A research trial was conducted during Rabi season, 2022 at crop research farm, Department of Agronomy at Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj Uttar Pradesh India. The soil in experimental field was sandy loam in texture, having alkaline reaction (pH 7.7) with very low organic carbon (0.44%), available higher-level N (171.48 kg/ha), P (27.0 kg/ha) and higher level of K (291.2 kg/ha). To evaluate the response of nitrogen and different plant growth regulators on growth and yield of wheat (Triticum aestivum) the experiment was laid out in Randomized Block Design (RBD) with ten treatments each replicated thrice. The treatment consists three levels of nitrogen (120 kg/ha, 140 kg/ha and 160 kg/ha), in combination with two sprays of Plant growth regulators viz. [Chlormequat chloride (0.2%), Tebuconazole (0.1%) and chlormequat chloride (0.2%) + tebuconazole (0.1%)] applied at 40 DAS followed at 55 DAS whose effect was observed on wheat. The results revealed that, higher plant height (98.70 cm), was recorded with treatment nitrogen 160 kg/ha along with Tebuconazole (0.1%). However, maximum number of tillers/running row meter (78.33), maximum dry weight (23.01 g), higher crop growth rate (0.0059 g/m2/day), more number of effective tillers/m2 (271), highest spike length (15.10 cm), maximum number of grains/spike (60.05), higher test weight (39.75 g), maximum grain yield (4.66 t/ha),) and straw yield (6.98 t/ha) were recorded with treatment nitrogen -160 kg/ha along with CCC (0.2%) and Tebuconazole (0.1 %) in wheat. Application of both nitrogen and plant growth regulators improved the growth and yield of wheat significantly.