity was ranked as follows: kale crisps with turmeric > chilli ≈ ginger > cardamom ≈ control crisps. The analysis using HPLC‐MS/MS focused on 15 specific compounds. Kale crisps contained predominantly chlorogenic and isochlorogenic acids, with turmeric crisps having the highest levels. Turmeric kale crisps exhibited the best sensory properties for overall appearance and crispness. The study found that kale crisps have a high folate content, with ginger crisps containing the highest amounts. Cardamom kale crisps preserved high folate content but had a strong aroma and bitter flavour. The study found lightness (L*) positively correlated with aroma desirability, while redness (a*) negatively correlated with flavour desirability. The chilli kale crisps had a strong flavour and lost their green colour the most, resulting in a lower panel ranking despite high antioxidant potential. This study indicates that kale crisps, especially those with turmeric, may represent a valuable source of bioactive compounds and functional properties. Sensory evaluation indicated their high levels of acceptability.

An improved folate stable isotope dilution assay of unexploited food sources from Brazil.

Brazil has a diverse plant community, including underutilized non-conventional food crops (PANCs), which have the potential to be a rich source of food and contribute to food security. For assessing the folate content in a range of Brazilian PANCs, we extended the validation of an existing stable isotope dilution assay (SIDA) for the stably 13C-labelled 10-formyl-Pte[13C5]Glu (10-CHO-Pte[13C5]Glu). The SIDA method with an enzymatic treatment, purification step, and an LC-MS/MS measurement was validated regarding linearity, precision, LoD/LoQ, and recovery for 10-CHO-PteGlu. After successful validation, the study of some underutilized Brazilian non-conventional fruits and leaves from the São Paulo State University campus revealed them as an important source of folates. It provided the first insights into the folate content of unexploited food sources from Brazil. Pequi had the highest folate content among the fruits studied, with mean values of 333 μg/100 g based on fresh weight (FW). The analysis also shows that different cultivars of fruit or fruits from different growing locations have a high variability in folate content or other nutritional factors. In most fruits, the main vitamer was 5-CH3-H4folate, but jenipapo and taioba showed the highest content of 10-CHO-PteGlu with 28.22 μg/100 g (FW) in jenipapo peel and 75.64 μg/100 g (FW) in the taioba leaves. Thus, this study also provides results on the importance of the folate vitamer 10-CHO-PteGlu contributing to the total folate content.

“Health Benefits of Beetroot Juice” - A Review

A major problem for adolescent girls is malnutrition and anaemia. Inadequate nutrition during adolescence has the potential to have a serious negative influence on the reproductive years and beyond. The likelihood that Indian girls would acquire anaemia is doubled since they typically marry and have children before their growth spurt is complete (Chatterjee, 2008). Because of its high nitrate concentration and beta carotene content (19690 mcg/100g), beetroot juice with jaggery aids in the mobilisation of stored iron and increases haemoglobin levels in anaemic people. With its potential to stabilise blood pressure, beetroot juice is used to treat anxiety. In persons with diabetes, it is believed to improve blood flow, lower blood pressure, and improve athletic performance. Because beetroot juice has a diuretic effect and is occasionally combined with Amla juice, eating beets is recommended in cases of gonorrhoea. Heart disease, diabetes, and obesity are all decreased by beetroot juice. Jaggery's high iron and folate content assists adolescent girls with a number of menstruation issues, including easing stomach pain during periods and preventing anaemia by maintaining a proper volume of blood cells. Increased blood counts, better circulation, and a rise in the capacity of erythrocytes to carry oxygen are all results of beetroot juice's assistance in the absorption of iron.

Folate Enrichment of Whole-Meal Spaghetti Using Durum Wheat Debranning Fractions.

Durum wheat debranning fractions (fine and coarse bran) were obtained and included as an ingredient in the formulation of whole-meal spaghetti to study their chemical-nutritional characteristics, in particular folate levels and sensorial properties. Experimental raw pasta had a higher folate content (40.5 µg/100 g) than commercial whole-meal pasta (28.3 µg/100 g), meeting the requirements for the health claim on folate (Reg. EU 432/2012) and for the nutritional claim on dietary fiber. After cooking, folate retention in pasta formulated with coarse bran was 80% and scored an overall "good" sensorial acceptability. Results indicate that whole-meal pasta formulated with folate-rich debranning fractions may represent a natural functional food that, integrated into the diet, could improve the health status of the population.

Health-Promoting Ingredients in Goat's Milk and Fermented Goat's Milk Drinks.

The present study aimed to determine the content of health-promoting compounds, and fatty acids, with particular emphasis on the content of cis9trans11 C18:2 (CLA) acid, selected minerals, folates in organic and commercial goat's milk and fermented goat's milk drinks. The analyzed milk and yoghurts had various contents of particular groups of fatty acids, CLA, minerals, and folates. Raw organic goat's milk had a significantly (p < 0.05) higher content of CLA (3.26 mg/g fat) compared to commercial milk (2.88 mg/g fat and 2.54 mg/g fat). Among the analyzed fermented goat's milk drinks, the highest CLA content (4.39 mg/g fat) was determined in commercial natural yoghurts, while the lowest one was in organic natural yoghurts (3.28 mg/g fat). The highest levels of calcium (1322.9-2324.4 µg/g), phosphorus (8148.1-11,309.9 µg/g), and copper (0.072-0.104 µg/g) were found in all commercial products and those of manganese (0.067-0.209 µg/g) in organic products. The contents of the other assayed elements (magnesium, sodium, potassium, iron, and zinc) did not depend on the production method, but only on the product type, i.e., the degree of goat's milk processing. The highest folate content in the analyzed milks was found in the organic sample (3.16 µg/100 g). Organic Greek yoghurts had a several times higher content of folates, reaching 9.18 µg/100 g, compared to the other analyzed fermented products.

Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.)

Vitamin B9, particularly folic acid, is an essential molecule for human health. Wheat flour is one of the major sources of calorie intake by humans. The selection of folate-rich genotypes in wheat breeding can enhance the natural folate value in the daily diet. This study used a precise, high-performance liquid chromatography (HPLC) assay to analyze folate content in a 262-accession Chinese wheat mini-core collection (MCC) grown under three environments. Four folate derivatives in grains including tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-CH3-THF), 5-formyltetrahydrofolate (5-CHO-THF), and 5,10-methenyltetrahydrofolate (5,10-CH+THF) were considered. An association analysis of water regimes, accession types, released years, geographical origin, and agronomic traits with folate content was conducted for the first time. There was a large amount of variation in folate content in the analyzed accessions, with genotype identified as the main influencing factor. Total folate content was significantly correlated with the content of the four MCC derivatives under the three environments. 5-CH3-THF and 5-CHO-THF were the most abundant among the four folate derivatives and were positively correlated with high folate content. The 12 accessions with the highest folate content showed an average of more than 80 μg/100 g. The analysis demonstrated that this Chinese wheat had not undergone extensive selection for folate content during breeding, which is unrelated to the geographical origin, accession types, winter/spring types, and grain colors of wheat. The content of THF, 5-CH3-THF, and 5,10-CH+THF was significantly negatively correlated with grain width, grain thickness, and thousand kernel weight. A relatively weak negative relationship manifested between folate contents and flowering date, whereas no significant correlation with tiller number, grain number per spike, maturity date, height, and spike length was detected. The investigation benefits wheat breeders for folate enhancement.

Characterizing the extent human milk folate is buffered against maternal malnutrition and infection in drought‐stricken northern Kenya

AbstractObjectivesFolate is an essential nutrient fundamental to human growth and development. Human milk maintains high folate content across the maternal folate status range, suggesting buffering of milk folate with prioritized delivery to milk at the expense of maternal depletion. We investigated whether and how the extent of this buffering may diminish under prolonged nutritional and/or disease stress, while taking into consideration infants' varying vulnerability to malnutrition‐related morbidity/mortality.MethodsA cross‐sectional study analyzed milk specimens from northern Kenyan mothers (n = 203), surveyed during a historic drought and ensuing food shortage. Multiple regression models for folate receptor‐α (FOLR1) in milk were constructed. Predictors included maternal underweight (BMI &lt; 18.5), iron‐deficiency anemia (hemoglobin &lt;12 g/dl and dried‐blood‐spot transferrin receptor &gt;5 mg/L), folate deficiency (hyperhomocysteinemia, homocysteine &gt;12 or 14 μmol/L), inflammation (serum C‐reactive protein &gt;5 mg/L), infant age and sex, and mother‐infant interactions.ResultsIn adjusted models, milk FOLR1 was unassociated with maternal underweight, iron‐deficiency anemia and inflammation. FOLR1 was positively associated with maternal folate deficiency, and inversely associated with infant age. There was interaction between infant age and maternal underweight, and between infant sex and maternal folate deficiency, predicting complex changes in FOLR1.ConclusionsOur results suggest that mothers buffer milk folate against their own nutritional stress even during a prolonged drought; however, the extent of this buffering may vary with infant age, and, among folate‐deficient mothers, with infant sex. Future research is needed to better understand this variability in maternal buffering of milk folate and how it relates to folate status in nursing infants.

Folate content of a staple food increased by fermentation of a cereal using selected folate-producing microorganisms

Folate deficiencies are widespread in Africa due to predominantly cereal-based diets. The objective of this work was to test the feasibility of using folate-producing microorganisms to increase folate content of tef injera, a traditional Ethiopian fermented staple food. To this end, a strain of Lactobacillus plantarum previously isolated from fermented tef batter and a commercial Saccharomyces cerevisiae were used alone and in combination to prepare injera. Ten successive fermentations using backslopping from the fermented batter prepared with L. plantarum inoculation were performed to mimic the traditional backslopping. The highest folate content was obtained with S. cerevisiae (53.5 μg/100 ​g fresh material). All the combinations were efficient and could cover up to 22 % of the recommended nutrient intakes. All injera prepared with selected inoculums were preferred by sensory panelists to the traditional one. This work demonstrates the possibility to increase folate intake using folate-producing microorganisms in the conditions normally encountered in households.

Physicochemical Properties and Nutrient Content of Tempe Flour Enriched Eel Flour

AIM: This study aims to evaluate the nutritional content and physicochemical aspect of the combination of eel and tempe flour. METHODS: Samples of swamp eel and tempe flour were combined in a ratio of 1:1 (A), 1:2 (B), 1:3.5 (C), and 1 kilogram of Asian swamp eel meat without additional tempe (D), respectively. Homogenization was done using a sinmag planetary mixer. The sample analysis was performed at the SIG Laboratory (PT. Saraswanti Indo Genetech) on November 16, 2021. The samples were analyzed triple (triplicates). Statistical analysis was performed using GraphPad Prism version 9.3.0 for Mac OS. Variables were analyzed using multivariate ANOVA with 95% CI (0.05). RESULTS: There was a significant difference between Samples A, B, C, and D for every proximate analysis parameter (p = 0.000) p &lt; 0.05. The results showed a significant difference in the unsaturated fatty acid parameter between the groups (p &lt; 0.001). Sample C has the highest B9 (folic acid) content compared to other samples (1258.53 ± 1.39 mcg/100 g) and a significant difference was found between samples (p &lt; 0.0001). CONCLUSION: Tempe flour enriched eel flour can act as a source of folate, due to its high folate content. Tempe flour enriched eel flour could be used as a flour mixture in any food products that require flour to increase folate content.

Natural variation of folate content in cowpea (Vigna unguiculata) germplasm and its correlation with the expression of the GTP cyclohydrolase I coding gene

Cowpea (Vigna unguiculata) is one of the most important sources of vitamins, protein, and minerals for Africa, Latin America and Asia. In addition, grains are a rich source of folates. Folates are cofactors for the one-carbon transfer reactions needed for many biochemical processes. Their deficiency is associated with several diseases. In this study, we investigated variation in total folate levels in cowpea genotypes from Northern Brazil, Western Africa and Southwestern Asia and the correlation with VugchI gene expression. Results revealed that VugchI transcripts were observed in all studied developmental stages [10-, 15-, 20- and 25-days post anthesis (dpa)], reaching a maximum at 20 dpa. Folate content ranged from 780.7–177.2 μg/100 g of dried seeds, and the highest amounts were observed in traditional varieties cultivated in Nigeria and Northern Brazil. A statistically significant correlation was observed between the amounts of total folate and the expression of the VugchI gene at 20 dpa. Data showed a large variation in the total folate content that could be exploited by breeding programs to generate higher-folate varieties. It would be important to combine both high folate content and agriculturally relevant traits, which would lead farmers to adopt modern varieties enriched with folates.

Changes in the Folate Content and Fatty Acid Profile in Fermented Milk Produced with Different Starter Cultures during Storage.

The application of bacterial cultures in food fermentation is a novel strategy to increase the “natural” levels of bioactive compounds. The unique ability of lactic acid bacteria (LAB) to produce folate, B vitamins, and conjugated linolenic acid cis9trans11 C18:2 (CLA) during cold storage up to 21 days was studied. Although some species of LAB can produce folates and other important nutrients, little is known about the production ability of yogurt starter cultures. Pasteurized milk samples were inoculated with four different combinations of commercially available yogurt vaccines, including starter cultures of Bifidobacterium bifidum. Both the type of vaccine and the time of storage at 8 °C had a significant effect on the folate and CLA contents in the tested fermented milks. The highest folate content (105.4 µg/kg) was found in fresh fermented milk inoculated with Lactobacillus delbrueckii, Streptococcus thermophilus, and Bifidobacterium bifidum. Only the mix of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, and Bifidobacterium bifidum showed potential (59% increase) to synthesize folate during seven days of storage. A significant increase in the content of CLA, when compared to fresh fermented milk, was observed during cold storage for up to 21 days in products enriched with Bifidobacterium bifidum.

Folate metabolic profiling and expression of folate metabolism-related genes during panicle development in foxtail millet (Setaria italica (L.) P. Beauv).

Foxtail millet grain has higher folate content than other cereal crops. However, the folate metabolite content and the expression patterns of folate metabolite-related genes are unknown. Liquid chromatography-mass spectrometry was used to investigate 12 folate metabolites in a foxtail millet panicle. The content of total folate and derivatives gradually decreased during panicle development. Polyglutamate 5-formyl-tetrahydrofolate was the major form. Twenty-eight genes involved in the folate metabolic pathway were identified through bioinformatic analysis. These genes in Setaria italica, S. viridis and Zea mays showed genomic collinearity. Phylogenetic analysis revealed that the folate-related genes were closely related among the C4 plants compared to C3 plants. The gene expressions were then studied at three panicle development stages. The gene expression patterns were classified into two groups, namely SiADCL1 and SiGGH as two key enzymes, which are responsible for folate synthesis and degradation; their expression levels were highest at the early panicle development stage, up to 179.11- and 163.88-fold, respectively. Their expression levels had a similar downward trend during panicle development and were significantly positively correlated with the concentration of total folate and folate derivatives. However, SiSHMT3 expression levels were significantly negatively correlated with total folate concentration. Besides being the major determinants of folate and folate derivatives accumulation, SiADCL1 and SiGGH expression levels are key limiting factors in the foxtail millet panicle. Therefore, SiADCL1 and SiGGH expression levels can be targeted in genetic modification studies to improve folate content in foxtail millet seeds in the future. © 2021 Society of Chemical Industry.

Folate Content of a Staple Food Increased by Fermentation of a Cereal Using Selected Folate-Producing Microorganisms

Folate deficiencies are widespread in Africa due to predominantly cereal-based diets. The objective of this work was to test the feasibility of using folate-producing microorganisms to increase folate content of tef injera, a traditional Ethiopian fermented staple food. To this end, a strain of Lactobacillus plantarum previously isolated from fermented tef batter and a commercial Saccharomyces cerevisiae were used alone and in combination to prepare injera . Ten successive fermentations using backslopping from the fermented batter prepared with L. plantarum inoculation were performed to mimic the traditional backslopping. The highest folate content was obtained with S. cerevisiae (53.5 µg/100g fresh material). All the combinations were efficient and could cover up to 22 % of the recommended nutrient intakes. All injera prepared with selected inoculums were preferred by sensory panelists to the traditional one. This work demonstrates the possibility to increase folate intake using folate-producing microorganisms in the conditions normally encountered in households.

Evolution of Folate Content During Wort Production

There are numerous scientific publications about the folate content of several types of beers available in commercial trade, but there is limited information about the effect of raw materials and technological steps of brewing on folate content. In this study the aim was to investigate different raw materials and the evolution of folate content during laboratory and pilot scale brewing. For the determination of folate content of different malts, three types of barley malts (Pilsner, Caramel, and Coloring), wheat malt, rye malt, and oat malt were analysed. For the study of the evolution of folate content during the brewing process, worts were produced on laboratory and pilot scale. Among malts, Pilsner type barley malt had the highest folate content (44.7 μg/100 g d.m.). During brewing the protein rest seemed to dissolve the majority of the folate content, and with the increasing temperature of the β- and α-amylase rests there was a further dissolution. Filtration and sparging did not have negative effect on folate concentration related to the extract content of the wort. Hop boiling had no negative effect on folate content, folate seemed to be stable during the one-hour boiling. Natural folate of barley malt seemed to be stable through the technological steps of brewing, offering the possibility to produce a foodstuff with high natural folate content.

Identification of the prepared foods promising for dietary folate intake in Beijing, China.

The aim of the present study was to analyze folate content and composition in foods consumed daily by Chinese people. The concentration of seven folate derivatives in sixty‐four selected foods was determined by a liquid‐chromatography tandem‐mass spectrometry method. The total folate levels ranged from 0.28 to 129 µg/100 g fresh weight, with an average of 21.18 μg/100 g. The highest folate content was found in boiled egg yolk and waxy corn (>120 µg/100 g), abundant folate levels in cooked vegetables such as hot pepper, spinach, soybean sprout, stem lettuce, coriander, and broccoli (44–72 µg/100 g), and lowest in Coca Cola (0.28 µg/100 g). 5‐Methyl‐tetrahydrofolate was the major folate derivative in various foods, accounting for 72% of the total folates on average, with the highest being 90% in egg yolk. These data will enable estimation of the daily folate intake and allow dietary recommendations to improve folate status in humans.

Effect of Different Cooking Methods on Folate Content in Chicken Liver.

Common liver sources in European countries include cow, chicken, duck, lamb and pig. Despite its decreasing popularity, liver is possibly one of the most nutrient-dense foods, being rich in high-quality protein and low in calories. In animals, the liver is the storage organ for folate. In this study, the effect of different cooking methods on folate vitamers content in chicken liver was investigated. Three folate derivatives, 5-CH3-H4PteGlu, H4PteGlu and 5-HCO-H4PteGlu, were identified in the analyzed samples using high performance liquid chromatography (HPLC). The folate content in liver after sous-vide (60 °C/75 min) and steaming (100 °C/30 min) did not differ significantly (p ≤ 0.05) from raw liver folate content (781 µg/100 g). Even liver cooked in a combi oven or grilled (which resulted in significant folate losses) showed much higher folate content, 455–631 µg/100 g and 612–715 µg/100 g, respectively, than the most folate-abundant plant foods. These findings are important as they demonstrate that processed liver has the potential to improve the supply of folate and meet the recommended daily requirements, particularly when folate deficiency is common worldwide.

Microalgae a Superior Source of Folates: Quantification of Folates in Halophile Microalgae by Stable Isotope Dilution Assay.

A multitude of human nutritional supplements based on Chlorella vulgaris biomass has recently been introduced to the specialty food market. In this study, an analysis of total folate contents in Chlorella sp. and a series of marine microalgae was conducted to evaluate folate content in alternative algae-based food production strains. For the first time, total folate content and vitamer distribution in microalgae were analyzed by stable isotope dilution assay (SIDA) using LC-MS/MS, which has demonstrated its superiority with respect to folate quantification. Consistently, high folate contents were detected in all examined microalgae samples. High folate concentrations of 3,460 ± 134 μg/100 g dry biomass were detected in freshly cultivated Chlorella vulgaris, notably also in other well-researched microalgae strains. To that end, the highest folate content currently documented for any algae sample was measured in the marine microalgae Picochlorum sp. isolate with values of 6,470 ± 167 μg/100 g dry biomass. This calls for alternative products based on other algae biomass. Our data indicate that freshwater and marine microalgae provide extremely high concentrations of folates, which warrant further studies on the regulation of pteroylpolyglutamates in algae as well as on bioaccessibility, absorption, and retention in humans.

Single Nucleotide Polymorphism (SNP) markers associated with high folate content in wild potato species.

Micronutrient deficiency, also known as the hidden hunger, affects over two billion people worldwide. Potato is the third most consumed food crops in the world, and is therefore a fundamental element of food security for millions of people. Increasing the amount of micronutrients in food crop could help alleviate worldwide micronutrient malnutrition. In the present study, we report on the identification of single nucleotide polymorphism (SNP) markers associated with folate, an essential micronutrient in the human diet. A high folate diploid clone Fol 1.6 from the wild potato relative Solanum boliviense (PI 597736) was crossed with a low/medium folate diploid S. tuberosum clone USW4self#3. The resulting F1 progeny was intermated to generate an F2 population, and tubers from 94 F2 individuals were harvested for folate analysis and SNP genotyping using a SolCap 12K Potato SNP array. Folate content in the progeny ranged from 304 to 2,952 ng g-1 dry weight. 6,759 high quality SNPs containing 4,174 (62%) polymorphic and 2,585 (38%) monomorphic SNPs were used to investigate marker-trait association. Association analysis was performed using two different approaches: survey SNP-trait association (SSTA) and SNP-trait association (STA). A total of 497 significant SNPs were identified, 489 by SSTA analysis and 43 by STA analysis. Markers identified by SSTA were located on all twelve chromosomes while those identified by STA were confined to chromosomes 2, 4, and 6. Eighteen of the significant SNPs were located within or in close proximity to folate metabolism-related genes. Forty two SNPs were identical between SSTA and STA analyses. These SNPs have potential to be used in marker-assisted selection for breeding high folate potato varieties.

Total folate content in ready-to-eat vegetable meals from the Spanish market

Currently, data concerning the amount of naturally occurring dietary folate in ready-to-eat foods is scarce, in spite of an increasing consumption. Total folate (TF) in 35 chilled ready-to-eat vegetable-based meals, expected to be good natural folate sources, was determined. A validated method that relies on a trienzyme extraction (α-amylase, rat plasma conjugase as a substitute to chicken pancreas conjugase, and protease enzymes), and chloramphenicol-resistant Lactobacillus casei subspecies rhamnosus folate dependent growth was developed. No previous reports on folate data in refrigerated ready-to-eat foods from the Spanish market have been published. Vegetable burgers, containing pulses and green leafy vegetables, had the highest folate content (103.8μg TF/100g fresh weight). Refrigerated recipes including chickpeas, peas, broccoli, or artichokes had an average folate content of 75μg TF/100g fresh weight. A typical serving of targeted ready-to-eat meals (175g) may provide up to 60% of the Dietary Reference Intake for an adult. No differences in TF were found after mild heat treatment, as recommended by the manufacturer before its consumption. Therefore, these products may be used to increase folate intake by choosing naturally rich dietary sources. Data will assist dietary studies in the assessment of population folate dietary intakes.

Distribution of bioactive compounds in maize fractions obtained in two different types of large scale milling processes

Maize kernels contain different bioactive compounds that are important for human health. The aim of this study was to analyze the distribution of the bioactive compounds in maize fractions derived from two industrial dry-milling processes, characterized by a dry-degermination (DD) system and a tempering-degermination (TD) system.The bioactive compounds in maize resulted unevenly distributed in the milling fractions of the kernel. By-products such as the germ and the animal feed flour, had higher total antioxidant capacity (TAC), total polyphenol content (TPC) and total dietary fibre content (TDF) than the whole grains, while xanthophyll and resistant starch resulted to be higher in the fractions derived from the vitreous endosperm. The germ fraction showed also the highest folate content. Results also showed that the type of degermination process influences the bioactive compound contents in the milling fraction, in accordance to the effectiveness of the germ and bran removal from the endosperm fractions. In particular, the animal feed flour obtained by means of TD system resulted in a higher TAC, TPC and TDF than the same fraction obtained by means of the DD system. Conversely, the extraction rate do not affect the recovery of bioactive components in particular fractions.