Higher Teleosts Research Articles

РОЛЬ ЛИПОПРОТЕИНОВ ВЫСОКОЙ ПЛОТНОСТИ В ПОДДЕРЖАНИИ ОСМОТИЧЕСКОГО ГОМЕОСТАЗА У СЕРЕБРЯНОГО КАРАСЯ CARASSIUS AURATUS (Linnaeus, 1758) (CYPRINIDAE)

A hypothesis on the involvement of high-density lipoproteins (HDL) in maintaining osmotic homeostasis in fish was proposed. For its verification, structural reorganizations of serum HDL in the goldfish Carassius auratus L. were investigated under natural conditions (during the seasonal dynamics) and in experiments on the effect of critical salinity. A common algorithm of HDL rearrangements in wild freshwater fish and those acclimated experimentally to critical salinity was revealed. It consists in reversible dissociation and association of HDL particles. Under natural (freshwater) conditions, this algorithm matches to the dynamics of total protein distribution relative to the capillary wall, under experimental conditions to changes in water salinity. This finding implies the possibility of implementing multiple strategies of maintaining osmotic homeostasis with the involvement of HDL in higher teleosts whose blood, in contrast to mammals, lacks a specialized osmotically active protein albumin. The role of HDL as universal metabolic regulators and stabilizers is discussed.

Evolution of dimorphisms of the proteasome subunit beta type 8 gene (PSMB8) in basal ray-finned fish

The proteasome subunit beta type 8 (PSMB8) gene encodes a catalytic subunit of immunoproteasome that plays a central role in the processing of antigenic peptides presented by major histocompatibility complex class I molecules. The A- and F-type alleles defined by the 31st amino acid residue determining cleaving specificity have been identified from ray-finned fish, amphibia, and reptiles. These two types show extremely long-term trans-species polymorphism in Polypteriformes, Cypriniformes, and Salmoniformes, suggesting the presence of very ancient lineages termed A and F. To elucidate the evolution of the PSMB8 dimorphism in basal ray-finned fish, we analyzed Pantodon buchholzi (Osteoglossiformes), seven species of Anguilliformes, and Hypomesus nipponensis (Osmeriformes). Both A and F lineage sequences were identified from P. buchholzi and H. nipponensis, confirming that these two lineages have been conserved by basal ray-finned fish. However, both the A- and F-type alleles found in Anguilliformes species belonged to the F lineage irrespective of their types. This apparently suggests that the A lineage was lost in the common ancestor of Anguilliformes, and recovery of the A type within the F lineage occurred in Anguilliformes. The apparent loss of the F lineage and recovery of the F type within the A lineage have already been reported from tetrapods and higher teleosts. However, this is the first report on the reverse situation and reveals the dynamic evolution of the PSMB8 dimorphism.

Relationships and biogeography of the fossil and living African snakehead fishes (Percomorpha, Channidae, Parachanna)

ABSTRACT The ability to determine relationships of Cenozoic fossil fishes relies heavily on having osteological information from their extant relatives with which to compare the fossil remains. For many higher teleost fishes, these osteological data do not exist. For example, †Parachanna fayumensis, from Eocene and Oligocene deposits of Egypt, was placed in the Recent snakehead genus Parachanna, but in the absence of data from extant members of the genus, this hypothesis could not be tested. Three other fossil channids were each given their own new genera, without an analysis of their relationships to one another or to the living genera. In order to properly test the relationships of these fossil species, the osteological information for living species is needed. This paper documents the osteology of the African snakehead, Parachanna obscura, for the first time. The documentation of the osteology forms the basis of a phylogenetic study of the relationships of the African fossil, †Parachanna fayumensis, with living African snakeheads. This study supports the generic placement of †P. fayumensis as being correct, as well as reciprocal monophyly of the two living snakehead genera and monophyly of the family. Monophyly of the Asian genus Channa and the relationships of the other fossil species need to be further assessed using a broader range of species, but this preliminary study is an essential first step. Apomorphic osteological characters are given for the family and genera, as well as a discussion of the biogeographical relationships of species of Parachanna.

An unexpected loss of domains in the conservative evolution ninth complement component in a higher teleost, Miichthys miiuy

The complement systems of fish are well developed and play an important role in the innate immune response. C9 is the ninth member of complement components, involved in creating the membrane attack complex (MAC). In the present study, a truncated C9 cDNA sequence encoding 461 amino acids was cloned and characterized in the miiuy croaker (Miichthys miiuy). Typical fish C9 molecules have five characteristic modular domains, i.e. TSP1, LDLRA, MACPF, EGF, and a second TSP domain which is absent in mammalian counterparts. While in miiuy croaker, this truncated C9 presents only TSP1, LDLRA and MACPF domains. It is the first time of finding a truncated C9 in teleost components. RT-PCR analysis detected these C9 transcripts among all tissues examined, demonstrating its constitutive expression pattern in healthy fish. The highest levels of transcripts were detected in liver of both healthy and pathogen-infected miiuy croaker. Its constitutive and inducible expression pattern of this truncated C9 in liver is similar to most complement components which belong to the type of acute-phase proteins and are in general of hepatic origin. We cannot exclude the possibility that miiuy croaker presents the typical C9 although it has not yet been found. The molecular evolutionary analysis showed that this truncated C9 of miiuy croaker had a significantly higher omega value comparing with other fish and the positive selection pressure had happened on it after its divergence with other fish.

15-P025 An acquisition of a novel calcium-regulating organ of chloride cells in the skin of higher teleosts during evolution

15-P025 An acquisition of a novel calcium-regulating organ of chloride cells in the skin of higher teleosts during evolution

Diversified chromosomal characteristics in Centropyge fishes (Pomacanthidae, Perciformes)

We studied karyotypes and other chromosomal markers such as C-banded heterochromatin and Ag-stained nucleolus organizer regions (Ag-NORs), in seven Centropyge fishes (Pomacanthidae, Perciformes). These results revealed diversified chromosomal characteristics in Centropyge species. Three species had 2n = 48 chromosomes, whereas four species had 2n = 52 chromosomes. Fundamental numbers showed a large variation from 48 to 82, particularly in the species with 2n = 52 chromosomes. In all the species, Ag-NORs were located in a single chromosome pair and C-bands were mainly distributed in the centromeric regions of most chromosomes, as commonly seen in teleostean fishes. However, these chromosomal markers showed species-specific variations and provided us with useful information that could help us in understanding chromosomal evolution. On the basis of these chromosomal characteristics, we infer the process of chromosomal evolution, which according to us involves an increase in chromosome number from 2n = 48 to 2n = 52 through centric fission or other mechanisms, and in fundamental number through pericentric inversion. In particular, karyotypic evolution involving the increase in chromosome number is an unusual event in the evolution of higher teleostean groups.

Mitochondrial genome and a nuclear gene indicate a novel phylogenetic position of deep-sea tube-eye fish (Stylephoridae)

The rare, monotypic deep-sea fish family Stylephoridae has long been considered a member of the order Lampridiformes (opahs, velifers, ribbonfishes), and no systematic ichthyologist has questioned its placement within the order for over 80 years. Recently three individuals of Stylephorus chordatus were collected from different oceans, and we sequenced the whole mitochondrial genome and a partial nuclear recombination activating gene 1 (RAG1) gene sequences for each specimen. We aligned these sequences with those available from higher teleosts, including representative lampridiforms, and constructed two separate datasets from the sequences. The resulting trees derived from partitioned Bayesian analyses strongly indicated that S. chordatus is not a lampridiform but is closely related to the order Gadiformes (cod and their relatives). Lampridiformes is diagnosed on the basis of four synapomorphies, three of which are correlated with the rare and possibly unique ability to extend both the maxilla and premaxilla as a unit during feeding. Stylephorus also possesses such unique ability, but lacks two and possibly three of the four synapomorphies, suggesting that further morphological analysis is needed. Considering its unique morphologies with no indication of affinities within Gadiformes (or any other presently recognized order), the present results warrant a recognition of the new order for S. chordatus in fish systematics.

Phylogenetic position of tetraodontiform fishes within the higher teleosts: Bayesian inferences based on 44 whole mitochondrial genome sequences

Tetraodontiformes includes approximately 350 species assigned to nine families, sharing several reduced morphological features of higher teleosts. The order has been accepted as a monophyletic group by many authors, although several alternative hypotheses exist regarding its phylogenetic position within the higher teleosts. To date, acanthuroids, zeiforms, and lophiiforms have been proposed as sister-groups of the tetraodontiforms. The monophyly and sister-group status was investigated using whole mitochondrial genome (mitogenome) sequences from 44 purposefully-chosen species (26 sequences newly-determined during the study) that fully represent the major tetraodontiform lineages plus all the groups that have been hypothesized as being close relatives. Partitioned Bayesian analyses were conducted with the three datasets that comprised concatenated nucleotide sequences from 13 protein-coding genes (with and without, or with RY-coding, 3rd codon positions), plus 22 transfer RNA and two ribosomal RNA genes. The resultant trees were well resolved and largely congruent, with most internal branches being supported by high posterior probabilities. Mitogenomic data strongly supported the monophyly of tetraodontiform fishes, placing them as a sister-group of either Lophiiformes plus Caproidei or Caproidei only. The sister-group relationship between Acanthuroidei and Tetraodontiformes was statistically rejected using Bayes factors. These results were confirmed by a reanalysis of the previously published nuclear RAG1 gene sequences using the Bayesian method. Within the Tetraodontiformes, however, monophylies of the three superfamilies were not recovered and further taxonomic sampling and subsequent efforts should clarify these relationships.

Organization of diencephalon of the sturgeons. Posterior tubercular area. Periventricular and rostral parts

Cytoarchitectonics of periventricular and rostral parts of the posterior tubercular area of diencephalon was studied in four species of the cartilaginous ganoids by using routine Nissl staining and Bielschowski impregnation technique in Viktorov’s modification. The posterior tubercular area in the giant sturgeon Huso huso L., the Kura sturgeon Acipenser guldenstadtii persicus n. Kurensis Belyaeff, the stellate sturgeon Ac. stellatus Pall., and the barbel sturgeon Ac. nudiventris Lov. was shown to have similar structure. Six structures were identified in these areas: the periventricular nucleus, paraventricular organ, nucleus of the paraventricular organ, and posterior tuberal nucleus in the periventricular region and preglomerular medial and lateral nuclei in the rostral region. Both nuclei of the rostral zone are migrated nuclei. Out of nuclei of the posterior tubercle regions, the posterior tuberal and medial preglomerular nuclei are characterized by polymorphism of cellular elements. A conclusion is made that these parts of the posterior tubercular area in the sturgeons considered to be the lower ray-finned fish are comparable with those of the higher teleosts, and even are more differentiated according to some parameters.

Melano-macrophage centres and their role in fish pathology.

Melano-macrophage centres, also known as macrophage aggregates, are distinctive groupings of pigment-containing cells within the tissues of heterothermic vertebrates. In fish they are normally located in the stroma of the haemopoietic tissue of the spleen and the kidney, although in amphibians and reptiles, and some fish, they are also found in the liver. They may also develop in association with chronic inflammatory lesions elsewhere in the body and during ovarian atresia. In higher teleosts, they often exist as complex discrete centres, containing lymphocytes and macrophages, and may be primitive analogues of the germinal centres of lymph nodes. Melano-macrophage centres usually contain a variety of pigments, including melanins, and these increase in range and volume in older fish or in the presence of cachectic disease. Melano-macrophage centres act as focal depositories for resistant intracellular bacteria, from which chronic infections may develop. Iron capture and storage in haemolytic diseases appears to be a primary function, but antigen trapping and presentation to lymphocytes, sequestration of products of cellular degradation and potentially toxic tissue materials, such as melanins, free radicals and catabolic breakdown products are among other functions that have been ascribed. Recent work suggests that they are a site of primary melanogenesis rather than mere storage. Melano-macrophage centres increase in size or frequency in conditions of environmental stress and have been suggested as reliable biomarkers for water quality in terms of both deoxygenation and iatragenic chemical pollution.

Major patterns of higher teleostean phylogenies: a new perspective based on 100 complete mitochondrial DNA sequences

A recent preliminary study using complete mitochondrial DNA sequences from 48 species of teleosts has suggested that higher teleostean phylogenies should be reinvestigated on the basis of more intensive taxonomic sampling. As a second step towards the resolution of higher teleostean phylogenies, which have been described as the “(unresolved) bush at the top of the tree,” we reanalyzed their relationships using mitogenomic data from 100 purposefully chosen species that fully represented all of the higher teleostean orders, except for the Batrachoidiformes. Unweighted and weighted maximum parsimony analyses were conducted with the data set that comprised concatenated nucleotide sequences from 12 protein-coding genes (excluding 3rd codon positions) and 21 transfer RNA (tRNA) genes (stem regions only) from each species. The resultant trees were well resolved and largely congruent, with most internal branches being supported by high statistical values. All major, comprehensive groups above ordinal level as currently defined in higher teleosts (with the exception of the Neoteleostei and several monotypic groups), such as the Eurypterygii, Ctenosquamata, Acanthomorpha, Paracanthopterygii, Acanthopterygii, and Percomorpha, appeared to be nonmonophyletic in the present tree. Such incongruities largely resulted from differences in the placement and/or limits of the orders Ateleopodiformes, Lampridiformes, Polymixiiformes, Ophidiiformes, Lophiiformes, Beryciformes, Stephanoberyciformes, and Zeiformes, long-standing problematic taxa in systematic ichthyology. Of these, the resulting phylogenetic positions of the Ophidiiformes and Lophiiformes were totally unexpected, because, although they have consistently been considered relatively primitive groups within higher teleosts (Paracanthopterygii), they were confidently placed within a crown group of teleosts, herein called the Percomorpha. It should be noted that many unexpected, but highly supported relationships were found within the Percomorpha, being highly promising for the next investigative step towards resolution of this remarkably diversified group of teleosts.

Neuronal organization of the melanin‐concentrating hormone system in primitive actinopterygians: Evolutionary changes leading to teleosts

Hypothalamic melanin-concentrating hormone (MCH) neurones occur in all vertebrates and have an apparent neuromodulatory role. In teleost fish, however, MCH is used also as a neurohypophysial hormone, controlling skin color, and as a hypophysiotrophic peptide. This work describes the central location of immunoreactive MCH perikarya and their projections to the pituitary in a range of ancestral fish to determine the phylogenetic stage when the peptide adopted these roles. In all actinopterygians examined, including polypteriformes, chondrosteans, holosteans, and teleosts, immunoreactive fibers were abundant in the median eminence or, in the case of teleosts, within the pars distalis itself, suggesting MCH acquired a hypophysial regulatory role early in vertebrate evolution. MCH fibers appeared to be absent from the posterior neurohypophysis of the polypteriform Calamoichthys but were evident in this region in the chondrostean Acipensor, the holosteans Lepisosteus and Amia, and all teleosts, suggesting its use as a neurohypophysial hormone. The ability of MCH to induce skin pallor seems to have arisen at a later stage, probably in the preholosteans. This hormonal role coincides with the migration of MCH perikarya away from the ventricular surface and their enlargement into magnocellular neurones. In the higher teleosts, magnocellular hypothalamo-neurohypophysial neurones predominate in size and number, whereas smaller periventricular MCH neurones associated with the paraventricular organ, that are prominent in lampreys, early actinopterygians and tetrapods, are reduced in teleosts. The data suggest that, in teleost fish, earlier functions of the peptide may have become subordinate to its novel pigmentary role.

Comparative analysis of phylogenetic and fishing effects in life history patterns of teleost fishes

The effects of fishing on life history traits and life history strategies of teleost fishes are analysed by a new comparative method that splits traits into an allometric part (size effect), an autoregressive phylogenetic component, and an environmental component (fishing effect). Both intra‐ and inter‐specific variation of age and size at maturity, fecundity, adult size and egg size are analysed by comparing 84 populations of 49 species submitted to various fishing pressures. Two axes of life history diversification are found among teleosts. One is the well‐known slow‐fast continuum separating short‐lived and early maturing species (like Clupeiformes) from longer‐lived species that mature late relative to their size and spawn larger eggs (like salmonids or Scorpaeniformes). An additional strategy involves the schedule of resource allocation to growth and reproduction. Indeterminate growth allows higher teleosts (e.g. Gadiformes) to reach a large size while maturing early and laying small eggs. Increasing fishing pressure decreases age at maturity and egg size, and increases fecundity at maturity, the slope of the fecundity‐length relationship and relative size at maturity. These compensations for higher adult mortality differ among life history strategies. Indeterminate growth is associated with a greater flexibility in resource allocation to growth and reproduction that facilitates greater resilience to fishing mortality.

Observations on the granulocyte peroxidase of teleosts: a phylogenetic perspective

Observations on granulocyte peroxidase of 123 teleosts are reported and correlated with previous studies to put occurrence of fish granulocyte peroxidase in a phylogenetic perspective. Eosinophil peroxidase occurs in archaic groups such as lungfish and sturgeons, but is usually weak and infrequently observed in chondrichthyans, and in teleosts occurs in some elopomorphs, is consistently observed in stomiiforms, is rarely observed and usually weak in acanthopterygians and absent from salmoniforms, scopelomorphs and paracanthopterygians. Peroxidase is absent from lungfish and sturgeon neutrophils, is present in neutrophils of some elopomorphs and clupeimorphs. both of which are basal teleost groups, but in salmoniforms and higher teleosts is consistently observed in neutrophils. although often weak in pleuronectiforms.Loss of eosinophil peroxidase and development of neutrophil peroxidase in teleosts may be due to inefficient phagocytosis and loss of peroxidase with degranulation in eosinophils, being superseded by concentration of peroxidase in phagosomes in neutrophils.

Jaw structures and movements in higher teleostean fishes

All of the diverse jaw structures in higher teleosts appear to be modifications of a single basal type and are treated as such. Only some of the principal variants are discussed. Though the two jaws act as a coordinated unit during feeding, their movements are different. The upper and lower jaws are discussed separately. In the upper jaw the principal concern is with the various types of premaxillary protrusion and with the secondary development in some groups of a rocking premaxilla. For the lower jaw most of the account is devoted to the repeated differentiation of movements in its anterior and posterior sections. The paper concludes with comments on the jaw apparatus as a functional unit and its evolution in higher teleosts.

A possible relationship between aspects of dentition and feeding in the centrarchid and anabantoid fishes

Certain components of dentition — teeth on the third basibranchial in the Centrarchidae and on the parasphenoid in the anabantoids (sensu lato) — are very rare elsewhere in higher teleostean fishes. Though these basibranchial and parasphenoid teeth in the two fish groups are on opposite sides of the oral cavity, it is hypothesized that they both developed as adaptations for gripping a particular category of food items, namely strong-clawed, hard-shelled, active animals that, once within the oral cavity, would try to crawl out again. A corollary to this hypothesis is that higher teleosts with extensive dentition in the central part of the oral cavity have a grasping jaw bite, which, unlike a piercing, shearing, or crushing jaw bite, does not necessarily kill the prey that is taken into the oral cavity.

Genetic control of phosphoglucose isomerase (PGI) isozymes in Anguilliformes: Differential tissue expression of the duplicate PGI loci

Abstract The electrophoretic patterns of phosphoglucose isomerase (PGI) in different tissues of 13 species of Anguilliformes, representing 8 families and 2 suborders, have been studied. As in higher teleosts, the overall presence of two PGI loci is confirmed in this order. A substantially uniform tissue pattern of PGI gene expression has been observed among anguilliform species, but differing, to some extent, from that of higher teleosts. These data agree with the generally accepted phylogenesis of Anguilliformes. An exception to this unvaried pattern is observed for Conger conger from the Tyrrhenian Sea: while the PGI-1 expression is always strong in liver, the PGI-1/PGI-2 proportion is quite variable and consistent with a regulatory polymorphism at the PGI-2 locus.

Lactate Dehydrogenase Isozymes of Gadiform Fishes: Divergent Patterns of Gene Expression Indicate a Heterogeneous Taxon

The lactate dehydrogenase (LDH) isozyme patterns of 35 species of gadiform fishes were analyzed by starch gel electrophoresis and immunochemical procedures. All species exhibited the three expected homopolymers of LDH-A4, B4 and C4. Although the specific properties (absolute net charge and tissue abundance of each isozyme) of the LDHs of each species were unique to that species, certain general features of the LDH patterns characterized most or all species in a given higher taxon (genus, family, suborder). Thus, gadids generally exhibited a highly anodal LDH-A4 homopolymer while ophidiids had one which was nearly isoelectric; macrourids had high levels of LDH-B4 in nearly all tissues while zoarcids only expressed the B4 homopolymer in eye and brain, and so forth. In contrast to the homogeneity of LDH-C4 expression characteristic of nearly all higher teleost groups, a major dichotomy of LDH-C4 patterns was observed within the Gadiformes. The 19 species examined in the suborders Gadoidei and Macrouroidei all possessed a cathodally migrating, liver-predominant C4 isozyme while all sixteen species examined in the suborders Ophidioidei and Zoarcoidei exhibited a highly anodal, eye-specific LDH-C4. Because each of these forms of LDH-C gene expression represents a derived or specialized state relative to the situation in lower bony fishes, and because of the debatable nature of the morphological evidence originally used to justify the inclusion of these four groups in the same order, we suggest that the order as presently defined represents an unnatural grouping. We recommend revision and suggest that the ophidioids and zoarcoids should be removed from the Gadiformes.

Catalase in fish red blood cells

Catalase activity was measured in the red blood cells from four different teleostean species: the milkfish, Chanos chanos; palani, Acanthurus dussumieri; naso, Naso lituratus; and butterfly fish. Chaetodon miliaris. 1. 2. The enzyme activity and associated red cell color changes on exposure to air, correlated with the taxonomic relationships of the species studied. 2. 3. Catalase was detected in all species except the most primitive—the milkfish—which revealed zero to minimal activity. These results are consistent with those of the only other known study (Rabie et al., 1972), on different species. 3. 4. Catalase, therefore, may have evolved with the higher teleosts. 4. 5. The milkfish is suggested as a possible model for a- or hypo-catalasemia in humans.

Phosphoglucose isomerase gene duplication in the bony fishes: an evolutionary history.

Electrophoretic patterns of phosphoglucose isomerase (PGI) in bony fishes provide strong evidence for a model of genetic control by two independent structural gene loci, most likely resulting from a gene duplication. This model is confirmed by a comparison of certain kinetic and molecular properties of the PGI homodimers (PGI-1 and PGI-2) isolated from extracts of the teleost Astyanax mexicanus. In addition, in most higher teleosts examined, the PGI enzymes show a regular pattern of tissue distribution, with PGI-2 predominant in muscle, the heterodimer often strongest in the heart, and PGI-1 predominant in liver and other organs. An examination of 53 species of bony fishes belonging to 38 families indicates a widespread occurrence of duplicate PGI loci and an early origin of the gene duplication, perhaps in the Leptolepiformes. The apparent presence of three PGI loci in trout and goldfish exemplifies how new loci can be incorporated into the genome through polyploidization.