Higher Solvent Exposure Research Articles

C-Terminal Truncated α-Synuclein Fibrils Contain Strongly Twisted β-Sheets.

C-terminal truncations of monomeric wild-type alpha-synuclein (henceforth WT-αS) have been shown to enhance the formation of amyloid aggregates both in vivo and in vitro and have been associated with accelerated progression of Parkinson’s disease (PD). The correlation with PD may not solely be a result of faster aggregation, but also of which fibril polymorphs are preferentially formed when the C-terminal residues are deleted. Considering that different polymorphs are known to result in distinct pathologies, it is important to understand how these truncations affect the organization of αS into fibrils. Here we present high-resolution microscopy and advanced vibrational spectroscopy studies that indicate that the C-terminal truncation variant of αS, lacking residues 109–140 (henceforth referred to as 1–108-αS), forms amyloid fibrils with a distinct structure and morphology. The 1–108-αS fibrils have a unique negative circular dichroism band at ∼230 nm, a feature that differs from the canonical ∼218 nm band usually observed for amyloid fibrils. We show evidence that 1–108-αS fibrils consist of strongly twisted β-sheets with an increased inter-β-sheet distance and a higher solvent exposure than WT-αS fibrils, which is also indicated by the pronounced differences in the 1D-IR (FTIR), 2D-IR, and vibrational circular dichroism spectra. As a result of their distinct β-sheet structure, 1–108-αS fibrils resist incorporation of WT-αS monomers.

Longitudinal Study to Explore Chronic Neuropsychologic Effects on Solvent Exposed Workers

The aim of the study was to examine possible neurotoxic effects on the central nervous system (CNS) in relation to a chronic solvent exposure at the workplace. The collective included 127 workers exposed to solvent mixtures, such as spray painters and printers. They were examined twice by means of a physical examination, neuropsychological testbattery, biological and air monitoring. Major component of the solvents were white spirits in concentrations up to 127 ppm in air and 2,666 microg/l in blood. Single substances were mainly toluene and xylenes in concentrations below current threshold values at the workplace. During the 2 yr interval, the concentrations of solvents decreased, and no significant associations between the neuropsychological tests and the solvent exposure were found. Regarding the whole working history of each participant, we found a significant reduction on information processing velocity and performance in the trailmaking test, as well as more complaints of workers with higher solvent exposure in the past. A slight increase of subjective complaints and a deteriorated power of concentration seems to be associated with chronic solvent exposure.