Highest DPPH Research Articles

Nutritional value, phytochemical content, and pharmacological screening of Borassus flabellifer L. fruits.

The present study aimed to evaluate the nutrition value, phytochemical content, and diverse pharmacological activities of different solvent extracts of Borassus flabellifer L. fruit. Among all, the hydro-alcoholic extract showed high DPPH and ABTS radical scavenging activities with IC50 values of 82.98 ± 0.93, and 75.67 ± 0.48 μg/mL, respectively. The hydro-alcoholic extract offered a noticeableα-amylase (IC50 = 82.90 ± 0.73 µg/mL) and α-glucosidase inhibitory effects (IC50 = 62.5 ± 0.39 µg/mL), which were comparable to standard drug Acarbose. In anti-denaturation assay, the hydro-alcoholic extract showed a weaker inhibitory effect (IC50 = 93.47 ± 0.93) than standard Diclofenac-Na (IC50 =65.85 ± 0.42 µg/mL). Furthermore, the hydro-alcoholic extract showed the highest potential in preventing the harmful effects of UV radiation with a sun protection factor of 37.12 ± 0.28.

Nutritional and Bioactive Compounds, Antioxidant Properties and Antibacterial Activity of Chinese Chive (Allium odorum L.).

The present study was conducted to generate the comprehensive data on the nutritional composition, minerals, heavy metal, bioactive compounds, antioxidant and antibacterial properties of Chinese chive (Allium odorum L.) grown at two different locations including Manipur and Punjab. The crop grown in Manipur exhibited higher values of nutritional and phytochemical compound values than those grown in Punjab. It exhibited significantly higher amounts of crude protein, crude fiber, potassium, calcium and iron content in the Manipur environment. The allicin content in leaves of Chinese chive on a dry weight (dw) varied from 1.31 to 1.4% and there was non-significant difference in allicin content at both locations. Besides, the heavy metal content of Chinese chive was much below the permissible limits of the World Health Organization. The IC50 value of Chinese chive was 78.908-95.140µg/ml which imparts strong antioxidant activity and higher DPPH (2,2-diphenyl-1-picrylhydrazyl)radical scavenging activity higher in fresh leaves than dried leaves. Total phenol and total flavonoid were found to be significantly high. In addition, high antibacterial activities were observed against toxin producing pathogenic bacterial strains of Staphylococcus aureus and Enterococcus faecalis. The nutritional and bioactive compounds, antioxidant properties and antibacterial activity of Chinese chive make it ideal for its use as a functional food and/or supplement.

Antioxidant Potential and Characterization of Polyphenol Compounds in Moringa oleifera Pods.

The aim of this investigation was to comparatively assess the antioxidant and polyphenol compounds in fresh moringa pods sourced from two different regions in Australia, namely Queensland (QLD) and Western Australia (WAU). Total polyphenol content varied between 1.64 and 5.97 mg GAE/g in moringa pod samples from QLD, while it ranged from 2.84 to 4.31 mg GAE/g in WAU samples. Total flavonoid content in QLD and WAU samples averaged 4.62 and 4.24 mg QE/g, respectively. Total condensed tannin content in QLD and WAU samples averaged 2.07 and 1.60 mg CE/g, respectively. The QLD samples had higher DPPH (2.87 vs. 2.74 mg AAE/g), ABTS (15.0 vs. 12.9 mg AAE/g), and total antioxidant capacity (2.34 vs. 1.46 mg AAE/g) than WAU samples. LC-ESI-QTOF-MS/MS analysis identified 111 polyphenol compounds in moringa pod samples, including phenolic acids, flavonoids, and tannins. Some compounds were prevalent across most samples, such as 3-sinapoylquinic acid and theaflavin. The study revealed that moringa pods contain a high concentration of polyphenols with strong antioxidant capacity. These findings highlight the substantial influence of regional effects on the polyphenol content and bioactive properties of moringa pods.

Stage of lactation, parity, breed, milk composition and minerals affect the non-enzymatic antioxidant activity of sheep milk

The aims of this study were to i) characterize sheep milk for non-enzymatic antioxidant activity via 2 different assays, namely the ferric reducing antioxidant power (FRAP) and the 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), and ii) investigate the effect of milk composition and animal-related parameters on these 2 assays by using Generalized Additive Mixed Model (GAMM) approach. A total of 740 ewes belonging to Massese and Comisana breeds were sampled once during the morning milking across 11 sampling sessions. All milk samples were analyzed for fat, protein, casein, and lactose, somatic cell score (SCS) and minerals (Ca, Mg, Na and Cl). The FRAP and DPPH assays were tested to measure the non-enzymatic antioxidant activity of milk, expressed as μM eq. ascorbic acid/mL of milk and % of inhibition, respectively. The GAMM model included the effect of parity and breed as parametric terms, and the effect of days in milk (DIM), milk yield and the interactions protein × fat, casein × SCS, Ca × Mg and Na × Cl as smooth terms. The sampling day was included in the model as random effect. Results revealed that the non-enzymatic antioxidant capacity of sheep milk, expressed as FRAP, was affected by DIM, potentially because of changes in milk composition over time. Conversely, parity and breed of ewes affected DPPH, suggesting that age- and breed-specific factors are related to specific components in milk acting as hydrogen donors. Milk fat and high casein percentages were found to significantly affect FRAP, while protein content was crucial for high DPPH levels. Additionally, Ca and Mg emerged as important non-enzymatic antioxidants for both FRAP and DPPH, highlighting their important role in antioxidant activity of sheep milk. On the other side, combinations of Na and Cl were particularly influential for FRAP, revealing the complex relationship between these minerals and non-enzymatic antioxidant activity of milk. These findings offer valuable insights into the factors affecting the antioxidative properties of sheep milk, highlighting the need for further exploration of other non-enzymatic antioxidants and their contribution to the total antioxidant activity.

메밀전초와 다시마추출물을 활용한 효소식품의 개발

The polyphenol content of common buckwheat, Tartary buckwheat, buckwheat leaves, sea tangle, sea mustard ears, and fusiformis were analyzed. Among these, buckwheat leaves exhibited the highest polyphenol content. Hot water extraction followed by treatment with plantase enzyme increased the polyphenol yield by 56% for buckwheat leaves and by 34% for sea tangle. Cytotoxicity test performed on 3T3-L1 pre-adipocytes for seaweeds and buckwheat leaves revealed no significant cytotoxic effects compared to that of the control group at 1 mg/mL. Additionally, when examining the effects of buckwheat leaf and sea tangle extracts on the pre-adipocytes differentiation, into adipocytes confirmed that buckwheat leaf extract inhibited fat differentiation at 10 mg/mL and sea tangle at 0.1 mg/mL. Buckwheat/sea tangle enzyme food had 6.5 times higher amylase activity, 27 times higher protease activity, and more than twice as high dietary fiber, polyphenol, and DPPH scavenging ability as brown rice enzyme foods. Buckwheat/sea tangle enzyme-supplemented food contained more than 30% more phlorotannins and 7.8% more alginic acid than brown rice enzyme-supplemented food.

Comparative assessment of antioxidant and antiglycation properties of Leucas species

Abstract BACKGROUND: The genus Leucas is an aromatic plant and belongs to the Lamiaceae family. They are rich in secondary metabolites. The benefits of Leucas in curing many ailments such as coughs, colds, diarrhea, diabetes, inflammation, and skin diseases have made it an Ayurvedic component. Hence, the present study aims to evaluate the phytochemical, antioxidant, and antiglycation properties of methanol and aqueous extract from three Leucas species, viz, Leucas aspera Link, Leucas zeylanica (L.) W.T.Aiton, and Leucas longifolia Benth. METHODS: Dry powders of the whole part of three species of Leucas were extracted with methanol and aqueous solution. Qualitative analysis of secondary metabolites such as flavonoids, phenolics, alkaloids, glycosides, terpenoids, and tannins was performed by using standard phytochemical procedures. Total phenol, flavonoid, and alkaloid contents were estimated by spectrophotometric methods. The superoxide radical scavenging activity, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, hydrogen peroxide radical scavenging activity, and ferric-reducing power methods are in vitro models. Bovine serum albumin glycation assay was used to evaluate the in vitro antiglycation activity of extracts. RESULTS: The highest percentage of extraction yield was observed in the methanolic extract of all Leucas species compared to the aqueous extract. The preliminary phytochemical tests reveal the presence of various bioactive secondary metabolites such as phenols, flavonoids, alkaloids, steroids, terpenoids, and glycosides. These extracts show that L. aspera has high DPPH activity, superoxide radical scavenging activity, hydrogen peroxide radical scavenging activity, and ferric-reducing antioxidant power compared to L. zeylanica and L. longifolia. The methanolic and aqueous extract of L. aspera exhibited more significant inhibitory (96.70 ± 1.78 and 90.33 ± 1.65%) effects on advanced glycation end products formation in bovine serum albumin glycation systems compared to standard aminoguanidine (99.45 ± 1.37%) than L. zeylanica and L. longifolia. The methanol extract of L. aspera is rich in phenols, flavonoids, and alkaloids and has strong antiglycation and antioxidant properties. CONCLUSION: The strong antiglycation and antioxidant activity of all three Leucas species suggests its possible role in combating diseases associated with diabetic complications and oxidative stress.

The preparation of fish scale gelatin-polydopamine photothermal sterilization film and its application for storage of cherry tomato

In this study, polydopamine nanoparticles (PDA NPs) were synthesized and fish scale gelatin (FSG) was extracted by hot water hydrolysis. The film for photothermal sterilization was created by casting FSG with the addition of PDA NPs. The results indicated that the PDA NPs were able to heat up to 46 °C in 5 min under near-infrared (NIR), demonstrating good photothermal conversion. The PDA NPs/FSG solution showed short-term bactericidal activity against Staphylococcus aureus and Escherichia coli, with a bactericidal rate of more than 99 %. The PDA NPs/FSG film could warm up to 42.6 °C under NIR irradiation for 5 min. With the addition of 0.3 % of PDA NPs, the PDA NPs/FSG films showed high UV absorption, DPPH and ABTS radical scavenging ability, and high water contact angle (WCA), improving the barrier to water and oxygen and the tensile strength. The 0.3 % PDA NPs/FSG film triggered by NIR effectively maintained the firmness, soluble solids content and appearance, and delayed the weight loss of cherry tomatoes. Thus, the PDA NPs/FSG films had good photothermal antimicrobial activity and could be used for extending the shelf life of cherry tomatoes.

The ANTIOXIDANT AND PHYTOCHEMICAL ANALYSIS OF METHANOL EXTRACT OF PHYLLANTHUS AMARUS

Phyllanthus amarus is a widely recognized plant that has been traditionally utilized to cure a variety of ailments. The current study explored the phytochemical and antioxidant characteristics of P. amarus methanol extract. Standard procedures were used to analyze: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) percentage (%) inhibition, lipid peroxidation % inhibition, total phenolic content (TPC) and total flavonoid content (TFC). A higher quantity of the plant extract resulted in a greater inhibition of DPPH and lipid peroxidation percentage. The highest DPPH % inhibition (74.4%) and lipid peroxidation % inhibition (83.44%) were at 0.05 mg/ml while the lowest DPPH % inhibition (25.23%) and lipid peroxidation % inhibition (39.11%) was at 0.03 mg/ml (p < 0.05). The most prevalent compound was flavonoid, with a TFC of 460.22 µg/ml while the TPC was 7.428 µg/ml. Owing to its antioxidant and phytochemical qualities, P. amarus methanol extract may potentially find application in medicine.

Bioprospecting of novel and biologically active compounds and enzymes from a new fungal biobank for industrial biotechnology

Here, we present methods for screening any newly isolated microbial biobank for enzymes, antioxidants, and additional secondary metabolites. Bioactive compounds and enzymes produced by fungi have many applications in industry as valuable leads for pharmaceuticals, nutraceuticals, and potential strains for industrial biotechnology. In this study, fungi collected from Irish habitats were purified and identified by sequencing Internal Transcribed Spacer (ITS) ribosomal DNA regions. Reducing sugars released because of enzyme hydrolysis was used to estimate the relative enzyme activity for carboxymethyl cellulase, pectinase, β-xylanase, arabinoxylanase, mannanase, and galacto-mannanase in the fungal extracts and was normalized against the crude protein content to express relative enzyme activity/milligram. The best enzyme producers were as follows: F1 Clonostachys rosea demonstrated 351.63 U/mg β-xylanase and 645.50 U/mg arabinoxylanase activity; F2 Penicillium expansum had 25 U/mg cellulase and 112.5 U/mg pectinase activity; F3 Fusarium avenaceum had 139.36 U/mg arabinoxylanase, 52.33 U/mg galacto-mannanase, and 45 U/mg mannanase activity; and F9 Trichoderma koningii had 372.15 U/mg β-xylanase and 655.18 U/mg arabinoxylanase activity. In addition to enzyme activity, these strains also exhibited antioxidant activity when tested in Oxygen radical absorbance capacity (ORAC), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric ion reducing antioxidant power (FRAP) assays, with activity expressed as μmol Trolox equivalents (TE) per gram of dried fungal extract. In results from the ORAC assay, F1 C. rosea reported 655,669 µmol TE/g; F2 P. expansum had 636,889 µmol TE/g; and F3 F. avenaceum had 4,488,035 µmol TE/g. High DPPH activity was observed for F1 C. rosea at 12,572 µmol TE/g, F3 F. avenaceum at 22,646 µmol TE/g, and F9 T. koningii at 22,558 µmol TE/g. F1 C. rosea, F3: F. avenaceum, and F9 T. koningii also reported high FRAP activity at 36,019 µmol TE/g, 69,325 µmol TE/g, and 25,812 µmol TE/g, respectively. In terms of secondary metabolites, the main compounds detected for the isolates were F1 C. rosea produced benzoic acid, 3-pyridinepropionic acid, and cyclo(-Gly-Phe); F2 P. expansum produced Cyclo(-Gly-Phe) and prenitremone A; F3 F. avenaceum produced cyclo(-Leu-Leu) and p-anisaldehyde; and F9 T. koningii produced cyclo(-Gly-Phe), canescin, citreoviridin X, and cyclopaldic acid. These selected isolates indicate that Irish fungi are a potential source of enzymes and novel biologically active compounds useful in industrial biotechnology and bioproduction supporting sustainability and responsible environmental management.

Phytochemical Profiles and Biological Activities of Five Wild Camellia Species from Ta Dung, Vietnam.

The C. luuana Orel & Curry (TD3), C. furfuracea (Merr.) Cohen-Stuard (TD4), C. bidoupensis Truong, Luong & Tran (TD6), C. sinensis (L.) Kuntze (TD7), and C. kissii var. spp (TD8), have been traditionally used as a health-promoting beverage by local people in Ta Dung, Dak Nong. Despite their potential health benefits, further scientific data on biological and phytochemical properties of these plants is needed. To address this issue, this study was conducted to investigate phytochemical and biological properties of five Camellia species extracts, using DPPH, ABTS radical scavenging, copper chelating (Cu-chelator), and tyrosinase inhibition (TI), α-amylase (Al-AI), and α-glucosidase (Al-GI) analyses. As results, ten compounds were identified using UPLC method, in which catechins (mainly EGCG and catechin (Cat)), were the most prevalent, and followed by chlorogenic acid (ChlA), quercitrin (Querci), rutin, and quercetin (Querce). Additionally, multiple factor analysis (MFA) also revealed that TD7, TD3, and TD4 containing high TPC, TFC, high concentrations of EGCG, ChlA, and caffeine were responsible for their high DPPH, ABTS radical scavenging activities, as well asTI, Al-AI and Al-GI. Furthemore, TD6 and TD8, possessing elevated levels of Apig, Querci, Rutin, Querce, Cat, and EA, exhibited a high Cu-chelator property, but a weak enzyme inhibition. From all above-mentioned results, the antioxidative and enzyme inhibitory potentials of Camellia species extracts collected in Dak Nong province in Vietnam were scientifically demonstrated paving a pathway to develop health supplement in further studies.

Phytochemical Analysis, Cytotoxic, Antioxidant, and Anti-coccidian Activities of Vitis vinifera Leaves Grown in Saudi Arabia

Background: Natural products are essential for medicinal purposes in developing countries. Traditional and contemporary medicine utilized plant products as alternative treatments for parasitic diseases. Objectives: The purpose of the investigation is to analyze and assess the bioactive compounds in Vitis vinifera leaf extracts, as well as their effect on coccidiosis. Materials and methods: Several analytical techniques were utilized, such as infrared spectroscopy (IR) and gas chromatography-mass spectrometry (GC-MS). Additionally, we assessed the total phenol, tannin, flavonoid examination, and cytotoxicity of the plant extracts using an MTT assay and UV-visible spectrophotometry (UV-Vis). Moreover, the samples’ levels of antioxidant activity (measured with DPPH and ABTS) were analyzed. Results: The leaf extract's GC-MS study identified many bioactive elements linked to plant secondary metabolites. Further, the total phenol, tannin, and flavonoid were 160.217 ± 0.652, 65.872 ± 0.263, and 34.0463 ± 0.545 (mg TAE/g DW), respectively. It also contains these extracts with a high antioxidant and DPPH radical scavenging activity. This reflects the number of bioactive chemicals that are present in these extracts. Conclusions: The findings of this research discuss techniques in phytochemical analysis of Vitis vinifera leaf extracts and the identification of biologically active components that support and justify the utilization of grape leaves in folk medicine in developing countries.

Green synthesis of platinum nanoparticles using aqueous bark extract of Quercus sp. for potential antioxidant and antimicrobial applications

Oak bark, which is commonly used in the wood industry, has by-products often repurposed as fuel. Its extracts are rich in compounds with anticancer, antibacterial, antifungal, and anti-inflammatory properties. This study synthesized platinum nanoparticles (PtNPs) using aqueous extracts from Quercus dalechampii (QD), Q. frainetto (QF), and Q. petraea (QP). Key factors during nanoparticle formation included reaction time, metal ion concentration, pH, extract-to-metal ion ratio, and temperature. The PtNPs were characterized by dynamic light scattering, Fourier transform infrared spectroscopy, and transmission electron microscopy. The average diameters were 58.5 ± 7.6 nm for QD-PtNPs, 41.6 ± 5.4 nm for QF-PtNPs, and 41 ± 5.3 nm for QP-PtNPs. Antioxidant and antimicrobial activities were also analyzed. The QP-PtNPs had the highest DPPH (2,2-Diphenyl-1-picrylhydrazyl), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (Cupric Reducing Antioxidant Capacity) free radical scavenging activities, while QD-PtNPs excelled in ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) scavenging. All PtNPs showed strong antimicrobial properties, particularly against Enterococcus faecalis, Escherichia coli, Candida krusei, and Candida auris. These findings suggest that Quercus-mediated PtNPs have significant potential for developing treatments against bacterial and fungal infections, with promising applications in medicine.

Antioxidant Potential and Phytochemical Profile of Althaea (Hatmi) and Hibiscus Flower Extracts: A Comprehensive Analysis

In this study, the bioactive components and antioxidant properties of Althaea (Hatmi) and Hibiscus plants were assessed using various methods. Both aqueous and ethanol extracts of these plants yielded distinct and effective results. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Ferric Reducing Antioxidant Power (FRAP) assays at concentrations of 25, 50, 75, and 100 mg/mL. Hatmi extracts, both ethanol and aqueous, exhibited high DPPH activity, particularly at 75 and 100 mg/mL, while Hibiscus showed a linear increase in DPPH activity with concentration, reaching 2000 µM Trolox Equivalent (TE) /g dry weight (DW) at 100 mg/mL. In ABTS assays, lower concentrations of ethanol extracts were more effective, but higher aqueous concentrations showed greater activity. FRAP results indicated high antioxidant activity in Hatmi ethanol extracts, with activity reaching 2700 µM TE/g DW at higher concentrations. Phenolic analysis revealed high levels of apigenin 7-glucoside, hesperidin, and caffeic acid in Hatmi, while Hibiscus extracts contained significant amounts of chlorogenic acid and quercetin. Gas Chromatography-Mass Spectrometry (GC-MS) analysis showed that Hatmi had a higher abundance of volatile organic compounds compared to Hibiscus.

Phytochemical profiles, bioactivities, and molecular docking and molecular dynamics approaches of endemic Campanula baskilensis Behçet (campanulaceae)

Campanula species have phenolic derivatives with a broad biological potential. This study aimed to investigate the chemical contents, bioactivities, and prediction of the biological effect mechanism using the molecular docking study for C. baskilensis (CB) leaf (L), root (R), and stem (S) extracts.CBL gave the highest phenolic contents as hesperidin at 7.56 mg/g extract. Total phenolic and flavonoid contents of CBS were determined as 3.12 ± 0.05 mg GAE/g and 1.11 ± 0.11 mg QE/g, respectively. CBS and CBL exhibited high DPPH• scavenging, H2O2 scavenging, AChE, BChE, carbonic anhydrase, lipase, and tyrosinase inhibition effects and demonstrated potent antimicrobial activity. The strong phenolic components of CBS and CBL extracts contributed to their biological activities. According to the molecular docking results, a high inhibition effect was observed on the inhibition activities due to the presence of hesperidin, which is the highest main component of the extracts. Additionally, docking studies were performed on hesperidin for the first time. Dynamics study showed that the interaction between the hesperidin and BChE increased stability, producing a stable complex form within 100 ns. In conclusion, C. baskilensis extracts, especially hesperidin, may be promising potential sources for neurological diseases and complementary medicine.

Enhancing physicochemical properties, organic acids, antioxidant capacity, amino acids and volatile compounds for ‘Summer Black’ grape juice by lactic acid bacteria fermentation

To investigate the effect of lactic acid bacteria fermentation on the quality of ‘Summer Black’ grape juice, this study characterized the differences in physicochemical properties, organic acids, antioxidant capacity, amino acids and volatile compounds among different treatments. The results showed that the pH and total sugar of all fermentation groups were lower than those of the CK (control group) sample. ZR (Lactobacillus plantarum) sample had the highest DPPH and OH− scavenging ability, and the total phenol and total flavonoid content of SR (Streptococcus thermophilus) sample were increased by 5.2% and 4.1%, respectively. The contents of tartaric acid, malic acid, and citric acid were improved significantly by LAB fermentation. The content of Asp, Thr, Ser, Glu, and Gly in the SR sample was significantly higher than in the CK sample. Phenylethyl alcohol, lactic ethyl ester, L-menthol, citronellol, and nerol were produced by LAB fermentation, giving fruit and floral aroma, and streptococcus thermophilus has a higher potential to enhance the aromatic flavor of ‘Summer Black’ grape juice. 20 chemical indicators were determined to explain the differences between groups by OPLS-DA.

Influence of white radish (Raphanus sativus L.) powder on the quality characteristics of set-type yogurt during cold storage

ABSTRACT The quality attributes of a set-type yogurt fortified with different levels (0%, 2%, and 4%) of white radish powder (WRP) during cold storage were evaluated. WRP contains high total phenolic content (TPC, 276.8 mg GAE/100 g) and fiber (11.8%) and possessed high DPPH scavenging activity (63.35% inhibition). WRP increased the ash, fiber, TPC, DPPH scavenging activity, viscosity, acidity, water holding capacity (WHC), a*, b*, cohesiveness, springiness, and storage modulus (G´) and reduced the pH, total solids, syneresis, hardness, adhesiveness, protein, fat, L*, thiobarbituric acid (TBARS), gumminess, color, flavor, taste, smoothness, sourness, and general acceptability of yogurt. The pH, viscosity, WHC, hardness, gumminess, adhesiveness, TPC, DPPH scavenging activity, and sensory characteristics were reduced, whereas syneresis, acidity, a*, b*, G´, TBARS were augmented in all yogurt during storage with WRP-yogurt showed less changes indicating high storage stability of WRP-yogurt. Conclusively, WRP improved the quality attributes and storage stability of a set-type yogurt.

Antioxidative properties, phenolic compounds, and invitro protective efficacy of multi-herbal hydro-alcoholic extracts of ginger, turmeric, and thyme against the toxicity of aflatoxin B1 on mouse macrophage RAW264.7 cell line.

Aflatoxin B1 (AFB1), the most potent toxic and carcinogenic secondary fungal metabolite, has frequently been reported in food/feed. Nowadays, herbal extracts are considered safe dietary additives to reduce the toxicity of such compounds. The protective capability of various combinations of hydro-alcoholic extracts (HAEs) of ginger, turmeric, and Shirazi thyme, against the toxicity of AFB1 on the RAW264.7 cell line was investigated. The RAW264.7 cells were exposed to six different concentrations of AFB1 (0.09, 0.18, 0.37, 0.75, 1.5, and 3 μg mL-1) for 48 h to determine the IC50 of AFB1. AFB1 was estimated to have an IC50 of 1.5 μg mL-1 for RAW264.7 cells. Then, the cells were simultaneously incubated with 1.5 μg mL-1 AFB1 and the HAEs for 24 h. The HAEs significantly reduced the toxicity of AFB1 in RAW264.7 cells. HAE of Shirazi thyme showed the highest amount of total phenol content (TPC) and the highest DPPH• activity. In addition, a combination of ginger, turmeric, and Shirazi thyme extract showed the highest antioxidant activity. Rutin, quercetin, and apigenin were the main phenolic components of ginger HAE. A significantly positive correlation was observed between TPC of hydro-alcoholic extract with ferric reducing antioxidant power (FRAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) values. Consequently, the simultaneous consumption of such extracts is recommended to protect the cells against dietary toxins.

Anthelmintic activity of crude and separated extract of Aloe vera (Xanthorrhoeaceae) against bovine adults parasites of Onchocerca ochengi and infected larvae of drug resistant strains of the free-living nematode Caenorhabditis elegans.

Human onchocerciasis is caused by the filarial worm. Onchocerca volvulus is a parasite that forms nodules under the skin. The aim of this work was to assess the nematicidal activities of Aloe vera on Onchocerca ochengi and Caenorahbditis elegans and to determine the phytochemical compounds. Nodules were collected from the umbilical region of infected cattle, dissected and male worms were cultured in RPMI-1640. Worms were incubated with different concentrations of A. vera extracts in RPMI-1640 and M9-buffer. Polyphenol, tannin and flavonoid contents of extract were determined by using gallic acid and rutin as standards. The anthelmintic effect of A. vera extract against O. ochengi was concentration dependent with LC50 of 20.71µg/mL and 11.75µg/mL after 48 and 72h respectively. A. vera extract exerted concentration dependent lethal effects (LC50 = 2747 and LC50 = 31,937µg/mL) against C. elegans (Wild Type). Methanolic-methylene chloride (MeOH-CH2Cl2) of A. vera extract exhibited high DPPH activity with an IC50 value of 15µg/mL and 9µg/mL for ascorbic acid. The highest activity in adult worms was observed with the MeOH (100: 0) and AcOEtMeOH fractions with LC50 values of 12.82 and 15.50µg/mL respectively. EcOEtMeOH (8:2 v/v) was more effective (LC50 = 250µg/mL) on WT of C. elegans. A. vera contains polyphenols (1015.05 and AcOEtMeOH = 893.60), flavonoids (25.35 and MeOH = 225.76) and tannins (401.37 and Hex = 788.89). A. vera showed in vitro nematicidal activity against O. ochengi and C. elegans. A. vera could be used as an alternative anthelmintic for onchocerciasis treatment.

Effect of foaming conditions and drying temperatures on total polyphenol content and drying rate of foam-mat dried banana powder: Modeling and optimization study

This study aims to optimize the parameters of the foam-mat drying to produce banana powder. Input parameters of the foam drying such as foaming agent (egg albumin) concentration used from 5 to 15 %, the foam stabilizer (maltodextrin) used from 1 to 3 % and the drying temperature varied between 60 and 80 °C. Factors with 3 levels are arranged according to the Box-Behnken design, which further modelled by RSM (response surface methodology) and ANN (artificial neural network), and optimized. The drying rate and total polyphenol content (TPC) of banana powder under the studied conditions were determined as the target output. The moisture, color, total polyphenol, antioxidant activity and some physical parameters of final fine powder were analyzed. Increasing temperature has increased the drying rate. In addition, increasing the concentrations of egg albumin and maltodextrin maintained the highest TPC and maximum drying rate. ANN model showed the higher forecasting capacity than that of RSM. Moreover, simultaneous optimization of two responses (TPC and drying rate) was selected to maximize the desired value at the concentration of albumin, maltodextrin and drying temperature of 11.86 %, 1.92 %, 74.94 °C, respectively, corresponding to the highest TPC value and drying rate of 1.31 mgGAE/g DW and 2.48 g water/g dry matter/min. At this condition, the drying time was recorded as 103 min. Validation of the optimal ratios showed that the experimental values of TPC and drying rate were in good agreement with the model predicted data. The moisture content and water activity of product were found to be 5.87 ± 0.07 % and 0.37 ± 0.01. The product had bright colors with L*, a* and b* values were measured as 86.4 ± 0.5, 1.75 ± 0.08 and 15.2 ± 0.3, respectively. The high DPPH radical scavenging activity was detected (53.5 %) with the water solubility index and water absorption index of banana powder was determined at value of 56.98 % and 5.17 g/g, respectively. Foam mat dried banana powder was well preserved in paper packaging with aluminum foil.

Chitosan‒gellan gum polyelectrolyte hydrogel beads containing tea tree oil microcapsules: Preparation, characterization and application

Encapsulation can be applied for essential oil protection, increasing the probability of its application in food preservation. In this study, tea tree oil (TTO) microcapsule powder (MP) was prepared by a spray‒drying approach with soy protein isolate (SPI) and fructooligosaccharide (FOS) as wall materials and were subsequently further encapsulated by chitosan (CH) and gellan gum (GG) via a polyelectrolyte complex coacervation approach. The hydrogel beads prepared with a GG mass ratio of 25% presented the best appearance and the densest internal structure. FTIR and Raman spectral analyses demonstrated the generation of hydrogen bonds between CH and GG. Thermogravimetric analysis confirmed that complex coacervates formed by CH and GG as wall materials can improve the thermal stability of TTO. Owing to the excellent barrier properties of the hydrogel beads, the hydrogel beads maintained high DPPH and ABTS free radical scavenging activities after storage. Moreover, the sustained release of TTO hydrogel beads has potential for preserving fresh-cut banana. These discoveries indicate that hydrogel beads can be used as ideal carriers of essential oils such as TTO and may be used in food preservation in the future.