A thorough understanding of immune-oncology and molecular medicine has been vital in the development of cell therapeutics. At the basis of this translational research and its future implementation into a medicinal product, lies the availability of pure and viable cell populations. However, a versatile technology able of multi marker detection and isolation of complex cell types with high purity, viability and throughput is lacking. Currently, FACS and magnetic bead isolation are successfully used but suffer to fulfill all requirements. FACS is costly and difficult to upscale due to the limitation of shear stress, especially fragile, cells can handle. Therefore, magnetic bead isolation is often used as it is gentler but it lacks the multiparametric aspect to isolate more complex cellular profiles. We have developed a gentle sorting mechanism based on a jet flow created by micro vapor bubbles, enabling a closed microfluidic cell isolation platform capable of multiparametric sorting with high viability, purity and throughput.In previous work, we demonstrated high purity and recovery of rare cells and the multiparametric detection capability. Here we demonstrate that this tool can achieve a pure population of CD4+ CD14- cells with high viability without compromising the recovery. These characteristics complete the list of requirements for the phenotyping and isolation of therapeutic cells. On top of the viability also the growth and activation potential of sorted cells is unhampered by comparison to the benchmark gentle magnetic isolation, mostly deployed in the context of cell therapy manufacturing. Our technology allows for the development of a compact system which sets it apart from other efforts intended to create automated cell therapeutic solutions.