High Vitamin Research Articles

Association between total vitamin C intake and hypothyroidism among Hashimoto thyroiditis: National Health and Nutrition Examination Survey, 2007–2012

Abstract Oxidative stress may be involved in the progression of hypothyroidism in patients with Hashimoto thyroiditis (HT). Vitamin C is a well-known powerful antioxidant. To our knowledge, whether vitamin C intake relates to hypothyroidism in patients with HT remains unclear. In this cross-sectional study based on the National Health and Nutrition Examination Survey, 2007–2012, we aimed to explore the relationship between total vitamin C intake and hypothyroidism in patients with HT, using multivariate logistic regression models and restricted cubic spline analyses. Our results showed a significant negative linear association between total vitamin C intake (log10-transformed data) and hypothyroidism in HT. Compared with those with the lowest quartile of total vitamin C intake (log10-transformed), participants with the highest quartile were at lower odds of having hypothyroidism (adjusted OR 0·40, 95 % CI: 0·18, 0·88, Ptrend = 0·027). This association was consistent in subgroups stratified by sex (Pfor interaction = 0·084) and age (≥ 60 years and < 60 years, Pfor interaction = 0·330). This study revealed that total vitamin C intake was inversely associated with hypothyroidism among individuals with HT, indicating that higher vitamin C intakes (4·57–1258·9 mg/d) may be associated with a lower likelihood of hypothyroidism among HT participants.

Enhanced vitamin B12 production by isolated Bacillus strains with the application of response surface methodology

Abstract Background Vitamin B12 is a crucial B-group vitamin, first isolated from the liver due to its role in combating pernicious anemia. It is distinguished by its unique and complex structure, which makes its chemical synthesis challenging and expensive. Consequently, vitamin B12 is alternatively obtained through microbial fermentations. Molasses, an affordable and safe agro-industrial waste, can be used as a carbon source for vitamin B12 production, offering a cost-effective alternative to expensive sugars in the production medium. Results A total of 87 yeast, actinomycete, and bacterial isolates were screened for vitamin B12 production, with 15 isolates showing high productivity. Bacillus isolates were selected for further analysis using MALDI-TOF and molecular identification. These isolates were identified as four strains of Bacillus subtilis (MZ08, JT10, BY11, and JT17), one strains of Bacillus sp. (CB09), and one strain of Peribacillus acanthi (MZ01). Genetic circuits associated with vitamin B12 production were demonstrated in a closely related strain of Peribacillus acanthi MZ01 strain. Three strains (MZ01, MZ08, and JT17) were selected for further evaluation of vitamin B12 productivity under different sugar types (glucose, sucrose, fructose, lactose, and galactose) and varying inoculum sizes. The inoculum size significantly impacted vitamin B12 production, with an increase from 5 to 10% enhancing yields. The ability of the strains to produce vitamin B12 varied depending on the type of sugar used. Peribacillus acanthi MZ01 strain showed the highest productivity and subsequently, selected for optimizing vitamin B12 production conditions using response surface methodology. Furthermore, the optimized conditions were then applied to molasses-based medium to achieve high vitamin B12 yields by MZ01 strain. Conclusion In this study, Peribacillus acanthi was characterized for the first time as a vitamin B12 producer, demonstrating high productivity among various tested strains. The optimization of production conditions using response surface methodology, further enhanced vitamin B12 yields, showcasing the strain’s efficiency in microbial fermentations. This research also highlights the potential of using molasses as a cost-effective alternative carbon source, significantly reducing production costs.

Assessment of Genetic Diversity in Greengram (Vigna radiata L.) Using Metroglyph Analysis

Pulses like greengram are essential for sustainable agriculture and global food security due to their high protein, complex carbohydrates, fiber, vitamins, and minerals. However, their genetic diversity is underexplored, with existing studies mainly focusing on conventional methods. This study aims to estimate the variability parameters for 16 quantitative characters of greengram to classify the genotypes using Metroglyph analysis and assess genetic divergence to identify divergent parents for future hybridization programs using 45 greengram genotypes using Randomized Block Design replicated thrice. Among 45 genotypes of greengram on the basis of mean performance MGG-351(12.959) followed by MGG-347(43.16 q/ha), VBN -2 (41.12 q/ha), GM-3(40.8 q/ha) and MGG-385(39.8 q/ha) were found superior for grain yield. High GCV and PCV is recorded for Harvest Index and Number of primary branches. High heritability coupled with genetic advance as percent of mean are recorded for number of primary branches, harvest index (%), number of seeds per pod, biological yield per plant, seed index, number of pods per plant, number of clusters per plant. On the basis of Metroglyph analysis, the total index score was varied from 24 (SHAKTI) to 38 (SIKHA) with a mean of 31.84. Among the 45 germplasm lines, the genotypes GM-3, MGG-385, SML-1663 and MGG-371were observed as high yielder and identified for higher index score. Scatter diagram plotted with Plant Height and Harvest Index has revealed that 5 clusters could be distinguished on the basis of morphological variation. Cluster -1 was represented by 24 genotypes, Cluster -2 was represented by 10 genotypes, Cluster-3 was represented by 7 genotypes, Cluster-4 was represented by 3 genotype, Cluster -5 was represented by 1 genotype. From those complexes, the germplasm lines, SIKHA and MGG-371 recorded high index score and fell into different cluster, hence used as parents in hybridization for getting desirable transgressive segregants.

The chromosome-level Elaeagnus mollis genome and transcriptomes provide insights into genome evolution, glycerolipid and vitamin E biosynthesis in seeds

Elaeagnus mollis, which has seeds with high lipid and vitamin E contents, is a valuable woody oil plant with potential for utilization. Currently, the biosynthesis and regulation mechanism of glycerolipids and vitamin E are still unknown in E. mollis. Here, we present the chromosome-level reference genome of E. mollis (scaffold N50: ~40.66Mbp, genome size: ~591.48Mbp) by integrating short-read, long-read, and Hi-C sequencing platforms. A total of 36,796 protein-coding sequences, mainly located on 14 proto-chromosomes, were predicted. Additionally, two whole genome duplication (WGD) events were suggested to have occurred ~54.07 and ~35.06 million years ago (MYA), with Elaeagnaceae plants probably experiencing both WGD events. Furthermore, the long terminal retrotransposons in E. mollis were active ~0.23MYA, and one of them was inferred to insert into coding sequence of the negative regulatory lipid synthesis gene, EMF2. Through transcriptomic and metabonomic analysis, key genes contributing to the high lipid and vitamin E levels of E. mollis seeds were identified, while miRNA regulation was also considered. This comprehensive work on the E. mollis genome not only provides a solid theoretical foundation and experimental basis for the efficient utilization of seed lipids and vitamin E, but also contributes to the exploration of new genetic resources.

Partially Substituting Photosynthetic Photon Flux Density with Far-red Photons Differentially Alters Biomass Accumulation and Photochemical Efficiency of Greenhouse Lettuce

Adding far-red (FR) photons to a constant photosynthetic photon flux density (PPFD) alters photosynthesis, leaf morphology, and biomass accumulation of horticultural plants. However, the influences of partially substituting PPFD with FR photons under the same extended PPFD (ePPFD) on the growth of greenhouse lettuce (Lactuca sativa L.) is still unknown. In this study, loose-leaf lettuce (‘Dasusheng’) grown in a greenhouse in the fall was implemented using six supplementary light treatments including white plus red (WR) LEDs with substitutive FR photon flux density at 0, 10, 30, 50, 70, and 90 µmol·m−2·s−1 under the same ePPFD (WR139, WR129+FR10, WR109+FR30, WR89+FR50, WR69+FR70, and WR49+FR90, respectively). Lettuce grown with natural light only was designated as NL. The results indicated that supplementary light led to lower plant height, thicker leaves, higher biomass accumulation, higher vitamin C content, higher starch content, and higher total soluble sugar content in lettuce compared with those of lettuce grown under NL. Lettuce grown under WR139 resulted in a 40.0% decrease in plant height, 66.8% increase in leaf thickness, and 34.1% increase in shoot fresh weight compared with those of lettuce grown under NL. No significant differences were observed in leaf width, specific leaf area, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, soil plant analysis development value, apparent quantum yield, soluble protein content, and light use efficiency of lettuce among the WR139, WR129+FR10, and WR109+FR30 treatments. Furthermore, decreased trends were observed in the biomass accumulation and maximum net photosynthetic rate of lettuce with the increased substituted FR photon flux density. However, an opposite trend was found in the maximum photochemical quantum yield. In conclusion, partially substituting PPFD with FR photons at 10 to 30 μmol·m−2·s−1 had similar effects on the biomass accumulation and nutritional quality of greenhouse lettuce. The FR photons should be applied with the consideration of photon flux density because the results also demonstrated negative effects of excess substituted FR photons for PPFD.

High prevalence and seasonal Patterns of vitamin D deficiency in children and adolescents in central China: A Three-Year Single-Center study

ObjectiveThis study aimed to determine the prevalence and associated factors of vitamin D deficiency and insufficiency in children and adolescents in Central China. MethodsIn total, 8,248 children and adolescents aged 0–18 years at a children’s hospital from January 2019 to February 2022, were recruited. Their serum vitamin D levels were measured, and monthly meteorological and air pollution data were collected. ResultsThe overall mean serum vitamin D level was 37.7 ± 17.7 ng/mL, and the prevalence of vitamin D deficiency and insufficiency was 17.7 % and 23.4 %, respectively. Children aged 13–18 years had the highest vitamin D deficiency rate (64.1 %) and the lowest sufficiency rate (4.8 %). The incidence of vitamin D deficiency in girls, especially those aged 6–13 years and 13–18 years, was higher compared to boys. The highest prevalence of vitamin D deficiency was observed in winter (23.2 %), whereas the lowest was found in autumn (12.0 %). Vitamin D insufficiency was most prevalent in summer (27.0 %), significantly higher than in other seasons. Girls were more prone than boys to vitamin D deficiency in spring and summer. Stepwise multiple linear regression analysis showed a positive correlation between vitamin D levels, nitrogen dioxide concentrations, and sunshine hours and a negative correlation with particulate matter 2.5 (PM2.5). ConclusionOur study found high vitamin D deficiency among children in central China, especially girls aged 13–18. Strategies should include promoting sunlight, reducing air pollution, and considering supplementation, particularly in spring and summer.

Examination of the Effectiveness of Fruit Thinning in the Case of Idaho and Gala Must Early Apple Varieties

Abstract Apple (Malus domestica Borkh.) is the most important fruit in the EU. The total yield is more than 11 million tons.year-1, 15% of which is that of the early apple varieties. Several papers have already been published on the efficacy of crown and fruit thinning in the 20th century. Nowadays, hybrid varieties and intensive crown types are used in modernized systems. Our research was carried out in a Hungarian orchard during two cultivation periods. We studied two early apple varieties (Idaho, Gala Must) to determine how fruit thinning affected the quantity and quality of fruits. We found that the effectiveness of non-thinned technology is similar or slightly better compared to fruit thinning, after the second harvest. The average yield of Idaho apples per tree per harvest was 35.9 vs. 24.8 kg, while that of Gala Must was 50.8 vs. 59.2 kg (with thinning vs. without thinning). To assess quality, we measured water-soluble sugars, NO3 -, NO2 and vitamin C content. Immediately, after the harvest, a surprisingly high vitamin C content was found in the fruits (4.4 mg.100 g-1 on average), which, however, decomposed after 3 months of storage. We also determined the pathway of the decomposition of vitamin C.

Bone Mineral Density Screening in People With Epilepsy and Intellectual Disability.

Vitamin D measurements and dual energy x-ray absorptiometry (DXA) scans are recommended in people with intellectual disability and/or epilepsy in order to prevent bone-linked harm. The prevalence of vitamin D supplementation and bone mineral density screening were evaluated in 68 people with epilepsy and intellectual disability (EID) and 68 matched controls with epilepsy without intellectual disability. DXA scans were not performed in any of the people with EID but were performed in 11.8% of the people in the control group. People with EID had a higher vitamin D supplementation rate and were treated with more antiepileptic drugs (AEDs) and more AED combinations, including first generation AEDs. Increased awareness of bone health screening in people with epilepsy and especially EID is warranted.

Effect of Analytical Method on Vitamin C Tablet Levels Stored under Different Temperature Conditions

Vitamin C tablets are commonly consumed by the public as antioxidant supplements. Vitamin C is highly susceptible to oxidation, which is accelerated by factors such as heat, light, alkali, enzymes, oxidizers, and the presence of copper and iron catalysts. Oxidation will be inhibited if vitamin C is left in an acidic state or at low temperatures. However, many vitamin C tablets are not stored according to recommended conditions due to inadequate temperature control during storage or distribution, leading to changes in their vitamin C levels. Vitamin C levels can be analyzed using the Iodimetry method and UV-Vis spectrophotometry. This study aims to determine the effect of the analysis method on the content of vitamin C tablets stored at various temperature variations. This is an experimental research. The independent variable of this study is the analytical method of determining vitamin C content and the dependent variable of this research is the content of vitamin tablets stored at low temperature (2-6°C), room temperature (27-30°C), and high temperature (48°C). Quantitative analysis of vitamin C levels was conducted using both iodimetry and UV-Vis spectrophotometry methods. The highest vitamin C content was found in the analysis of levels using the UV-Vis spectrophotometry method. Statistical analysis shows significant differences, indicating that the method of analyzing the levels of vitamin C tablets stored under varying temperature conditions affects the levels of vitamin C tablets.

Contribution of haptoglobin phenotypic variation to the presence of hyperhomocysteinemia in type 2 diabetics with and without angiopathy.

The genetic polymorphism of haptoglobin (Hp) has been associated with several cardiovascular risk factors, but a possible relationship between Hp phenotypic variation and increased levels of homocysteine (Hcy) and cysteine (Cy) is still unknown. The objective of this study is to evaluate the relationship between the Hp polymorphism and hyperhomocysteinemia (HHcy) and hypercysteinemia (HCy) in type 2 diabetics (T2D) with and without angiopathy (AGP). A case-control study was carried out on 293 adults: Group I (GI) - 75 subjects with T2D and AGP; Group II (GII) - 75 subjects with T2D without AGP; Group III (GIII) - 143 controls. Plasma levels of Hcy, Cy and vitamin B6 were measured by high performance liquid chromatography (HPLC) and vitamins B9 and B12 determined by electrochemiluminescence (ECL). The Hp polymorphism was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and peroxidase staining. The results were analyzed in SPSS®, version 26.0 with a significance of 95%. Mean Hcy concentrations were significantly lower in carriers of the Hp2-2 phenotype (6.14 µM; p = 0.046) compared to the other genotypes. The presence of Hp2-1 is associated with an approximately 3.3 times greater probability of occurrence of HHcy (p = 0.015) and 3.7 times greater probability occurrence of HCy (p = 0.021) in T2D with AGP. The presence of the Hp2-1 phenotype is associated with the predisposition of HHcy and HCy in individuals with T2D and AGP, possibly through a positive heterosis mechanism. Carriers of the Hp2-2 phenotype appear to have a greater activation of the transsulfuration pathway in the Hcy cycle and consequent protection for its accumulation.

Achieving clinical remission in asthma with mepolizumab: a subanalysis on vitamin D as a predictor of response

Objective Mepolizumab, an anti-IL-5 monoclonal antibody, has shown promise in reducing exacerbations and steroid dependency in severe eosinophilic asthma. This study aims to evaluate the effectiveness of mepolizumab in achieving clinical remission in asthma over 12 months and explore Vitamin D levels as a predictor of response. Method We assessed Asthma Control Questionnaire (ACQ) scores, spirometry, number of exacerbations, oral corticosteroid (OCS) use, and inhaled corticosteroid (ICS) use in 32 patients, observing significant clinical improvements. Data were collected 1 year prior to starting mepolizumab and one year after starting mepolizumab. Nasal polyps were not seen in all the patients, and computed tomography of para-nasal sinuses are not available for all the patients, so nasal polyps status are not evaluated to avoid any bias. Result Our results indicate that 12 patients achieved clinical remission after starting mepolizumab, with a strong correlation between higher Vitamin D levels and positive treatment outcomes. This suggests that optimizing Vitamin D levels could enhance the response to mepolizumab in asthma patients, and help in achieving better asthma control. Conclusion Mepolizumab is an effective treatment for severe eosinophilic asthma, significantly improving clinical outcomes and reducing corticosteroid use. This study highlights the importance of Vitamin D as a predictor of response to mepolizumab, suggesting that higher Vitamin D levels may enhance treatment efficacy.

Association between dietary vitamin E and osteoporosis in older adults in the United States.

Increased oxidative stress due to aging can lead to increased bone loss. The most abundant form of vitamin E, namely α-tocopherol, has high antioxidant properties and biological activity; however, its effect on osteoporosis has not been well studied in humans. We aimed to investigate the association between dietary vitamin E (α-tocopherol) and osteoporosis among older adults in the United States. This cross-sectional study analyzed data on older adults in the United States aged ≥50 years from the 2007-2010, 2013-2014 and 2017-2020 pre-pandemic cycles of the National Health and Nutrition Examination Survey. Sample-weighted multivariate regression models were used, with adjustments for relevant confounders. This study comprised 5,800 individuals with available data on dietary intake and bone mineral density of hip and spine. The mean participant age was 61.4 (standard deviation, 8.7) years, and approximately 9.9% had osteoporosis. High vitamin E intake was significantly associated with a reduced risk of osteoporosis (odds ratio, 0.96, 95% confidence interval, 0.93-0.98). In addition, there was evidence of interaction between dietary vitamin E and prior fracture on preventing osteoporosis. Our study indicated a linear association between dietary vitamin E levels and osteoporosis in an older population in the United States. Further research is required to explore the potential effects of different forms of vitamin E on osteoporosis.

CRP Levels and Vitamin D as Predictors of Asthma Control: A Data-Driven Approach

Background: Asthma is a chronic inflammatory disease of the airways characterized by episodic exacerbations and airflow obstruction. Elevated C-reactive protein (CRP) levels indicate systemic inflammation and are associated with increased disease severity in asthma. Additionally, vitamin D is thought to modulate immune responses and inflammation, potentially influencing asthma control. This study explores the relationships between CRP levels, vitamin D levels, and asthma control, as measured by the Asthma Control Test (ACT). Objectives: The primary objective of this study was to assess the correlation between CRP levels, vitamin D levels, and asthma control in adult patients with asthma. The study aimed to determine whether CRP and vitamin D could serve as predictive markers for asthma control. Methods: This single-center, prospective observational study enrolled 40 adult patients with confirmed asthma from the outpatient pulmonary clinic of ESIC Government Hospital, Guwahati, Assam, India. Asthma control was evaluated using the ACT, with scores categorized as well-controlled (ACT ≥ 20) or poorly controlled (ACT < 20). Serum CRP and vitamin D levels were measured using the ELISA method. Pearson’s correlation coefficient and ANOVA were employed to analyze the relationships between these variables. Results: The study found a moderate negative correlation between CRP levels and ACT scores (r = -0.58), indicating that higher CRP levels were associated with poorer asthma control. Patients with CRP levels greater than 10 mg/dL had significantly lower mean ACT scores compared to those with lower CRP levels. In contrast, a strong positive correlation was observed between vitamin D levels and ACT scores (r = 0.879), with higher vitamin D levels associated with better asthma control. The ANOVA test revealed significant differences in ACT scores across different vitamin D and CRP categories (p < 0.05). Conclusions: The study suggests that elevated CRP levels are indicative of poorer asthma control, while higher vitamin D levels are associated with better control of asthma. CRP and vitamin D levels may serve as valuable biomarkers for assessing asthma severity and guiding treatment strategies. However, the variability in response to vitamin D supplementation highlighted the need for personalized approaches to asthma management. Further research is required to confirm these findings and explore the underlying mechanisms.

The effect of Capparis spinosa extract on some immunological and physiological variables of mice

Background The pungent taste and numerous health advantages of the caper plant are not to be overlooked. Thanks to its high vitamin content—including B12, niacin, vitamin K, vitamin A, vitamin E, and fiber—it finds widespread usage as both a culinary seasoning and a medicinal herb. Methodology The Soxhlet device was the most efficient and effective way to isolate the plant's active organic ingredient crude. I ground the plant into a powder using a blender. Then, I added 250 mL of ethanol and added 95% after extraction. I extracted the plant within 24 hours and concentrated the mixture using a rotary evaporator. Finally, I frozen it at -20 ° until I needed it. The mice were housed in sanitary cages that met their nutritional, thermal, and ventilation needs. The experiment did not begin until the mice had been allowed to acclimate for one week without treatment. Result The results presented here appear to be descriptive statistics for a number of physiological characteristics measured in mice treated with varying concentrations of Capparis spinosa extract (control, 10%, and 20%). Haemoglobin (Hb), packed cell volume (PCV), red blood cell count (RBC), white blood cell count (WBC), and the total count for each therapy group are the variables that are being considered. In order to compare the control group with the groups treated with varying doses of Capparis spinosa extract, this data sheds light on the central tendency and variability of physiological variables under different treatments. The results showed an increase in some blood variables compared to the control. There is also an increase in the number of white blood cells, which enhances the increase in the first line of defense against pathogenic organisms.

Comparative Study of the Phytonutrients Contents of Three Plants Grown as Vegetables in Burkina Faso

Diets with a high proportion of plants are nutritionally challenging. These food and generally medicinal plants certainly contribute to reducing hunger and mortality from diet-related diseases worldwide. The aim of this study is to assess the nutritional potential of <i>Cleome gynandra</i>, <i>Hibiscus sabdariffa</i> and <i>Corchorus olitorius</i>, three food plants widely consumed in Burkina Faso. The phytonutrient content of these three plants was assessed on a comparative basis. The parameters investigated in this study were: total ash and mineral content, total protein and carbohydrate content, total lipid content, vitamin C and provitamin A content. The results show that <i>Cleome gynandra</i> had the highest protein content at 160.6 ± 0.32 mg EBSA/g. Lipid levels were relatively close for all three leafy vegetables. They ranged from 3.36% to 4.35%, with the highest content obtained with <i>Corchorus olitorius</i>. Carbohydrate content values ranged from 0.05 to 0.15 mg/mg Glucose equivalent. The highest value was found in <i>Hibiscus sabdariffa</i> (0.15 Glucose equivalent mg/mg). The vitamin C contents of these three plants are relatively close, with the highest vitamin C content obtained with <i>Corchorus olitorius</i> (1.91±1.9 ug/mg). Provitamin A levels varied from 0.196 to 0.312 betac equivalent mg/g ES. The highest content was obtained with <i>Cleome gynandra</i>. Zinc, Calcium, Potassium, Iron, Magnesium and Sodium are also present in all three plants, with varying levels. <i>Cleome gynandra</i> stands out with higher levels of Zinc (59.79 mg/kg), Calcium (9517.5 mg/kg), Potassium (5817.5 mg/kg) and Iron (212.1 mg/kg). These different values justify <i>cleome gylandra</i>'s highest total ash content. These edible plants are therefore rich in phytonutrients, and their consumption could help ensure good health and prevent various chronic diseases.

Extraction of bioactive components from date palm waste, various extraction processes and their applications: A review

Dates are vital sources of nutrients and bioactive components which are widely consumed throughout the world. Agro-industrial waste, such as date palm waste, has been recognized as a potential candidate of bioactive chemicals and essential oils for utilization in food, medicine, and cosmetics. Date fruit and seed are well-known for their nutritional worth, which includes high sugar, vitamins, and mineral content that includes potassium and magnesium. Presence of phenolic compounds diverges the attention of researchers towards efficient extraction processes. Various traditional e.g., soxhlet, maceration, infusion, and digesting and advanced extraction techniques, i.e., pressurised fluid extraction (PFE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), and supercritical CO2 extraction (SC-CO2) have been discussed briefly to extract bioactive components for instance flavonoids, tocopherols, tannins, carotenoids, tocotrienols and hydroxybenzoic acid from date palm fruit, seed, and leaves. The findings reveal that the higher total phenolic content in a shorter time can be obtained by combining ultrasonic with PLE or SFE extraction techniques. These bioactive components can be used in the food and pharmaceutical industries for pre-treatment of certain ailments. Moreover, the natural bioactive components with potential therapeutic benefits, such as non-starch polysaccharides and selenium. The purpose of this study is to highlight the advances achieved in understanding bioactive components e.g., phenolic compounds, flavonoids, tannins hydroxycinnamic, hydroxybenzoic and bio-oil of date palm fruits, including their synthesis, probable functions, and health advantages, to find prospective uses for date-derived materials in the cosmetics, pharmaceutical and food sector.

In Vitro Functional Properties of Rosehips from 'Aurora' Edible Garden Rose's Collection.

Although they have been extensively studied in many species of the genus Rosa L., garden roses' hips have largely been overlooked. To investigate their potential use in the food industry, this study evaluated five cultivars from 'Aurora' collection: 'Purple Aurora', 'Berry Bush Aurora', 'Aromatic Aurora', 'Butterfly Aurora', and 'Rugose White Aurora'. Morphological characterization, along with the assessment of the phenolic profile, vitamin C levels, and biological activities-including antioxidant and neuroprotective effects-was conducted. The fruit mass reached 5.15 g, while the mesocarp mass ranged from 3 to 4 g, resulting in a mesocarp-to-fruit ratio of over 75%. The total phenolic content ranged from 37.1 to 63.9 mg GAE/g de, while total flavonoids were present in amounts from 0.85 to 2.14 mg QE/g de. Rosehip extracts from four out of five cultivars exhibited a very high vitamin C content, reaching 2384 µg/g fw. Quinic acid and 31 phenolic compounds were found in the rosehip extract of at least one cultivar. Results indicated 'Aurora' rosehips have potent antioxidant properties and a moderate inhibitory effect on acetylcholinesterase, highlighting their potential as a source of functional food. Additional research is needed to fully leverage these benefits and establish garden rosehips as a viable alternative to synthetic antioxidants.

Associations between Circulating Biomarkers of One-Carbon Metabolism and Mitochondrial D-Loop Region Methylation Levels.

One-carbon metabolism is a critical pathway for epigenetic mechanisms. Circulating biomarkers of one-carbon metabolism have been associated with changes in nuclear DNA methylation levels in individuals affected by age-related diseases. More and more studies are showing that even mitochondrial DNA (mtDNA) could be methylated. In particular, methylation of the mitochondrial displacement (D-loop) region modulates the gene expression and replication of mtDNA and, when altered, can contribute to the development of human illnesses. However, no study until now has demonstrated an association between circulating biomarkers of one-carbon metabolism and D-loop methylation levels. In the study presented herein, we searched for associations between circulating one-carbon metabolism biomarkers, including folate, homocysteine, and vitamin B12, and the methylation levels of the D-loop region in DNA obtained from the peripheral blood of 94 elderly voluntary subjects. We observed a positive correlation between D-loop methylation and vitamin B12 (r = 0.21; p = 0.03), while no significant correlation was observed with folate (r = 0.02; p = 0.80) or homocysteine levels (r = 0.02; p = 0.82). Moreover, D-loop methylation was increased in individuals with high vitamin B12 levels compared to those with normal vitamin B12 levels (p = 0.04). This is the first study suggesting an association between vitamin B12 circulating levels and mtDNA methylation in human subjects. Given the potential implications of altered one-carbon metabolism and mitochondrial epigenetics in human diseases, a deeper understanding of their interaction could inspire novel interventions with beneficial effects for human health.

Pancreatic Enzyme Replacement Leads to Increased Vitamin D Uptake in Patients Undergoing Sleeve-gastrectomy — A Prospective, Monocentric Trial

PurposeMetabolic and bariatric surgery (MBS) is often considered to be associated with macro- and micronutrient deficiency. A possible treatment option can be the implementation of pancreatic enzyme replacement (PERT) and may lead to better outcomes. We designed a prospective trial investigating the possible impact of PERT in patients undergoing MBS at a high-volume center.Materials and MethodsA prospective two-arm randomized controlled trial was conducted on patients who underwent either sleeve gastrectomy or gastric bypass procedures at a high-volume center. Patients underwent bariatric surgery and follow-up examinations at 3, 6, and 12 months after surgery. Patients were stratified either to the treatment group with PERT or to the control group. The primary endpoint of the study was a change in BMI. Lab testing was carried out to measure secondary endpoints, including albumin and vitamin D levels.ResultsOverall, 204 patients were enrolled. Due to missing follow-ups, surgical complications, and side effects due to Kreon medication, 65 were excluded. Analysis of primary endpoints indicates that PERT does not lead to slower weight loss or BMI reduction. Analysis of secondary endpoints showed significantly better vitamin D levels in patients undergoing MBS and PERT. No statistical difference was seen regarding albumin. In both arms, fatty liver disease improved. Quality of life is positively judged as comparable by patients in both groups.ConclusionHerein, we show an association between PERT and higher vitamin D levels in patients undergoing MBS. An optimized enzymatic environment due to PERT may therefore result in higher vitamin D levels and may improve clinical outcomes in patients undergoing MBS.Graphical

6683 The Interrelationship of Baseline Vitamin K and Vitamin D Status on Incident Fractures: Results from the VITamin D and OmegA-3 TriaL (VITAL)

Abstract Disclosure: S.H. Chou: None. S.L. Booth: None. D. Ratnarajah: None. N. Cook: None. G. Kotler: None. X. Fu: None. J. Buring: None. J.E. Manson: None. M.S. LeBoff: None. Vitamin D and vitamin K have interdependent roles in bone metabolism. We recently reported that, in the VITamin D and OmegA-3 TriaL (VITAL), daily supplemental vitamin D (2000 IU/d) vs. placebo did not reduce fracture risk in 25,871 U.S. men (aged ≥50) and women (aged ≥55).[1] While vitamin D stimulates transcription and translation of osteocalcin (OC) and matrix Gla protein (MGP), vitamin K is needed to carboxylate and activate these proteins. Older adults tend to have low vitamin K intakes, and low vitamin K status may attenuate effects of vitamin D on bone. In this VITAL ancillary study, we tested in a case-cohort sample of 1,162 participants with fractures and an age-sex-matched subcohort size of 2,652, whether baseline low vitamin K status, alone or in combination with lower 25(OH) vitamin D level, predicts incident total, non-vertebral, and hip fractures. The mean (±SD) age of participants was 68.3±7.2 years, 61.5% were female, and 81.7% was Non-Hispanic White. Fractures were reported on annual questionnaires and adjudicated through medical record review. To assess vitamin K function, percent undercarboxylated OC (%ucOC) and dephosphorylated undercarboxylated MGP ([dp]ucMGP) were measured in fasting baseline blood samples, with %ucOC calculated from measures of ucOC and total OC. Higher levels of %ucOC and (dp)ucMGP indicate lower vitamin K status. Serum 25(OH)D was measured by LC-MS/MS and vitamin K biomarkers by sandwich ELISAs (IDS-iSYS Multi-Discipline Automated System). Participants with incident fracture had higher baseline vitamin K status, as assessed by (dp)ucMGP levels, but this was not corroborated by %ucOC. Compared to participants with low baseline vitamin K status and baseline 25(OH) vitamin D levels below the median (30.0 ng/mL), participants with higher baseline vitamin K and D status had a similar incidence of total and non-vertebral fractures. For the 91 hip fracture cases, baseline status of vitamin K and D did not predict hip fracture incidence. In this ancillary study, low vitamin K status by (dp)ucMGP and %ucOC, alone or in combination with baseline 25(OH) vitamin D levels below the median, was not associated with increased fracture incidence. Ongoing analyses are assessing whether phylloquinone levels as another vitamin K biomarker predict incident fracture risk. Furthermore, we have also previously shown that the effects of supplemental vitamin D on total and hip fractures were not modified by baseline vitamin K status in VITAL, the largest randomized controlled trial of supplemental vitamin D vs. placebo on fractures. From our findings in VITAL, higher vitamin K status does not protect against fractures. 1. LeBoff MS, et al. NEJM. 2022. Presentation: 6/1/2024