High nitrogen (N) efficiency crop genotypes utilize resources rationally and reduce N losses. To evaluate the extent of genotypic variation in response to N supply, twenty-four maize genotypes were grown in Soilrite (peat mass, vermiculite and pearlite mix) supplied with low N level (2mM) or normal N level (25mM) in Hoagland solution. At 21 DAS the average shoot height (SH), leaf area (LA), stem dry weight (SDW), leaf dry weight (LDW), above ground dry weight (ADW), root dry weight (RDW) and total plant dry weight (PDW) were 38.1, 257.5, 67.3, 121.5, 96.9, 49.4, 76.9 and 140.6% higher respectively in seedlings exposed to normal N treatment than those grown under low N level. The shoot N content (SNC), root N content (RNC), above ground N accumulation (ANA), root N (RNA) and total plant N accumulation (PNA) were found to be 1.79, 2.23, 3.51, 3.30 and 3.44-fold increased at normal N supply over low N. Coefficients of variation (CV) varied greatly and ranged from 10.9-37.8 and 9.4-36.7 under low-N and normal-N treatment, respectively. LA, SDW, LDW, ADW, TDW, SNC, RNC, ANA, RNA and TNA showed significant positive correlation both under low N and normal N regime (p< 0.05). SDW, LDW, ADW, PDW, SNC, RNC, ANA, RNA and PNA were significantly correlated and had high CVs reflecting the difference of N in different maize genotypes and these parameters were selected for calculation of N efficiency comprehensive index. According to the N efficiency comprehensive index scatter map under low and normal N conditions, genotypes HKI-164-D4, HKI-325-17 AN, HKI-1332, DTL-3, DTL-2, DHM-117 and DTL-1 were efficient under both low and normal-N conditions and genotypes DTL-10, SNJ-2011-96, PSRJ-13099, NSJ-211 and Z59-17were inefficient under both low and normal-N conditions