High Amounts Research Articles

κ-Carrageenan improves the gelation capacity of Antarctic krill myofibrillar proteins through covalent and noncovalent interactions

Antarctic krill is a consumption resource with great exploitation potential. Previous study has found that carrageenan contributed to the improvement of texture characteristics of Antarctic krill surimi, but the specific synergistic mechanism remains unclear. This study aimed to evaluate and map the synergistic effect of κ-carrageenan on the quality properties of the myofibrillar protein complex gels of Antarctic krill and its potential modification sites. The analysis of physical quality, structure and molecular force showed that the incorporation of κCG induced the unfolding of myofibrillar proteins, resulting in alterations in the spatial conformation of the proteins, exposure of side-chain groups, and enhancement of intramolecular or intermolecular interactions in the gel system, which increased crosslinking of the MP-κCG composite gel networks. The higher degree of cross-linking facilitated the formation of denser network structures with higher storage and loss moduli, ultimately leading to improved gel quality properties. Notably, κCG plays a dual role in the gel network: it can not only support and stabilize the network structure of gel protein by filling the gaps, but also directly participate in the formation of gels by non-covalent and covalent interactions. Through the identification of the potential modification sites of κCG on Antarctic krill proteins, it was found that the main modified protein was myosin heavy chain and actin. Furthermore, lysine, arginine, threonine, serine and histidine were found to have high adduction quantities and reaction efficiencies. These findings are helpful to guide the quality control of Antarctic krill meat in the application process.

Contamination and pesticide multiresidue analysis in cotton production systems in Benin

High quantity of chemical pesticides spraying in conventional cotton production systems may affect negatively natural ecosystems components. This study examined the contamination levels of soil in Benin's cotton production system in West Africa by residues of pesticides used in cotton farming. One hundred and fourteen soil samples from six conventional and organic cotton production systems were collected and analysed. Multi-class pesticide residues were extracted from the soil samples, and analysed using respectively modified QuEChERS extraction and LC–MS/MS chromatography methods. Results showed an overall soil contamination to emamectin benzoate (18.5 μg/kg), imidacloprid (116.3 μg/kg), profenofos (12.7 μg/kg), acetamiprid (10.8 μg/kg), triasophos (12.6 μg/kg), abamectin (14.3 μg/kg), and deltamethrin (10.4 μg/kg). Especially, emamectin benzoate was detected with a high contamination in Banikoara district (18.5 μg/kg). Nevertheless, these contaminations were not deemed threatening from a toxicological perspective. Soils in conventional cotton cultivation displayed lower organic matter levels but higher concentrations of pesticides, whereas soils in organic cotton cultivation were less degraded and contaminated. Consequently, it is imperative to conduct environmental risk assessments and monitor key pesticide metabolites to establish sustainable cotton production systems in Benin.

Visualizing the spatial distribution of ustalic acid in the fruiting body of Tricholoma kakishimeji.

Imaging mass spectrometry (IMS) was conducted for the first time using ustalic acid (UA) and the fruiting body of Tricholoma kakishimeji to localize mushroom toxins. The mushroom materials were systematically collected in Japan, and analysis of the cross sections of the materials at a resolution of 120μm using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-IMS) revealed the localization of UA and its biogenically related metabolites. MALDI-IMS confirmed that UA was predominantly located on the entire surface of the fruiting body and accumulated in higher amounts in younger fruiting bodies than in mature ones. UA is the first toxic secondary metabolite in the genus Tricholoma locally identified using IMS in mushrooms.

Effect of incorporating biomass bottom ash and construction and demolition waste powder on the physical-mechanical properties and micro-structure of ternary-blended mortars

Biomass bottom ash (BBA) and construction and demolition waste (CDW) are two waste types whose recycling requires immediate actions due to the high quantities in which they are generated worldwide. Since the cement industry is currently facing a real challenge on a global scale due to the environmental cost implicit in cement production, the objective of this study was to evaluate the possibility of reusing those wastes as an addition in ternary-blended eco-cements with a lower impact than common cements. To achieve this, the chemical, physical, mechanical and micro-structural characterisation of the pastes and mortars manufactured with substitution percentages of 10–40% and 1:1 ratio was carried out. The results showed that the mixture of biomass bottom ash and construction and demolition waste powder in the same proportion had the capacity to fix lime and that the new ternary-blended eco-cements made with 20%, 30% and 40% substitution can be classified as CEM IV/A or CEM IV/B pozzolanic cements pursuant to the EN 197–1 standard. All of the new eco-cements also met the regulatory requirements for initial setting time and soundness, with no workability problems recorded. Finally, the mortars made with cement substitutions up to 20% developed similar or greater compressive strength and presented lower porosity at a later age than the reference mortar, making them suitable for use in construction.

Jasmonates Play an Important Role in Differential Accumulation of Key Oolong Tea Aromas in Two Tea Varieties (Camellia sinensis)

Aroma is an important factor in the measurement of the quality and market value of oolong tea. However, it is hard to develop an oolong tea with good aroma quality using unsuitable tea varieties. To explore the key factors of tea varieties in the formation of oolong tea aromas, the fresh leaves of the Chungui variety (CG, suitable for oolong tea, Camellia sinensis (L.) O. Kuntze) and the Fuyun No. 6 variety (F6, unsuitable for oolong tea, Camellia sinensis (L.) O. Kuntze) were harvested and treated by withering and mechanical stress in order. Then, aroma, transcriptome, and jasmonate (JA) contents, and weighted gene co-expression network analysis (WGCNA), of samples were investigated. The contents of characteristic oolong tea aromas, including indole, (E)-β-ocimene, (E)-nerolidol, α-farnesene, and jasmine lactone, were all accumulated in much higher quantities in the CG variety after withering and mechanical stress. Accordingly, the coding genes of aroma formation synthases TSB2, OCS, NES, AFS, and LOX1, and related genes in MVA, MEP, and ALA pathways, were all much more highly activated. These differential reactions are mainly caused by the higher accumulation of jasmonates, especially methyl jasmonate, a type of important plant signal chemical, in CG after mechanical stress. WGCNA analysis indicated 34 different transcription factors from different families are predicted to be involved in this jasmonate-responsive reaction.

Influence of ocean alkalinity enhancement with olivine or steel slag on a coastal plankton community in Tasmania

Abstract. Ocean alkalinity enhancement (OAE) aims to increase atmospheric CO2 sequestration in the oceans through the acceleration of chemical rock weathering. This could be achieved by grinding rocks containing alkaline minerals and adding the rock powder to the surface ocean where it dissolves and chemically locks CO2 in seawater as bicarbonate. However, CO2 sequestration during dissolution coincides with the release of potentially bioactive chemicals and may induce side effects. Here, we used 53 L microcosms to test how coastal plankton communities from Tasmania respond to OAE with olivine (mainly Mg2SiO4) or steel slag (mainly CaO and Ca(OH)2) as alkalinity sources. Three microcosms were left unperturbed and served as a control, three were enriched with olivine powder (1.9 g L−1), and three were enriched with steel slag powder (0.038 g L−1). Olivine and steel slag powders were of similar grain size. Olivine was added in a higher amount than the steel slag with the aim of compensating for the lower efficiency of olivine to deliver alkalinity over the 3-week experiment. Phytoplankton and zooplankton community responses as well as some biogeochemical parameters were monitored. Olivine and steel slag additions increased total alkalinity by 29 and 361 µmol kg−1, respectively, corresponding to a respective theoretical increase of 0.9 % and 14.8 % of the seawater storage capacity for atmospheric CO2. Olivine and steel slag released silicate nutrients into the seawater, but steel slag released considerably more and also significant amounts of phosphate. After 21 d, no significant difference was found in dissolved iron concentrations (>100 nmol L−1) in the treatments and the control. The slag addition increased dissolved manganese concentrations (771 nmol L−1), while olivine increased dissolved nickel concentrations (37 nmol L−1). There was no significant difference in total chlorophyll-a concentrations between the treatments and the control, likely due to nitrogen limitation of the phytoplankton community. However, flow cytometry results indicated an increase in the cellular abundance of several smaller (∼<20 µm) phytoplankton groups in the olivine treatment. The abundance of larger phytoplankton (∼>20 µm) decreased much more in the control than in the treatments after day 10. Furthermore, the maximum quantum yields of photosystem II (Fv/Fm) were higher in slag and olivine treatments, suggesting that mineral additions increased photosynthetic performance. The zooplankton community composition was also affected, with the most notable changes being observed in the dinoflagellate Noctiluca scintillans and the appendicularian Oikopleura sp. in the olivine treatment. Overall, the steel slag used here was more efficient for CO2 removal with OAE than the olivine over the 3-week timescale of the experiment. Furthermore, the steel slag appeared to induce less change in the plankton community than the olivine when comparing the CO2 removal potential of both minerals with the level of environmental impact that they caused.

Microchimeric cells promote production of rheumatoid arthritis-specific autoantibodies

BackgroundWomen are more likely to develop autoimmune diseases than men. Contribution from microchimerism (Mc) has been proposed, as women naturally acquire Mc from more sources than men because of pregnancy. Women with Rheumatoid Arthritis (RA) who lack RA-associated HLA alleles have been found to harbor Mc with RA-associated HLA alleles in higher amounts than healthy women in prior work. However, an immunological impact of Mc remains to be elucidated. ObjectivesTo test the hypothesis that Mc with RA-risk associated HLA alleles can result in the production of RA-associated autoantibodies, when host genetic risk is absent. MethodsDBA/2 mice are unable to produce RA-specific anti-citrullinated autoantibodies (ACPAs) after immunization with the enzyme peptidyl arginine deiminase (PAD) in a previously developed model. DBA/2 females were mated with C57BL/6 males humanized to express HLA-DR4, which is associated with RA-risk and production of ACPAs, to evaluate DR4+ fetal Mc contribution. Next, DBA/2 females born of heterozygous DR4+/− mothers were evaluated for DR4+ Mc of maternal or littermate origin. Finally, DBA/2 females from DR4+/− mothers were crossed with DR4+ males, to evaluate the contribution of any Mc source to ACPA production. ResultsAfter PAD immunization, between 20 % and 43 % of DBA/2 females (otherwise unable to produce ACPAs) had detectable ACPAs (CCP2 kit) after exposure to sources of Mc with RA-associated HLA alleles, compared to 0 % of unmated/unexposed DBA/2 females. Further the microchimeric origin of the autoantibodies was confirmed by detecting a C57BL/6-specific immunoglobulin isotype in the DBA/2 response. ConclusionOur study demonstrates that Mc cells can produce “autoantibodies” and points to a role of Mc in the biology of autoimmune diseases, including RA.

Development of a multiplex real-time quantitative reverse-transcription polymerase chain reaction for the detection of four bee viruses

Viruses in the families Dicistroviridae and Iflaviridae are among the main threats to western honey bees (Apis mellifera) and native bee species. Polymerase chain reaction (PCR) is the gold standard for pathogen detection in bees. However, high throughput screening for bee virus infections in singleplex PCR reactions is cumbersome and limited by the high quantities of sample RNA required. Thus, the development of a sensitive and specific multiplex PCR detection method for screening for multiple viruses simultaneously is necessary. Here, we report the development of a one-step multiplex reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay to detect four viruses commonly encountered in pollinator species. The optimized multiplex RT-qPCR protocol described in this study allows simultaneous detection of two dicistroviruses (Israeli acute paralysis virus and Black queen cell virus) and two iflaviruses (Sacbrood virus and Deformed wing virus) with high efficiency and specificity comparable to singleplex detection assays. This assay provides a broad range of detection and quantification, and the results of virus quantification in this study are similar to those performed in other studies using singleplex detection assays. This method will be particularly useful for data generation from small-bodied insect species that yield low amounts of RNA.

Effects of dry and wet ageing on culled goat meat quality

This study aims to evaluate the effects of ageing time (0, 1, 2, 3, 4 and 5 weeks) and of two different ageing techniques (dry and wet ageing) on the rheological, colorimetric, oxidative, volatile, and sensory attributes of meat (hind limbs) sourced from culled Rossa Mediterranea breed goats. Twenty-four goats were involved in the trial. The hind limbs were divided in the two experimental groups: the right ones were subjected to dry ageing and the left ones to wet ageing. Dry-aged (DA) meat showed higher water-holding capacity (WHC) and lower cooking loss (CL) after ageing compared to wet aged (WA) meat (p < 0.05). Among the colorimetric parameters, only lightness showed to be affected by ageing time, decreasing at week 4 (p < 0.05), with lower values in DA then WA (p < 0.05). Warner Bratzler shear force (WBSF) decreased at week 1 of DA and at week 2 of WA (p < 0.05), with no further changes probably due to the water loss during ageing. Hexanal was the most produced volatile compound, with higher amount in DA meat from 1 to 3 weeks of ageing (p < 0.01) The first two weeks of dry ageing led to an enhancement in sensory and instrumental tenderness, along with an improvement in flavour, characterised by a heightened release of aldehydes and ketones. However, it is noteworthy that beyond this initial phase, dry ageing has led to a substantial decline in the overall quality.

Evaluation of physicochemical parameters and heavy metal concentrations in shrimp (&lt;i&gt;Penaeus monodon&lt;/i&gt;), sediment and surrounding water of the Mangrove Swamps, Rivers State, Nigeria

The study focused on the evaluation of the physiochemical and heavy metal concentration in shrimp (P. monodon), sediments and surface water of Ikpukulu, Kalio, and Ogoloma swamps in Okrika Local Government Area, Rivers State, Nigeria. The following parameters were examined: temperature, potential hydrogen, electrical conductivity, salinity, dissolved oxygen, biochemical oxygen demand, total dissolved oxygen, temperature, and the content of heavy metals (Cd, Cu, and Cr) in surface water, sediments, and shrimp. The pH values (p &gt; 0.05) did not significantly differ amongst the stations. The three sampling station readings of pH were below WHO and FEPA (standards). The temperature data from the three stations did not show any significant differences (p &gt; 0.05), but when compared to the standards, all of the readings were much lower. Result from Ogoloma showed that it had lowest electrical conductivity ratings of the three stations, although overall, the values were higher than the norms. At Kalio, the highest Total Dissolved Solids value (9348.6 ± 67 mg/L) was recorded. When compared to the standards, the salinity values of the three stations were noticeably higher. For Biological oxygen demand, there was no significant (p&gt;0.05) difference between the three stations, but comparing the values to the standards they were all significantly (p&gt;0.05) lower. Heavy metal analysis showed that in the sediment, Copper concentration at Ogoloma was significantly (p&gt;0.05) higher among the three metals in the three stations. Copper and chromium concentrations in the three stations were much higher than the standard. Among the three metals found in the water at the three stations, copper had the highest quantities; the content of copper in Ogoloma was noticeably greater than the standard. Of the threelocations, Ogoloma exhibited a noticeably greater concentration of copper for Shrimp. Thefindings highlight the necessity for preventative action by pointing to an increase in heavy metalconcentrations in the Mangrove swamp area.

The role of germination and boiling processes on bioactive properties, fatty acids, phenolic profile and element contents of hemp seeds and oils

In this study, the effect of boiling and germination processes on oil contents, bioactive compounds, fatty acids, phenolic constituents and mineral amounts of hemp seeds was investigated. The oil quantities of hempseeds were assessed to be between 30.69 % (boiled) and 33.17 % (control). The carotenoid quantities of the hem seeds were assessed to be between 0.68 (boiled) and 2.31 µg/g (raw hempseed). The total phenol quantities and antioxidant capacity values of raw (control), boiled and germinated hempseeds were assigned to be between 29.97 (control) and 51.52 mgGAE/100 g (boiled) to 1.39 (control) and 2.46 mmol/kg (boiled), respectively. Total phenol, flavonoid and antioxidant capacity values of germinated and boiled hemp seeds were higher than the control. 3,4-dihydroxybenzoic acid, catechin, rutin, quercetin and cinnamic acid amounts of boiled hempseeds were found at partly high levels compared to raw (control) hempseed. Oleic and linoleic acid quantities of the hempseed oils were assessed to be between 15.83 (control) and 16.61 (germinated) to 57.24 (germinated) and 57.47 % (control), respectively. Phosphorus was established at the highest quantities in all hempseeds, and followed by potassium, magnesium, calcium (except control) sulphur and sodium in descending order. Iron was measured at the highest quantities, and followed by Mn, Zn, and Cu. The protein quantities of the hempseed samples were established to be between 24.97 (control) and 27.44% (boiled).

Itaconic acid inhibits nontuberculous mycobacterial growth in pH dependent manner while 4-octyl-itaconic acid enhances THP-1 clearance of nontuberculous mycobacteria in vitro.

Increasingly prevalent, nontuberculous mycobacteria (NTM) infections affect approximately 20% of people with cystic fibrosis (CF). Previous studies of CF sputum identified lower levels of the host metabolite itaconate in those infected with NTM. Itaconate can inhibit the growth of M. tuberculosis (MTB) in vitro via the inhibition of the glyoxylate cycle enzyme (ICL), but its impact on NTM is unclear. To test itaconic acid's (IA) effect on NTM growth, laboratory and CF clinical strains of Mycobacterium abscessus and Mycobacterium avium were cultured in 7H9 minimal media supplemented with 1-10 mM of IA and short-chain fatty acids (SCFA). M. avium and M. abscessus grew when supplemented with SCFAs, whereas the addition of IA (≥ 10 mM) completely inhibited NTM growth. NTM supplemented with acetate or propionate and 5 mM IA displayed slower growth than NTM cultured with SCFA and ≤ 1 mM of IA. However, IA's inhibition of NTM was pH dependent; as similar and higher quantities (100 mM) of pH adjusted IA (pH 7) did not inhibit growth in vitro, while in an acidic minimal media (pH 6.1), 1 to 5 mM of non-pH adjusted IA inhibited growth. None of the examined isolates displayed the ability to utilize IA as a carbon source, and IA added to M. abscessus isocitrate lyase (ICL) decreased enzymatic activity. Lastly, the addition of cell-permeable 4-octyl itaconate (4-OI) to THP-1 cells enhanced NTM clearance, demonstrating a potential role for IA/itaconate in host defense against NTM infections.

A straightforward technique to obtain genomic DNA from nasal swabs suitable for sheep SNP genotyping analysis

Isolation of high quality and quantity genomic DNA is essential for molecular studies. It is crucial to select a non-invasive and straightforward technique to ensure the efficient collection of DNA, particularly at the farm level. The aim of this study was to determine if nasal swabs are an appropriate biological matrix to obtain good quality genomic DNA suitable for SNP genotyping. In this study, two biological matrices (blood and nasal swabs) were evaluated and compared for the isolation of genomic DNA obtained from 15 female Texel sheep. DNA quality and quantity were assessed using spectrophotometry and gel electrophoreses. Genotype concordance rates were used for comparison. Results showed that the highest concentration mean was obtained from blood samples (159.14 ng/µl), while from nasal swab samples the concentration mean was lower (130.12 ng/µl), but the difference was non-significant. Regarding purity, DNA obtained from nasal swabs presented a higher A260/A280 ratio (1.96), while the one obtained from blood samples was 1.90. Total DNA yield obtained from blood samples (15.91 µg) was significantly higher than the one obtained from nasal swabs (6.51). Blood and nasal swab genotyping concordance rates were high (mean = 0.984). In conclusion, our results indicate that nasal swabs can yield good quality DNA; however, the DNA extraction protocol should be optimized.

Evaluating the Sustainable Development Performance of China’s International Commercial Ports Based on Environmental, Social and Governance Elements

An environmental, social and governance (ESG) evaluation system can focus on the value of enterprises more comprehensively and better scrutinize the development premise of enterprise. As a novel investment concept, both domestic and foreign investors widely acknowledge the significance of ESG. With the implementation of “carbon peak”, “carbon neutral” and other national strategies, an increasing number of transportation enterprises in China’s international commercial ports have started to focus on the role of ESG evaluation. This not only facilitates self-examination and correction within enterprises but also helps in adjusting the strategic direction toward sustainable development. This shift toward ESG evaluation is crucial for promoting environmental sustainability and corporate social responsibility within the transportation industry. In this regard, this study aims to evaluate the sustainable development performance of China’s international commercial ports based on ESG elements. A data envelopment analysis (DEA) is considered to be a non-parametric performance evaluation method that can effectively solve for multi-criteria decision-making units, so this study mainly selects the DEA model for the performance evaluation. This study conducted research to select eight benchmarking companies within the industry and found that efficient units excelled in their ability to complete capacity levels with high quality and quantity at ports. In contrast, less efficient units scored lower in the domain of social responsibility.

Intelligent design of reconfigurable flexible assembly fixture for aircraft panels based on smart composite jig model and knowledge graph

The current demand for aircraft panel assembly fixtures necessitates agile manufacturing solutions. Traditional welding fixtures struggle to meet diverse panel types, high quantities, and short manufacturing cycles. Conversely, reconfigurable flexible assembly fixtures offer universality, efficiency and ease of maintenance but heavily rely on designers' experience, hindering knowledge reuse. Existing research on computer-aided fixture design (CAFD) primarily addresses specialized fixtures, neglecting reconfigurable flexible ones, lacking fixture design knowledge systems. Aiming at the above problems, the study proposes an intelligent design method for the reconfigurable flexible assembly fixture for aircraft panels based on the smart composite jig model (SCJM) and knowledge graph (KG). Firstly, based on the SCJM of the aircraft panel type assembly fixture (PSCJM), a configuration design method is proposed to carry out the multi-level design of reconfigurable assembly fixture to instantiate PSCJM containing fixture configuration rules and a module library based on model definition (MBD). Then, a knowledge retrieval method is proposed to search the suitable fixture design knowledge based on KG, assisting designers in achieving the flexible design of aircraft panel reconfigurable assembly fixtures. Finally, practical engineering design validation confirms the method's feasibility and effectiveness in obtaining the reconfigurable flexible assembly fixture design scheme for aircraft panels.

Recent Advances in the Discovery of SIRT1/2 Inhibitors via Computational Methods: A Perspective.

Sirtuins (SIRTs) are classified as class III histone deacetylases (HDACs), a family of enzymes that catalyze the removal of acetyl groups from the ε-N-acetyl lysine residues of histone proteins, thus counteracting the activity performed by histone acetyltransferares (HATs). Based on their involvement in different biological pathways, ranging from transcription to metabolism and genome stability, SIRT dysregulation was investigated in many diseases, such as cancer, neurodegenerative disorders, diabetes, and cardiovascular and autoimmune diseases. The elucidation of a consistent number of SIRT-ligand complexes helped to steer the identification of novel and more selective modulators. Due to the high diversity and quantity of the structural data thus far available, we reviewed some of the different ligands and structure-based methods that have recently been used to identify new promising SIRT1/2 modulators. The present review is structured into two sections: the first includes a comprehensive perspective of the successful computational approaches related to the discovery of SIRT1/2 inhibitors (SIRTIs); the second section deals with the most interesting SIRTIs that have recently appeared in the literature (from 2017). The data reported here are collected from different databases (SciFinder, Web of Science, Scopus, Google Scholar, and PubMed) using "SIRT", "sirtuin", and "sirtuin inhibitors" as keywords.

The Impact of Deficit Irrigation on the Agronomic Performance and Chemical Composition of Scolymus hispanicus L.

In the current study, the effects of drought stress on the growth and phytochemical profile of Scolymus hispanicus L. (a.k.a. golden thistle) were evaluated. Plants were treated with three irrigation regimes, e.g., plants that received only rainwater (Control; C), deficit irrigation (I1; 50% of field capacity (FC)), and full irrigation (Ι2; 100% of FC). The fresh weight of the rosette of leaves was not negatively impacted by deficit irrigation, whereas root development was severely restrained compared to control and I2 treatments. Drought stress conditions had a positive effect on the nutritional properties of the golden thistle since the treatments of control and deficit irrigation showed the highest content of macronutrients and energy. Oxalic acid was the richest organic acid, especially under the I1 regime. Similarly, α-tocopherol was the only identified vitamin E isoform, whose content was also doubled in I1 treatment. Raffinose, glucose, and sucrose were the most abundant free sugars in amounts that varied among the irrigation treatments, while the total and distinct free sugar content was the highest for the I1 treatment. The most abundant detected fatty acid compounds were α-linolenic acid, followed by palmitic and linoleic acid, with the highest amount being detected in C, I1, and I2 treatments, respectively. Flavonoids were the only class of polyphenols detected in golden thistle leaves, including mostly kaempferol and quercetin derivatives. The greatest antioxidant potency was shown for the control and I1 treatments (for OxHLIA and TBARS methods, respectively). The evaluated leaf samples recorded a varied antimicrobial effect for the different bacterial strains and fungi, whereas no cytotoxic, hepatotoxic, and anti-inflammatory effects against the tested cell lines were recorded. Finally, the mineral content of leaves was significantly affected by the irrigation regime, with Ca, Mg, Cu, and Zn being the highest for the I1 treatment, while the I2 treatment had the highest content of K, Fe, and Mn and the lowest Na content. In conclusion, deficit irrigation showed promising results since it improved the phytochemical content without compromising the fresh weight of leaves, and thus it could be suggested as a sustainable agronomic practice for producing high-added value products without significant constraints in growth development and yield parameters of golden thistle.

Contact area analysis of an asphalt-concrete boundary layer with X-ray computed tomography imaging

This study focusses on the bond between asphalt and concrete. The bond is a decisive factor for the transfer of traffic loads. A lack of bond between the layers can quickly reduce the load-bearing behavior and thus lead to pavement failure. In the literature, the bond between asphalt pavements and concrete substrates is usually analyzed using mechanical test methods. With mechanical test methods, the effects of interlocking, glue adhesion and friction, which have an effect at the layer boundary, are not considered individually. Based on previous findings, the aim of this study is to carry out a precise analysis of the bonding at the layer interface between concrete and asphalt using bitumen emulsion by means of measurements with a high-resolution X-ray computer tomography (CT). To determine the effect of the surface texture of the substrate, the investigations were carried out on milled and smooth surface textures. In addition to the variation of the substrate profiles, the influence of the application quantity of the bitumen emulsions C40 B5-S and C60 BP4-S was analyzed by spraying quantities of 90 g/m² and 180 g/m² bitumen after breaking. A newly developed method was used to determine the effective degree of gluing at the layer boundary, which allowed the bonding efficiency to be determined. The results of the calculated degree of gluing for the individual variants show that the highest degree of gluing of 61.94% is achieved with the smooth texture variant and high application quantity of bitumen emulsion. The lowest degree of gluing of 17.01% was achieved with the milled texture variant without the application of bitumen emulsion. Overall, the results showed that the degree of gluing was increased for both surface textures by applying the bitumen emulsion. Compared to the smooth texture, the milled textures exhibit a lower degree of gluing for both bitumen quantities, which could be explained by the distribution of the bitumen emulsions on the substrate profiles.

Sound power level and spectrum of port sources for environmental noise mapping

Ports are noisy areas that are often located near populated centers, leading to disturbances for citizens. Neglecting these issues can result in health problems and committees that can impede the natural development of port economic activity, which is growing rapidly. Attention to the sector has increased in recent years, but mainly with studies devoted to the general impact on the population. There is currently insufficient information available regarding the inputs required to conduct noise mapping and assess its effects. This is due to the high quantity and diversity of sources present in the port area. Basing on measurements conducted following established and standardized procedures, the present work reports a database of sound power levels, third-octave band spectra, average duration, and source height for various types of equipment, including reach stackers, dock tractors, front lifts, forklifts, transtainers on wheels, container cranes, mobile cranes, and dry load handling scrapers, during their movements and cargo handling activities. Acousticians are expected to benefit from this detailed input data for noise mapping and environmental acoustic impact assessments.

Mechanical, rheological, thermal and morphological properties of pistachio shell powder reinforced / linear low-density polyethylene/polyolefin elastomer biocomposites

In the present study, 20 wt% ENGAGE (a polyolefin elastomer) is melt blended with 80 wt% linear low-density polyethylene (LLDPE) followed by composite preparation with pistachio shell powder (PSP) in various compositions (40 wt% to 70 wt%). The effect of variation of PSP content on mechanical properties is studied. The processability of composites is improved because of the unique processability of ENGAGE. Consequently, filler-containing capability of the LLDPE/ENGAGE (LE82) blend is also enhanced considerably due to extra space generated by amorphous ENGAGE. Again, blending of 20 wt% ENGAGE with 80 wt% LLDPE is helpful for maintaining mechanical properties of LLDPE/ENGAGE/PSP composite in useful range. The rheological study confirms that complex viscosity (η), storage modulus (G’) and loss modulus (G”) remain almost unchanged with the incorporation of a high quantity of PSP into the LE82 blend system for all the composites in the entire frequency range. From the rheological study, it is seen that the thermoplastic nature of the composites is maintained. Thermogravimetric analysis (TGA) and scanning electron microscopy (SEM) show the structural integrity of LE82 blend-based composites revealing the thermal stability of the organic filler and consequently the composites. SEM also shows uniform dispersion of PSP into the LLDPE/ENGAGE blend matrix. Such biocomposite with 70 wt% biodegradable component and reliable mechanical properties is attractive for wide spread applications.