Active food package incorporating an ethanol vapour-controlled release sachet has been known for its efficacies to delay microbial proliferation in fresh fruit and vegetable. High humidity inside the package could be utilized as a stimulus for conditional releases as a means to stabilize the sachet prior to being used. The present research was undertaken to investigate the effects of relative humidity on ethanol vapour release from the hydrophilic film-based sachet. The prototype 4-side sealed sachets were made of either ethylene vinyl acetate (EVA) or laminated film comprising EVA and Nylon/PE (designated as ENP). A gas chromatogram equipped with a flame-ionized detector (FID-GC) was employed to analyze ethanol vapour concentration levels released from both sachet types and accumulated in headspaces of sealed glass beakers having different relative humidity (RH) levels. For a given RH level, the concentrations in the headspaces containing the ENPbased sachets were lower than those containing the EVA-based sachets. Delays of ethanol vapour release up to 24 h were observed in the ENP-based sachet system, whilst these did not occur among EVA-based sachets. Both sachets could release ethanol vapour with faster rates and subsequently higher concentrations accumulated at the very high relative humidity level (90-99% RH), compared to lower RH levels (60-89% RH). However, the release rates and concentration levels accumulated in 60-75% RH were not different from those in 80-89% RH. Extents of water vapour uptake by films were relatively small when the films were kept at the lower RH levels, but these became exponentially increased when the RH levels were ≥90%RH. Experimental data on water vapour uptakes were well predicted by an exponential model (R2 0.92-0.99; and root mean square of errors (RMSE) 0.004-0.054). Overall, experiment findings indicate that the ENP film caused delayed ethanol vapour releases from the sachet. The relative humidity levels had significant effects on the releases from hydrophilic film-based sachets