High Carbohydrate Research Articles

Role of AIM2 and cGAS-STING signaling in high fat high carbohydrate diet-induced gut dysbiosis associated neurodegeneration.

Gut dysbiosis modulates CNS complications and cognitive decline through the gut-brain axis. The study aims to investigate the molecular mechanisms involved in gut dysbiosis-associated cognitive changes and the potential effects of probiotics in high fat-high carbohydrate diet-induced gut dysbiosis-associated neurodegeneration. We used high fat, high-carbohydrate diet (HFHCD) and high-fat diet (HFD) to induce gut dysbiosis-associated neurodegeneration in C57BL/6 mice. IVIS imaging system and biochemical changes using ELISA measured intestinal inflammation. We used fecal samples for qPCR profiling of intestinal bacteria, and serum was used for inflammatory marker analysis using ELISA. Behavioral studies measured cognitive changes, while histopathology, immunohistochemistry, and western blot analysis of hippocampal samples measured protein changes. The behavioral studies showed a significant decrease in cognitive function associated with gut dysbiosis in HFHCD and HFD animals. Gut dysbiosis was associated with intestinal inflammation and increased intestinal permeability, followed by systemic and neuroinflammatory changes. Molecular signaling studies showed the involvement of AIM2 inflammasome and cGAS-STING signaling pathways in neurodegeneration for HFHCD animals. Administration of probiotics restored the above processes and prevented gut dysbiosis-associated memory decline in mice. The study shows that alteration in microbial composition due to prolonged HFHCD could contribute to intestinal inflammation and increased intestinal permeability, facilitating the translocation of microbial toxins like LPS, leading to systemic inflammation, which eventually leads to neuroinflammation and neurodegeneration.

Lessons Learned From Epidemiology of Type 2 Diabetes in South Asians: Kelly West Award Lecture 2024.

South Asia has high prevalence rates of type 2 diabetes (T2D). Until the 1990s, the prevalence of T2D within South Asia was low but much higher in the South Asian diaspora living abroad. Today, high prevalence rates of T2D are reported among those living in South Asia. T2D in South Asians presents with unique clinical features described as the "South Asian phenotype" that include younger age at onset of diabetes than in White Europeans, much lower BMI, hyperinsulinemia and greater insulin resistance, rapid decline in β-cell function resulting in low insulin reserve, low muscle mass, and greater ectopic fat deposition, especially in the liver. Also, prevalence of impaired fasting glucose is higher among South Asians than prevalence of impaired glucose tolerance. Genetic predisposition combined with intrauterine fetal programming (low vitamin B12 intake and high folate intake) increases susceptibility to T2D, from birth. In later life, overnutrition, especially a high carbohydrate intake with refined grains of higher glycemic index, coupled with low physical activity likely triggers the T2D epidemic in South Asians. Additionally, there are emerging risk factors like air pollution. Preventing T2D in South Asians requires a multifactorial approach, including improvements in maternal and fetal nutrition with special reference to vitamin B12 and folate intake, decreasing refined carbohydrate and increasing protein and fiber intake in the diet, increasing physical activity, and control of air pollution. Lessons learned from epidemiology of T2D in South Asians could be useful to other developing countries that are in earlier stages of epidemiological transition.

Effects and function of citric acid on fermentation quality and microbial community in sugarcane tops silage with high and low water-soluble carbohydrate content

Sugarcane tops silage (STS), as a source of roughage for ruminants, is rich in water-soluble carbohydrate (WSC) content, which significantly affects silage quality. Citric acid (CA) is a low-cost natural antimicrobial agent that can inhibit undesirable microbes and improve silage quality. The objectives of this study were to investigate the effects of CA on the chemical composition, fermentation quality, microbial communities, and metabolic pathways of STS with high and low WSC contents before or after aerobic exposure. Fresh sugarcane tops with low-WSC [143.05 g/kg dry matter (DM)] and high-WSC (249.99 g/kg DM) contents were treated with and without CA and then ensiled for 125 days, followed by aerobic exposure for 4, 8, and 16 days. The results showed that high-WSC STS had lower crude protein (CP) content and higher DM, neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents, whether treated with CA or not. CA-inoculated silage exhibited decreased DM loss and enterobacteria (EB) counts compared to the control. High-WSC STS treated with CA had higher WSC content and lower yeast count than those without CA inoculation. During the 0–16 days of aerobic exposure, the propionic acid and butyric acid contents in CA-inoculated silage were almost unchanged and ranged from 0 to 1 g/kg DM. Meanwhile, the ethanol content was almost unchanged and ranged from 0 to 1 g/kg DM only in low-WSC STS, irrespective of CA addition. Before aerobic exposure, CA inoculation decreased the abundances of undesirable microbes (e.g., Clostridium_sensu_stricto_12 and Paecilomyces) and animal pathogens, while amino acid metabolism was lower in high-WSC STS regardless of CA treatment. After aerobic exposure, CA inoculation increased the abundance of bacteria with antibacterial effects, including Paenibacillus and Bacillus. Moreover, the metabolism of energy and nucleotides was lower in high-WSC STS treated with CA, and the animal pathogens was lower in low-WSC STS treated with CA. In conclusion, CA inoculation could be effective in decreasing nutrients loss, improving fermentation quality, inhibiting harmful microorganisms, and modulating the metabolic pathways of microorganisms in STS with high and low WSC contents prior to and after aerobic exposure.

Hepatic and Pancreatic Cellular Response to Early Life Nutritional Mismatch.

To determine the basis for perinatal nutritional mismatch causing metabolic dysfunction associated steatotic liver disease (MASLD) and diabetes mellitus, we examined adult phenotype, hepatic transcriptome, and pancreatic β-islet function. In prenatal caloric restricted rat with intrauterine growth restriction (IUGR) and postnatal exposure to high fat with fructose (HFhf) or high carbohydrate (RC), we investigated male and female IUGR-Hfhf and IUGR-RC, versus HFhf and CON offspring. Males more than females displayed adiposity, glucose intolerance, insulin resistance, hyperlipidemia, hepatomegaly with hepatic steatosis. Male hepatic triglyceride synthesis, de novo lipogenesis genes increased, while female lipolysis, β-oxidation, fatty acid efflux, and FGF21 genes increased. IUGR-HFhf males demonstrated reduced β-islets insulin and humanin, and type1 diabetes mellitus human amniotic fluid increased humanin. Humanin suppression disabled glucose stimulated insulin, ATP production, with apoptotic diminished β-islet viability. Humanin and FGF21 may reverse perinatal nutritional mismatched phenotype, by restoring functional β-islets and preventing MASLD and diabetes mellitus.

Application of Neem and Moringa Seed Oil as Botanical Preservative for Cowpea (<em>Vigna Unguiculata</em> L. Walp)

Purpose: Cowpea, a valuable grain with high protein, carbohydrate, and vitamin content, faces significant post-harvest losses due to insufficient storage, pest infestations, and inefficient processing techniques. Thus, the efficacy of a combination of neem and moringa seed oils in a 1:3 ratio, serving as botanical insecticides, to control Callosobruchus maculatus infestation in five different cowpea varieties was evaluated.Research Method: The extracts were mixed at a ratio of 1:3 neem-moringa seed oils and applied mechanically to cowpea varieties in a concentration of 5.0. They were kept in plastic bowls measuring 17 cm deep by 24 cm wide, covered with muslin cloth for free-flow ventilation, and stored for 360 days.Findings: The result of the data collected at every 60 days showed that the seed extracts were effective in preserving all the cowpea varieties even at 360 days. The results indicate that certain cowpea varieties, such as Ife brown (IFB), were more prone to weevil attacks, while others, like Lobia brown (BGR), shown relatively lower susceptibility. The control samples exhibited a higher number of egg-laying and weevil populations compared to the treated samples.Value: The study revealed that the treatment successfully eradicated the weevil population and prevented new infestations.

Evaluating the Nutritive Value and Quality of Fodder Pea (Pisum arvense L.) Silage Added with Varying Rates of Barley (Hordeum vulgare L.) and Wheat (Triticum aestivum L. Em Thell) Cracks

Silage represents the most effective solution for addressing the deficit in quality, abundance and cost-effectiveness of roughage. The main purpose of silage production is to store fresh herbage material with high nutritional value with minimum nutrient loss. For fodder and silage production, fodder pea is grown in mixtures with cereals in different ratios, but also for the silage from the pure fodder pea using additives. Molasses and crashed cereal grains or cereal can be added to silage material in order to increase its carbohydrate content. The research was conducted to determine nutritive value and silage quality of barley and wheat cracks added at different ratios to fodder pea silage in complete randomized split-plot design with four replications. 100 g withered fodder pea sample without additives and 100 g withered fodder pea sample with 3 g, 6 g, 9 g and 12 g cracked barley were vacuumed in to the 20x26 cm plastic bags and were stored in a dark environment for a period of 45 days to facilitate fermentation at ambient temperatures ranging from 15 to 28°C. Crude protein (%), crude ash (%), dry matter (%), digestible dry matter (DDM, %), dry matter intake (DMI, %), ADF (%), NDF (%), P (%), K (%), Ca (%), Mg (%), total digestible nutrients (TDN), Nel, NEm, Neg, pH, Fleig Score and RFV of pure fodder pea and cracked barley and wheat added silages were determined. According to results, fodder peas, which have a high protein and low carbohydrate content, be ensiled by the addition of high-carbohydrate wheat and barley cracks in order to obtain a quality silage. For this purpose, it is suggested to add at least 6% wheat cracks or 9% barley cracks to fodder pea silage.

Growth and Yield of Sweet Corn Plants (Zea mays L Var saccharata.) Effect of Providing Various Nitrogen Sources and Pruning Techniques

Corn is a vital food component primarily due to its high carbohydrate content. This research examines the effects of various nitrogen sources and pruning methods on the growth and yield of sweet corn. The investigation occurred in Tambon Tunong Village, located in the Dewantara District of North Aceh Regency, and in the Laboratory of the Faculty of Agriculture at Malikussaleh University from February to May 2024. A randomized block design (RAK) was employed for the experimental setup, incorporating two main factors: nitrogen sources (ZA fertilizer, HX Nitro fertilizer, and Urea fertilizer) and pruning methods (No Pruning, Pruning at 45 days after sowing (HST), and Pruning at 55 HST). Nine treatment combinations were evaluated, each replicated three times, resulting in 27 experimental units with 15 plants per unit, culminating in 405 plants. The findings indicated that urea fertilizer at 300 kg/ha produced the most favorable results regarding plant height, stem diameter, cob weight, and overall corn yield (10.79 tons/ha). Additionally, pruning at 45 HST resulted in the highest measurements for stem diameter, leaf count, and corn yield (7.81 tons/ha).

UTILIZATION OF FRUITS AND FLOWERS OF CAPPARIS DECIDUA FOR THE DEVELOPMENT OF VALUE-ADDED JAM

Background: There are many neglected fruits available in Pakistan which elucidated health benefits. These fruits wasted every year without any use. The utilization of these fruits for value-added products can contribute in generating enough resources for country while providing health benefits to consumers. Objective: This study focused on the development and characterization of jam from fruits and flowers of Capparis decidua. Methods: The fruits and flowers were washed with water and then boiled at 100 °C until become soft. Then, softened fruits and flowers were blended until a smooth puree were obtained. 200 g pulp with equal amount of sugar and few drops of citric acid was added in a saucepan and heated until the jam reached to its desired consistency. Results: The result demonstrated that the fruits jam has higher carbohydrate, protein, fiber and ash 58, 37, 14.8 and 3.72 as compared to flower jam, respectively. Sensory ratings indicated that both jams have good acceptable quality and sensory attributes. In consumer effective test, fruit jam was found to be better in texture and flavor while the flower jam was found to be better in color, aroma and overall acceptability as compared to each other. In QDA, fruit jam was found to be better in flavor, texture, aroma and overall acceptance while flowers jam showed better color as compared to each other. Conclusion: Both fruits and flowers of Capparis decidua can be used as a functional ingredient in manufacturing of commercial jams. Further, researches required to explore its potential in other functional food products.

A very low carbohydrate diet improved metabolic profile in congenital generalized lipodystrophy type 4.

A 17-year-old girl presented with recurrent attacks of acute pancreatitis, associated with severe hyperglycemia and hypertriglyceridemia, despite being on intensive insulin therapy for the last 10 years. She had severe acanthosis nigricans, generalized loss of subcutaneous fat and prominent veins over extremities. The serum levels of glucose and triglyceride did not reduce significantly, even with maximally tolerated doses of metformin (2 g), pioglitazone (45 mg) and fenofibrate (160 mg), not uncommonly seen in poor rural families in West Bengal, India. A detailed dietary recall revealed a very high carbohydrate intake (70% of total calorie) with very low protein and fat intake. A switch to a very low carbohydrate (30% of total calorie) diet led to a remarkable improvement in glucose and lipid profiles (the daily insulin requirement came down by 50% and triglyceride level came down to 600 mg/dL from 950 mg/dL). A whole-exome sequencing study confirmed congenital generalized lipodystrophy type 4. A carbohydrate restriction strategy may improve difficult-to-control glycometabolic profile in lipodystrophic subjects on high-carbohydrate diet. Lipodystrophy should be suspected in patient presenting with hyperglycemia, hypertriglyceridemia and low BMI. A very low carbohydrate diet (30% of total daily calorie intake) may significantly improve glucose and lipid profiles in patients with lipoatrophic diabetes. Blood glucose may be the most important initial step to control hypertriglyceridemia and risk of pancreatitis in this group of patients.

Highly selective acetate production from wine lees through acidogenic fermentation

Among winery wastes wine lees have a high, unexplored potential for the production of carboxylic acids and more particularly acetate. In fact, they have a high ethanol and low carbohydrate content which can make thermodynamically feasible the oxidation of ethanol to acetate. In this study, the potential of wine lees for anaerobic acetate production was assessed in batch conditions, 37 °C and pH 5.5. White wine lees (WWL) and red wine lees (RWL) were fermented with and without inoculum, and RWL were also co-fermented with waste activated sludge at 20, 40, 70 and 100 gCOD/L. Endogenous microbiome had the same fermentation performances than the external inoculum in WWL, while it led to almost no carboxylates production in RWL, where the community was dominated by the H2-producer Klebsiella (81.6%). Overall, acetate always represented the majority of carboxylates (58–72% on COD basis). H2 production was low (0.31–6.97 mL H2/g bCODin), thus enabling anaerobic ethanol oxidation to acetate (ΔG = −26.6/-7.4 kJ/mol). In co-fermentation, at 70 and 100 gCOD/L caproate (10.0–16.0%) and heptanoate (1.6–5.4%) appeared, alongside a microbiome enriched in lactate-producers (up to 24.5%). Overall, the high acetate selectivity obtained is promising for biorefinery process coupling.

Hydrological transport and endosperm weakening mechanisms during dormancy release in Tilia henryana seeds.

Seed germination is a pivotal stage in the plant life cycle, with endosperm weakening and radicle elongation serving as crucial prerequisites for successful endospermic seed germination. Tilia henryana seeds exhibit deep dormancy, necessitating a period of 2-3 years to germinate in a natural environment, and the germination rate is extremely low. This study employed morphological and physiological approaches to dynamically analyzing the hydrological mechanism and the endosperm weakening process during the dormancy release of T. henryana seeds. It was found that there was no physiological post-ripening effect of embryos, but there were mechanical and physiological obstacles in endosperm. During the dormancy release process of T. henryana seeds, initial endosperm weakening occurred at the radicle-endosperm interface. In this process, the GA/ABA level is imbalanced along with a continuous decrease in IAA and SA levels. Substantial depletion of storage materials within cells resulted in degradation of endosperm cell contents, forming numerous cavities through which significant amounts of free water entered. As moisture content increased, endosperm hardness gradually decreased to approximately 5N/0.09cm2. Furthermore, the area and content of lignin and cellulose were reduced by 58.91% and 84.49%, respectively, while the hemicellulose and pectin contents were decreased by 72.11% and 83.50%, in that order. Following treatment, the activity of pectin lyase, propectinase, galacturonase, and cellulase was observed to be 5.81, 8.72, 5.96, and 9.43 times higher, respectively, in comparison to their respective activities before treatment. The physiological changes facilitated the rapid rupture of the endosperm cell wall, leading to a transition in cell morphology from palisade-like to irregular and interlocking, thereby further expediting the weakening and cleavage of the endosperm. Additionally, T. henryana seeds exhibited high carbohydrate composition content throughout their dormancy release process, this extensive utilization of storage substances provided energy for radicle elongation and expansion.

Level of dietary practices and frequency of physical activity

Introduction: dietary practices have been a fundamental issue in the area of public health, because they play a vital role in the prevention of deficiency or excess diseases.Objective: this research aims to determine the level of dietary practices and the frequency of physical activity in the province of Santo Domingo de los Tsáchilas - Ecuador 2021.Method: a quantitative, non-experimental, descriptive and correlational, and cross-sectional study was applied with a simple probabilistic sample of 692 inhabitants.Results: the majority of respondents are men (59.5%) and single (68.8%). Protein consumption is mostly medium (65.5%), while carbohydrate consumption is high (76.3%), which can increase the risk of being overweight. Vegetable consumption is medium (55.4%), and dairy consumption is also mostly medium (44.4%). Liquid consumption is similar between medium (41.5%) and high (42.1%) levels. As for fruit, 52.6% consume it regularly. Healthy eating habits are medium (40.9%), and 45.2% engage in physical activity, although there is a notable risk of sedentary lifestyle (36.7%).Conclusions: The study shows a male and single profile among the respondents. Although protein and vegetable intake is medium, high carbohydrate consumption and low fluid intake pose risks of overweight and dehydration. Most consume fruit, but eating habits are medium. It is recommended to promote healthier habits and increase physical activity

Assessment of risk of dental caries among middle school children based on carbohydrate intake in Pune: Cross-sectional study

Introduction: This study aimed to evaluate the relationship between carbohydrate intake and the risk of dental caries among middle school children aged 11-15 years in Pune, Maharashtra, India. Objective: Dental caries is a prevalent oral disease, particularly in this age group, as most permanent teeth have erupted, making them vulnerable to decay if proper oral hygiene and dietary habits are not maintained. Methodology: A cross-sectional study was conducted among 197 students from various schools in Pune. Data were collected through questionnaires and clinical examinations done through DMFT & DMFS index, focusing on dietary habits, brushing frequency, and oral hygiene practices. Result: The result of the study shows that there is a significant differences in oral hygiene practices based on intake of carbohydrate, with notable correlation between high carbohydrate intake and increased DMFT and DMFS scores. Specifically, children consuming >20% carbohydrates exhibited significantly higher mean DMFT (3.57 + 3.64) and DMFS mean scores (8.71 + 11.41) compared to those with lower carbohydrate intake <20% had low DMFT mean (1.98+1.57) and DMFS mean (3.07+2.99) Conclusion: The study shows that Children consuming more carbohydrates diet showed more dental caries compared to those with less carbohydrate intake

Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (Micropterus salmoides) Fed on High Dietary Carbohydrates

In order to mitigate the adverse effects of high carbohydrates on largemouth bass and to investigate the feasibility of LA as a feed additive, the present study observed the effects of added α-lipoic acid (LA) on growth performance, glucose metabolism and immunity in largemouth bass fed on high dietary carbohydrates (10% α-cassava starch inclusion). A total of 315 juvenile largemouth bass (initial body weight, 5.09 ± 0.10 g) were divided into nine tanks (800 L) (upper radius 0.65 m × lower radius 0.5 m × height 1 m), with each holding 35 fish. Three iso-nitrogenous and iso-lipidic diets supplementing with 0 g/kg, 0.5 g/kg and 1 g/kg LA (LA0, LA500, LA1000) were designed to feed juvenile largemouth bass on a satiation diet twice daily for eight weeks with each diet feeding to triplicate groups. The results indicated that the performance in growth was significantly enhanced by the addition of dietary LA (p < 0.05). Meanwhile, hepatic glycogen content was significantly reduced (p < 0.05), and the expression of genes relating to insulin pathway and glycolysis significantly increased with LA inclusion (p < 0.05). The relative expression of insulin receptor a (ira) in the LA500 group was the highest, while the relative expression of glycerol kinase (gk), phosphofructokinase liver type (pfkl) and phosphoenolpyruvate carboxykinase (pepck) was the highest in the LA1000 group (p < 0.05). In addition, LA supplementation significantly increased the activity of lysozyme, which reached its maximum value in the LA500 group (p < 0.05). LA supplementation also promoted the expression of genes relating to anti-inflammatory and inhibited the expression of pro-inflammatory related genes (p < 0.05). Above all, the dietary addition of LA could improve performance in growth, alleviated hepatic glycogen deposition, and improved the immunity function of largemouth bass fed on high dietary carbohydrates. This provides us with ideas to mitigate the adverse effects of high carbohydrates on largemouth bass in actual production and provides a basis for the application of LA in aquatic biology.

Dietary habits as risk factor of gallstone disease in Benghazi, Libya: a case control study

Background: Gallstone disease is one of the most common diseases in developed and developing countries; recent studies have expressed evidence in the context of the role of nutrition in the gallstone disease etiology. The most common risk factors for gallstones formations are old age, female gender, obesity and diabetes mellitus. The dietary risk factors, includes consumption of carbohydrates, fats, proteins and dietary products, Moreover, healthy dietary pattern, coffee, rich sources of vitamins as fruits and vegetables do have protection role of gallstones. Our aim is to determine the dietary habits as a risk factor of gallstone diseases admitted in Al-Jala Hospital. Methods: An analytic case control study the data collected from documents of gall bladder stone (GBS) cases that have presented in surgical department and controls from other departments at Al Jala Hospital of Benghazi-Libya between January 2023 and December 2023. Results: The results of the study showed that cases diagnosed with GBS had high dietary intake of carbohydrates, fat, dairy products and meats and whereas had low dietary intake of vegetables and fruits which highly containing fibers, vitamins and minerals in contrast controls had low intake of carbohydrates, fat, dairy products and meats and whereas had high dietary intake of vegetables and fruit (vitamins and minerals) and caffeine. Conclusions: The study concluded that dietary risk factors that contributing to the high prevalence of gall stone formation were because of high intake of high carbohydrates, proteins and fatty diets and low intake of dietary fiber vitamins and minerals as well as caffeine also cases ender and high body mass index (BMI) play important role in gall stone formation.

Assessment and Prediction of the Food Production Capacity and Consumption Gap in Arid Oasis Metropolitan Area.

Food security is the foundation of sustainable human development, and the balance between food supply and demand in urban areas is highly important for promoting residents' health and the sustainable development of cities. This paper takes the Urumqi Metropolitan Area (UMA), a typical oasis urban area, as the study area and uses the food production-demand gap indicator to assess the balance of food production and consumption in the region from 2000 to 2020 and projects food demand in 2030-2060. The results show, first, that residents' food consumption is characterized by high carbohydrate, protein, and fat consumption, and that this put more pressure on food production. Second, different food consumption structures will have different impacts on food production, and the local food production capacity in UMA falls short of ensuring a balanced nutritional structure for residents. Third, food demand increases significantly in 2030-2060, and the pressure of population consumption structure on food production is much greater than that of population growth. Considering the environmental effects of food transportation and the loss of food nutrients, on the production side, the construction of the UMA should be accelerated by including Qitai County and Jimsar County in the UMA's planning scope, strengthening city-regional connections, and improving the local food production and supply capacity of surrounding areas. On the consumption side, regional dietary guidelines should be developed based on local dietary culture and agricultural production conditions to help guide residents to adjust their dietary structures, thereby alleviating pressure on local food demand. Such measures are crucial for ensuring sufficient food supply and promoting balanced nutrition among the population.

Effects of dietary carbohydrate and lipid levels on growth performance, hepatic histology and antioxidant capacity and flesh texture of mandarin fish (Siniperca chuatsi).

In this study, nine isonitrogenous experimental diets containing graded levels of carbohydrates (40 g/kg, 80 g/kg and 120 g/kg) and crude lipids (80 g/kg, 120 g/kg and 160 g/kg) were formulated in a two-factor (3 × 3) orthogonal design. A total of 945 mandarin fish with similar body weights were randomly assigned to twenty-seven tanks, and the experiment diets were fed to triplicate tanks twice daily for 10 weeks. Results showed that different dietary treatments did not significantly affect the survival rate and growth performance of mandarin fish. However, high dietary lipid and carbohydrate levels significantly decreased the protein content of the whole body and muscle of cultured fish. The lipid content of the whole body, liver and muscle all significantly increased with increasing levels of dietary lipid, while only liver lipid level was significantly affected by dietary carbohydrate level. Hepatic glycogen content increased significantly with increasing dietary carbohydrate levels. As to liver antioxidant capacity, malondialdehyde content increased significantly with increasing dietary lipid or carbohydrate content, and catalase activity showed an opposite trend. Superoxide dismutase activity increased significantly with increasing levels of dietary lipid but decreased first and then increased with increasing dietary carbohydrate levels. Additionally, the increase in both dietary lipid and carbohydrate levels resulted in a significant reduction in muscle hardness. Muscle chewiness, gumminess and shear force were only affected by dietary lipid levels and decreased significantly with increasing dietary lipid levels. In conclusion, considering all the results, the appropriate dietary lipids and carbohydrate levels for mandarin fish were 120 g/kg and 80 g/kg, respectively.

A linker histone acts as a transcription factor to orchestrate malic acid accumulation in apple in response to sorbitol.

High carbohydrate availability promotes malic acid accumulation in fleshy fruits, but the underlying mechanism is not known. Here, we show that antisense repression of ALDOSE-6-PHOSPHATE REDUCTASE in apple (Malus domestica) decreases the concentrations of sorbitol and malate and the transcript levels of several genes involved in vacuolar malate transport, including the aluminum-activated malate transporter (ALMT) gene MdALMT9 (Ma1), the P-ATPase gene MdPH5, the MYB transcription factor gene MdMYB73, and the cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1, in fruit and leaves. We identified a linker histone H1 variant, MdH1.1, which complements the Arabidopsis (Arabidopsis thaliana) H1 deficient mutant and functions as a transcription factor. MdH1.1 activates MdMYB73, MdCIbHLH1, and MdPH5 expression by directly binding to their promoters. MdMYB73, in return, binds to the promoter of MdH1.1 to enhance its transcription. This MdH1.1-MdMYB73 feedback loop responds to sorbitol, regulating Ma1 expression. Antisense suppression of either MdH1.1 or MdMYB73 expression significantly decreases whereas overexpression increases Ma1 expression and malate accumulation. These findings demonstrate that MdH1.1, in addition to being an architectural protein for chromatin structure, operates as a transcription factor orchestrating malic acid accumulation in response to sorbitol, revealing how sugar signaling modulates vacuolar malate transport via a linker histone in plants.

Potential Utilization of Taro Yam as Food in Ngada District

Taro (Colocasia esculenta L.) is a tuberous plant called bonggol that grows underground. The high carbohydrate content of taro makes it a top choice as a staple food when looking for alternatives to rice. Taro has great potential to be processed into various food products to help prevent various nutritional problems and generate additional income for local communities. Ngada Regency is one of the districts that has its own natural strengths and is also rich in natural resources such as local food, one of which is taro yam. Quantitative research was used in this study. Data were collected in the form of secondary data or data obtained from books, journals, newspapers, statistical data and other sources. The technique used for this research study is literature study. Ngada Regency has considerable potential for taro yams. Almost all of Ngada Regency cultivates taro yams. However, taro yam processing in Ngada Regency is still quite simple, such as steaming, frying, making compote and animal feed. The people of Ngada Regency certainly have great potential in processing taro yams into industrial raw materials and materials for food needs, such as turning taro yams into taro rice and taro flour. This needs special attention from the local government in empowering the natural resources owned, to open up business potential and encourage job creation for the community, as well as MSME businesses. Ngada Regency is the third largest district on Flores Island in terms of taro yam cultivation, with 2,916 taro yams produced.

BIOTECHNOLOGICAL AND ENVIRONMENTAL EDUCATION POTENTIAL OF Inga edulis Martius: SEED COLLECTED IN THE AMAZON - BRAZIL

Article highlights- Inga edulis offer potential for biotechnological and environmental applications.- High protein and carbohydrate content in seeds support diverse agro-industrial uses.- edulis seeds enhance environmental education via forest nurseries schools.- Biotechnological potential of I. edulis seeds extends to ecosystem services. AbstractInga edulis Martius is a native species of the Amazon Forest with great potential for urban afforestation in the cities of the Brazilian Amazon and widely used for the recovery of degraded areas. It is commonly cultivated by the Amazonian population for its edible fruit, quality wood, and excellent agroforestry components. This study aimed to select the matrices and obtain information about the behavior of the seeds in terms of drying, biometry, purity and germination, and chemical composition. The seeds collected in the environmental education action were recognized about potentialities of use for biotechnological products and environmental protection. The seed exploitation of this species is attractive due to its composition (protein and carbohydrates) and forest integration. I. edulis seedlings in the schools contribute to environmental education as a sustainable practice with biotechnological potential for the Amazon region.