Considering the relevance of research and publication in the career and professional development and growth of academic librarians especially in Nigerian Universities, this paper was used to find out the factors which contribute to high publication output among librarians in Nigerian University libraries. The main objectives of the study were to find out librarians' motivation for research and publication; find out the factors that contribute to high research productivity among librarians; find out the barriers to research and publication among librarians; and determine the strategies that will enhance research and publication among librarians. The population of the study comprised of 146 librarians of the university libraries in South-East Nigeria. Purposive sampling based on the level of publication output was used to select about 85 librarians as the sample of the study. The data were collected using a questionnaire and analyzed using frequency counts and mean scores and presented in tables. Major findings showed that the desire to earn promotion and to develop one's self among others motivated the librarians to write and publish while collaboration and attendance to conferences among other factors contributed to high publication output. Lack of a research grant and a tight work schedule hinder research and publication while partnering with research funders and issuance of grants enhanced the opportunities for research and publication.